Analytical Methods Approved for Compliance Monitoring under the Ground Water Rule
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1 Analytical Approved for Compliance Monitoring under the Ground Water Rule Analysis for the following contaminants shall be conducted in accordance with the methods in the following table or their equivalent as determined by. The methods and monitoring requirements for these contaminants are specified in 40 CFR and Additional methods are listed in Appendix A to Subpart C of Part 141. The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods information published in 40 CFR 141. If you find discrepancies, please notify The Safe Drinking Water Hotline ( ) so that can correct the table. Microbial Contaminants Escherichia coli The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10 C during transit. A standard sample volume of at least 100 ml must be collected for fecal indicator analysis regardless of the fecal indicator or analytical method used Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium) 9221 F for the Examination 9222 G for the Examination September R This method incorporates MI agar into Method 9222 as described for the analysis of Total Coliforms in the Total Coliform Rule [141.21(f)] EC-MUG (Method 9221 F) or NA-MUG (Method 9222 G) can be used for E. coli testing step as described in (f)(6)(i) or (iii) after use of 9221 B, 9221 D, 9222 B, or 9222 C EC-MUG (Method 9221 F) or NA-MUG (Method 9222 G) can be used for E. coli testing step as described in (f)(6)(i) or (iii) after use of 9221 B, 9221 D, 9222 B, or 9222 C for the Examination 9223 for the Examination 1998 Colilert, Colisure, and Colilert-18 media are approved for use Colilert, Colisure, and Colilert-18 media are approved for use. Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 1 of 10
2 Microbial Contaminants Escherichia coli The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10 C during transit. A standard sample volume of at least 100 ml must be collected for fecal indicator analysis regardless of the fecal indicator or analytical method used B-97 version of for Wastewater. Approval year by Committee is designated by Colilert, Colisure, and Colilert-18 media are approved for use. E*Colite Test Charm Sciences, Inc. Charm E*Colite Test, Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water January 9, 1998 Charm Sciences, Inc m-coliblue24 Test Hach Co. m-coliblue 24 Test, "Total Coliforms and E. coli Membrane Filtration Method with m-coliblue 24 Broth," Method No , Revision 2. August 17, 1999 Hach Company Enterococci The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10 C during transit. A standard sample volume of at least 100 ml must be collected for fecal indicator analysis regardless of the fecal indicator or analytical method used Enterococci in Water by Membrane Filtration Using membrane- Enterococcus Indoxyl-B-D-Glucoside Agar (mei) September R Method 1600 is an approved variation of Method 9230 C. The holding time and temperature for ground water samples are specified in the rule (see above note), rather than as specified in Section 8 of Method B for the Examination 9230 C for the Examination Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 2 of 10
3 Microbial Contaminants Enterococci The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10 C during transit. A standard sample volume of at least 100 ml must be collected for fecal indicator analysis regardless of the fecal indicator or analytical method used. Enterolert IDEXX Laboratories, Inc. Budnick, G.E., et.al, Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters, Applied and Environmental Microbiology, 62: IDEXX Laboratories, Inc. Coliphage The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10 C during transit. A standard sample volume of at least 100 ml must be collected for fecal indicator analysis regardless of the fecal indicator or analytical method used Male-specifice (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure April R Male-specifice (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure April R Free Chlorine If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, Free Chlorine Species (HOCl- and OCl-) by Test Strip, Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC for the Examination 1992 for the Examination 1995 for the Examination 1998 for the Examination 2005 Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 3 of 10
4 Free Chlorine If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, Free Chlorine Species (HOCl- and OCl-) by Test Strip, Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC version of for Wastewater. Approval year by Committee is designated by for the Examination 1992 for the Examination 1995 for the Examination 1998 for the Examination version of for Wastewater. Approval year by Committee is designated by for the Examination 1992 for the Examination 1995 for the Examination 1998 Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 4 of 10
5 Free Chlorine If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, Free Chlorine Species (HOCl- and OCl-) by Test Strip, Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC for the Examination version of for Wastewater. Approval year by Committee is designated by 4500-Cl H for the Examination Cl H for the Examination Cl H for the Examination Cl H for the Examination Cl H-00 version of for Wastewater. Approval year by Committee is designated by D ASTM Annual Book of ASTM s, Vol. International D ASTM Annual Book of ASTM s, Vol. International Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 5 of 10
6 Total Chlorine for the Examination 1992 for the Examination 1995 for the Examination 1998 for the Examination version of for Wastewater. Approval year by Committee is designated by 4500-Cl E for the Examination Cl E for the Examination Cl E for the Examination Cl E for the Examination Cl E-00 version of for Wastewater. Approval year by Committee is designated by Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 6 of 10
7 Total Chlorine for the Examination 1992 for the Examination 1995 for the Examination 1998 for the Examination version of for Wastewater. Approval year by Committee is designated by for the Examination 1992 for the Examination 1995 for the Examination 1998 for the Examination version of for Wastewater. Approval year by Committee is designated by Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 7 of 10
8 Total Chlorine 4500-Cl I for the Examination Cl I for the Examination Cl I for the Examination Cl I for the Examination Cl I-00 version of for Wastewater. Approval year by Committee is designated by D ASTM Annual Book of ASTM s, Vol. International D ASTM Annual Book of ASTM s, Vol. International Chlorine Dioxide 327 Rev 1.1 Determination of Chlorine Dioxide and Chlorite Ion in Drinking Water Using Lissamine Green B and Horseradish Peroxidase with Detection by Visible Spectrophotometry May R ClO2 C for the Examination 1992 Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 8 of 10
9 Chlorine Dioxide 4500-ClO2 C for the Examination ClO2 C for the Examination ClO2 C for the Examination ClO2 C-00 version of for Wastewater. Approval year by Committee is designated by 4500-ClO2 D for the Examination ClO2 D for the Examination ClO2 D for the Examination ClO2 E for the Examination ClO2 E for the Examination ClO2 E for the Examination ClO2 E for the Examination 2005 Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 9 of 10
10 Chlorine Dioxide 4500-ClO2 E-00 version of for Wastewater. Approval year by Committee is designated by Ozone 4500-O3 B for the Examination 4500-O3 B for the Examination 4500-O3 B for the Examination 4500-O3 B for the Examination 4500-O3 B-97 version of for Wastewater. Approval year by Committee is designated by Contact information for methods that are not available on the Internet are summarized in the report titled "Sources of Approved Analytical for National Drinking Water Regulations." Approved for the Ground Water Rule - National Primary Drinking Water Regulations Page 10 of 10
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