Microarray technology
|
|
|
- May Rich
- 7 years ago
- Views:
Transcription
1 07 Microarray Microarray technology Data acquisition Image processing Data analysis alessandro bogliolo isti information science and technology institute 1/17 Microarray technology Array Type Spot Density (per cm 2 ) Probe Target Labeling Nylon Macroarrays < 100 cdna RNA Radio Nylon Microarrays < 5000 cdna mrna Radio/Flour Glass Microarrays < 10,000 cdna mrna Fluor Oligonucleotide Chips <250,000 oligo's mrna Fluor alessandro bogliolo isti information science and technology institute 2/17
2 Microarray experiment 30min 1hr 2hr 4hr 30min 2MA 1hr 2MA 2hr 2MA 4hr 2MA 30min AIG 1hr AIG 2hr AIG 4hr AIG. 132 conditions Gene 1 Gene 2 Gene 3 Gene genes alessandro bogliolo isti information science and technology institute 3/17 Microarray scanner alessandro bogliolo isti information science and technology institute 4/17
3 Microarray scanner alessandro bogliolo isti information science and technology institute 5/17 Noise Noise sources: Sample preparation, labeling, amplification Reaction variations Environment Target volume Hybridization parameters (temperature, time,...) Aspecific hybridization Dust Scanner settings Quantization alessandro bogliolo isti information science and technology institute 6/17
4 Noise filtering (1) Gridding: identify spot locations Segmentation: distinguish foreground from background Fixed Circle: put a circle around the foreground area Seeded region growing: identify initial spot seeds and grow high intensity regions Edge detection algorithms Background cancellation Intensity = FGintensity - BGintensity alessandro bogliolo isti information science and technology institute 7/17 Noise filtering (2) alessandro bogliolo isti information science and technology institute 8/17
5 Quality evaluation Irregular size or shape Irregular placement Low intensity Saturation Spot variance Background variance indistinguishable bad print saturated misalignment artifact alessandro bogliolo isti information science and technology institute 9/17 Normalization (1) Normalize data to correct for artificial variances Red = FGred - BGred Green = FGgreen BGgreen PixelValue = log 2 (Red/Green)- log 2 (Red avg /Green avg ) Pixel color: Green if pixel value < 0 Yellow if pixel value = 0 Red if pixel value > 0 alessandro bogliolo isti information science and technology institute 10/17
6 Normalization (2) Uncalibrated, red light under detected Calibrated, red and green equally detected alessandro bogliolo isti information science and technology institute 11/17 Data analysis: Scatterplot alessandro bogliolo isti information science and technology institute 12/17
7 Data analysis: Classification Goal: Identify subset of genes that distinguish between treatments, tissues, etc. Method Collect several samples grouped by treatments (e.g. ALL vs. AML) Use genes as features Build a classifier to distinguish treatments Classifiers Neural networks, decision trees,... alessandro bogliolo isti information science and technology institute 13/17 Principal component analysis alessandro bogliolo isti information science and technology institute 14/17
8 Clustering Hypothesis: Genes with similar function have similar expression profiles Find groups of genes with similar expression profiles Find groups of individuals with similar expression profiles within a population alessandro bogliolo isti information science and technology institute 15/17 Clustering: k-mean algorithm A. B. C. D. alessandro bogliolo isti information science and technology institute 16/17
9 Hyerarchical clustering Top-down (division) Bottom-up (agglomeration) alessandro bogliolo isti information science and technology institute 17/17
Basic Analysis of Microarray Data
Basic Analysis of Microarray Data A User Guide and Tutorial Scott A. Ness, Ph.D. Co-Director, Keck-UNM Genomics Resource and Dept. of Molecular Genetics and Microbiology University of New Mexico HSC Tel.
