Genetic Engineering Challenge How can scientists develop a type of rice that could prevent vitamin A deficiency? 1
|
|
- Daniela Greer
- 7 years ago
- Views:
Transcription
1 Genetic Engineering Challenge How can scientists develop a type of rice that could prevent vitamin A deficiency? 1 Vitamin A deficiency can result in blindness, severe infectious diseases, and even death, especially for children in poor countries. Vitamin A deficiency results when people do not have enough vitamin A or pro-vitamin A in their diets. Pro-vitamin A is a molecule that our bodies can easily convert to vitamin A. Some plant foods (e.g. carrots and sweet potatoes) are good sources of pro-vitamin A. Some animal foods (e.g. liver and eggs) are good sources of vitamin A. White rice is part of the rice seed. Brown rice includes white rice, the germ (which is the embryo that can grow into a new rice plant), and the surrounding bran layers. Rice is a good source of starch for energy and also provides some protein. However, neither white rice nor brown rice has pro-vitamin A. Therefore, many poor people in developing countries who eat mainly rice do not get enough pro-vitamin A or vitamin A in their diet. This results in many cases of blindness, severe infectious disease and/or death. 1. Why do rice plants include starch and protein in their seeds? What is the benefit for the rice plants? The germ is the embryo inside the seed. The bulk of the rice seed is the white rice which contains starch and storage proteins. For the rest of this activity, we will use the term rice grains to refer to white rice. No type of rice plant has been discovered that has pro-vitamin A in the rice grains; therefore, conventional breeding techniques cannot be used to develop a type of rice plant that has pro-vitamin A in the rice grains. So, some scientists who want to prevent vitamin A deficiency have used genetic engineering to develop rice plants that have rice grains with substantial pro-vitamin A. Genetic engineering is the process of manipulating genes in order to produce desired characteristics. In this activity we will discuss the type of genetic engineering that produces recombinant DNA (DNA which contains genes from two different organisms). We will learn how genetic engineering has been used to produce rice plants that have two additional genes inserted in their DNA, so these rice plants make substantial quantities of pro-vitamin A in their rice grains. 1 By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired), Teacher Preparation Notes, comments, and related activities are available at 1
2 2. Vitamin A and pro-vitamin A are molecules that contain only carbon and hydrogen atoms and do not have any amino acids. If scientists want to genetically engineer a plant to make provitamin A, what type of gene or genes would the scientists need to insert in the plant cells? Would they insert a gene that codes for pro-vitamin A? If not, what type of molecule would the gene or genes have to code for? 3. Scientists have identified genes for two enzymes needed to make pro-vitamin A. One of these genes comes from corn. If this gene from a corn plant is inserted in the DNA of a rice plant, will the sequence of amino acids in the protein produced by the rice plant be the same as the sequence of amino acids in the protein produced by the corn plant? In other words, will rice plants that have this gene produce the same enzyme as corn plants produce? Explain why or why not. You will learn about how scientists have genetically engineered rice plants to make pro-vitamin A in rice grains, but first answer question 4 to develop your own ideas about how this could be accomplished. 4a. Once scientists have identified the genes for enzymes to produce provitamin A, how could they insert these genes in the DNA of rice plant cells? Suggest one possibility. Be inventive! 4b. Would you recommend that scientists try to insert the genes for enzymes to produce provitamin A into: all the cells in a rice plant the thousands of cells in each rice grain or a small group of embryonic rice plant cells that can divide and develop into a rice plant? 2
3 Inserting the Desired Genes in the DNA of Rice Plants To insert genes from one organism into a different organism, scientists often take advantage of the natural genetic engineering capabilities of bacteria or viruses. One type of bacteria genetically engineers plant cells by inserting part of its bacterial DNA into the plant cell DNA, thus producing recombinant DNA. The inserted bacterial genes code for proteins that: stimulate the genetically engineered plant cells to produce food molecules that only the bacteria can use stimulate these genetically engineered plant cells to divide and form a growth that bulges out from the stem or root. 5. Explain how this type of genetic engineering is useful for the bacterium. This figure shows