Post genomics techniques for improved understanding of bioprocesses. Caroline Evans, University of Sheffield
|
|
- Kellie Barnett
- 7 years ago
- Views:
Transcription
1 Post genomics techniques for improved understanding of bioprocesses Caroline Evans, University of Sheffield
2 Life Science Interface OMIC focussed research
3 Talk Overview Post genomic view applications of Proteomics Engineering strategies - Use of E coli as a platform for bioprocessing 1. Improving glycosylation efficiency 2. Mass spectrometry methods for glycosylation analysis New Advanced Biomanufacturing Centre University of Sheffield
4 Post genomics deals with complexity beyond the genome Genome Gene Transcription Transcriptome mrna (s) Splice variants Translation Proteome Protein (s) Multiple isoforms possible Proteome - Functional regulation Dynamic Metabolites post-translational modification, proteoforms altered protein-protein interactions protein turnover subcellular localisation Complexity
5 Proteomics Provides a Toolkit for bioprocess optimisation Proteins and peptides can be characterised using a combination of separation and characterisation High Performance Liquid Chromatography (HPLC) Typically peptide separation (shot gun, bottom up analysis) Mass spectrometry (MS) Measures molecular weight (as mass/charge)
6 Applications of Mass Spectrometry Protein identification based on mass measurements of peptides, peptide fragments (infer sequence) Perform quantification: Relative and Absolute Protein characterisation -Analysis of co and posttranslational modifications (PTMs), status and site localisation
7 Glycoprotein characterisation Dr Jags Pandhal, Dr Caroline Evans, Prof Phil Wright Glycoprotein and glycopeptides can be characterised using a combination of High Performance Liquid Chromatography (HPLC) separation of glycosylated and non glycosylated isoforms Mass spectrometry (MS) identification of glycopeptides, quantification
8 Why focus on Glycosylation? An enzymatic process that attaches glycans to proteins Biological Significance?: Improve pharmacokinetic properties Half life Solubility Stability 70% industrially relevant proteins are glycosylated
9 Types of Glycosylation N + O-Linked, CHO is the industrial platform for eukaryotic glycoprotein production Bacterial systems? Discovery of C. jejuni N glycosylation pathway. Functional on transfer into E. coli Industrial biotech application CHO alternative Benefits include cost reduction, faster time scale and reduction variable glycosylation specificity observed with CHO cells.
10 Engineering Approaches use of model system N-Glycosylation machinery successfully transferred into E. coli from C. jejuni Opens up the possibility to produce recombinant glycoproteins in the bacterial workhorse AcrA model protein for initial work
11 What can we do now? Prokaryotic Eukaryotic Eukaryotic type glycan desirable as reduce immunogenic response.
12 E. coli can glycosylate proteins The efficiency of the process is poor low yields, high metabolic burden Diversify the functionality of E. coli as a protein production host How? Improve glycosylation process efficiency By? Developing and implementing omics tools
13 Forward Engineering I Approach: Mass spectrometry (itraq) based protein profiling of E.coli cells expressing and glycosylating recombinant AcrA protein combined with probability based analysis of metabolic changes. Isocitrate lyase (ICL) key regulatory enzyme of glyoxylate cycle E. coli was forward engineered to increase expression of ICL enhance the flux through the glyoxylate cycle, increasing the amount of glycosylated AcrA by 300%. Pandhal, J, Ow SY, Noirel, J and Wright PC (2011) Biotechnology and Bioengineering, 108: ,
14 Forward Engineering II Using western blots and pseudo selective reactive monitoring to analyse glycosylation, codon optimisation of the bacterial oligosaccharyltransferase, PglB, when expressed in E. coli, improved AcrA glycosylation efficiency by approx 2 fold** Pandhal J, Desai P, Walpole C, Doroudi L, Malyshev D, Wright PC Biochemical and Biophysical Research Communication 419:472-6.
15 ** Improving the tool kit - Quantification Schiff s reagent Qualitative Western blots Semiquantitative Selective reaction monitoring (mass spectrometry) Quantitative Pandhal J, Desai P, Walpole C, Doroudi L, Malyshev D, Wright PC Biochemical and Biophysical Research Communication 419:472-6.