Molecular Genetics: Challenges for Statistical Practice. J.K. Lindsey
Molecular Genetics: Challenges for Statistical Practice J.K. Lindsey 1. What is a Microarray? 2. Design Questions 3. Modelling Questions 4. Longitudinal Data 5. Conclusions 1. What is a microarray? A microarray
Analysis of gene expression data. Ulf Leser and Philippe Thomas
Analysis of gene expression data Ulf Leser and Philippe Thomas This Lecture Protein synthesis Microarray Idea Technologies Applications Problems Quality control Normalization Analysis next week! Ulf Leser:
Microarray Technology
Microarrays And Functional Genomics CPSC265 Matt Hudson Microarray Technology Relatively young technology Usually used like a Northern blot can determine the amount of mrna for a particular gene Except
How many of you have checked out the web site on protein-dna interactions?
How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss
Measuring gene expression (Microarrays) Ulf Leser
Measuring gene expression (Microarrays) Ulf Leser This Lecture Gene expression Microarrays Idea Technologies Problems Quality control Normalization Analysis next week! 2 http://learn.genetics.utah.edu/content/molecules/transcribe/
Data Mining Cluster Analysis: Basic Concepts and Algorithms. Lecture Notes for Chapter 8. Introduction to Data Mining
Data Mining Cluster Analysis: Basic Concepts and Algorithms Lecture Notes for Chapter 8 by Tan, Steinbach, Kumar 1 What is Cluster Analysis? Finding groups of objects such that the objects in a group will
Environmental Remote Sensing GEOG 2021
Environmental Remote Sensing GEOG 2021 Lecture 4 Image classification 2 Purpose categorising data data abstraction / simplification data interpretation mapping for land cover mapping use land cover class
Perception of Light and Color
Perception of Light and Color Theory and Practice Trichromacy Three cones types in retina a b G+B +R Cone sensitivity functions 100 80 60 40 20 400 500 600 700 Wavelength (nm) Short wavelength sensitive
REAL TIME PCR USING SYBR GREEN
REAL TIME PCR USING SYBR GREEN 1 THE PROBLEM NEED TO QUANTITATE DIFFERENCES IN mrna EXPRESSION SMALL AMOUNTS OF mrna LASER CAPTURE SMALL AMOUNTS OF TISSUE PRIMARY CELLS PRECIOUS REAGENTS 2 THE PROBLEM
MultiExperiment Viewer Quickstart Guide
MultiExperiment Viewer Quickstart Guide Table of Contents: I. Preface - 2 II. Installing MeV - 2 III. Opening a Data Set - 2 IV. Filtering - 6 V. Clustering a. HCL - 8 b. K-means - 11 VI. Modules a. T-test
Comparison of Non-linear Dimensionality Reduction Techniques for Classification with Gene Expression Microarray Data
CMPE 59H Comparison of Non-linear Dimensionality Reduction Techniques for Classification with Gene Expression Microarray Data Term Project Report Fatma Güney, Kübra Kalkan 1/15/2013 Keywords: Non-linear
Image Analysis Using the Aperio ScanScope
Image Analysis Using the Aperio ScanScope Allen H. Olson, PhD Algorithm Development Engineer Aperio Technologies INTRODUCTION Why should I choose the Aperio ScanScope over competing systems for image analysis?
