Screening of mycotoxins in food by triple quadrupole and QTRAP LC-MS/MS systems. Dr Stephen Lock

Transcription

1 Screening of mycotoxins in food by triple quadrupole and QTRAP LC-MS/MS systems Dr Stephen Lock

2 Structures of Analytes Mycotoxins C H 3 H O H O H OH OH O H O CH 3 O HO H HO CH 2 CH 3 OH H Zearalenon O DON Trichothecenes NIV, DON, FUS-X, 3AcDON, DAS, HT2, T2, Roridin A Zearalenone derivatives ZON Aflatoxins Aflatoxin B1, B2, G1, G2, M1, M2 Aflatoxin B1/M1 O O O OH O Ochratoxins C H 2 C H N H O CH 3 H Ochratoxin A Ochratoxin A Cl AB SCIEX

3 Regulations and needs of methods Regulations Any method needs to comply with regulations and these change with geography but are typically close to those observed in the EU (i.e. EC 1881/26 and the amendments EC 1126/27). Methods Need to combine aspects of recent EU reference methods (EN 1585:21 and EN 15851:21) into a single method to reduce analysis time and meets the performance of both methods Need to be applicable to different matrices e.g. baby food, grains and fruit and vegetables AB SCIEX

4 Sample Preparation Homogenized sample (2 g) was mixed with acetonitrile/water (8 ml, 8/2) and roller mixed for 2 minutes. The sample was centrifuged for 1 minutes at 35 rpm and filtered using a Phenomenex PHENEX filter (15 mm RC Membrane.45 µm). The filtrate was then diluted 1:4 with water containing 5 mm ammonium acetate prior to injection AB SCIEX

5 AB SCIEX QTRAP 55 System Fast scan speeds Fast pos / neg switching Robust and Rugged Turbo V Source High sensitivity AB SCIEX

6 Scheduled MRM Algorithm Time, min XICof +MRM(15pairs): 167.2/85.1amuExpectedRT:.6ID: Cyromazine1fromSample2(Std1)of Datareal samplesmrm_fast.wif. Max. 3.6cps. Target analytes are only detected around expected retention time Detection windows are automatically scheduled and adjusted Optimizes dwell times for each analyte and cycle time Allows detecting many more MRM transitions and use UHPLC AB SCIEX

7 Comparison of Scheduled MRM vs. MRM min Time, min Time, Intensity, cps Intensity, cps min Time, min Time, FUS X in negative polarity (left) and AFG1 in positive polarity (right) 1.2e4 1.e Scheduled MRM data points S/N = data points S/N = e4.. -MRM(12 pairs): Exp 1, / Max cps. XICof +MRM(24 pairs): Exp 2, 329./ Max. 1.3 cps. XICof MRM data points S/N = e4 6.e4 4.e4 9 data points S/N = e AB SCIEX

8 MRM conditions used Mycotoxin RT (min) Polarity Ion MRM (quantifier) MRM (qualifier) 15-Acetyldeoxynivalenol (15-AcDON) 3.7 positive [M+H] + 339/ /137 3-Acetyldeoxynivalenol (3-AcDON) 3.7 negative [M+CH3COO] - 397/37 397/59 negative [M-H] - 337/37 Aflatoxin B1 (AFB1) 4.2 positive [M+H] + 313/ /128 Aflatoxin B2 (AFB2) 4.1 positive [M+H] + 315/ /259 Aflatoxin G1 (AFG1) 4 positive [M+H] + 329/ /2 Aflatoxin G2 (AFG2) 3.9 positive [M+H] + 331/ /245 Deoxynivalenol (DON) 3 negative [M+CH 3 COO] - 355/ /59 Diacetoxyscirpenol (DAS) 4.2 positive [M+H] + 384/37 384/247 Fumonisin B1 (FB1) 4.8 positive [M+H] + 722/ /352 Fumonisin B2 (FB2) 5.1 positive [M+H] + 76/336 76/318 Fusarenon X (FUS X) 3.3 negative [M+CH 3 COO] - 413/ /59 HT-2 toxin 4.6 positive [M+NH 4 ] + 442/ /15 Monoacetoxyscirpenol (MAS) 3.9 positive [M+H] + 342/ /37 Nivalenol (NIV) 2.5 negative [M+CH 3 COO] - 371/ /59 Ochratoxin A (OTA) 5 positive [M+H] + 44/239 44/12 T-2 toxin 4.9 positive [M+NH 4 ] + 484/ /185 Zearalenon (ZON) 5.1 negative [M-H] - 317/ / AB SCIEX

