8 Images. Overview. Product name Description. Predicted to work with: Mouse, Rat, Chicken. Immunogen. Positive control. Properties

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1 Product datasheet Anti-AFAP antibody [2G9] ab Images Overview Product name Description Tested applications Species reactivity Immunogen Anti-AFAP antibody [2G9] Mouse monoclonal [2G9] to AFAP Suitable for: IHC-P, WB Reacts with: Human Predicted to work with: Mouse, Rat, Chicken Recombinant fragment corresponding to Human AFAP aa Produced in E.coli. Sequence: L SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKTRHQ WPSEEASMDL VKDAKICAFL LRKKRFGQWT KLLCVIKDTK LLCYKSSKDQ QPQMELPLQG CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW LKVIKEAYSG CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT CGYLNVLSNS RWRERWCRVK DNKLIFHKDR TDLKTHI Database link: NP_ Positive control Run BLAST with Run BLAST with HEK293T cells transfected with pcmv6-entry AFAP. Human breast adenocarcinoma, colon, kidney carcinoma, ovary adenocarcinoma, endometrium, prostate and prostate carcinoma tissue. Properties Form Storage instructions Liquid Shipped at 4 C. Store at +4 C short term (1-2 weeks). Upon delivery aliquot. Store at -20 C long term. Avoid freeze / thaw cycle. Storage buffer ph: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 1% BSA, 48.08% PBS Purity Clonality Protein G purified Monoclonal 1

2 Clone number Isotype 2G9 IgG1 Applications Our Abpromise guarantee covers the use of ab in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes IHC-P 1/150. WB 1/2000. Target Function Tissue specificity Sequence similarities Post-translational modifications Cellular localization Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma. Contains 2 PH domains. Phosphorylated on tyrosine residues by SRC. Cytoplasm > cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction. Anti-AFAP antibody [2G9] images fixed/ paraffin embedded Human prostate carcinoma tissue labeling AFAP with 2

3 fixed/ paraffin embedded Human prostate tissue labeling AFAP with ab at a 1/150 dilution. fixed/ paraffin embedded Human endometrium tissue labeling AFAP with fixed/ paraffin embedded Human ovary adenocarcinoma tissue labeling AFAP with fixed/ paraffin embedded Human kidney carcinoma tissue labeling AFAP with 3

4 fixed/ paraffin embedded Human colon tissue labeling AFAP with ab at a 1/150 dilution. fixed/ paraffin embedded Human breast adenocarcinoma tissue labeling AFAP with All lanes : Anti-AFAP antibody [2G9] at 1/2000 dilution Lane 1 : HEK293T cells were transfected with the pcmv6-entry control Lane 2 : HEK293T cells transfected with the pcmv6-entry AFAP Western blot - Anti-AFAP [2G9] antibody Lysates/proteins at 5 µg per lane. HEK293T cell lysates were generated from transient transfection of the cdna clone (RC218730) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards 4

5 If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 5