16 yield coefficient (mg/g) Inverse metabolic engineering (codon optimisation) Increase base line level protein production Different E.coli strains: CLM24 Remove lipid anchor (PelB signal sequence retained) = soluble periplasmic glycoprotein Changed expression vector: arabinose inducible (dose dependent) fold increase E.coli BL21 pet21a+ Soluble AcrA E.coli CLM24, pecacra Base line level: ca. 1.5 mg/l gacra Pandhal J, Woodruff LB, Jaffe S, Desai P, Ow SY, Noirel J, Gill RT, Wright PC (2013) Biotechnol Bioeng 110:
17 Novel MS Methodology for Glycopeptide Analysis Key requirements: Screen for glycopeptides Makes no assumption about precursor m/z Provides information for targeted analysis complement SRM/MRM Key Features: Monitor glycosylation diagnostic ions in relation to precursor MS High resolution MS for confidence in diagnostic ion assignment 17
18 Intensity Intensity Collision Induced Release of Glyco Diagnostic m/z , m/z diagnostic ions Precursor ions HexNAc and Hex-HexNAc diagnostic Alignment Precursor and Fragment ion MS survey scan (10 ev) m/z Alternating bbcid MS/MS scan (25-75 ev) m/z Caroline Evans, Narciso Couto, Liliya Davlyatova, Phil Wright (manuscript in preparation) 18
19 Summary - Metabolic engineering using proteomics tools
20 A New Interdisciplinary Centre for Biomanufacturing Research Advanced Biomanufacturing Centre Engineering Science Medicine Automatic and Control Systems Materials Kroto Institute Biomedical Molecular Biology Animal and Plant Sciences Biomedical Science Chemistry Computer Science sitran Medical School Dentistry Director: Prof David James
21 Bioindustrial Limitations on New Technology Development Industrial Drivers for Cost-Effective Academic Collaboration: Transformation/Disruption Translation Information Innovation
22 Right First Time Engineering Design for Predictable Bioprocesses Contact us:
23 Advanced Bio Manufacturing Centre (ABC) Bioproduct and Technology Sectors Bioproducts Industrial chemicals Synthetic biomolecules Biopharmaceuticals Cell and tissue therapies Biomaterials Technologies Process Engineering Synthetic Biology Computational Biology Bioanalysis Cell and molecular biology Systems biology and omics
24 Acknowledgements Professor Phillip Wright Professor David James Dr Jags Pandhal Dr. S. Ow Dr. J. Noirel Dr Narciso Couto
Introduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Tim Griffin Associate Professor, BMBB Faculty Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s
More informationBBSRC TECHNOLOGY STRATEGY: TECHNOLOGIES NEEDED BY RESEARCH KNOWLEDGE PROVIDERS
BBSRC TECHNOLOGY STRATEGY: TECHNOLOGIES NEEDED BY RESEARCH KNOWLEDGE PROVIDERS 1. The Technology Strategy sets out six areas where technological developments are required to push the frontiers of knowledge
More informationQuantitative proteomics background
Proteomics data analysis seminar Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post transcriptional regulation of key cellular processes de Groot, M., Daran
More informationDr Alexander Henzing
Horizon 2020 Health, Demographic Change & Wellbeing EU funding, research and collaboration opportunities for 2016/17 Innovate UK funding opportunities in omics, bridging health and life sciences Dr Alexander
More informationRetrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem
Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Kevin Van Cott, Associate Professor Dept. of Chemical and Biomolecular Engineering Nebraska
More informationINTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE Q5B
INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE ICH HARMONISED TRIPARTITE GUIDELINE QUALITY OF BIOTECHNOLOGICAL PRODUCTS: ANALYSIS
More informationShop! VWRBiosciences,more than just a helping hand
section line 2 BioSciences section line 1 VWRBiosciences,more than just a helping hand Proteomics round-up What can we offer? In today s world of discovery, technology is critical to a better understanding
More informationAdvanced BioDesign Outlines Solutions. Antibody Overview. by Advanced BioDesign. Project Start. Immunogenicity. Selecting Your Antigen
Advanced BioDesign Outlines Solutions by Advanced BioDesign Antibody Overview Launching an immunisation programme is an important experimental step that needs care. With Advanced BioDesign, you may develop
More informationEuropean Medicines Agency
European Medicines Agency July 1996 CPMP/ICH/139/95 ICH Topic Q 5 B Quality of Biotechnological Products: Analysis of the Expression Construct in Cell Lines Used for Production of r-dna Derived Protein
