Curriculum Vitae. Huang, Chien-Jui. Post Doctoral Fellow, Department of Plant Pathology and Microbiology, National Taiwan University

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1 Curriculum Vitae Huang, Chien-Jui Post Doctoral Fellow, Department of Plant Pathology and Microbiology, Address: Room 402, New Plant Pathology Building, No.1, Sec. 4, Roosevelt Rd., Da-an District, Taipei, Taiwan, Tel: Date of Birth: June 23, 1978 Education Degree and Major Period Institute Ph.D., Plant Pathology and Microbiology Bs., Plant Pathology Experience Postition Period Institute Post Doctoral Fellow 2006-now Graduate research assistant Teaching assistant of Plant 2000 Pathology Teaching assistant of Biotechnology core techniques (4 times)

2 Research interests Post Doctoral Research: 1) Identification of elicitors produced by Bacillus cereus C1L to induce systemic resistance in plants. 2) Rhizobacteria-induced systemic resistance in plants against necrotrophic plant pathogen. 3) Development of practical methods and formulation for a biocontrol strain B. cereus C1L. 4) Application of bacterial chitinases on plant disease control. Doctoral Research: Chitinase system of antagonistic Bacillus cereus 28-9, including gene cloning, protein expression and biochemical characterization of two chitinases from B. cereus Honors 2010 IBC s TOP 100 Scientists 2010 Marquis who s Who in the World now Post Doctoral Fellowship from TOP 100 project of Ministry of Education 2003 A grant from Ministry of Education to support of Ph.D. student to join international congress 2002 Matsumoto Wei Scholarship Fellowship Taiwan Phytopathological Society Invited speak in international congress Bacillus cereus induces systemic resistance against Botrytis elliptica in lily. 90th annual meeting of Chinese Association of Agricultural Science Society. Beijing, China Chitinase system and its involvement in the antifungal activity of Bacillus cereus th International Congress on Molecular Plant-Microbe Interactions. St.-Petersburg, Russia.

3 Publications A. Papers 1. Huang, C. J., Yang, K. H., Liu, Y. H., and Chen, C. Y Efficacy of probenazole in inducing systemic resistance of maize against southern corn leaf blight. Pest Manag. Sci. In revision. (SCI, impact factor = 2.190, ranking 10.08% in Entomology) 2. Huang, C. J., Yang, K. H., Liu, Y. H., Lin, Y. J., and Chen, C. Y Suppression of southern corn leaf blight by a plant growth promoting rhizobacterium Bacillus cereus C1L. Ann. Appl. Biol. 157: (SCI, impact factor (2009) = 1.734, ranking 9.09% in Agriculture, Multidisciplinary) 3. Liu, Y. H., Huang, C. J., and Chen, C. Y Identification and transcriptional analysis of genes involved in Bacillus cereus-induced systemic resistance in Lilium. Biol. Plant. In press. Contributed equally to the first author. (SCI, impact factor (2009) = 1.656, ranking 34.88% in Plant Sciences) 4. Huang, C. J., Guo, S. H., Chung, S. C., Lin, Y. J., and Chen, C. Y Analysis of the involvement of chitin-binding domain of ChiCW in antifungal activity, and engineering a novel chimeric chitinase with high enzyme and antifungal activities. J. Microbiol. Biotechnol. 19: (SCI, impact factor (2007) = 2.062, ranking 47.83% in Biotechnology and Applied Microbiology) 5. Liu, Y. H., Huang, C. J. and Chen, C. Y Evidence of induced systemic resistance against Botrytis elliptica in lily. Phytopathology 98: (SCI, impact factor (2009) = 2.223, ranking 25.58% in Plant Sciences) 6. Huang, C. J. and Chen, C. Y Synergistic interactions between chitinase ChiCW and fungicides against plant fungal pathogens. J Microbiol Biotechnol. 18: (SCI, impact factor (2007) = 2.062, ranking 47.83% in Biotechnology and Applied Microbiology) 7. Liu, Y. H., Huang, C. J., Yang, K. H., and Chen, C. Y Effect of hydrogen peroxide on infection of lily by Botrytis elliptica. Plant Pathol. Bull. 17: Liu, Y. H., Huang, C. J. and Chen, C. Y Bacillus cereus induces systemic resistance against Botrytis elliptica in lily. Chin. Agri. Sci. Bull. 23: Huang, C. J. and Chen, C. Y Functions of the C-terminal region of chitinase ChiCW from Bacillus cereus 28-9 in substrate-binding and hydrolysis of chitin. J Microbiol Biotechnol. 16: (SCI, impact factor (2007) = 2.062, ranking 47.83% in Biotechnology and Applied Microbiology) 10. Huang, C. J. and Chen, C. Y High-level expression and characterization of two chitinases, ChiCH and ChiCW, of Bacillus cereus 28-9 in Escherichia coli. Biochem.