how the bacterium inserts some of its genes into the plant cell DNA. The plasmid is a small circle of DNA in the bacterium, separate from the chromosome. Only the T-DNA from the plasmid is inserted in the DNA in the nucleus of the plant cell. The genes in the T-DNA code for the proteins that result in the production of the food molecules for the bacteria and the proteins that result in increased cell division. The bacterial chromosome is shown at the bottom of the bacterium (A. tumifaciens). As in many diagrams, the relative dimensions are distorted; e.g., the bacterium appears much larger than it actually is relative to the plant cell and the plasmid appears much larger than it is relative to the bacterium. The plasmid is called the Ti or tumor inducing plasmid because the infected plant cells multiply and produce a bulging growth. The vir genes in the plasmid code for the proteins that carry out the transfer of the T-DNA from the bacteria into the plant cell DNA. (Figure from 6. In this example of genetic engineering in nature, where is the recombinant DNA found? This recombinant DNA contains DNA from the and the. 3
4 7. If a scientist wants to use the genetic engineering capabilities of these bacteria to carry the genes for the enzymes to make pro-vitamin A into a plant cell nucleus, where should she insert the genes for these enzymes? Use an arrow to indicate specifically where these genes should be inserted in the bacterium. The basic sequence of steps to use genetic engineering to produce rice plants that make provitamin A in rice grains is as follows: Scientists insert the genes for the enzymes to make pro-vitamin A in the bacterial plasmid. Bacteria with this modified plasmid are grown together with tissue culture rice plant cells, so the bacteria insert the genes for the enzymes to make pro-vitamin A in the DNA of the tissue culture rice plant cells. After the rice plant tissue culture cells have the genes for the enzymes to make pro-vitamin in their DNA, these genes are replicated together with the rest of the DNA before each cell division. These cells multiply and develop into a rice plant with the genes to make provitamin A in the DNA of each cell in the rice plant, including the thousands of cells in each rice grain. Ensuring that the Genes for the Enzymes to Make Pro-Vitamin A are Active in Rice Grain Cells 8. Almost all the cells in an animal or plant have exactly the same genes, but different types of cells make different types of proteins. This allows each type of cell to carry out its particular specialized function. Example 1: In humans, red blood cells have lots of, while muscle cells (contractile proteins or hemoglobin = O 2 -carrying protein) have lots of. (contractile proteins or hemoglobin) Example 2: Almost all the cells in a rice plant have exactly the same, (genes or proteins) but cells in the rice grains make storage proteins which provide nutrition for the developing embryo, whereas cells in the leaves make enzymes that make chlorophyll. 9. What is transcription? Why do cells need to carry out transcription of genes in order to make proteins? 4
5 10. Match each type of gene in the top list with the best match from the bottom list. Genes for enzymes to make chlorophyll Genes for storage proteins a. rate of transcription is higher in cells in rice grains b. rate of transcription is the same in cells in rice grains and cells in rice plant leaves c. rate of transcription is higher in cells in rice plant leaves For genetic engineering to be successful, scientists need to ensure that the genes they have inserted are transcribed, so the desired proteins are produced. Specifically, the scientists need to ensure that the genes for the enzymes to make pro-vitamin A are transcribed in the rice grain cells. Plant cells have a rather complex molecular mechanism to ensure the appropriate rate of transcription for each gene in each type of cell. A crucial part of this molecular mechanism is the promoter segment of DNA (located at the beginning of the gene). For example, the promoters for the genes for storage proteins promote high rates of transcription in the cells in rice grains. 11. Which promoter do you think would be a better promoter to insert at the beginning of the genes that code for the enzymes to produce pro-vitamin A? the promoter for a gene for an enzyme to make chlorophyll the promoter for a gene for a storage protein 12. The promoter is inserted in the bacterial plasmid at the beginning of the genes for the enzymes to produce provitamin A. Explain how the promoter and genes get from the plasmid in a bacterium into the DNA of a rice plant cell and then into the DNA of every cell in a rice plant. Plasmid Promoter + genes for enzymes 5
Name Class Date. Figure 13 1. 2. Which nucleotide in Figure 13 1 indicates the nucleic acid above is RNA? a. uracil c. cytosine b. guanine d.