More informationProteinScape. Innovation with Integrity. Proteomics Data Analysis & Management. Mass Spectrometry
ProteinScape Proteomics Data Analysis & Management Innovation with Integrity Mass Spectrometry ProteinScape a Virtual Environment for Successful Proteomics To overcome the growing complexity of proteomics
More informationKMS-Specialist & Customized Biosimilar Service
KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal
More informationThe Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring
The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,
More informationFACULTY OF MEDICAL SCIENCE
Doctor of Philosophy Program in Microbiology FACULTY OF MEDICAL SCIENCE Naresuan University 171 Doctor of Philosophy Program in Microbiology The time is critical now for graduate education and research
More informationIntroduction to mass spectrometry (MS) based proteomics and metabolomics
Introduction to mass spectrometry (MS) based proteomics and metabolomics Tianwei Yu Department of Biostatistics and Bioinformatics Rollins School of Public Health Emory University September 10, 2015 Background
More information"Small and large molecules bioproduction by mammalian and microbial fermentation"
"Small and large molecules bioproduction by mammalian and microbial fermentation" H.-P. Meyer Bio/PharMOS 2004-23-25 March 2004 Symposium B Business Diversification in Life Science "Small and large molecules
More informationAB SCIEX TOF/TOF 4800 PLUS SYSTEM. Cost effective flexibility for your core needs
AB SCIEX TOF/TOF 4800 PLUS SYSTEM Cost effective flexibility for your core needs AB SCIEX TOF/TOF 4800 PLUS SYSTEM It s just what you expect from the industry leader. The AB SCIEX 4800 Plus MALDI TOF/TOF
More informationFACULTY OF MEDICAL SCIENCE
Doctor of Philosophy in Biochemistry FACULTY OF MEDICAL SCIENCE Naresuan University 73 Doctor of Philosophy in Biochemistry The Biochemistry Department at Naresuan University is a leader in lower northern
More informationGlobal and Discovery Proteomics Lecture Agenda
Global and Discovery Proteomics Christine A. Jelinek, Ph.D. Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences Middle Atlantic Mass Spectrometry Laboratory Global
More informationPRODUCTION AND QUALITY CONTROL OF MEDICINAL PRODUCTS DERIVED BY RECOMBINANT DNA TECHNOLOGY
PRODUCTION AND QUALITY CONTROL OF MEDICINAL PRODUCTS DERIVED BY RECOMBINANT DNA TECHNOLOGY Guideline Title Production and Quality Control of Medicinal Products derived by recombinant DNA Technology Legislative
More informationMultiQuant Software 2.0 for Targeted Protein / Peptide Quantification
MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification Gold Standard for Quantitative Data Processing Because of the sensitivity, selectivity, speed and throughput at which MRM assays can
More informationProteinPilot Report for ProteinPilot Software
ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Pow erful mass spectrometers like
More informationPep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics
Pep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics Ilan Beer Haifa Research Lab Dec 10, 2002 Pep-Miner s Location in the Life Sciences World The post-genome era - the age of proteome
More informationExpression and Purification of Recombinant Protein in bacteria and Yeast. Presented By: Puspa pandey, Mohit sachdeva & Ming yu
Expression and Purification of Recombinant Protein in bacteria and Yeast Presented By: Puspa pandey, Mohit sachdeva & Ming yu DNA Vectors Molecular carriers which carry fragments of DNA into host cell.
More informationPlant Metabolomics. For BOT 6516
Plant Metabolomics For BOT 6516 Introduction Modern metabolomics began about ten years ago and yet many continue to question the relative performance of this area of technology in advancing plant biology.
More informationPharmacology skills for drug discovery. Why is pharmacology important?
skills for drug discovery Why is pharmacology important?, the science underlying the interaction between chemicals and living systems, emerged as a distinct discipline allied to medicine in the mid-19th
More informationThermo Scientific PepFinder Software A New Paradigm for Peptide Mapping
Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small
More informationCustom Antibodies & Recombinant Proteins
Custom Antibodies & Recombinant Proteins INTRODUCTION Custom services to meet your research and development requirements Improvements in health, medicine and diagnostics over the past century can be largely
More informationFor additional information on the program, see the current university catalog.
