More automation and standardization for cell therapy research. Stem Cell Therapy Solutions

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1 More automation and standardization for cell therapy research. Stem Cell Therapy Solutions Blood Banking Capillary Electrophoresis Centrifugation Flow Cytometry Genomics Lab Automation Lab Tools Particle Characterization BR-14420A

2 Where it all begins. Beckman Coulter offers the researcher a wide range of stem cell therapy workflow solutions from sample preparation to gene expression. We collaborate with you to understand your specific laboratory research requirements and create flexible solutions that meet your evolving needs. We integrate a variety of specialized reagents, instrumentation and software to help you automate, standardize and simplify key stem cell therapy processes. By offering these synergistic workflow-driven solutions we help you sort, expand and profile the stem cells you are studying. You get better performance with accurate and efficient results throughout the cell therapy research continuum. Sample Preparation Cell Sorting Cell Expansion Phenotypic Profile Digital Imaging Intracellular Signaling Single Cell PCR Gene Expression

3 Sample Preparation The cellular therapy process starts by getting samples into the appropriate format for subsequent analysis. This phase is critical to the accuracy, precision and reproducibility of the final result. Beckman Coulter enables you to automate your process by offering a full range of flexible and scalable systems. The Biomek line of Laboratory Automation Workstations offers the researcher flexible platforms with varying footprints to fit the researcher s throughput needs. Our dedicated applications experts create automated solutions for a wide variety of commercially available kits. With Biomek software, it is easy to configure any application to your specific needs. Biomek NX P Laboratory Automation Workstation* Cell Sorting Cell sorting is essential to investigators seeking to isolate a subpopulation of cells for further study or analysis. Beckman Coulter s Cell Sorters have set the standard with both functionality and leading innovation. They are designed to provide researchers improved productivity with an array of biosafety features. The high-speed, configurable systems have a wide range of laser selections and optimized collection optics. Standard performance includes unmatched single cell sorting accuracy and electronics that support sort rates validated to 70,000 events per second. The innovation continues today with the MoFlo Cell Sorters, the only true beadless drop delay determination and monitoring system that avoids the use of foreign particles during instrument setup. MoFlo XDP Cell Sorter** MoFlo Astrios Cell Sorter**

4 Expanding the future of stem cell research. Cell Expansion In order for stem cells, or even fully differentiated cell populations to be truly beneficial or useful, either therapeutically, as drug screening tools, or for core research purposes, expansion of cells is a prerequisite. An appropriate culture environment is critical to stem cell expansion efforts. The Quantum Cell Expansion System is the latest in automated cell expansion technology for growing adherent and suspension cells. The Quantum is a functionally closed system. With its unique hollow fiber bioreactor and incubator system design, it achieves predictable harvest rates. The Touch Screen allows users to set up, load, modify or run protocols with very little training. Additionally, the Quantum can easily be used for scale-up cell manufacturing. Quantum Cell Expansion System The Quantum Cell Expansion System is the future of cell culture. The days of the T-flasks are over. It has everything that is required to advance the robust bioprocessing of cells for both research and the clinic. Our first results with this advanced technology are highly promising, and coupled to the outstanding support that we have received from Beckman Coulter, I have absolutely no hesitations in recommending this revolutionary approach to other cell culture labs. Professor Chris Mason, MBBS PhD FRCSI FRCS Regenerative Medicine Bioprocessing Unit Advanced Centre for Biochemical Engineering University College London High Content Data Analysis Kaluza flow cytometry analysis software is a robust platform that provides innovative, simple data analysis along with novel multiparametric visualization tools. Kaluza supports supercomputing hardware from NVIDIA to enable real-time analysis of multimillion event files. With its effortless workflow and revolutionary speed, Kaluza allows researchers to gain deeper insights into the biology of cells in a variety of applications such as stem cell differentiation. Kaluza Analysis Software

5 Phenotype and Function Flow cytometry allows investigators to observe the physical and functional characteristics of cells using multiparametric analysis acquisitions of thousands of cells per second. The Gallios Flow Cytometer has advanced optics and electronics design, enabling researchers to acquire data from cells stained with up to 10 colors with a high degree of sensitivity and resolution at high data rates. In addition, the innovative forward scatter design enables superior resolution of submicron particles. Its quick and easy setup improves lab workflow and productivity; while its remote diagnostics and servicing minimizes downtime. The Gallios provides a superior solution for multi-color cellular analysis of rare and dim-staining populations. Gallios Flow Cytometer Sizing and Counting For sizing and counting aggregates of stem cells, known as embryoid bodies, the Multisizer 4 COULTER COUNTER is the ideal choice. No other cell counting system available today can handle these extremely large aggregates, which can be even larger than 100 microns. This is due to the Multisizer 4 s dynamic range and advanced electrical signal processing technology. Multisizer 4 COULTER COUNTER I am pleased with the ease of use, efficiency, and accuracy of the Multisizer. Its interchangeable apertures make it possible to measure both embryoid bodies formed in culture systems and embryoid bodies populations after spheroid dissociation in one instrument, even though they significantly differ in size. Richard Carpenedo, PhD Department of Biomedical Engineering Georgia Institute of Technology

6 More ways to analyze from cell function, to protein and gene expression. Digital Imaging Laser Scanning Cytometry (LSC) combines the advantages of flow cytometry, images analysis and automated fluorescence microscopy resulting in a new technology with synergistically added strengths. It provides unique solutions in the continuum from single fluorescence molecules in solutions to cellular interactions in tissues. Cytomery-based software techniques support the unsurpassed specimen and application flexibility. icys Research Imaging Cytometer*** Cell Signaling By understanding cell signaling, diseases may be treated effectively, so it is important for researchers to study how cells perceive and respond to their microenvironment. Beckman Coulter offers a comprehensive set of antibodies for the detection of the phosphorylation state of signaling proteins in cells via flow cytometry, which enables the researcher to generate precise, specific and quantitative measurements of the signaling response in individual cellular subpopulations. The researcher gets quantitative, cell-type specific data that enables the interrogation of signaling pathways in ways previously unattainable by Western blotting. Cell Lab Signaling Cytometry

7 Single Cell PCR Genetic analysis of stem cell populations is the key to obtaining deeper insight to proliferation pathways, potency or cancerogeneity. As cell populations are known to show heterogenity it is essential to carry out research on the single cell level in order to detect differences in subpopulations with statistical significance. This also provides a perfect tool for quality control of stem cell expansion. To facilitate this research, the isolation of a single, viable cell is necessary. The MoFlo XDP and MoFlo Astrios Cell Sorters, which combine historically stable sorting fluidics with leading-edge electronic engineering and proven performance, are easily capable of sorting single cells on the AmpliGrid single cell PCR system. The AmpliGrid System allows for highly sensitive and reproducible single cell PCR or gene expression analysis in order to expand the information content of immunophenotyping and imaging technologies. AmpliGrid System for single cell PCR and gene expression analysis Gene Expression The ability to capture a picture of cellular function is possible by looking at the expression profile of multiple genes at once. The profiles can distinguish between cells that are actively dividing or show how cells react to a particular treatment. The GeXP Genetic Analysis System offers researchers a quantitative high-throughput, multiplexed gene expression profiling solution, utilizing the XP-PCR technology. It analyzes up to 30 genes in a single well reaction with unrivaled sensitivity. The GeXP quantitatively detects small biologically relevant changes in gene expression, as low as 0.5 fold, with high accuracy. The platform scalability enables more target genes in each reaction and samples per run than RT-PCR, eliminating bottlenecks associated with your pluripotency or differentiation gene expression studies. GeXP* Genetic Analysis System

8 Customer Support We add more to your purchase than just instrumentation. You get software, consumables and excellent customer support. The finest quality customer assistance and expert technical service is just a phone call away. The implemented quality management systems are compliant with the requirements of ISO 9001 and/or ISO So you can be sure we adhere to the highest standards in the industry. AmpliGrid, icys, Gallios, Kaluza, MoFlo Astrios, and MoFlo XDP are for Research Use Only. Not for use in diagnostic procedures. * Biomek systems and GeXP are for Laboratory Use Only. Not for use in diagnostic procedures. Quantum is pending submission and clearance by regulatory agencies in Canada, France, Germany, Japan and Sweden. Quantum is a trademark or registered trademark of CaridianBCT, Inc. in the United States and in several other jurisdictions. ** MoFlo XDP and MoFlo Astrios Cell Sorters are class 1 lasers. *** icys is a registered trademark of the CompuCyte Corporation. NVIDIA is a registered trademark of NVIDIA Corporation. Beckman Coulter, the stylized logo, AmpliGrid, Biomek, MoFlo and COULTER are registered trademarks of Beckman Coulter, Inc. Astrios, Gallios, Kaluza and Multisizer are trademarks of Beckman Coulter, Inc. For Beckman Coulter s worldwide office locations and phone numbers, please visit Contact Us at B DG-5K 2011 Beckman Coulter, Inc. PRINTED IN U.S.A.