13 Multiple Choice RNA and Protein Synthesis Chapter Test A Write the letter that best answers the question or completes the statement on the line provided. 1. Which of the following are found in both
More informationCCR Biology - Chapter 9 Practice Test - Summer 2012
Name: Class: Date: CCR Biology - Chapter 9 Practice Test - Summer 2012 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Genetic engineering is possible
More informationGENE CLONING AND RECOMBINANT DNA TECHNOLOGY
GENE CLONING AND RECOMBINANT DNA TECHNOLOGY What is recombinant DNA? DNA from 2 different sources (often from 2 different species) are combined together in vitro. Recombinant DNA forms the basis of cloning.
More informationRecombinant DNA and Biotechnology
Recombinant DNA and Biotechnology Chapter 18 Lecture Objectives What Is Recombinant DNA? How Are New Genes Inserted into Cells? What Sources of DNA Are Used in Cloning? What Other Tools Are Used to Study
More informationToday you will extract DNA from some of your cells and learn more about DNA. Extracting DNA from Your Cells
DNA Based on and adapted from the Genetic Science Learning Center s How to Extract DNA from Any Living Thing (http://learn.genetics.utah.edu/units/activities/extraction/) and BioRad s Genes in a bottle
More informationB2 1 Cells, Tissues and Organs
B2 Cells, Tissues and Organs 5 minutes 5 marks Page of 7 Q. The diagram shows a bacterium. On the drawing, name the structures labelled A, B, C and D. (Total 4 marks) Q2. (a) The diagrams show cells containing
More informationMitosis, Meiosis and Fertilization 1
Mitosis, Meiosis and Fertilization 1 I. Introduction When you fall and scrape the skin off your hands or knees, how does your body make new skin cells to replace the skin cells that were scraped off? How
More informationRespiration occurs in the mitochondria in cells.
B3 Question Which process occurs in the mitochondria in cells? Why do the liver and muscle cells have large number of mitochondria? What is the function of the ribosomes? Answer Respiration occurs in the
More informationNutrients: Carbohydrates, Proteins, and Fats. Chapter 5 Lesson 2
Nutrients: Carbohydrates, Proteins, and Fats Chapter 5 Lesson 2 Carbohydrates Definition- the starches and sugars found in foods. Carbohydrates are the body s preferred source of energy providing four
More informationWho took Jerell s ipod? -- An organic compound mystery 1
Who took Jerell s ipod? -- An organic compound mystery 1 Jerell is a 10 th grade student who works at McDonald s on the weekends. While on break, Jerell was studying for his biology test and listening
More informationGenetics 1. Defective enzyme that does not make melanin. Very pale skin and hair color (albino)
Genetics 1 We all know that children tend to resemble their parents. Parents and their children tend to have similar appearance because children inherit genes from their parents and these genes influence
More informationReproductive System & Development: Practice Questions #1
Reproductive System & Development: Practice Questions #1 1. Which two glands in the diagram produce gametes? A. glands A and B B. glands B and E C. glands C and F D. glands E and F 2. Base your answer
More information1 Mutation and Genetic Change
CHAPTER 14 1 Mutation and Genetic Change SECTION Genes in Action KEY IDEAS As you read this section, keep these questions in mind: What is the origin of genetic differences among organisms? What kinds
More informationEndocrine System: Practice Questions #1
Endocrine System: Practice Questions #1 1. Removing part of gland D would most likely result in A. a decrease in the secretions of other glands B. a decrease in the blood calcium level C. an increase in
More informationCellular Respiration: Practice Questions #1
Cellular Respiration: Practice Questions #1 1. Which statement best describes one of the events taking place in the chemical reaction? A. Energy is being stored as a result of aerobic respiration. B. Fermentation
More informationCells & Cell Organelles
Cells & Cell Organelles The Building Blocks of Life H Biology Types of cells bacteria cells Prokaryote - no organelles Eukaryotes - organelles animal cells plant cells Cell size comparison Animal cell
More informationName: Date: Period: DNA Unit: DNA Webquest
Name: Date: Period: DNA Unit: DNA Webquest Part 1 History, DNA Structure, DNA Replication DNA History http://www.dnaftb.org/dnaftb/1/concept/index.html Read the text and answer the following questions.