For information call: Tel: (818) 77-81 Fax: (818) 77-08 E-mail: chemistry.office@csun.edu Website: http://www.csun.edu/chemistry Or write: Department of Chemistry and Biochemistry California State University,
More informationHRMS in Clinical Research: from Targeted Quantification to Metabolomics
A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier
More informationIn-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates
In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics
More informationExciting Trends in Bioprocessing
Exciting Trends in Bioprocessing Alfred Doig and Susan Dana Jones, Ph.D. April 20, 2015 BioProcess Technology Consultants, Inc. 12 Gill Street, Suite 5450 Woburn, MA 01801 Exciting Trends in Bioprocessing
More informationBachelor of Science in Applied Bioengineering
Bachelor of Science in Applied Bioengineering Applied bioengineering employs scientific and engineering principles to process biological materials from biological agents for provision of goods and related
More informationBiophysical and biochemical mechanisms of the biological effects of mobile phone radiation
Biophysical and biochemical mechanisms of the biological effects of mobile phone radiation Dariusz Leszczynski Research Professor; STUK - Radiation and Nuclear Safety Authority Helsinki, Finland & Guangbiao
More informationHow To Understand How Gene Expression Is Regulated
What makes cells different from each other? How do cells respond to information from environment? Regulation of: - Transcription - prokaryotes - eukaryotes - mrna splicing - mrna localisation and translation
More informationTransfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.
Transfection Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide,
More information泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics
泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 2014 Training Course Wei-Hung Chang ( 張 瑋 宏 ) ABRC, Academia
More informationProviding Trusted and Innovative Solutions t o the Life Science Communities
Providing Trusted and Innovative Solutions t o the Life Science Communities Eurogentec Eurogentec is a leading supplier of trusted and innovative reagents, kits, specialty products and custom research/development
More informationLFB GROUP & SANOFI combine their bioproduction capabilities to provide integrated CMO services for biopharmaceuticals
LFB GROUP & SANOFI combine their bioproduction capabilities to provide integrated CMO services for biopharmaceuticals MAbLaunch TM is a joint bioproduction platform combining LFB Biomanufacturing (LFB
More informationM.Sc. in Nano Technology with specialisation in Nano Biotechnology
M.Sc. in Nano Technology with specialisation in Nano Biotechnology Nanotechnology is all about designing, fabricating and controlling materials, components and machinery with dimensions on the nanoscale,
More informationTutorial for Proteomics Data Submission. Katalin F. Medzihradszky Robert J. Chalkley UCSF
Tutorial for Proteomics Data Submission Katalin F. Medzihradszky Robert J. Chalkley UCSF Why Have Guidelines? Large-scale proteomics studies create huge amounts of data. It is impossible/impractical to
More information3rd Annual Modern Challenges in Therapeutic Protein Production Event The Penridge Suite, London, N11 1NL, UK : Thursday, 14 June 2012
3rd Annual Modern Challenges in Therapeutic Protein Production Event The Penridge Suite, London, N11 1NL, UK : Thursday, 14 June 2012 The purpose of this annual event is to look at the challenges facing
More informationAiping Lu. Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn
Aiping Lu Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Proteome and Proteomics PROTEin complement expressed by genome Marc Wilkins Electrophoresis. 1995. 16(7):1090-4. proteomics
More informationDMPK: Experimentation & Data
DMPK: Experimentation & Data Interpretation Mingshe Zhu, Mike S. Lee, Naidong Weng, and Mark Hayward Prerequisite: Entry-level scientists with hands on experience in LC/MS as well as advanced students
More informationCUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation
CUSTOM ANTIBODIES Highly competitive pricing without compromising quality. Rat monoclonal antibodies for the study of gene expression and proteomics in mice and in mouse models of human diseases available.
More informationMRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics
MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP and TripleTOF 5600 System Technology Targeted peptide quantification is a rapidly growing application
More informationAbsolute quantification of low abundance proteins by shotgun proteomics
Absolute quantification of low abundance proteins by shotgun proteomics Dr. Stefanie Wienkoop www.proteomefactory.com In cooperation with: Max-Planck-Institut für Molekulare Pflanzenphysiologie Stable
More informationIntroduction to Proteomics
Introduction to Proteomics Åsa Wheelock, Ph.D. Division of Respiratory Medicine & Karolinska Biomics Center asa.wheelock@ki.se In: Systems Biology and the Omics Cascade, Karolinska Institutet, June 9-13,
More informationMOLECULAR PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
MOLECULAR PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS R. M. Weinshilboum, M.D., Program Director L. Wang, M.D., Ph.D., Program Co-Director D. C. Mays, Ph.D., Associate Program Director Ph.D. Degree Course
More informationLFB GROUP & SANOFI combine their bioproduction capabilities to provide integrated CMO services for biopharmaceuticals
LFB GROUP & SANOFI combine their bioproduction capabilities to provide integrated CMO services for biopharmaceuticals MAbLaunch TM is a joint bioproduction platform combining LFB Biomanufacturing (LFB
More informationM110.726 The Nucleus M110.727 The Cytoskeleton M340.703 Cell Structure and Dynamics
of Biochemistry and Molecular Biology 1. Master the knowledge base of current biochemistry, molecular biology, and cellular physiology Describe current knowledge in metabolic transformations conducted
More informationINTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE Q6B. Current Step 4 version
INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE ICH HARMONISED TRIPARTITE GUIDELINE SPECIFICATIONS: TEST PROCEDURES AND ACCEPTANCE
More informationBIOTECHNOLOGY. Bouvé College of Health Sciences College of Science. Networked for Success. Networked for Industry. Networked for Life.
MASTER OF SCIENCE BIOTECHNOLOGY Networked for Success. Networked for Industry. Networked for Life. Bouvé College of Health Sciences College of Science Northeastern s Biotechnology Masters program synthesizes
More informationResearch-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE
Research-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE Jacob D. Jaffe Skyline Webinar July 2015 Proteomics and
More informationBIOENGINEERING. BACCALAUREATE PROGRAM This program leads to the degree, Bachelor of Science in Engineering with a major in Bioengineering
BIOENGINEERING BACCALAUREATE PROGRAM This program leads to the degree, Bachelor of Science in Engineering with a major in Bioengineering College of Engineering and Computing MIAMI UNIVERSITY 2014-2015
More informationName Class Date. Figure 13 1. 2. Which nucleotide in Figure 13 1 indicates the nucleic acid above is RNA? a. uracil c. cytosine b. guanine d.
13 Multiple Choice RNA and Protein Synthesis Chapter Test A Write the letter that best answers the question or completes the statement on the line provided. 1. Which of the following are found in both
More informationYour partner in immunology
Your partner in immunology Expertise Expertise Reactivity Reactivity Quality Quality Advice Advice Who are we? Specialist of antibody engineering Covalab is a French biotechnology company, specialised
More informationCURRICULUM VITAE. Yong-Cheol Park, Ph.D.
CURRICULUM VITAE Yong-Cheol Park, Ph.D. Postdoctoral Research Associate Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005-1892, U.S.A. Tel) 1-713-348-3304, Fax) 1-713-348-5154,
More informationTECHNOLOGIES, PRODUCTS & SERVICES for MOLECULAR DIAGNOSTICS, MDx ABA 298
DIAGNOSTICS BUSINESS ANALYSIS SERIES: TECHNOLOGIES, PRODUCTS & SERVICES for MOLECULAR DIAGNOSTICS, MDx ABA 298 By ADAMS BUSINESS ASSOCIATES MAY 2014. May 2014 ABA 298 1 Technologies, Products & Services
More informationIntroduction to Proteomics
Introduction to Proteomics Why Proteomics? Same Genome Different Proteome Black Swallowtail - larvae and butterfly Biological Complexity Yeast - a simple proteome 6,113 proteins = 344,855 tryptic peptides
More informationThe National Centre for Biomedical Engineering Science
The How this Integrated Technology Platform has impacted the development of R&D Clusters in Galway Jacinta Thornton, PhD Executive Manager, NCBES National Centre for Biomedical Engineering g Science Mission
More informationBio-Reagents Gene synthesis Peptide Synthesis Protein Expression Antibody Production. Life Science Products and Services
Bio-Reagents Gene synthesis Peptide Synthesis Protein Expression Antibody Production Life Science Products and Services Since 2002, Biomatik has provided worldwide researchers in life science discovery
More informationMolecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION
Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN
More informationusing ms based proteomics