K-Type Thermocouple Sensor User s Guide
K-Type Thermocouple Sensor User s Guide 1 TABLE OF CONTENTS: 1 INTRODUCTION... 2 2 TYPICAL APPLICATION:... 2 3 INSTALLATION RULES:... 2 3.1 Connecting the sensor to M1/MD4 data logger:... 2 3.2 Connecting
Data Mining. 1 Introduction 2 Data Mining methods. Alfred Holl Data Mining 1
Data Mining 1 Introduction 2 Data Mining methods Alfred Holl Data Mining 1 1 Introduction 1.1 Motivation 1.2 Goals and problems 1.3 Definitions 1.4 Roots 1.5 Data Mining process 1.6 Epistemological constraints
Technical Considerations Detecting Transparent Materials in Particle Analysis. Michael Horgan
Technical Considerations Detecting Transparent Materials in Particle Analysis Michael Horgan Who We Are Fluid Imaging Technology Manufacturers of the FlowCam series of particle analyzers FlowCam HQ location
Foundations of Artificial Intelligence. Introduction to Data Mining
Foundations of Artificial Intelligence Introduction to Data Mining Objectives Data Mining Introduce a range of data mining techniques used in AI systems including : Neural networks Decision trees Present
Valutazione di software di analisi di microarray basato su simulazioni di immagini da dati reali
Testing and evaluation of microarray image analysis software Valutazione di software di analisi di microarray basato su simulazioni di immagini da dati reali Ignazio Infantino Tutorial su Metodi e strumenti
Capturing Best Practice for Microarray Gene Expression Data Analysis
Capturing Best Practice for Microarray Gene Expression Data Analysis Gregory Piatetsky-Shapiro KDnuggets gps@kdnuggets.com Tom Khabaza SPSS tkhabaza@spss.com Sridhar Ramaswamy MIT / Whitehead Institute
Introduction to Pattern Recognition
Introduction to Pattern Recognition Selim Aksoy Department of Computer Engineering Bilkent University saksoy@cs.bilkent.edu.tr CS 551, Spring 2009 CS 551, Spring 2009 c 2009, Selim Aksoy (Bilkent University)
SmartFlare RNA Detection Probes: Principles, protocols and troubleshooting
Technical Guide SmartFlare RNA Detection Probes: Principles, protocols and troubleshooting Principles of SmartFlare technology RNA detection traditionally requires transfection, laborious sample prep,
Statistical Issues in cdna Microarray Data Analysis
Citation: Smyth, G. K., Yang, Y.-H., Speed, T. P. (2003). Statistical issues in cdna microarray data analysis. Methods in Molecular Biology 224, 111-136. [PubMed ID 12710670] Statistical Issues in cdna
Data Mining and Visualization
Data Mining and Visualization Jeremy Walton NAG Ltd, Oxford Overview Data mining components Functionality Example application Quality control Visualization Use of 3D Example application Market research
Ensemble Methods. Knowledge Discovery and Data Mining 2 (VU) (707.004) Roman Kern. KTI, TU Graz 2015-03-05
Ensemble Methods Knowledge Discovery and Data Mining 2 (VU) (707004) Roman Kern KTI, TU Graz 2015-03-05 Roman Kern (KTI, TU Graz) Ensemble Methods 2015-03-05 1 / 38 Outline 1 Introduction 2 Classification
T O B C A T C A S E G E O V I S A T DETECTIE E N B L U R R I N G V A N P E R S O N E N IN P A N O R A MISCHE BEELDEN
T O B C A T C A S E G E O V I S A T DETECTIE E N B L U R R I N G V A N P E R S O N E N IN P A N O R A MISCHE BEELDEN Goal is to process 360 degree images and detect two object categories 1. Pedestrians,
Data Acquisition. DNA microarrays. The functional genomics pipeline. Experimental design affects outcome data analysis
Data Acquisition DNA microarrays The functional genomics pipeline Experimental design affects outcome data analysis Data acquisition microarray processing Data preprocessing scaling/normalization/filtering
Gene expression analysis. Ulf Leser and Karin Zimmermann
Gene expression analysis Ulf Leser and Karin Zimmermann Ulf Leser: Bioinformatics, Wintersemester 2010/2011 1 Last lecture What are microarrays? - Biomolecular devices measuring the transcriptome of a
Oligonucleotide Stability Study
Oligonucleotide Stability Study IDT is performing a long term study of oligo stability. The study has been ongoing for 2 years and has tested oligo stability under various conditions. An overview of the
Learning Example. Machine learning and our focus. Another Example. An example: data (loan application) The data and the goal
Learning Example Chapter 18: Learning from Examples 22c:145 An emergency room in a hospital measures 17 variables (e.g., blood pressure, age, etc) of newly admitted patients. A decision is needed: whether
Real-time qpcr Assay Design Software www.qpcrdesign.com
Real-time qpcr Assay Design Software www.qpcrdesign.com Your Blueprint For Success Informational Guide 2199 South McDowell Blvd Petaluma, CA 94954-6904 USA 1.800.GENOME.1(436.6631) 1.415.883.8400 1.415.883.8488
SICKLE CELL ANEMIA & THE HEMOGLOBIN GENE TEACHER S GUIDE
AP Biology Date SICKLE CELL ANEMIA & THE HEMOGLOBIN GENE TEACHER S GUIDE LEARNING OBJECTIVES Students will gain an appreciation of the physical effects of sickle cell anemia, its prevalence in the population,
Microarray Data Analysis. A step by step analysis using BRB-Array Tools
Microarray Data Analysis A step by step analysis using BRB-Array Tools 1 EXAMINATION OF DIFFERENTIAL GENE EXPRESSION (1) Objective: to find genes whose expression is changed before and after chemotherapy.
