At a Glance. Bioengineering RALF Essentials. Bioengineering RALF Basic for Microbial Cultivation
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- Griffin Maxwell
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1 At a Glance Bioengineering RALF Essentials Bioengineering RALF Basic for Microbial Cultivation Single wall vessel with heating pad and cooling finger 1 gas channel, pulsed 2 fixed speed peristaltic pumps With agitation, temperature and ph control Incl. BioSCADA RALF for complete process automation and data acquisition, analysis and export Bioengineering RALF Basic for Cell Culture Single wall vessel with heating pad and cooling finger 3 gas channels, pulsed 2 fixed speed peristaltic pumps With agitation, temperature and ph control Incl. BioSCADA RALF for complete process automation and data acquisition, analysis and export Bioengineering RALF Advanced for Microbial Cultivation Single wall vessel with perfused baffles and heating circuit 1 gas channel, pulsed 2 fixed and 1 variable speed peristaltic pumps With agitation, temperature, ph, po2 and level / foam control Incl. BioSCADA RALF for complete process automation and data acquisition, analysis and export Bioengineering RALF Advanced for Cell Culture Double wall vessel with heating circuit 3 gas channels, pulsed 2 fixed and 1 variable speed peristaltic pumps With agitation, temperature, ph, po2 and level / foam control Incl. BioSCADA RALF for complete process automation and data acquisition, analysis and export 1
2 Configurations and Specifications Module Options Basic equipment Option Not possible Vessel Basic Advanced Single wall vessel Double wall vessel Baffles Direct drive with mechanical seal Drive, magnetically coupled Rushton impellers Marine impeller Pitched blade impeller Autoclavable benchtop fermenter Single wall glass vessel with rounded bottom Stainless steel lid with DN12 and DN04 ports Blind plugs DN12 Diaphragm port for inoculation Autoclavable benchtop fermenter Double wall glass vessel with rounded bottom Stainless steel lid with DN12 and DN04 ports Blind plugs DN12 Diaphragm port for inoculation Baffles, 4 pieces, stainless steel Direct drive from top by brushless DC motor, with mechanical seal, rpm controlled speed Direct drive from top by brushless DC motor, magnetically coupled, rpm controlled speed 2 six-blade Rushton type radial stirrers (diameter: see technical data) 1 marine impeller for axial mixing (diameter: see technical data) Pitched blade impeller, to be mounted on stirrer shaft, height-adjustable, for axial mixing (diameter: see technical data) 2 L 3.7 L 5 L 6.7 L 2 L 3.7 L 5 L 6.7 L Microbial Cell Microbial Cell Temperature Control Heating pad and cooling finger Heating circuit connected to perfused baffles Heating circuit connected to double wall of vessel Electrical heating jacket and cooling finger with solenoid valve for temperature control Heating and cooling by perfused stainless steel baffles connected to heating circuit with circulation pump, electrical heater and cooling water valve for temperature control Heating circuit with circulation pump, electrical heater and cooling water valve for temperature control
3 Aeration Basic Advanced Aeration tube Ring sparger Sinter sparger Primary gas module 1 gas, pulsed Primary gas module 3 gas, pulsed Primary gas module 1 gas, MFC Primary gas module 3 gas, with 2 pulsed valves and 1 MFC Primary gas module 3 gas, with 1 pulsed valve and 2 MFC Secondary gas module 1 gas, pulsed Secondary gas module 3 gas, pulsed Secondary gas module 1 gas, MFC Secondary gas module 3 gas, with 2 pulsed valves and 1 MFC Secondary gas module 3 gas, with 1 pulsed valve and 2 MFC Submerged aeration with sterile filter and check valve. The inlet gas is led to stirrer blades for efficient dispersion of bubbles. Submerged aeration with sterile filter and check valve. Aeration tube with ring sparger for efficient and careful submerged aeration. Submerged aeration with sterile filter and check valve. Aeration tube with sinter-metal microsparger for efficient submerged aeration also with low gas flow. One-channel gas supply unit. Pressure control valve, pressure gauge, rotameter for gas flow indication up to 2 vvm Air, needle