9 HPLC Conditions Column was a Phenomenex Kinetex 2.6 μm XB-C18 (5x2.1 mm) Column temperature of 4 C Gradient of water to methanol with both phases containing 5 mm ammonium acetate and.5% acetic acid Time (min) Flow (μl/min) A (%) B (%) An injection volume of 3 μl AB SCIEX

10 535 AB SCIEX QTRAP 55 System me, mi n Ti me, mi n Ti Polarity Switching with the Scheduled MRM Algorithm eq Electronics DON 2.95 Negative polarity NIV 3-AcDON NIV FUS X XICof +MRM(24pairs): Exp2, 313. /285.2amuExpectedRT: 4.2ID: AFB11fromSample24(1in2) of a.max. 2.1cps. AFB Positive polarity e4 e4 e4 e4. AFG1 15-AcDON AFB2 MAS AFG2 DAS OTA HT-2 T-2 FB1 FB2 Mycotoxin detection in a single injection with polarity switching AB SCIEX

11 Calibration line for AFB1 Triplicate injections of AFB1 at.4 ng/ml with %CV of 4.2 (top) and calibration line for ZON.2 to 4 ng/ml with r =.9969 (bottom) Calibration for AFB1 1: y = x (r =.9969) (weighting: 1 / x) 1.e7 9.e6 8.e6 7.e6 6.e6 5.e6 4.e6 3.e6 2.e6 1.e6.e Concentration AB SCIEX

12 Calibration line for ZON Triplicate injections of ZON at.4 ng/ml with %CV of 9.2 (top) and calibration line.2 to 4 ng/ml with r =.9998 (bottom) Calibration for ZON 1: y = x (r =.9998) (weighting: 1 / x) e4.e Concentration AB SCIEX

13 1 in 1 left and 1 in 1 dilution cereal samples XIC of -MRM (12 pairs): Exp 1... Max cps. XIC of -MRM (12 pairs): Exp 1,... Max cps. XIC of -MRM (12 pairs): Exp 1,... Max cps Intensity, cps ZON - Baby cereal spiked at.4 ng/ml Intensity, cps ZON - Baby cereal blank Intensity, cps ZON - Baby cereal spiked at.4 ng/ml S/N = Time, min XIC of +MRM (24 pairs): Exp 2,... Max. 2.8e4 cps Time, min XIC of +MRM (24 pairs): Exp 2,... Max cps Time, min XIC of +MRM (24 pairs): Exp... Max cps e4 2.5e4 2.5e4 Intensity, cps 1.5e4 AFB1 - Baby cereal spiked at.4 ng/ml Intensity, cps 1.5e4 AFB1 - Baby cereal blank Intensity, cps 1.5e4 AFB1 - Baby cereal spiked at.4 ng/ml S/N = Time, min Time, min Time, min AB SCIEX

14 The next step in improving the detection limits for mycotoxins

15 AB SCIEX QTRAP 65 System Our most sensitive triple quadrupole with up to 1x increase in sensitivity and 2x increase in detector dynamic range all with no compromise in mass range combined with QTRAP technology AB SCIEX

16 AB SCIEX QTRAP 65 System Multi-component IonDrive Technology 1. IonDrive Turbo V ion source with increased heater diameter for improved ionization 2. Simulated gas dynamics of the curtain gas interface, acts as a better barrier against neutrals and micro droplets for increased robustness 3. IonDrive QJet ion guide with dual stage design to better capture ions from a larger orifice and better transmission into Q 4. IonDrive HED detector with an increased count rate of over 1 8 cps AB SCIEX