QPCR Applications using Stratagene s Mx Real-Time PCR Platform
QPCR Applications using Stratagene s Mx Real-Time PCR Platform Dan Schoeffner, Ph.D Field Applications Scientist Dan.Schoeffner@Stratagene.com Tech. Services 800-894-1304 Polymerase Chain Reaction Melt
DATA MINING CLUSTER ANALYSIS: BASIC CONCEPTS
DATA MINING CLUSTER ANALYSIS: BASIC CONCEPTS 1 AND ALGORITHMS Chiara Renso KDD-LAB ISTI- CNR, Pisa, Italy WHAT IS CLUSTER ANALYSIS? Finding groups of objects such that the objects in a group will be similar
Automatic Labeling of Lane Markings for Autonomous Vehicles
Automatic Labeling of Lane Markings for Autonomous Vehicles Jeffrey Kiske Stanford University 450 Serra Mall, Stanford, CA 94305 jkiske@stanford.edu 1. Introduction As autonomous vehicles become more popular,
Machine vision systems - 2
Machine vision systems Problem definition Image acquisition Image segmentation Connected component analysis Machine vision systems - 1 Problem definition Design a vision system to see a flat world Page
APC APPLICATION NOTE #92
#92 Best Practices for Designing Data Centers with the InfraStruXure InRow RC By John Niemann Abstract The InfraStruXure InRow RC is designed to provide cooling at the row and rack level of a data center
Machine Learning: Overview
Machine Learning: Overview Why Learning? Learning is a core of property of being intelligent. Hence Machine learning is a core subarea of Artificial Intelligence. There is a need for programs to behave
Core Facility Genomics
Core Facility Genomics versatile genome or transcriptome analyses based on quantifiable highthroughput data ascertainment 1 Topics Collaboration with Harald Binder and Clemens Kreutz Project: Microarray
Long-Term Effects of Drug Addiction
Long-Term Effects of Drug Addiction Part 1: Addiction is a chronic disease Drug addiction is considered a chronic brain disease because drugs cause long-lasting changes in brain structure and function.
Patient Booking Website
Patient Booking Website This presentation includes the following topics. Please select a section below to continue. Key feature of the patient booking website Logging in Booking an appointment Changing
Products - Microarray Scanners - SpotLight Two-Color Microarray Fluorescence Scanners New! SpotLight 2 now available!