valve and pulsed solenoid valve. (Other flow rates available, see technical data). Three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve and pulsed solenoid valve. (Other flow rates available, see technical data). One-channel gas supply unit. Pressure control valve, pressure gauge, rotameter for gas flow indication up to 2 vvm Air, needle valve and thermal mass flow controller up to 2 vvm Air. (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if secondary gas module does not have more than 1 mass flow controller). Three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve. 1st channel with thermal mass flow controller up to 0.1 vvm Air, 2nd and 3rd channel with pulsed solenoid valve (O2 and CO2). (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if secondary gas module does not have more than 1 mass flow controller). Three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve. 1st and 2nd channel with thermal mass flow controllers up to 0.1 vvm, 3rd channel with pulsed solenoid valve (CO2). (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if secondary gas module does not have a mass flow controller). Additional one-channel gas supply unit. Pressure control valve, pressure gauge, rotameter for gas flow indication up to 1 vvm O2 (microbial) or 0.1 vvm N2 (cell), needle valve and pulsed solenoid valve. (Other flow rates available, see technical data). Additional three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve and pulsed solenoid valve. (Other flow rates available, see technical data). Additional one-channel gas supply unit. Pressure control valve, pressure gauge, rotameter for gas flow indication up to 1 vvm O2 (microbial) or up to 0.1 vvm N2 (cell), needle valve and thermal mass flow controller up to 1 vvm O2 (microbial) or up to 0.1 vvm N2 (cell). (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if primary gas module does not have more than 1 mass flow controller). Additional three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve. 1st channel with thermal mass flow controller up to 0.1 vvm Air, 2nd and 3rd channel with pulsed solenoid valve (O2 and CO2). (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if primary gas module does not have more than 1 mass flow controller). Additional three-channel gas supply and mixing unit. Each channel with pressure control valve, pressure gauge, rotameter for gas flow indication up to 0.1 / 0.1 / 0.05 vvm Air / O2 / CO2, needle valve. 1st and 2nd channel with thermal mass flow controllers up to 0.1 vvm, 3rd channel with pulsed solenoid valve (CO2). (Other flow rates available, see technical data. Mass flow controller instead of pulsed valve. Only possible if primary gas module does not have a mass flow controller). 3 2 L 3.7 L 5L 6.7 L 2 L 3.7 L 5 L 6.7 L Microbial Cell Microbial Cell
4 Aeration Basic Advanced Secondary gas module for surface aeration Exhaust gas line, condenser Additional sterile filter, check valve and lid connection piece for independent surface aeration with secondary gas module. (For 2 L vessels not possible in combination with heating circuit via perfused baffles). Ventilation via water cooled glass condenser and subsequent sterile filter. Adjustable water flow. 2 L 3.7 L 5 L 6.7 L 2 L 3.7 L 5 L 6.7 L Microbial Cell Microbial Cell Addition / Transfer Immersion tube, height-adjustable Rotor filter package 20µm Primary pump module 2 x fixed speed Primary pump module 2 x fixed, 1 x variable speed Secondary pump module 2 x fixed speed Secondary pump module 2 x fixed, 1x variable speed Additional side pump 2 x bottle incl. cap and filter, with mounting bracket 3 x bottle incl. cap and filter, with mounting bracket Sampling system Height-adjustable immersion tube, stainless steel. DN06, for separate harvest. (For 2 L vessels not possible in combination with heating circuit via perfused baffles. Included in Rotorfilter package, not possible in addition). Open rotor filter with stainless steel sieve 20 µm pore size, mounted on stirrer shaft. Including level probe with controller, immersion tube and GL45 bottle cap with sterile filter for cell free harvest. (For 2 L vessels not possible in combination with heating circuit via perfused baffles). Pump module with 2 fixed speed peristaltic pumps, max. 130 rpm. 2 sets of hoses, 2 hose clamps. Pump module with 2 fixed speed and 1 variable speed peristaltic pumps, max. 130 rpm, reversible. 