quantification using ms based proteomics lennart martens Computational Omics and Systems Biology Group Department of Medical Protein Research, VIB Department of Biochemistry, Ghent University Ghent, Belgium
More informationChapter 3. Protein Structure and Function
Chapter 3 Protein Structure and Function Broad functional classes So Proteins have structure and function... Fine! -Why do we care to know more???? Understanding functional architechture gives us POWER
More informationTranscription and Translation of DNA
Transcription and Translation of DNA Genotype our genetic constitution ( makeup) is determined (controlled) by the sequence of bases in its genes Phenotype determined by the proteins synthesised when genes
More informationSession 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists
Program Overview Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Session 2. Principles of Mass Spectrometry Session 3. Mass spectrometry based proteomics
More informationHigh resolution mass spectrometry (HRMS*) in Graz
High resolution mass spectrometry (HRMS*) in Graz 10.2.2012 * Instruments with high resolution where exact mass measurements can be performed 1 HRMS in Graz s and Ionization Techniques 2 6 3 5 4 1 TU Graz
More informationBuilding innovative drug discovery alliances. Evotec Munich. Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs
Building innovative drug discovery alliances Evotec Munich Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs Evotec AG, Evotec Munich, June 2013 About Evotec Munich A leader
More informationIncreasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays
Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Scheduled MRM HR Workflow on the TripleTOF Systems Jenny Albanese, Christie Hunter AB SCIEX, USA Targeted quantitative
More informationMaster course KEMM03 Principles of Mass Spectrometric Protein Characterization. Exam
Exam Master course KEMM03 Principles of Mass Spectrometric Protein Characterization 2010-10-29 kl 08.15-13.00 Use a new paper for answering each question! Write your name on each paper! Aids: Mini calculator,
More informationICH Topic Q 6 B Specifications: Test Procedures and Acceptance Criteria for Biotechnological/Biological Products. Step 5
European Medicines Agency September 1999 CPMP/ICH/365/96 ICH Topic Q 6 B Specifications: Test Procedures and Acceptance Criteria for Biotechnological/Biological Products Step 5 NOTE FOR GUIDANCE ON SPECIFICATIONS:
More informationLa Protéomique : Etat de l art et perspectives
La Protéomique : Etat de l art et perspectives Odile Schiltz Institut de Pharmacologie et de Biologie Structurale CNRS, Université de Toulouse, Odile.Schiltz@ipbs.fr Protéomique et Spectrométrie de Masse
More informationBiochemistry Major Talk 2014-15. Welcome!!!!!!!!!!!!!!
Biochemistry Major Talk 2014-15 August 14, 2015 Department of Biochemistry The University of Hong Kong Welcome!!!!!!!!!!!!!! Introduction to Biochemistry A four-minute video: http://www.youtube.com/watch?v=tpbamzq_pue&l
More informationProtein Expression and Analysis. Vijay Yajnik, MD, PhD GI Unit MGH
Protein Expression and Analysis Vijay Yajnik, MD, PhD GI Unit MGH Identify your needs Antigen production Biochemical studies Cell Biology Protein interaction studies including proteomics Structural studies
More informationWhite Paper. Yeast Systems Biology - Concepts
White Paper Yeast Systems Biology - Concepts Stefan Hohmann, Jens Nielsen, Hiroaki Kitano (see for further contributers at end of text) Göteborg, Lyngby, Tokyo, February 2004 1 Executive Summary Systems
More informationMethods for Protein Analysis
Methods for Protein Analysis 1. Protein Separation Methods The following is a quick review of some common methods used for protein separation: SDS-PAGE (SDS-polyacrylamide gel electrophoresis) separates
More informationMolecular Biology And Biotechnology
in Molecular Biology And Biotechnology National Institute of Molecular Biology and Biotechnology College of Science University of the Philippines Diliman, Quezon City 1101 National Institute of Molecular
More informationMAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project
Mabsolus-2015-UK:Mise en page 1 03/07/15 14:13 Page1 Services provider Department of MABSolys from conception to validation MAB Solut is involved at each stage of your project Creation of antibodies Production
More informationProtein Expression. A Practical Approach J. HIGGIN S
Protein Expression A Practical Approach S. J. HIGGIN S B. D. HAMES List of contributors Abbreviations xv Xvi i 1. Protein expression in mammalian cell s Marlies Otter-Nilsson and Tommy Nilsso n 1. Introduction
More informationBiotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College
Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Primary Source for figures and content: Eastern Campus Tortora, G.J. Microbiology
More informationGenetics Lecture Notes 7.03 2005. Lectures 1 2
Genetics Lecture Notes 7.03 2005 Lectures 1 2 Lecture 1 We will begin this course with the question: What is a gene? This question will take us four lectures to answer because there are actually several