Products - Microarray Scanners - SpotLight Two-Color Microarray Fluorescence Scanners New! SpotLight 2 now available! Arrayit SpotLight Two-Color Microarray Scanners provide the market s most affordable
OUTLIER ANALYSIS. Data Mining 1
OUTLIER ANALYSIS Data Mining 1 What Are Outliers? Outlier: A data object that deviates significantly from the normal objects as if it were generated by a different mechanism Ex.: Unusual credit card purchase,
Exiqon Array Software Manual. Quick guide to data extraction from mircury LNA microrna Arrays
Exiqon Array Software Manual Quick guide to data extraction from mircury LNA microrna Arrays March 2010 Table of contents Introduction Overview...................................................... 3 ImaGene
Cluster Analysis: Basic Concepts and Algorithms
Cluster Analsis: Basic Concepts and Algorithms What does it mean clustering? Applications Tpes of clustering K-means Intuition Algorithm Choosing initial centroids Bisecting K-means Post-processing Strengths
Tutorial for Tracker and Supporting Software By David Chandler
Tutorial for Tracker and Supporting Software By David Chandler I use a number of free, open source programs to do video analysis. 1. Avidemux, to exerpt the video clip, read the video properties, and save
Correcting the Lateral Response Artifact in Radiochromic Film Images from Flatbed Scanners
Correcting the Lateral Response Artifact in Radiochromic Film Images from Flatbed Scanners Background The lateral response artifact (LRA) in radiochromic film images from flatbed scanners was first pointed
Assessment. Presenter: Yupu Zhang, Guoliang Jin, Tuo Wang Computer Vision 2008 Fall
Automatic Photo Quality Assessment Presenter: Yupu Zhang, Guoliang Jin, Tuo Wang Computer Vision 2008 Fall Estimating i the photorealism of images: Distinguishing i i paintings from photographs h Florin
Fluorescent dyes for use with the
Detection of Multiple Reporter Dyes in Real-time, On-line PCR Analysis with the LightCycler System Gregor Sagner, Cornelia Goldstein, and Rob van Miltenburg Roche Molecular Biochemicals, Penzberg, Germany
Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS)
Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS) A typical RNA Seq experiment Library construction Protocol variations Fragmentation methods RNA: nebulization,
COLOR THEORY WORKSHEET
COLOR THEORY WORKSHEET Use color pencils to complete the following exercises Name: Period Date PRIMARY COLORS cannot be made from any combination of colors. Fade intensity from top left to bottom right
Supervised and unsupervised learning - 1
Chapter 3 Supervised and unsupervised learning - 1 3.1 Introduction The science of learning plays a key role in the field of statistics, data mining, artificial intelligence, intersecting with areas in
ENVI Classic Tutorial: Atmospherically Correcting Multispectral Data Using FLAASH 2
ENVI Classic Tutorial: Atmospherically Correcting Multispectral Data Using FLAASH Atmospherically Correcting Multispectral Data Using FLAASH 2 Files Used in this Tutorial 2 Opening the Raw Landsat Image
Lecture 2: Descriptive Statistics and Exploratory Data Analysis
Lecture 2: Descriptive Statistics and Exploratory Data Analysis Further Thoughts on Experimental Design 16 Individuals (8 each from two populations) with replicates Pop 1 Pop 2 Randomly sample 4 individuals
Technical Note. Roche Applied Science. No. LC 19/2004. Color Compensation
Roche Applied Science Technical Note No. LC 19/2004 Purpose of this Note Color The LightCycler System is able to simultaneously detect and analyze more than one color in each capillary. Due to overlap
A Process Flow for Classification and Clustering of Fruit Fly Gene Expression Patterns
A Process Flow for Classification and Clustering of Fruit Fly Gene Expression Patterns Andreas Heffel, Peter F. Stadler, Sonja J. Prohaska, Gerhard Kauer, Jens-Peer Kuska Santa Fe Institute Department
UNSUPERVISED MACHINE LEARNING TECHNIQUES IN GENOMICS
UNSUPERVISED MACHINE LEARNING TECHNIQUES IN GENOMICS Dwijesh C. Mishra I.A.S.R.I., Library Avenue, New Delhi-110 012 dcmishra@iasri.res.in What is Learning? "Learning denotes changes in a system that enable
EFFICIENT VEHICLE TRACKING AND CLASSIFICATION FOR AN AUTOMATED TRAFFIC SURVEILLANCE SYSTEM