17 AB SCIEX QTRAP 65 System MRM Sensitivity Gains Verapamil AB Sciex QTRAP 65 system 6 x signal gain >4 x S/N gain vs. QTRAP 55 system QTRAP 55 system AB SCIEX

18 AB SCIEX QTRAP 65 System min Time, Aldosterone in Serum Triple Quad 65 system pg/ml Aldosterone 34 S/N = x Triple Quad 55 system 1 pg/ml Aldosterone S/N = AB SCIEX

19 535 Different mycotoxin response factors me, mi n Ti me, mi n Ti DON 2.95 Negative polarity NIV 3-AcDON NIV FUS X XICof +MRM(24pairs): Exp2, 313. /285.2amuExpectedRT: 4.2ID: AFB11fromSample24(1in2) of a.max. 2.1cps. AFB Positive polarity e4 e4 e4 e4. AFG1 15-AcDON AFB2 MAS AFG2 DAS OTA HT-2 T-2 FB1 FB AB SCIEX

20 AB SCIEX QTRAP 65 System e6. pg/ml Concentration, High Sensitivity and Improved Detector Provides up to 6 Orders of Linear Dynamic Range 1 μg/ml 1.5e8 1.4e8 1.3e8 1.2e8 1.1e8 1.e8 6 orders LDR! 9.e7 8.e7 7.e7 6.e7 Calibration for Aldosterone 1: y = e-4 x e-4 (r =.99971) (weighting: 1 / x) pg/ml 5.e7 4.e7 3.e pg/ml.15 2.e e7. 1 pg/ml Concentration Ratio AB SCIEX

21 Taking advantage of microflow LC Eksigent ekspert microlc 2 UHPLC system hybrid electrodes for microflow LC 25 µm ID 5 µm ID Pesticide Residue Analysis Component Name Retention Time (mins) Signal / Noise micro LC Signal / Noise Normal LC QuEChERS extraction 1 x dilution of extracts.5 x 5 mm Halo C18 4 µl/min using the gradient 2 μl injection Scheduled MRM Monocrotophos Tricyclazole Simetryn Monolinuron Isoproturon Terbutryn Flutolanil Fenoxycarb Pyridaben AB SCIEX

22 Robustness data from pesticides in chilli extracts e4 6.e4 4.e min Time, Calibration for Flutolanil 1: y = x (r =.99936) (weighting: None) 1.8e7 1.6e7 1.4e7 1.2e7 1.e e e e e6 2.e e Concentration Example of a calibration line for one of the pesticides, Flutolanil, from.2 1ppb.The fit used was Linear with 1/x and the r value obtained was greater than.99. This was typical for the pesticides used. Excellent robustness: peak area reproducibility for 3 selected pesticides (15 injections) Peak Area (cps).e Injection Number AB SCIEX 1.6E+5 1.4E+5 1.2E+5 1.E+5 8.E+4 6.E+4 4.E+4 2.E+4

23 Combination of Micro LC & AB SCIEX QTRAP 65 System More sensitivity in combination with extended Linear Dynamic Range means... More room for diluting the matrix effect away Less injections to cover analytes with very different sensitivities or MRL in multi methods Less solvent consumption => Savings in time and money AB SCIEX

24 You can now choose you LCMS system to match the degree of sample preparation AB SCIEX Triple Quad 45 AB SCIEX QTRAP 45 AB SCIEX Triple Quad 55 AB SCIEX QTRAP 55 AB SCIEX Triple Quad 65 AB SCIEX QTRAP AB SCIEX

25 Thank you for listening! AB SCIEX

26 Questions and Answers For Research Use Only. Not for use in Diagnostics Procedures. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX TM is being used under license AB SCIEX AB SCIEX