More informationClinical Research Infrastructure
Clinical Research Infrastructure Enhancing UK s Clinical Research Capabilities & Technologies At least 150m to establish /develop cutting-edge technological infrastructure, UK wide. to bring into practice
More informationBiotechpharma company profile
Biotechpharma company profile October 2013 1 History 2004 Biotechpharma UAB established as a proteomic research company in Vilnius, Lithuania 2005 Company became a member of UK s Northway group, investing
More informationBiological cell membranes
Unit 14: Cell biology. 14 2 Biological cell membranes The cell surface membrane surrounds the cell and acts as a barrier between the cell s contents and the environment. The cell membrane has multiple
More informationEnzyme Response Profiling: Integrating proteomics and genomics with xenobiotic metabolism and cytotoxicity
Enzyme Response Profiling: Integrating proteomics and genomics with xenobiotic metabolism and cytotoxicity Proteomics high throughput separation and characterization of expressed proteins in tissue and
More informationBiochemistry. Entrance Requirements. Requirements for Honours Programs. 148 Bishop s University 2015/2016
148 Bishop s University 2015/2016 Biochemistry The Biochemistry program at Bishop s is coordinated through an interdisciplinary committee of chemists, biochemists and biologists, providing students with
More informationQuantitative and Qualitative Systems Biotechnology: Analysis Needs and Synthesis Approaches
Quantitative and Qualitative Systems Biotechnology: Analysis Needs and Synthesis Approaches Vassily Hatzimanikatis Department of Chemical Engineering Northwestern University Current knowledge of biological
More informationCHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA
CHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA INTRODUCTION DNA : DNA is deoxyribose nucleic acid. It is made up of a base consisting of sugar, phosphate and one nitrogen base.the
More informationProSightPC 3.0 Quick Start Guide
ProSightPC 3.0 Quick Start Guide The Thermo ProSightPC 3.0 application is the only proteomics software suite that effectively supports high-mass-accuracy MS/MS experiments performed on LTQ FT and LTQ Orbitrap
More informationModule 3 Questions. 7. Chemotaxis is an example of signal transduction. Explain, with the use of diagrams.
Module 3 Questions Section 1. Essay and Short Answers. Use diagrams wherever possible 1. With the use of a diagram, provide an overview of the general regulation strategies available to a bacterial cell.
More informationNUVISAN Pharma Services
NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!
More informationAn Overview of Cells and Cell Research
An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism
More informationBiotechnology and Life Science Marketing Services Mailing List and Data Card Order Form
C H I Cambridge Healthtech Institute s Biotechnology and Life Science Marketing Services Mailing List and Data Card Order Form Over 800,000 names segmented by scientific interest Featuring U.S and International
More informationProteomics in Practice
Reiner Westermeier, Torn Naven Hans-Rudolf Höpker Proteomics in Practice A Guide to Successful Experimental Design 2008 Wiley-VCH Verlag- Weinheim 978-3-527-31941-1 Preface Foreword XI XIII Abbreviations,
More informationError Tolerant Searching of Uninterpreted MS/MS Data
Error Tolerant Searching of Uninterpreted MS/MS Data 1 In any search of a large LC-MS/MS dataset 2 There are always a number of spectra which get poor scores, or even no match at all. 3 Sometimes, this
More informationAccelerating drug development to FTIH: Potential of new expression technologies
Accelerating drug development to FTIH: Potential of new expression technologies Lekan Daramola Associate Director Biopharmaceutical Development, Cell Culture & Fermentation Sciences CMC Strategy Forum
More informationA Novel Bioconjugation Technology
A Novel Bioconjugation Technology for Assay Development and More! Presentation overview Who we are Solutions we provide for our customers Solulink s technology Linking system The Solulink advantage Applications
More informationFOR TEXTILES. o Kuchipudi-I] textiles. Outdoor Activity Based Courses. Camp
SEMESTER I Basic Sciences Courses o General Chemistry o Biology for Engineers o Fundamentals of Mathematics o Applied Physics-I for Life Science Behavioural Science o Understanding Self for Effectiveness
More informationMicroarray Technology
Microarrays And Functional Genomics CPSC265 Matt Hudson Microarray Technology Relatively young technology Usually used like a Northern blot can determine the amount of mrna for a particular gene Except
More information1 Mutation and Genetic Change
CHAPTER 14 1 Mutation and Genetic Change SECTION Genes in Action KEY IDEAS As you read this section, keep these questions in mind: What is the origin of genetic differences among organisms? What kinds
More information