EFFICIENT VEHICLE TRACKING AND CLASSIFICATION FOR AN AUTOMATED TRAFFIC SURVEILLANCE SYSTEM Amol Ambardekar, Mircea Nicolescu, and George Bebis Department of Computer Science and Engineering University
12-Steps: Searching & Registering for Classes
Office of the Registrar. Division of Student Affairs 12-Steps: Searching & Registering for Classes Step 1: Access your web browser and log on to www.ucdenver.edu/ucdaccess. Proceed to login with your User
Speed Performance Improvement of Vehicle Blob Tracking System
Speed Performance Improvement of Vehicle Blob Tracking System Sung Chun Lee and Ram Nevatia University of Southern California, Los Angeles, CA 90089, USA sungchun@usc.edu, nevatia@usc.edu Abstract. A speed
GSR Microarrays Project Management System
GSR Microarrays Project Management System A User s Guide GSR Microarrays Vanderbilt University MRBIII, Room 9274 465 21 st Avenue South Nashville, TN 37232 microarray@vanderbilt.edu (615) 936-3003 www.gsr.vanderbilt.edu
Microarray Data Analysis Workshop. Custom arrays and Probe design Probe design in a pangenomic world. Carsten Friis. MedVetNet Workshop, DTU 2008
Microarray Data Analysis Workshop MedVetNet Workshop, DTU 2008 Custom arrays and Probe design Probe design in a pangenomic world Carsten Friis Media glna tnra GlnA TnrA C2 glnr C3 C5 C6 K GlnR C1 C4 C7
issues Scanning Technologies Technology Cost Scanning Productivity Geometric Scanning Accuracy Total Cost of Ownership (TCO) Scanning Reliability
CISvs.CCD Scanning Technologies issues to consider before purchasing a wide format scanner Technology Cost Scanning Productivity Geometric Scanning Accuracy Total Cost of Ownership (TCO) Scanning Reliability
Best Practices for Dashboard Design with SAP BusinessObjects Design Studio
Ingo Hilgefort, SAP Mentor February 2015 Agenda Best Practices on Dashboard Design Performance BEST PRACTICES FOR DASHBOARD DESIGN WITH SAP BUSINESSOBJECTS DESIGN STUDIO DASHBOARD DESIGN What is a dashboard
Norbert Schuff Professor of Radiology VA Medical Center and UCSF Norbert.schuff@ucsf.edu
Norbert Schuff Professor of Radiology Medical Center and UCSF Norbert.schuff@ucsf.edu Medical Imaging Informatics 2012, N.Schuff Course # 170.03 Slide 1/67 Overview Definitions Role of Segmentation Segmentation
K-Means Cluster Analysis. Tan,Steinbach, Kumar Introduction to Data Mining 4/18/2004 1
K-Means Cluster Analsis Chapter 3 PPDM Class Tan,Steinbach, Kumar Introduction to Data Mining 4/18/4 1 What is Cluster Analsis? Finding groups of objects such that the objects in a group will be similar
Data Mining. Nonlinear Classification
Data Mining Unit # 6 Sajjad Haider Fall 2014 1 Nonlinear Classification Classes may not be separable by a linear boundary Suppose we randomly generate a data set as follows: X has range between 0 to 15
Introduction to Machine Learning and Data Mining. Prof. Dr. Igor Trajkovski trajkovski@nyus.edu.mk
Introduction to Machine Learning and Data Mining Prof. Dr. Igor Trajkovski trajkovski@nyus.edu.mk Ensembles 2 Learning Ensembles Learn multiple alternative definitions of a concept using different training
ALLEN Mouse Brain Atlas
TECHNICAL WHITE PAPER: QUALITY CONTROL STANDARDS FOR HIGH-THROUGHPUT RNA IN SITU HYBRIDIZATION DATA GENERATION Consistent data quality and internal reproducibility are critical concerns for high-throughput
Step-by-Step Guide to Basic Expression Analysis and Normalization
Step-by-Step Guide to Basic Expression Analysis and Normalization Page 1 Introduction This document shows you how to perform a basic analysis and normalization of your data. A full review of this document
Application Note. Single Cell PCR Preparation
Application Note Single Cell PCR Preparation From Automated Screening to the Molecular Analysis of Single Cells The AmpliGrid system is a highly sensitive tool for the analysis of single cells. In combination
Java Modules for Time Series Analysis
Java Modules for Time Series Analysis Agenda Clustering Non-normal distributions Multifactor modeling Implied ratings Time series prediction 1. Clustering + Cluster 1 Synthetic Clustering + Time series
Hierarchical Clustering Analysis
Hierarchical Clustering Analysis What is Hierarchical Clustering? Hierarchical clustering is used to group similar objects into clusters. In the beginning, each row and/or column is considered a cluster.
