INTRODUCTION TO PLANT TISSUE CULTURE

Transcription

1 INTRODUCTION TO PLANT TISSUE CULTURE Preliminary Workshop Program Dr. Mike Kane, Gainesville, Florida July 12-15, 2010

2 INTRODUCTION TO PLANT TISSUE CULTURE A FOUR-DAY WORKSHOP July 12-15, 2010 Dr. Mike Kane Gainesville, FL WORKSHOP OVERVIEW The overall objective of the workshop is to provide instructional resources, conceptual background information and hands-on laboratory experiences to facilitate the incorporation of plant tissue culture (micropropagation) into classroom curricula in the most cost efficient manner. PARTICIPANTS Primary focus is on grade school science, vocational agriculture and college faculty. However, depending on availability of space, non-teachers wanting a hands-on experience will be allowed to register. INSTRUCTIONAL OBJECTIVES Upon completion participants will: Understand the principles and concepts of plant tissue culture, specifically micropropagation Be familiar with the laboratory and greenhouse procedures and equipment used to propagate plants using micropropagation Have instructional materials including PowerPoint lectures, laboratory exercises and other informational resources that can be used in the classroom Know where to obtain supplies to economically and successfully teach plant tissue culture in the classroom Receive specialized, hands-on training completing several reliable laboratory exercises Observe the commercial application of the technology by visiting a commercial plant tissue culture laboratory

3 PRELIMINARY PROGRAM Monday, July 12, :00-8:30 a.m. Workshop Registration (Lobby Fifield Hall) LECTURE SESSIONS (2316 Fifield Hall) 8:30-8:40 a.m. Welcome & Introductions 8:40 8:55 a.m. Opportunities in Environmental Horticulture 8:55 9:00 a.m. Workshop Goals & Objectives 9:00 9:30 am. Plant Tissue Culture: A Historical Perspective 9:30 10:00 a.m. Micropropagation Methods: Shoot Culture 10:00 10:20 a.m. Break refreshments provided 10:20 11:00 a.m. Micropropagation Methods: Shoot Culture (continued) 11:00 12:00 p.m. Plant Tissue Culture: Instructional Resources 12:00-1:00 p.m. Lunch provided LAB SESSIONS (Building 68, In Vitro Plant Culture Lab) 1:00 2:30 p.m. Laboratory Equipment and Sterile Technique 2:30 2:50 p.m. Break Refreshments provided 2:50 5:30 p.m. Starting Plant Tissue Cultures (Syngonium) Dinner (on your own) Tuesday, July 13, 2010 LECTURE SESSIONS (2316 Fifield Hall) 8:30 10:00 a.m. Micropropagation Methods: Shoot Organogenesis and Non-zygotic Embryogenesis 10:00 10:20 a.m. Break refreshments provided 10:20 12:00 pm. The Nuts & Bolts of Plant Tissue Culture Media 12:00 1:00 p.m. Lunch Provided LAB SESSIONS (Building 68, In Vitro Plant Culture Lab) 1:00 2:15 p.m. Shoot Organogenesis Parrot-feather 2:15-2:35 p.m. Break refreshments provided in Building 68) 2:35 3:30 p.m. In Vitro Flowering of Miniature Rose

4 3:30-5:00 p.m. How to Prepare Plant Tissue Culture Media 6:15 8:00 p.m. BBQ Dinner provided (Greenhouse Complex) Wednesday, July 14, 2010 LAB SESSIONS (Building 68, Plant in Vitro Culture Lab) 8:00 10:00 a.m. Micropropagation Using Floral Tissues (Cauliflower) 10:00 10:20 p.m. Break refreshments provided 10:20 12:00 p.m. Using Commercially Available Plant Tissue Culture Kits 12:00 1:00 p.m. Lunch provided 1:00 3:30 p.m. Orchid Seed Culture 3:30 3:45 p.m. Break refreshments provided 3:45 5:00 p.m. Synthetic Seed Technology Dinner (on your own) Thursday, July 15, 2010 COMMERCIAL MICROPROPAGATION LABORATORY TOUR 8:00 a.m. Depart from in front of Fifield Hall (van provided) 10:15 12:00 p.m. Commercial Tissue Culture Lab Tour, Apopka, Florida 12:00 1:30 p.m. Lunch (on your own) 1:30 2:30 p.m. Tour of UF Mid-Florida Research & Education Center (Tentative) 3:30 3:45 p.m. Break 3:45 6:00 p.m. Return to Gainesville, evaluation and adjourn 1 If it is more convenient, some participants may want to drive their own vehicles to Apopka and depart for home after the tour.

5 REGISTRATION INFORMATION Enrollment in this workshop is limited to the first 16 participants, and registrations will be accepted on a first-come, first-served basis (see registration form below). The $650 registration fee includes a CD-ROM containing Power Point lecture notes, images, videotapes and laboratory exercises. Refreshment breaks and lunch (3 days) will be provided for participants. Participants are responsible for making their own hotel reservations (see below). HOTEL ACCOMMODATIONS Numerous hotels are available in the Gainesville area to provide guest room accommodations during the workshop. Arrangements have been made for a reduce rate at the Homewood Suites by Hilton ( ) which is located within 2 miles of the at 3333 SW 42rd Street, Gainesville, FL (see map). The rate of $89/night (not including local sales and occupancy taxes) includes a hot continental breakfast and a welcome to the manager s reception light dinner (Monday Thursday 5:30-7:30PM). Reservations must be received June 25, 2010; thereafter reservations will be taken on a space available basis only. When making your reservation please refer to the Plant Tissue Culture Workshop to obtain the room rate. CONTACT Dr. Michael Kane, Professor P.O. Box Gainesville, FL Tel: ext. 205 Cell: Fax: micropro@ufl.edu

6 REGISTRATION FORM INTRODUCTION TO PLANT TISSUE CULTURE A Four-Day Workshop July 12 15, 2010 Name: Address: Phone: Please include a check (credit cards not accepted) in the amount of $650 payable to: Send registration form and payment to: Dr. Michael Kane P.O. Box Gainesville, FL IMPORTANT NOTE: Enrollment in this workshop will be limited to the first 16 paid registrants. Prior to submitting the registration payment, please contact Dr. Kane either by (micropro@ufl.edu) or telephone (office: ext. 205; cell: ) to verify that the workshop is not full. Registration fees are used to purchase workshop materials. Please note that registration fees are non-refundable after Friday, June 9, 2010.

7 UNIVERSITY OF FLORIDA AREA MAP Lectures will be held in 2316 Fifield Hall Laboratories will be held in Rm 111, Building 68 Lecture 2316 Fifield IFAS Research Drive Teaching Lab Bldg. 68 Homewood Suites