4 Biophys. Res. Commu. 327:8-17. (SCI, impact factor (2009) = 2.548, ranking 51.59% in Biochemistry and Molecular Biology) 11. Huang, C. J., Wang, T. K., Chung, S. C., and Chen, C. Y Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus J. Biochem. Mol. Biol. 38: (SCI, impact factor (2009) = 2.021, ranking 66.43% in Biochemistry and Molecular Biology) 12. Huang, C. J. and Chen, C. Y Gene cloning and biochemical characterization of chitinase CH from Bacillus cereus Ann. Microbiol. 54: (SCI, impact factor (2009) = 0.358, ranking 93.33% in Biotechnology and Applied Microbiology) 13. Chen, C. T., Huang, C. J., Wang, Y. H., and Chen, C. Y Two-step purification of Bacillus circulans chitinase A1 expressed in Escherichia coli periplasm. Protein Expr. Purif. 37: (SCI, impact factor (2009) = 1.563, ranking 62.00% in Biotechnology and Applied Microbiology) 14. Chen, C. Y., Wang, Y. H., and Huang, C. J Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chia gene. Can. J. Microbiol. 50: (SCI, impact factor = 1.262, ranking 68.67% in Biotechnology and Applied Microbiology) B. Books, Chapters, or Dissertation 1. Liu, Y. H., Huang, C. J., Lin, Y. J., and Chen, C. Y Activation of plant immunity for control of plant diseases. Proceedings of symposium on plant disease management in the era of energy-saving and carbon reduction. pp Taiwan Agricultural Research Institute and Taiwan Phytopathological Society. 2. Huang, C. J Chitinase system of antagonistic Bacillus cereus Ph.D. dissertation.. 3. Huang, C. J., Wang, T. K., Chung, S. C., and Chen, C. Y Chitinase system and its involvement in the antifungal activity of Bacillus cereus Biology of Plant- Microbe Interactions. pp IS-MPMI. C. Abstracts of Symposia 1. Huang, C. J., Yang, H. P., Chang, H. D., Tsai, C. F., and Chen, C. Y Plant growth promotion and disease suppression by a biocontrol strain of Bacillus cereus. The Canadian Phytopathological Society Annual Meeting.

5 2. Huang, C. J., Yang, K. H., Liu, Y. H., and Chen, C. Y Efficacy of probenazole in inducing systemic resistance of maize against southern corn leaf blight. Plant Pathol. Bull. (Abstr.) 3. Huang, C. J., Liu, Y. H., and Chen, C. Y Application of Bacillus cereus C1L to reduce the use of fungicide in plant disease control. Plant Pathol. Bull. (Abstr.) 4. Liu, Y. H., Huang, C. J., Lin, Y. J., and Chen, C. Y Activation of plant immunity for control of plant diseases. Symposium on plant disease management in the era of energy-saving and carbon reduction. Taichung, Taiwan, ROC. 5. Liu, Y. H., Huang, C. J., and Chen, C. Y Identification of genes involved in Botrytis cereus-induced systemic resistance in Lilium formosanum and the transcriptional analysis of gene expression. International Conference on Biotic Plant Interactions. Brisbane, Australia. 6. Lin, Y. J., Huang, C. J., Liu, Y. H., and Chen, C. Y Colonization of Bacillus cereus strain C1L on the roots of Lilium formosanum. Plant Pathol. Bull. (Abstr.) 7. Liu, Y. H., Huang, C. J. and Chen, C. Y Bacillus cereus induces systemic resistance against Botrytis elliptica in lily. 90th annual meeting of Chinese Association of Agricultural Science Society. Beijing, China. (Invited speaker) 8. Huang, R.Y., Huang, C. J., and Chen, C. Y Transposon mutagenesis in antagonistic bacterium Bacillus cereus C1L and the cloning of genes related to antifungal activity. 12th Symposium on Bacteria. Hsinchu. (Abstr.) 9. Liu, Y. H., Huang, C. J., and Chen, C. Y Evidence of Bacillus cereus induces systemic resistance against Botrytis elliptica in lily. XIII International Congress on Molecular Plant-Microbe Interactions. Sorrento, Italy. (Abstr.) 10. Huang, C. J. and Chen, C. Y A chitinase-overproducing strain of E. coli constructed for characterization of ChiCW from Bacillus cereus th International Microbial Genomics Conference. Lake Arrowhead, California. (Abstr.) 11. Huang, C. J., Wang, T. K., Chung, S. C., and Chen, C. Y Chitinase system and its involvement in the antifungal activity of Bacillus cereus th International Congress on Molecular Plant-Microbe Interactions. St.-Petersburg, Russia. (Invited speaker) 12. Huang, C. J. and Chen, C. Y Characterization of chitinases produced by an antagonistic bacterium, Bacillus cereus Plant Pathol. Bull. 10:214. (Abstr.)

6 D. Others :28-33

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