Cluster software and Java TreeView
Cluster software and Java TreeView To download the software: http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm http://bonsai.hgc.jp/~mdehoon/software/cluster/manual/treeview.html Cluster 3.0
I. Create the base view with the data you want to measure
Developing Key Performance Indicators (KPIs) in Tableau The following tutorial will show you how to create KPIs in Tableau 9. To get started, you will need the following: Tableau version 9 Data: Sample
WebFOCUS RStat. RStat. Predict the Future and Make Effective Decisions Today. WebFOCUS RStat
Information Builders enables agile information solutions with business intelligence (BI) and integration technologies. WebFOCUS the most widely utilized business intelligence platform connects to any enterprise
How To Cluster
Data Clustering Dec 2nd, 2013 Kyrylo Bessonov Talk outline Introduction to clustering Types of clustering Supervised Unsupervised Similarity measures Main clustering algorithms k-means Hierarchical Main
Using Image J to Measure the Brightness of Stars (Written by Do H. Kim)
Using Image J to Measure the Brightness of Stars (Written by Do H. Kim) What is Image J? Image J is Java-based image processing program developed at the National Institutes of Health. Image J runs on everywhere,
Calibrating your monitor
Tutorial Calibrating your monitor Software version: Asanti 2.0 Document version: June 23, 2015 This tutorial demonstrates how to calibrate your monitor with ColorTune Display for color accuracy. 1. Install
Digital Image Increase
Exploiting redundancy for reliable aerial computer vision 1 Digital Image Increase 2 Images Worldwide 3 Terrestrial Image Acquisition 4 Aerial Photogrammetry 5 New Sensor Platforms Towards Fully Automatic
Supervised Classification workflow in ENVI 4.8 using WorldView-2 imagery
Supervised Classification workflow in ENVI 4.8 using WorldView-2 imagery WorldView-2 is the first commercial high-resolution satellite to provide eight spectral sensors in the visible to near-infrared
CPI Links Content Guide & Five Items Resource
CPI Links Content Guide & Five Items Resource Introduction The following information should be used as a companion to the CPI Links. It provides clarifications concerning the content and skills contained
Machine Learning for Medical Image Analysis. A. Criminisi & the InnerEye team @ MSRC
Machine Learning for Medical Image Analysis A. Criminisi & the InnerEye team @ MSRC Medical image analysis the goal Automatic, semantic analysis and quantification of what observed in medical scans Brain
CompleteⅡ 1st strand cdna Synthesis Kit
Instruction Manual CompleteⅡ 1st strand cdna Synthesis Kit Catalog # GM30401, GM30402 Green Mountain Biosystems. LLC Web: www.greenmountainbio.com Tel: 800-942-1160 Sales: Sales@ greenmountainbio.com Support:
Computer Vision for Quality Control in Latin American Food Industry, A Case Study
Computer Vision for Quality Control in Latin American Food Industry, A Case Study J.M. Aguilera A1, A. Cipriano A1, M. Eraña A2, I. Lillo A1, D. Mery A1, and A. Soto A1 e-mail: [jmaguile,aciprian,dmery,asoto,]@ing.puc.cl
Teacher Management. Estimated Time for Completion 2 class periods
AP Biology, Aquatic Science, Environmental Systems Morphometric Analysis of Eurycea in Austin, TX Teacher Page Essential Questions: Enduring Understandings Nature of Science and Data Vocabulary Morphometric
Multiscale Object-Based Classification of Satellite Images Merging Multispectral Information with Panchromatic Textural Features
Remote Sensing and Geoinformation Lena Halounová, Editor not only for Scientific Cooperation EARSeL, 2011 Multiscale Object-Based Classification of Satellite Images Merging Multispectral Information with
