Laboratory Research Conduct & Safety: Biohazards and Biosafety. Environmental Health & Safety
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1 Laboratory Research Conduct & Safety: Biohazards and Biosafety
2 Guidelines & Standards Human Pathogens Public Health Agency of Canada Canadian Food Inspection Agency Animal Pathogens
3
4 Definition: Biohazards
5 Definition: WHMIS* CLASS AND SYMBOL CHARACTERISTICS PRECAUTIONS Class D Poisonous and Infectious Material Division 3: Biohazardous Infectious Materials Microbiological agents (e.g., bacteria, viruses, fungi and their toxins) that may cause illness or death Wear the recommended protective equipment and clothing Work with these materials in designated areas Disinfect area after handling Wash hands after handling *Workplace Hazardous Materials Information System
6 Definition: Biohazards Parasites Fungi Viruses Bacteria Rickettsiae Microbial toxins e.g., Anthrax edema factor, Botulinum toxin, Pertussis toxin Tetrotodotoxin
7 Definition: Biohazards Any material that could contain biohazardous material, for example: Human tissues, blood, body fluids Animal tissues, carcasses Cell lines Cultures from soil samples Waste water
8 Routes of Disease Transmission Direct contact (splash) Inoculation Ingestion Indirect contact Inhalation
9 Safe Work Practices Understand hazards Restrict lab access Avoid clutter Use personal protective equipment Lab coat Gloves Eye protection
10 McGill PPE Policy
11 Safe Work Practices (cont d) No shorts, sandals, etc. Observe Universal Precautions No food, drinks, etc.
12 Safe Work Practices (cont d) Work carefully, deliberately No mouth pipetting Use needles, syringes only if necessary Decontaminate work surfaces, hands Report accidents Cuts, needlesticks, scrapes Contamination of broken skin Splash of infectious material Incidents and near accidents
13 Definition: Risk Group Categorization of relative hazards of infective organisms (4 levels) Risk classification: Pathogenicity Infectious dose Mode of transmission Host range Effective prevention (e.g vaccine) Effective treatment (e.g., antibiotic, antiviral PEP)
14 Definition: Containment Level Minimum requirements for safe handling (4 levels) Operational practices Engineering, technical, physical: Location & access Surface finishes & casework HVAC Containment perimeter (windows, autoclave location, etc.) Services (water, drains, gas, electricity, equipment)
15 Risk Group 1 Low individual & community risk Unlikely to infect healthy humans/animals Containment Level 1 minimum Standard molecular biology organisms, e.g. E. coli K12, Lactobacillus spp., Bacillus subtilis, Micrococcus spp., Aerococcus spp.
16 Containment Level 1 No special design features BSC not required, may be used for sterility Work may be done on open benchtop Good microbiological technique
17 Risk Group 2 Moderate individual, low community risk Prevention or treatment available Spread unlikely Containment Level 2 minimum Salmonella typhimurium, Creutzfeldt-Jacob agent, Leishmania spp., Toxoplasma spp., Ascaris spp., human blood, eukaryotic cell cultures, enteropathogenic E. coli
18 Containment Level 2 Additional physical requirements, e.g. Limited access, signage, lockable doors Resistant, non-absorptive surfaces (for disinfection) Containment of aerosols: BSC Centrifuges with sealed rotors or safety cups Minimize environmental contamination: Handwashing sinks Decontamination facilities (autoclaves)
19 Risk Group 3 High individual, low community risk May cause serious disease Prevention and/or treatment available Unlikely to spread by casual contact Containment Level 3 minimum Lassa fever virus, hantavirus, Bacillus anthracis, Yersinia pestis, Histoplasma capsulatum, SARS, cultures of Hepatitis B or HIV
20 Risk Group 4 High individual, high community risk Readily transmitted (aerosol) Low infectious dose Very serious disease No treatment or preventive vaccine Containment Level 4 Marburg virus, Ebola virus, Herpesvirus simiae, Crimean-Congo hemorrhagic fever
21 Containment Levels 3 and 4 Level 3 Respiratory protection HEPA filtration of lab exhaust Strictly controlled access Level 4 Isolated facility with sealed perimeter Positive pressure suits or Class 3 BSC Effluent sterilization system
22
23 Public Health Agency of Canada (PHAC) MSDS for Infectious Substances Recommended Precautions Containment Protective clothing Other precautions (e.g. animal biosafety level) Handling Info Spills Disposal Storage Miscellaneous Preparation date Preparer s name
24 Biohazards Policy* Prior to beginning work with biohazardous material, responsible users must complete and submit an Application to Use Biohazardous Materials to Environmental Health & Safety for review and approval *University Laboratory Safety Committee September 24, 2007
25 Biohazards Applications Completed by PI/lab supervisor prior to starting project Required for research, testing, diagnostic activities using biohazards Required for all activities, including Level 1 Submit to EHS for approval (~1 week)
26 Thesis Preparation & Submission Guidelines* If the research for the thesis involved... microorganisms, living cells, other biohazards... the appropriate compliance certificates must be included as an appendix to the thesis *Graduate and Postdoctoral Studies:
27 Lab Safety Training Courses WHMIS Training for Lab Personnel Principles of Laboratory Radiation Safety Introduction to Biosafety Safe Use of Biological Safety Cabinets Hazardous Waste Management & Disposal Training for Lab Personnel
28 Contact information: Telephone: Fax: Website: Address: 3610 McTavish, 4 th floor
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