3 sets of hoses, 3 hose clamps. Additional pump module with 2 fixed speed peristaltic pumps, max. 130rpm. 2 sets of hoses, 2 hose clamps. Additional pump module with 2 fixed speed and 1 variable speed peristaltic pumps, max. 130 rpm, reversible. 3 sets of hoses, 3 hose clamps. Additional stand-alone peristaltic pump with local and remote speed display and control, rpm. 2 autoclavable glass bottles 250 ml, including GL45 screw cap with sterile filter and immersion tube, including mounting brackets to attach bottle to vessel. 3 autoclavable glass bottles 250 ml, each with GL45 screw cap and sterile filter, including mounting brackets to attach bottles to vessel. Sampling system with glass tube for hygienic sampling, including mounting bracket, including sampling tube stainless steel ID 3.5 mm. Autoclavable
5 Control Unit Basic Advanced Agitation speed control Temperature control ph control po2 control Foam / level control I / O package with additional interfaces and controllers BioSCADA RALF software package; installed, ready to use, if bought together with laptop Laptop Power supply: Europe, Asia Power supply: parts of America Measurement and control of agitation speed within rpm. Autoclavable Pt100 temperature probe and temperature controller activating actuators of heating / cooling system. Autoclavable ph probe and ph controller activating actuators (base and acid pump, CO2 valves). Autoclavable DO (po2) probe with DO controller, activating actuators and secondary controllers by configurable cascade control. Conductive level and foam probe with controller activating dosing or harvest pumps. (Can be used to control foam or level, not both together. Already included in rotor filter package, not possible in addition). Package consisting of: - 1 x RS232 input, connected to universal PID controller - 1 x 4 20 ma input, connected to universal PID controller - 1 x 4 20 ma input without controller - 4 x 4 20 ma outputs (from each universal controller, positive and negative) - 2 x digital outputs 24 V, freely configurable All inputs and outputs are available on sockets at the control cabinet housing One system for up to six fermenter units Runs with Windows system software. Without PC Customized main operation screen for system visualization and control Input of setpoints, process values, PID parameters for controllers Trends and historic process data visualization with scalable timeframe Display of calculated consumption rates of actuators Programming of cascades and profiles Calibration of in- and outputs Alarm management, event list Batch processing Programming of recipes; step sequence program Laptop PC with installed BioSCADA RALF 1 x 230 V, 50 / 60 Hz 1 x 110 V, 50 / 60 Hz 2 L 3.7 L 5L 6.7 L 2 L 3.7 L 5 L 6.7 L Microbial Cell Microbial Cell - - 5
6 Additional Options x Possible - Not possible a Only possible without heated perfused baffles (possible with double wall vessel). b Only possible without heated perfused baffles (possible with double wall vessel) and no other additional probe. c One additional probe possible, not in combination with another additional probe. Two additional probes possible in combination with double wall vessel without perfused baffles. Vessel Basic Advanced Rushton impeller, 6-blade, 40 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. x x x x x x x x Rushton impeller, 6-blade, 48 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. x x x x x x x x Rushton impeller, 6-blade, 60 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. - x x x - x x x Marine impeller, 4-blade, 48 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. x x x x x x x x Marine impeller, 4-blade, 66 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. - x x x - x x x Pitched blade impeller, 2-blade, 48 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. x x x x x x x x Pitched blade impeller, 2-blade, 66 mm outside diameter, for mounting on a 12 mm shaft. Stainless steel 316L. - x x x - x x x Temperature Control Basic Advanced Chiller 50 L for connection of up to six units. Including circulation pump. 230 V 50 / 60 Hz. x x x x x x x x Chiller 50 L for connection of up to six units. Including circulation pump. 110 V 50 / 60 Hz. x x x x x x x x Connection set for connecting of water supply and drain of up to 4 units to one connection point. x x x x x x x x Aeration Basic Advanced Aeration tube, 275 mm, for submerged aeration. The inlet gas is led to stirrer blades. x x x - x x x Aeration tube, 375 mm, for submerged aeration. The inlet gas is led to stirrer blades x x Aeration tube with ring sparger, 300 mm, small ring diameter, for submerged aeration. x x Aeration tube with ring sparger, 300 mm, large ring diameter, for submerged aeration. - x x - - x x Aeration tube with ring sparger, 400 mm, large ring diameter, for submerged aeration x x Aeration tube with sinter-metal microsparger for 2 L vessel. x x Aeration tube with sinter-metal microsparger for 3.7 L vessel. - x x Aeration tube with sinter-metal microsparger for 5 L vessel. - - x x Aeration tube with sinter-metal microsparger for 6.7 L vessel x x Surface aeration kit: Additional sterile filter, check valve, hoses and lid connection piece for independent surface aeration with a secondary gas module b x x x Addition / Transfer Basic Advanced Autoclavable glass bottle 250 ml, including GL45 screw cap with sterile filter and dip tube. x x x x x x x x Stainless steel mounting bracket to fix GL45 bottle with cap to the fermenter vessel unit. x x x x x x x x x screw cap for GL45 bottles, including gas filter. Autoclavable. x x x x x x x x Connection piece DN04, length 10 mm, for DN12 lid port. Stainless steel. x x x x x x x x Connection piece with DN12 diaphragm interface to connect hoses by needle-diaphragm technique. Including cup. Stainless steel. x x x x x x x x Cup DN12 to support diaphragms for needle connection to lid ports DN12. Stainless steel. x x x x x x x x Immersion tube Di 3.5 mm, length 245 mm, with connection piece for DN12 lid port. Stainless steel. x x x - x x x Immersion tube Di 3.5 mm, length 345 mm, with connection piece for DN12 lid port. Stainless steel x x Immersion tube Di 6 mm height-adjustable, for DN12 ports. Stainless steel, 355 mm. x x x x x x x x Immersion tube Di 6 mm height-adjustable, for DN12 ports. Stainless steel, 455 mm. x x x x x x x x Hypodermic needle Di 2 mm with cup for DN12 ports. Stainless steel. x x x x x x x x Hypodermic needle Di 3.5 mm with cup for DN12 ports. Stainless steel. x x x x x x x x Hypodermic needle Di 4 mm with cup for DN12 ports. Stainless steel. x x x x x x x x Sieve length 127 mm, pore size 10 µm. Stainless steel. x x x - x x x
7 Addition / Transfer Basic Advanced Rotorfilter to be mouted on stirrer shaft, height-adjustable. Sieve length 222 mm, pore size 10 µm. Stainless steel x x Sieve length 127 mm, pore size 20 µm. Stainless steel. x x x - x x x Sieve length 222 mm, pore size 20 µm. Stainless steel x x Sieve length 127 mm, pore size 30 µm. Stainless steel. x x x - x x x Sieve length 222 mm, pore size 30 µm. Stainless steel x x Sieve length 127 mm, pore size 40 µm. Stainless steel. x x x - x x x Sieve length 222 mm, pore size 40 µm. Stainless steel x x Sieve length 127 mm, pore size 75 µm. Stainless steel. x x x - x x x Sieve length 222 mm, pore size 75 µm. Stainless steel x x Sampling system with glass tube for hygienic sampling, including mounting bracket. Autoclavable. x x x x x x x x Control Unit Basic Advanced Gas analyzer type Exhalyzer to measure concentration of O2 (0 25%) and CO2 (0 10%). Incl. connection kit. EU power supply plug x x x x Gas analyzer type Exhalyzer to measure concentration of O2 (0 25%) and CO2 (0 10%). Incl. connection kit. US power supply plug x x x x Redox probe 256 mm with transmitter, incl ma cable. To be connected to existing universal controller of the Advanced model a c c Redox probe 311 mm with transmitter, incl ma cable. To be connected to existing universal controller of the Advanced model c Balance, range 10 35,000 g. Can be connected to the RS232 input of existing universal controller of the Advanced model x x x x Balance, range 1 16,100 g. Can be connected to the RS232 input of existing universal controller of the Advanced model x x x x Balance, range 1 6,500 g. Can be connected to the RS232 input of existing universal controller of the Advanced model x x x x Optical density probe 228 mm with controller box incl ma cable. To be connected to existing universal controller or analog input of the Advanced model a c c Optical density probe 283 mm with controller box incl ma cable. To be connected to existing universal controller or analog input of the Advanced model c
8 Upgrade Kits x Possible - Not possible d For Basic only possible for primary pump module. Side modules for gases and pumps that can be selected to upgrade or modify existing fermenter systems. Aeration Basic Advanced One-channel gas supply unit equipped with pressure control valve, pressure gauge, rotameter for gas flow indication up to 500L / h, needle valve and pulsed solenoid valve for gas flow control x x x x Same as above, but for up to 250 L / h x x x x Same as above, but for up to 100 L / h x x x x Same as above, but for up to 40 L / h x x x x Same as above, but for up to 16 L / h x x x x Same as above, but for up to 8 L / h x x x x Same as above, but for up to 5 L / h x x x x Three-channel gas supply unit, each channel equipped with pressure control valve, pressure gauge, rotameter for gas flow indication up to 100, 100 and 16 L / h, needle valve and pulsed solenoid valves for gas flow control x x x x Same as above, but for up to 40, 40 and 8 L / h x x x x Same as above, but for up to 16, 16, and 8 L / h x x x x Same as above, but for up to 8, 8 and 5 L / h x x x x Same as above, but for up to 5, 5 and 2 L / h x x x x One-channel gas supply unit, equipped with pressure control valve, pressure gauge, rotameter for gas flow indication up to 500L / h, needle valve and thermal mass flow controller up to 500 Ln / h x x x x Same as above, but for up to 250 L / h x x x x Same as above, but for up to 100 L / h x x x x Same as above, but for up to 40 L / h x x x x Same as above, but for up to 16 L / h x x x x Same as above, but for up to 8 L / h x x x x Same as above, but for up to 5 L / h x x x x Three-channel gas supply unit, each channel equipped with pressure control valve, pressure gauge, rotameter for gas flow indication up to 100, 100, and 16 L / h, needle valve. 1st channel with thermal mass flow controller up to 100 Ln / h, 2nd and 3rd channel with pulsed solenoid valve x x x x Same as above, but for up to 40, 40 and 8 L / h x x x x Same as above, but for up to 16, 16, and 8 L / h x x x x Same as above, but for up to 8, 8 and 5 L / h x x x x Same as above, but for up to 5, 5 and 2 L / h x x x x Three-channel gas supply unit, each channel equipped with pressure control valve, pressure gauge, rotameter for gas flow indication up to 100, 100, and 16 L / h, needle valve. 1st and 2nd channel with thermal mass flow controllers up to 100 Ln / h, 3rd channel with pulsed solenoid valve for gas flow control x x x x Same as above, but for up to 40, 40 and 8 L / h x x x x Same as above, but for up to 16, 16, and 8 L / h x x x x Same as above, but for up to 8, 8 and 5 L / h x x x x Same as above, but for up to 5, 5 and 2 L / h x x x x Addition / Transfer Basic Advanced Pump module with 2 fixed speed peristaltic pumps, 130 rpm. 2 sets of hoses, 2 hose clamps. x x x x x x x x Pump module with 2 fixed speed and 1 variable speed peristaltic pumps, max. 130 rpm, reversible. 3 sets of hoses, 3 hose clamps. d d d d x x x x
9 Vessel Kits Minimal selection Possible optional selection Not possible Spare vessel kit configurator for Bioengineering RALF. An additional autoclavable vessel unit - either single or double wall - for an existing RALF fermenter system can be chosen and configured with the table below. For the Basic model only a single wall vessel can be chosen. Single Wall Vessel Kit Basic Advanced Spare vessel kit single wall, autoclavable, minimal package. Including: glass vessel with lid and blind plugs, bearing and shaft, sampling / harvest tube, Pt100, condenser with hoses and connection pieces, inlet and outlet gas filter with hoses, check valve, cooling device (Basic: finger, Advanced: perfused baffles). Single wall vessel kit Single wall vessel kit Single wall vessel kit Single wall vessel kit Single wall vessel kit Single wall