Introduction to Artificial Intelligence G51IAI. An Introduction to Data Mining
Introduction to Artificial Intelligence G51IAI An Introduction to Data Mining Learning Objectives Introduce a range of data mining techniques used in AI systems including : Neural networks Decision trees
Analysis of Illumina Gene Expression Microarray Data
Analysis of Illumina Gene Expression Microarray Data Asta Laiho, Msc. Tech. Bioinformatics research engineer The Finnish DNA Microarray Centre Turku Centre for Biotechnology, Finland The Finnish DNA Microarray
What is it? Dichotomous Keys Teacher Information
STO-110 What is it? Dichotomous Keys Teacher Information Summary Students use a dichotomous key to identify a collection of plastic frogs. They design and test a dichotomous key that could be used to identify
Real time and Quantitative (RTAQ) PCR. so I have an outlier and I want to see if it really is changed
Real time and Quantitative (RTAQ) PCR or.. for this audience so I have an outlier and I want to see if it really is changed Nigel Walker, Ph.D. Laboratory of Computational Biology and Risk Analysis, Environmental
JACIE Science Applications of High Resolution Imagery at the USGS EROS Data Center
JACIE Science Applications of High Resolution Imagery at the USGS EROS Data Center November 8-10, 2004 U.S. Department of the Interior U.S. Geological Survey Michael Coan, SAIC USGS EROS Data Center coan@usgs.gov
Flower Model: Teacher Instructions Sepals Anther Stamens (male) Filament Stigma Pistil Style (female) Ovary Petals sepals petals stamens pistil
Flower Model: Teacher Instructions In order to better understand the reproductive cycle of a flower, take a look at some flowers and note the male and female parts. Most flowers are different; some have
Correlation of microarray and quantitative real-time PCR results. Elisa Wurmbach Mount Sinai School of Medicine New York
Correlation of microarray and quantitative real-time PCR results Elisa Wurmbach Mount Sinai School of Medicine New York Microarray techniques Oligo-array: Affymetrix, Codelink, spotted oligo-arrays (60-70mers)
Cluster Analysis. Alison Merikangas Data Analysis Seminar 18 November 2009
Cluster Analysis Alison Merikangas Data Analysis Seminar 18 November 2009 Overview What is cluster analysis? Types of cluster Distance functions Clustering methods Agglomerative K-means Density-based Interpretation
Scheduling a New Student Conference date: 1. Log into myuh (http://my.uh.edu) 2. Click the UH Self-Service link.
Scheduling a New Student Conference date: 1. Log into myuh (http://my.uh.edu) 2. Click the UH Self-Service link. 3. Click the Student Administration link in the lefthand menu 5. The New Student Conference
Final Project Report
CPSC545 by Introduction to Data Mining Prof. Martin Schultz & Prof. Mark Gerstein Student Name: Yu Kor Hugo Lam Student ID : 904907866 Due Date : May 7, 2007 Introduction Final Project Report Pseudogenes
White paper. CCD and CMOS sensor technology Technical white paper
White paper CCD and CMOS sensor technology Technical white paper Table of contents 1. Introduction to image sensors 3 2. CCD technology 4 3. CMOS technology 5 4. HDTV and megapixel sensors 6 5. Main differences
Recombinant DNA and Biotechnology
Recombinant DNA and Biotechnology Chapter 18 Lecture Objectives What Is Recombinant DNA? How Are New Genes Inserted into Cells? What Sources of DNA Are Used in Cloning? What Other Tools Are Used to Study
Essentials of Real Time PCR. About Sequence Detection Chemistries
Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected