Dengue and Chikungunya virus van Duijl-Richter, Mareike

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1 Dengue and Chikungunya virus van Duijl-Richter, Mareike IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2016 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): van Duijl-Richter, M. (2016). Dengue and Chikungunya virus: Cell entry mechanisms and the impact of antibodies on infectivity [Groningen]: University of Groningen Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date:

2 Dengue and Chikungunya Virus Cell Entry Mechanisms and the Impact of Antibodies on Infectivity Mareike van Duijl-Richter

3 The research described in this thesis was primarily performed at the Department of Medical Microbiology of the University Medical Center Groningen (UMCG) within the Groningen University Institute for Drug Exploration (GUIDE). This work was financially supported by GUIDE and the Jan Kornelis de Kock foundation (Groningen). The printing of this thesis was financially supported by the University of Groningen and the Graduate School of Medical Sciences. Copyright 2015 Mareike van Duijl All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without written permission of the author and, when appropriate, the publisher holding the copyrights of the published articles. ISBN (hardcopy) (digital) Cover idea and design Mareike van Duijl Printed by NetzoDruk Groningen B.V., The Netherlands

4 Dengue and Chikungunya Virus Cell Entry Mechanisms and the Impact of Antibodies on Infectivity Proefschrift ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen op gezag van de rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties. De openbare verdediging zal plaatsvinden op 3 februari 2016 om uur door Mareike Karin Susanne van Duijl geboren op 18 september 1986 te Emden, Duitsland

5 Promotores Prof. J.M. Smit Prof. A.M. van Oijen Beoordelingscommissie Prof. E.J. Snijder Prof. F.M. Reggiori Prof. S.P.J. Whelan

6 Paranimfen Marie-Nicole Stephanie van de Wall Mariana Ruiz Silva

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8 Contents Chapter 1 General Introduction and Scope of the Thesis 9 Chapter 2 Dengue Virus Life Cycle and Pathogenesis 17 Chapter 3 Immature Dengue Virus Is Infectious in Human Immature Dendritic Cells via Interaction with the Receptor Molecule DC-SIGN PLoS ONE, 2014, 9(6): e Chapter 4 Early Events in Chikungunya Virus Infection From Virus Cell Binding to Membrane Fusion Viruses 2015, Chapter 5 Chapter 6 Chapter 7 Chapter 8 Dynamics of Chikungunya Virus Cell Entry Unraveled by Single Virus Tracking in Living Cells Submitted Chikungunya Virus Fusion Properties Elucidated by Single-Particle and Bulk Approaches Journal of General Virology (2015), 96, Development of a Highly Protective Combination Monoclonal Antibody Therapy against Chikungunya Virus PLoS Pathogens, 2013, 9(4): e Strongly Neutralizing Antibodies against Chikungunya Virus Inhibit Infection at Multiple Stages of the Viral Life Cycle Manuscript in preparation Chapter 9 Summarizing Discussion and Perspectives 189 Appendices Nederlandse samenvatting 225 Deutsche Zusammenfassung 233 Contributing authors 240 Curriculum vitae 241 Dankwoord 242

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