More informationClass Time: 30 minutes. Other activities in the Stem Cells in the Spotlight module can be found at: http://gslc.genetics.utah.edu/teachers/tindex/
Teacher Guide: Color-Label-Learn: Creating Stem Cells for Research ACTIVITY OVERVIEW Abstract: Students color and label images on a worksheet and answer questions about the on-line content featured in
More informationGenetics Test Biology I
Genetics Test Biology I Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Avery s experiments showed that bacteria are transformed by a. RNA. c. proteins.
More informationHUMAN PROTEINS FROM GENETIC ENGINEERING OF ORGANISMS
HUMAN PROTEINS FROM GM BACTERIA Injecting insulin is an everyday event for many people with diabetes. GENETIC ENGINEERING OF ORGANISMS involves transferring genes from one species into another. Genetic
More information1. The diagram below represents a biological process
1. The diagram below represents a biological process 5. The chart below indicates the elements contained in four different molecules and the number of atoms of each element in those molecules. Which set
More informationPRESTWICK ACADEMY NATIONAL 5 BIOLOGY CELL BIOLOGY SUMMARY
Name PRESTWICK ACADEMY NATIONAL 5 BIOLOGY CELL BIOLOGY SUMMARY Cell Structure Identify animal, plant, fungal and bacterial cell ultrastructure and know the structures functions. Plant cell Animal cell
More informationStructure and Function of DNA
Structure and Function of DNA DNA and RNA Structure DNA and RNA are nucleic acids. They consist of chemical units called nucleotides. The nucleotides are joined by a sugar-phosphate backbone. The four
More informationCells, tissues and organs
Chapter 8: Cells, tissues and organs Cells: building blocks of life Living things are made of cells. Many of the chemical reactions that keep organisms alive (metabolic functions) take place in cells.
More informationCell and Membrane Practice. A. chromosome B. gene C. mitochondrion D. vacuole
Name: ate: 1. Which structure is outside the nucleus of a cell and contains N?. chromosome. gene. mitochondrion. vacuole 2. potato core was placed in a beaker of water as shown in the figure below. Which
More informationDNA Fingerprinting. Unless they are identical twins, individuals have unique DNA
DNA Fingerprinting Unless they are identical twins, individuals have unique DNA DNA fingerprinting The name used for the unambiguous identifying technique that takes advantage of differences in DNA sequence
More informationThe Molecules of Cells
The Molecules of Cells I. Introduction A. Most of the world s population cannot digest milk-based foods. 1. These people are lactose intolerant because they lack the enzyme lactase. 2. This illustrates
More informationGiven these characteristics of life, which of the following objects is considered a living organism? W. X. Y. Z.
Cell Structure and Organization 1. All living things must possess certain characteristics. They are all composed of one or more cells. They can grow, reproduce, and pass their genes on to their offspring.
More informationGenetics Module B, Anchor 3
Genetics Module B, Anchor 3 Key Concepts: - An individual s characteristics are determines by factors that are passed from one parental generation to the next. - During gamete formation, the alleles for
More informationDraw one line from each structure in List A to the correct information about the structure in List B.
Q. The drawing shows the cell of a bacterium. (a) List A gives the four structures labelled on the diagram. List B includes information about each structure. Draw one line from each structure in List A
More informationBio EOC Topics for Cell Reproduction: Bio EOC Questions for Cell Reproduction:
Bio EOC Topics for Cell Reproduction: Asexual vs. sexual reproduction Mitosis steps, diagrams, purpose o Interphase, Prophase, Metaphase, Anaphase, Telophase, Cytokinesis Meiosis steps, diagrams, purpose
More information12.1 The Role of DNA in Heredity
12.1 The Role of DNA in Heredity Only in the last 50 years have scientists understood the role of DNA in heredity. That understanding began with the discovery of DNA s structure. In 1952, Rosalind Franklin
More informationGene Switches Teacher Information
STO-143 Gene Switches Teacher Information Summary Kit contains How do bacteria turn on and turn off genes? Students model the action of the lac operon that regulates the expression of genes essential for
More informationUnit I: Introduction To Scientific Processes
Unit I: Introduction To Scientific Processes This unit is an introduction to the scientific process. This unit consists of a laboratory exercise where students go through the QPOE2 process step by step
More informationa mutation that occurs during meiosis results in a chromosomal abnormality B.