vessel kit Single wall vessel kit Single wall vessel kit Double Wall Vessel Kit Basic Advanced Spare vessel kit double wall, autoclavable, minimal package. Including: glass vessel with lid and blind plugs, bearing and shaft, sampling / harvest tube, Pt100, condenser with hoses and connection pieces, inlet and outlet gas filter with hoses, check valve. Double wall vessel kit Double wall vessel kit Double wall vessel kit Double wall vessel kit Additional Options to Vessel Kits Basic Advanced Baffles, 4 pieces (Advanced: only with double wall vessel) Baffles, 4 pieces (Advanced: only with double wall vessel) Baffles, 4 pieces (Advanced: only with double wall vessel) Baffles, 4 pieces (Advanced: only with double wall vessel) Six-blade Rushton type radial stirrer (ø 40 mm) (2 pcs. recommended for microbial culture) Six-blade Rushton type radial stirrer (ø 48 mm) (2 pcs. recommended for microbial culture) Six-blade Rushton type radial stirrer (ø 60 mm) (2 pcs. recommended for microbial culture) Marine impeller, for axial mixing (ø 48 mm) Marine impeller, for axial mixing (ø 66 mm) Pitched blade impeller, for axial mixing (ø 48 mm) Pitched blade impeller, for axial mixing (ø 66 mm) Aeration Items Basic Advanced Aeration tube Aeration tube Ring sparger Ring sparger Ring sparger Sinter-metal microsparger Sinter-metal microsparger Sinter-metal microsparger Sinter-metal microsparger
10 Addition / Transfer Items Basic Advanced Immersion tube, height-adjustable, short Immersion tube, height-adjustable, long Open rotor filter with stainless steel sieve 20 µm pore size, sieve length 127 mm, mounted on stirrer shaft. Immersion tube for cell free harvest. (For 2L vessels not possible in combination with heating circuit via perfused baffles. Availability of lid ports has to be checked) Open rotor filter with stainless steel sieve 20 µm pore size, sieve length 222 mm, mounted on stirrer shaft. Immersion tube for cell free harvest. (For 2L vessels not possible in combination with heating circuit via perfused baffles. Availability of lid ports has to be checked) Autoclavable glass bottle 250 ml, including GL45 screw cap with sterile filter and immersion tube, including hoses, including mounting brackets to attach bottle to vessel Sampling system with glass tube for hygienic sampling, including mounting bracket, autoclavable Control Unit Items Basic Advanced Autoclavable ph probe, short Autoclavable ph probe, long Autoclavable DO (po2) probe, length 300 mm. (In case of model Basic only functional if existing control system already includes transmitter and controller) Autoclavable DO (po2) probe, length 400 mm. (In case of model Basic only functional if existing control system already includes transmitter and controller) Conductive level and foam probe, length 254 mm. (In case of model Basic only functional if existing control system already includes transmitter and controller). (Can be used to control foam or level, not both together. Also necessary for level control together with rotor filter) Conductive level and foam probe, length 354 mm. (In case of model Basic only functional if existing control system already includes transmitter and controller). (Can be used to control foam or level, not both together. Also necessary for level control together with rotor filter)
11 Spare Parts x Available - Not available Small Set of Spare Parts Content: O-ring set, 2 x blind plug DN12, 1 x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin / HCl. Small set of spare parts x x Small set of spare parts - x x Small set of spare parts - - x x - - x x Large Set of Spare Parts Content: O-ring set, 2 x blind plug DN12, 1 x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin / HCl, po2 membrane kit, ph electrode, set of quick connections, condenser. Large set of spare parts x x Large set of spare parts - x x Large set of spare parts - - x x - - x x Set of Filters, Inlet and Outlet Gas Content: 4 x autoclavable filter 0.2 mm pore size, diameter 50 mm. Set of filters x x x x x x x x
12 Technical Data General 2 L 3.7 L 5 L 6.7 L Ambient temperature 5 40 C Relative humidity (non-condensing) 85% Operating temperature (cultivation) Max. 80 C Operating temperature (sterilization) Max. 130 C Operating pressure Max. 1.5 barg 21 psig Net weight [kg lbs], Basic1 unit Gross weight [kg lbs], Basic 1 unit wrapped Net weight [kg lbs], Advanced 1 unit Gross weight [kg lbs], Advanced 1 unit wrapped Weight autoclavable unit (empty) [kg lbs] Utility requirements Power supply CEE 7 / 7, 1 x 230 V, 50 / 60 Hz, 10 A fused NEMA 5 12, 1 x 110 V, 50 / 60 Hz, 16 A fused Max. power consumption (110 V) (230 V) Cooling water supply: connection flow pressure Hose nipples 6 / 1 mm 2 4 L / min bar ( psig) Cooling water return: connection flow pressure Hose nipples 6 / 1 mm 2 4 L / min pressureless Peak water consumption during cooling at 2 bar, with exhaust cooler Max. 250 L / h Average water consumption during cultivation mode Approx. 60 L / h Gas (dry, particle- and oil-free): connection flow pressure Pneumatic plug connection 8 / 1 mm L / h barg ( psig) Gas consumption Depending on process parameters Vessel 2 L 3.7 L 5 L 6.7 L Recommended working volume [L], max Recommended working volume [L], min Lid process connections DN04 connection tubes mm lid ports mm lid ports Stirrer port Motor type BLDC Motor torque [Nm] 1.6 Motor power [W] 230 Stirrer diameter, standard [mm] Rushton impeller ( 2 x ) Marine impeller ( 1 x ) Pitched blade impeller ( 1 x ) Material glass vessel (product contacted) Borosilicate Material steel parts (product contacted) 316L Steel parts surface roughness (product contacted) Ra < 0.8 µm Material polymer (product contacted) EPDM, PTFE, silicone 12
13 Temperature Control 2 L 3.7 L 5 L 6.7 L Temp. control range with cooling water (chilled) [ C] 4 80 Double jacketed vessel: electrical heater [W] 800 Single vessel: heating blankets [W] Heating-up time Approx. 1 min / C Cooling-down time Depending on cooling water temperature Requirements for external chiller Cooling water supply: connection pressure Cooling water return: connection pressure Cooling capacity up to 6 units Hose to nipples 6 / 1 mm bar ( psig) Hose to nipples 6 / 1 mm pressureless 600 W, 50 L water tank capacity Aeration 2 L 3.7 L 5 L 6.7 L Rotameter air for microbial cultivation * Ln / h Ln / h Ln / h Ln / h Rotameter air O2 CO2 for cell cultivation * Ln / h Ln / h Ln / h Ln / h Optional (2 in total, max.) Massflowmeter air for Advanced model microbial * Ln / h [±1.0%, 1:50] Ln / h [±1.0%, 1:50] Massflowmeter air O2 for Advanced model cell * Ln / h Ln / h Ln / h Ln / h [±1.0%, 1:50] [±1.0%, 1:50] [±1.0%, 1:50] [±1.0%, 1:50] In- and outlet filter * Other flow rates available: see upgrade kits 0.2 µm pore size Addition / Transfer 2 L 3.7 L 5 L 6.7 L Peristaltic pumps Pump head BioE Fix rpm 130 Var. rpm Flow rate hose Di 2.0 mm 35 ml / min Flow rate hose Di 3.5 mm 60 ml / min Storage bottles, volume [ml] 250 Control Unit 2 L 3.7 L 5 L 6.7 L Communication to PC / laptop RS485 / USB Temperature, Pt C ± 0.1 C Stirrer speed controlled rpm ±1 rpm ph, gel electrode 2 12 ± 0.05 ph po2, amperometric (Basic: optional) 6 ppb to saturation ± [1%+6 ppb] Foam and level, conductive on / off (Basic: optional) On / off, reaction time Free I / Os, Advanced only RS232 in 4 20 ma in 4 20 ma out 24 V out Minimum requirements for external PC / laptop Processor RAM HD optic. drive ports OS PIII, 1.2 GHz 512 MB 20 GB CD+ / -RW USB 2.0 Windows 2000, XP, 7 Screen Min. 15 color Material control tower Stainless steel AISI steel 37, varnished 13
14 F Hi A G H N L I Dimensions E D E M P Di K O C Q B R Front view Side view Top view A B C D E F G H I K L [mm] [mm] [mm] [mm] [mm] [mm] [mm] [mm] [mm] [mm] [mm] M* N O* P Q R Di Hi [mm] [mm] [mm] [mm] [mm] [mm] [mm] [mm] 2 L L L L A B C D E F G H I K L [inch] [inch] [inch] [inch] [inch] [inch] [inch] [inch] [inch] [inch] [inch] M* N O* P Q R Di Hi [inch] [inch] [inch] [inch] [inch] [inch] [inch] [inch] 2 L L L L * Max. dimensions for autoclaving 14
15 Arrangement type 1 Arrangement type 2 A C up to 6 units A D up to 6 units B C B D G (min.) G (min.) E (min.) F (min.) C D Top view (arrangement type 1) Top view (arrangement type 2) A B C D E* F* G* [mm] [mm] [mm] [mm] [mm] [mm] [mm] A B C D E* F* G* [inch] [inch] [inch] [inch] [inch] [inch] [inch] * Min. dimension for maintenance Technical data subject to change
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