Biotechnology 1. Which of the following is an example of gene splicing? a segment of human DNA is inserted into the DNA sequence of a bacterium a mutation that occurs during meiosis results in a chromosomal
More information3120-1 - Page 1. Name:
Name: 1) Which series is arranged in correct order according to decreasing size of structures? A) DNA, nucleus, chromosome, nucleotide, nitrogenous base B) chromosome, nucleus, nitrogenous base, nucleotide,
More informationBiotechnology: DNA Technology & Genomics
Chapter 20. Biotechnology: DNA Technology & Genomics 2003-2004 The BIG Questions How can we use our knowledge of DNA to: diagnose disease or defect? cure disease or defect? change/improve organisms? What
More informationDNA Scissors: Introduction to Restriction Enzymes
DNA Scissors: Introduction to Restriction Enzymes Objectives At the end of this activity, students should be able to 1. Describe a typical restriction site as a 4- or 6-base- pair palindrome; 2. Describe
More informationB2 Revision. Subject Module Date Biology B2 13 TH May (am)
B2 Revision Subject Module Date Biology B2 13 TH May (am) Useful websites www.aqa.org.uk This website contains the specifications that we follow and also has a large number of past papers and mark schemes
More informationFOR TEACHERS ONLY. The University of the State of New York REGENTS HIGH SCHOOL EXAMINATION LIVING ENVIRONMENT
FOR TEACHERS ONLY LE The University of the State of New York REGENTS HIGH SCHOOL EXAMINATION LIVING ENVIRONMENT Tuesday, June 21, 2011 9:15 a.m. to 12:15 p.m., only SCORING KEY AND RATING GUIDE Directions
More informationOrganelle Speed Dating Game Instructions and answers for teachers
Organelle Speed Dating Game Instructions and answers for teachers These instructions should accompany the OCR resources GCSE (9 1) Combined Science 21 st Century Science B Organelle Speed Dating Game learner
More informationBiopharmaceuticals and Biotechnology Unit 2 Student Handout. DNA Biotechnology and Enzymes
DNA Biotechnology and Enzymes 35 Background Unit 2~ Lesson 1 The Biotechnology Industry Biotechnology is a process (or a technology) that is used to create products like medicines by using micro-organisms,
More informationImmunology Ambassador Guide (updated 2014)
Immunology Ambassador Guide (updated 2014) Immunity and Disease We will talk today about the immune system and how it protects us from disease. Also, we ll learn some unique ways that our immune system
More informationCystic Fibrosis Webquest Sarah Follenweider, The English High School 2009 Summer Research Internship Program
Cystic Fibrosis Webquest Sarah Follenweider, The English High School 2009 Summer Research Internship Program Introduction: Cystic fibrosis (CF) is an inherited chronic disease that affects the lungs and
More informationModule 3 Questions. 7. Chemotaxis is an example of signal transduction. Explain, with the use of diagrams.
Module 3 Questions Section 1. Essay and Short Answers. Use diagrams wherever possible 1. With the use of a diagram, provide an overview of the general regulation strategies available to a bacterial cell.
More informationMs. Campbell Protein Synthesis Practice Questions Regents L.E.
Name Student # Ms. Campbell Protein Synthesis Practice Questions Regents L.E. 1. A sequence of three nitrogenous bases in a messenger-rna molecule is known as a 1) codon 2) gene 3) polypeptide 4) nucleotide
More informationBasic Concepts Recombinant DNA Use with Chapter 13, Section 13.2
Name Date lass Master 19 Basic oncepts Recombinant DN Use with hapter, Section.2 Formation of Recombinant DN ut leavage Splicing opyright lencoe/mcraw-hill, a division of he Mcraw-Hill ompanies, Inc. Bacterial
More informationControl of Gene Expression
Control of Gene Expression (Learning Objectives) Explain the role of gene expression is differentiation of function of cells which leads to the emergence of different tissues, organs, and organ systems
More informationDetermination of Specific Nutrients in Various Foods. Abstract. Humans need to consume food compounds such as carbohydrates, proteins, fats,
Determination of Specific Nutrients in Various Foods Abstract Humans need to consume food compounds such as carbohydrates, proteins, fats, and vitamins to meet their energy requirements. In this lab, reagents
More information13.4 Gene Regulation and Expression
13.4 Gene Regulation and Expression Lesson Objectives Describe gene regulation in prokaryotes. Explain how most eukaryotic genes are regulated. Relate gene regulation to development in multicellular organisms.
More informationGenetics 301 Sample Final Examination Spring 2003
Genetics 301 Sample Final Examination Spring 2003 50 Multiple Choice Questions-(Choose the best answer) 1. A cross between two true breeding lines one with dark blue flowers and one with bright white flowers
More informationAnimal Pharming: The Industrialization of Transgenic Animals December 1999
Animal Pharming: The Industrialization of Transgenic Animals December 1999 Animal pharming, the process of using transgenic animals to produce human drugs, is staking its claim in a lucrative world market.
More informationChapter 23 Definitions of GMO/LMO and modern biotechnology. Three different definitions but the same legal interpretation?
Chapter 23 Definitions of GMO/LMO and modern biotechnology JAN HUSBY NORWEGIAN INSTITUTE OF GENE ECOLOGY (GENØK), TROMSØ, NORWAY Three different definitions but the same legal interpretation? There are
More informationGenetic Engineering and Biotechnology
1 So, what is biotechnology?? The use of living organisms to carry out defined chemical processes for industrial or commercial application. The office of Technology Assessment of the U.S. Congress defines
More information7.2 Cells: A Look Inside
CHAPTER 7 CELL STRUCTURE AND FUNCTION 7.2 Cells: A Look Inside Imagine a factory that makes thousands of cookies a day. Ingredients come into the factory, get mixed and baked, then the cookies are packaged.
More information7.1 What Are Cells? You are made of cells. A cell is the basic unit of structure and function in a living thing. CHAPTER 7
CELL STRUCTURE AND FUNCTION 7.1 What Are Cells? Look closely at the skin on your arm. Can you see that it is made of cells? Of course not! Your skin cells are much too small to see with your eyes. Now
More informationControl of Gene Expression
Home Gene Regulation Is Necessary? Control of Gene Expression By switching genes off when they are not needed, cells can prevent resources from being wasted. There should be natural selection favoring
More informationRNA & Protein Synthesis
RNA & Protein Synthesis Genes send messages to cellular machinery RNA Plays a major role in process Process has three phases (Genetic) Transcription (Genetic) Translation Protein Synthesis RNA Synthesis
More informationThe Huntington Library, Art Collections, and Botanical Gardens. How Sweet It Is: Enzyme Action in Seed Germination
The Huntington Library, Art Collections, and Botanical Gardens How Sweet It Is: Enzyme Action in Seed Germination Overview This experiment is intended to familiarize students with the macromolecule starch,
More informationTopic 3: Nutrition, Photosynthesis, and Respiration
1. Base your answer to the following question on the chemical reaction represented below and on your knowledge of biology. If this reaction takes place in an organism that requires sunlight to produce
More informationEnzymes. A. a lipid B. a protein C. a carbohydrate D. a mineral
Enzymes 1. All cells in multicellular organisms contain thousands of different kinds of enzymes that are specialized to catalyze different chemical reactions. Given this information, which of the following
More informationName Date Period. Keystone Review Enzymes
Name Date Period Keystone Review Enzymes 1. In order for cells to function properly, the enzymes that they contain must also function properly. What can be inferred using the above information? A. Cells
More informationName Date Period. 2. When a molecule of double-stranded DNA undergoes replication, it results in
DNA, RNA, Protein Synthesis Keystone 1. During the process shown above, the two strands of one DNA molecule are unwound. Then, DNA polymerases add complementary nucleotides to each strand which results
More informationGet It Right. Answers. Chapter 1: The Science of Life. A biologist studies all living things.
Discover Biology 'N' Level Science Chapter 1 Chapter 1: The Science of Life A biologist studies all living things. In order to carry out the scientific method, we need to ask questions. Discover Biology
More informationNutritional Glossary. Index of Contents
Nutritional Glossary This glossary provides nutrition information about the nutrients commonly found in fruits, vegetables, and other plant foods Each glossary definition has a long and a short version.
More informationChapter 3. Cellular Structure and Function Worksheets. 39 www.ck12.org
Chapter 3 Cellular Structure and Function Worksheets (Opening image copyright by Sebastian Kaulitzki, 2010. Used under license from Shutterstock.com.) Lesson 3.1: Introduction to Cells Lesson 3.2: Cell
More informationDNA Technology Mapping a plasmid digesting How do restriction enzymes work?
DNA Technology Mapping a plasmid A first step in working with DNA is mapping the DNA molecule. One way to do this is to use restriction enzymes (restriction endonucleases) that are naturally found in bacteria
More informationTopic 4: Digestion and Nutrition
Topic 4: Digestion and Nutrition THE CONTENTS OF FOOD Food contains nutrients: Nutrients include: 1. 2. 3. 4. 5. Nutrients must be small enough to enter our cells. If they are too large they must be digested
More informationIntroduction. Introduction Nutritional Requirements. Six Major Classes of Nutrients. Water 12/1/2011. Regional Hay School -- Bolivar, MO 1
Cattle and Horse Nutrition Dona Goede Livestock Specialist Introduction Many health, reproductive and production problems can be prevented with good nutrition. Poor nutrition results in: Poor conception
More informationCancer Genomics: What Does It Mean for You?
Cancer Genomics: What Does It Mean for You? The Connection Between Cancer and DNA One person dies from cancer each minute in the United States. That s 1,500 deaths each day. As the population ages, this
More informationGreen Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein
Green Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein INTRODUCTION Green Fluorescent Protein (GFP) is a novel protein produced by the bioluminescent
More informationAP BIOLOGY 2010 SCORING GUIDELINES (Form B)
AP BIOLOGY 2010 SCORING GUIDELINES (Form B) Question 2 Certain human genetic conditions, such as sickle cell anemia, result from single base-pair mutations in DNA. (a) Explain how a single base-pair mutation
More informationMultiple Choice Questions
Chapter 5 THE FUNDAMENTAL UNIT OF LIFE Multiple Choice Questions 1. Which of the following can be made into crystal? (a) A Bacterium (b) An Amoeba (c) A Virus (d) A Sperm 2. A cell will swell up if (a)
More informationModule 3: Strawberry DNA Extraction
Module 3: Strawberry DNA Extraction Teacher/Leader Target Audience: 7-12 Life Science, Biology, Ag Science Overview: In this lab, students will extract DNA from a strawberry using everyday materials and
More informationThe Immune System and Disease
Chapter 40 The Immune System and Disease Section 40 1 Infectious Disease (pages 1029 1033) This section describes the causes of disease and explains how infectious diseases are transmitted Introduction
More informationTransfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.
Transfection Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide,
More informationDifferentiation = Making specialized cells. Your body needs stem cells. What is a stem cell?
Differentiation = Making specialized cells What is a stem cell? What the photo shows A lump of metal and lots of different kinds of screws. Things to think about Lumps of metal that start off the same
More informationGenetics Lecture Notes 7.03 2005. Lectures 1 2
Genetics Lecture Notes 7.03 2005 Lectures 1 2 Lecture 1 We will begin this course with the question: What is a gene? This question will take us four lectures to answer because there are actually several
More informationDNA Replication & Protein Synthesis. This isn t a baaaaaaaddd chapter!!!
DNA Replication & Protein Synthesis This isn t a baaaaaaaddd chapter!!! The Discovery of DNA s Structure Watson and Crick s discovery of DNA s structure was based on almost fifty years of research by other
More informationNUTRIENTS: THEIR INTERACTIONS
NUTRIENTS: THEIR INTERACTIONS TEACHER S GUIDE INTRODUCTION This Teacher s Guide provides information to help you get the most out of Nutrients: Their Interactions. The contents in this guide will allow
More informationrestriction enzymes 350 Home R. Ward: Spring 2001
restriction enzymes 350 Home Restriction Enzymes (endonucleases): molecular scissors that cut DNA Properties of widely used Type II restriction enzymes: recognize a single sequence of bases in dsdna, usually
More informationLesson 13 Genetic modification
77 Lesson 13 modification 78 modification Suitable for: 14 16 years Curriculum and learning links: modification Learning objectives: Describe the process of genetic modification. Explain some of the ethical
More informationThe E. coli Insulin Factory
The E. coli Insulin Factory BACKGROUND Bacteria have not only their normal DNA, they also have pieces of circular DNA called plasmids. Plasmids are a wonderfully ally for biologists who desire to get bacteria
More informationBacteria vs. Virus: What s the Difference? Grade 11-12
Bacteria vs. Virus: What s the Difference? Grade 11-12 Subject: Biology Topic: Bacteria, viruses, and the differences between them. The role that water plays in spreading bacteria and viruses, and the
More informationTranslation Study Guide
Translation Study Guide This study guide is a written version of the material you have seen presented in the replication unit. In translation, the cell uses the genetic information contained in mrna to
More informationSubject Area(s) Biology. Associated Unit Engineering Nature: DNA Visualization and Manipulation. Associated Lesson Imaging the DNA Structure
Subject Area(s) Biology Associated Unit Engineering Nature: DNA Visualization and Manipulation Associated Lesson Imaging the DNA Structure Activity Title Inside the DNA Header Image 1 ADA Description:
More informationMutations: 2 general ways to alter DNA. Mutations. What is a mutation? Mutations are rare. Changes in a single DNA base. Change a single DNA base
Mutations Mutations: 2 general ways to alter DNA Change a single DNA base Or entire sections of DNA can move from one place to another What is a mutation? Any change in the nucleotide sequence of DNA Here
More informationRegents Biology REGENTS REVIEW: PROTEIN SYNTHESIS
Period Date REGENTS REVIEW: PROTEIN SYNTHESIS 1. The diagram at the right represents a portion of a type of organic molecule present in the cells of organisms. What will most likely happen if there is
More informationStem Cells. Part 1: What is a Stem Cell?
Stem Cells Part 1: What is a Stem Cell? Stem cells differ from other kinds of cells in the body. When a stem cell divides by mitosis, each new cell has the potential to either remain a stem cell or become
More informationAP BIOLOGY 2007 SCORING GUIDELINES
AP BIOLOGY 2007 SCORING GUIDELINES Question 4 A bacterial plasmid is 100 kb in length. The plasmid DNA was digested to completion with two restriction enzymes in three separate treatments: EcoRI, HaeIII,
More informationBlue Print III Biology. Class XII. Genetics and Evolution 2 (2) 4 (2) 9 (3) 5 (1) 20 (8) Types of Questions VSA SA II SA I LA Total
Blue Print III Biology Class XII Types of Questions VSA SA II SA I LA Total Units (1 mark) ( marks) (3 marks) (5 marks) - Sexual Reproduction () 4 () 6 () _ 1 (6) Genetics and Evolution () 4 () 9 (3) 5
More informationControl of Gene Expression
Control of Gene Expression What is Gene Expression? Gene expression is the process by which informa9on from a gene is used in the synthesis of a func9onal gene product. What is Gene Expression? Figure
More informationActivity 7.21 Transcription factors
Purpose To consolidate understanding of protein synthesis. To explain the role of transcription factors and hormones in switching genes on and off. Play the transcription initiation complex game Regulation
More informationChapter 18 Regulation of Gene Expression
Chapter 18 Regulation of Gene Expression 18.1. Gene Regulation Is Necessary By switching genes off when they are not needed, cells can prevent resources from being wasted. There should be natural selection
More informationAn Overview of Cells and Cell Research
An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism
More informationCells in Biology. Lesson 1.
Lesson 1. Cells in Biology. Jump-Start Your Learning. Before you begin reading, take a piece of paper and write ''Cells'' across the top. Then, as fast as you can, jot down any notes, facts, opinions or
More informationPHOTOSYNTHESIS AND CELLULAR RESPIRATION
reflect Wind turbines shown in the photo on the right are large structures with blades that move in response to air movement. When the wind blows, the blades rotate. This motion generates energy that is
More informationCell Unit Practice Test #1
ell Unit Practice Test #1 Name: ate: 1. Which organelle is primarily concerned with the conversion of potential energy of organic compounds into suitable form for immediate use by the cell?. mitochondria.
More informationCHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA
CHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA INTRODUCTION DNA : DNA is deoxyribose nucleic acid. It is made up of a base consisting of sugar, phosphate and one nitrogen base.the
More informationChapter 3 Molecules of Cells
Bio 100 Molecules of cells 1 Chapter 3 Molecules of Cells Compounds containing carbon are called organic compounds Molecules such as methane that are only composed of carbon and hydrogen are called hydrocarbons
More information