Catarina Brito. Curriculum Vitae October 2012

Transcription

1 Catarina Brito Curriculum Vitae October 2012 PERSONAL DATA Name: Ana Catarina Maurício Brito Ataíde Montes Date of birth: September 8, 1976 Nationality: Portuguese Institutional Address: Animal Cell Technology Unit, ITQB UNL & ibet, Apartado 12, P Oeiras, Portugal Tel:(+351) ; Fax: (+351) anabrito@itqb.unl.pt web page: ResearcherID: A EDUCATION Diploma in Biochemistry, Faculdade de Ciências da Universidade de Lisboa, Portugal, October Ph.D. in Biochemistry, Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Portugal, October SCIENTIFIC ACTIVITY Previous and Current Positions : Undergraduate student at the Glycobiology Laboratory, ITQB, Portugal : Graduate research fellow at Laboratory of Glycobiology, ITQB, Portugal : Ph.D. student at Laboratory of Glycobiology, ITQB, Portugal. 2006: Visiting Ph.D. student in the Membrane Traffic in Neuronal & Epithelial Morphogenesis Laboratory, at Institut Jacques Monod, Paris, France : Post doctoral fellow at Animal Cell Technology Unit, ibet, Portugal. Since July 2009: Research Associate Investigador Auxiliar Ciência 2008, Head of the Bioassay Development Laboratory, Animal Cell Technology Unit, Instituto de Biologia Experimental e Tecnológica (ibet) /ITQB UNL, Portugal. Research Interests My major research goal, with funding from Fundação para a Ciência e Tecnologia (Portugal), the Innovative Medicine Initiative (IMI) EU EFPIA program and the Pharmaceutical Industry, is to integrate bioprocessing development and molecular cell biology techniques to deliver novel and validated 3D in vitro models for preclinical research, using stem cells and patient derived cells. Currently, our research targets are Cancer (solid tumors), Central Nervous System diseases, neural and hepatic toxicology. Complementary to this, I have been providing contract research services to the pharmaceutical and biotechnology industry. As project manager, I have been focusing in bridging my own competences with the ones of the existing R&D and infrastructural units of IBET to provide integrated solutions in the areas of drug development and pre clinical research. Keywords: Cellular Biotechnology and Stem Cell Bioengineering; 3D in vitro models. Other research interests: Intracellular trafficking; Recombinant protein expression; Post translation modifications. 1/6

2 Participation in Funded Projects (FCT and EU) As Principal Investigator (Since 2009): : 3D in vitro models for reducing animal experimentation in pharmaceutical development: integrative approaches for prediction of hepatic drug metabolism and neurotoxicity, PTDC/EEB BIO/112786/2009, funded by FCT, Portugal : PREDECT New Models for preclinical evaluation of drug efficacy in common solid tumors, funded by Innovative Medicines Initiative Joint Undertaking (EU & European Federation of Pharmaceutical Industries and Associations) : Human central nervous system in vitro models for preclinical research: new tools for studying viral vector mediated gene delivery in a 3D cellular context, PTDC/EBB BIO/119243/2010, funded by FCT, Portugal. As Team Member: : Glicosilação das mucinas na mucosa gástrica normal, carcinoma gástrico e linhas celulares de carcinoma gástrico: papel da glicosilação e das apomucinas PRAXIS/SAU/14111/1998, funded by FCT, Portugal : Identificação e caracterização de proteínas envolvidas na organização do Golgi de células de mamífero POCTI/BCI/38631/99, funded by FCT, Portugal : Clinical, Neurophysiological and Neurochemical studies in Amyotrophic Lateral Sclerosis", POCTI/CBO/43952/2002, funded by FCT, Portugal : STREP Signaling and Membrane Trafficking in Transformation and Differentiation, LSHG CT , funded by 6 th Framework Programme, EU : CellPROM Cell Programming by Nanoscaled Devices, NMP4 CT , funded by 6 th Framework Programme, EU : CLINIGENE European Network for the Advancement of Clinical Gene Transfer and Therapy, LSHB CT , funded by 6 th Framework Programme, EU : BrainCAV Nonhuman adenovirus vectors for gene to the Brain, HEALTH F , funded by 7 th Framework Programme, EU : Integrated strategy for expansion, neuronal differentiation and cryopreservation of human embryonic stem cells, PTDC/BIO/72755/2006, funded by FCT, Portugal : Hyperlab High Yield and Performance Stem Cell Lab, funded by 7 th Framework Programme, EU : 2D fluorometry: a powerful tool to improve mammalian cell process development, PTDC/EBB EBI/102750/2008, funded by FCT, Portugal : BACULOME Engineering cellular energetics for improvement of bioprocesses: metabolic modeling for enhanced vaccines production, PTDC/EBB EBI/103359/2008, funded by FCT, Portugal : CAREMI Cardio Repair European Multidisciplinary Initiative, HEALTH F , funded by 7 th Framework Programme, EU : SpeCCC Specialised Cell Carrier Components, FP7 SME SpeCCC, funded by 7 th Framework Programme, EU. 2/6

3 : Transbio Sudoe The southern European transnational cluster SOE3/P1/F498, funded by Interreg IVB, EU. Participation in Funded Projects (with Industry and Companies) As Project Manager (Since 2009): : Micromet, Germany, Development of cell based assays for assessment of cell mediated cytotoxicity induced by cancer drugs candidates : Tecnimede, Portugal, Neuronal cell models for pharmacological development : Tecnimede, Portugal, Development of complex neural in vitro models for pharmacological development. As Team Member: : CEVEC, Germany, Novel expression system for the production of human glycoproteins : Centocor, J&J, USA, Development of bioprocesses for Stem Cells for Cell Therapy applications : MERCK, Germany, Novel cell lines based on lentiviral vector technology. Fellowships and Awards 2001: Top Biochemistry degree thesis (final mark of 20/20), Faculdade de Ciências da Universidade de Lisboa : Fellowship for Initiation to Science (BIC), ibet, Oeiras, Portugal. 2002: Research Fellowship (BI), ITQB, Oeiras, Portugal : Ph.D. Fellowship, Fundação para a Ciência e Tecnologia, Portugal. 2004: FEBS Youth Travel Fund Award for participation in the EMBO/FEBS Practical Course Electron Microscopy and Stereology in Cell Biology : Post doctoral Fellowship, Fundação para a Ciência e Tecnologia, Portugal. 2012: EMBL Fellowship for participation in the EMBL Conference Stem Cells in Cancer and Regenerative Medicine. Professional Societies Member of: Federation of European Biochemical Societies (FEBS) European Society for Animal Cell Technology (ESACT), Portuguese Biochemistry Society (SPB), Portuguese Society of Biotechnology (SPBT), Portuguese Society for Stem Cells and Cell Therapy (SPCE TC). ACADEMIC ACTIVITY Supervision Activity Participation in the training and supervision of master and PhD students (Since 2005): Armanda Rodrigues, Marcos Sousa, Marta Silva; Eda Machado, Cristina Escrevente, Ricardo Gouveia, Catarina Gomes, António Roldão, Margarida Serra, Rita Malpique, Sofia Leite, Rui Tostões, Ana Amaral. Supervision of MIT Portugal PhD program Lab Rotations: Eunice Costa (2009); Inês Costa (2011); Daniel Simão (2012). Supervision of Research Grantees and Research Technicians (MScs and PhDs) : Ana Mendes (2009), Ivette Pacheco (2010), Inês Costa (2010/11), Antónia Pinto (2010), Daniel Simão (2010/11), Cristina Pereira (2009/10, 2011/12), Marta Silva and Marta Estrada (since 2011). Co Supervision of Post Doc Fellows: Telma de Palma (2012), Patrícia Alves (since 2011). 3/6

4 Co Supervision of PhD students: Sofia Rebelo (2010/14), Daniel Simão (2011/15), Inês Figueira (2012/2016). Supervision of Master Students: Ana Terrasso (2011/12), Catarina Pinto (2011/12), Inês Silva (2012/13 cosupervision). Member of PhD Committees: Rita Malpique (2010), Margarida Serra (2011), Rui Tostões (2011). Participation in Juries Master Thesis Juries: Ana Rita Almeida de Oliveira, FC UL, PhD Thesis Juries: Rita Malpique, ITQB UNL, Teaching Activity: Since 2011/12: Lecturer at the ITQB PhD program Since 2008/09: Invited Teaching Assistant in Biochemical Engineering, at the Master Program "Mestrado Integrado Engenharia Química e Bioquímica", Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa. Since 2008/09: Invited Lecturer on several Master programs Faculdade de Ciências, Universidade de Lisboa; Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa; Instituto Superior de Agronomia, Lisboa. 2009/10: Invited Lecturer on PhD program at Faculdade de Ciências Médicas da Universidade Nova de Lisboa. PUBLICATIONS Thesis Biochemistry Degree Thesis: Importance of the transmembrane domain of human fucosyltransferase III for its Golgi localization and in vivo activity, 2001, Faculdade de Ciências da Universidade de Lisboa. Ph.D. Thesis: Fucosyltransferase IX: characterization and biological role, 2007, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa. Papers under invitation 1. Serra M, Brito C and Alves PM (2010) Bioengineering strategies for stem cell expansion and differentiation Canal Bioquímica 7, pdf Papers in Peer Reviewed Journals 1. Sousa V, Brito C, Costa T, Lanoix J, Nilsson T, Costa J (2003) Importance of Cys, Gln and Tyr from the transmembrane domain of human α3/4 fucosyltransferase III for its localization and sorting in the Golgi of baby hamster kidney cells J. Biol. Chem. 278, IF= Sousa V, Brito C, Costa J (2004) Deletion of the cytoplasmic domain of human alpha 3/4 fucosyltransferase III causes the shift of the enzyme to early Golgi compartments Biochim. Biophys. Acta Gen. Subj. 1675, IF= Escrevente C, Machado E, Brito C, Reis CA, Stoeck A, Runz S, Marmé A, Altevogt P, Costa J (2006) Different expression levels of α3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines Int. J. Oncol. 29, IF= publications.com/ijo/29/3/ Morais VA, Brito C, Pijak DS, Crystal AS, Fortna RR, Li T, Wong PC, Doms RW, Costa J (2006) N glycosylation of human nicastrin is required for interaction with the lectins from the secretory pathway calnexin and 4/6

5 ERGIC 53 Biochim. Biophys. Acta Mol. Basis Dis. 1762, IF= Brito C, Escrevente C, Reis CA, Lee VM Y, Trojanowski, JQ, Costa J (2007a) "Increased levels of fucosyltransferase IX and carbohydrate Lewis x adhesion determinant in human NT2N neurons" J. Neurosci. Res. 85, IF= Serra M, Leite SB, Brito C, Costa J, Carrondo MJT, Alves PM (2007) "A novel culture strategy for human stem cell proliferation and neuronal differentiation" J. Neurosci. Res. 85, IF= Brito C, Gouveia R, Costa J (2007b) "Stable expression of an active soluble recombinant form of human fucosyltransferase IX in Spodoptera frugiperda Sf9 cells Biotech. Letters 11, IF= x 8. Brito C, Kandzia S, Graça T, Conradt H, Costa J (2008) Human fucosyltransferase IX: Specificity towards N linked glycoproteins and relevance of the cytoplasmic domain in intra Golgi localization Biochimie 90, Chosen by editors as cover image of Biochimie, Vol 90, Issue 9. IF= Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, Carrondo MJT, Alves PM (2009a) "Stirred bioreactors for the expansion of adult pancreatic stem cells " Ann. Anat. 191, Special Issue Stem Cell Research, CELLPROM European Project, IF= Brito C, Danglot L, Galli T, Costa J (2009) "Subcellular localization of the carbohydrate Lewis x adhesion structure in hippocampus cell cultures", Brain Res. 1287, IF= Serra M, Brito C, Costa E, Sousa MFQ, Alves PM (2009b) Integrating human stem cell expansion and neuronal differentiation in bioreactors, BMC Biotechnol. 9:82. Ranked highly accessed. IF= Malpique R, Osório L, Ferreira D, Ehrhart F, Katsen Globa A, Brito C, Zimmermann H, Alves PM (2010) Alginate encapsulation for cryopreservation of brain cell aggregates, Tissue Eng. Part C Methods 16, IF= Serra M, Brito C, Sousa MFQ, Jensen J, Strehl R, Hyllner J, Tostões R, Clemente J, Carrondo MJT, Alves PM (2010) Improving expansion of pluripotent hescs in perfused bioreactors through oxygen control, J. Biotechnol. 148, IF= Serra M, Correia C, Malpique R, Brito C, Jensen J, Bjorquist P, Carrondo MJT, Alves PM (2011) Microencapsulation technology: a powerful tool to integrate expansion and cryopreservation of pluripotent human embryonic stem cells, PloS One 6, p. e IF= Tostões R, Leite S, Serra M, Jensen J, Björquist P, Carrondo MJT, Brito C, Alves PM (2012) Human liver cell spheroids in extended perfusion bioreactor culture for repeated dose drug testing, Hepatology 55, IF= Marcelino I, de Almeida AM, Brito C, Meyer DF, Barreto M, Sheikboudou C, Franco CF, Martinez D, Lefrançois T, Vachiéry N, Carrondo MJT, Coelho AV, Alves PM (2012) Proteomic analyses of Ehrlichia ruminantium highlight diferential expression of MAP1 family proteins, Veterinary Microbiology 156, IF= Brito C, Simão D, Costa I, Malpique R, Pereira CI, Fernandes P, Serra M, Schwarz S, Schwarz J, Kremer EJ, Alves PM (2012), 3D cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification, Methods 56, IF= /6

6 18. Serra M, Brito C, Correia C, Alves PM (2012) Process engineering of human pluripotent stem cells for clinical application, Trends in Biotechnology 30, IF= Malpique R, Tostões RM, Beier A, Serra M, Brito C, Schulz J, Björquist P, Zimmermann H, Alves PM (2012) Surface based cryopreservation strategies for human embryonic stem cells: a comparative study, Biotechnology Progress, 8(4): Conference Proceedings 1. Simão D, Costa I, Serra M, Schwarz J, Brito C*, Alves PM (2011) "Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture, BMC Proceedings, 5 (Suppl 8):P S8 P53 * Corresponding author OTHER CV HIGHLIGHTS Scientific outputs, as in September 2012: 19 publications in international peer reviewed journals: sum of times cited: 132; average citation per article: 6.95; h index: 7. Invited lectures in conference cycles (5) and international courses (1); Invited for 4 presentations in international conferences, of a total of 25 oral communications and 69 poster communications. Expert reviewer for the international committee: Equipment of Excellence 2011 (Labex 2011), French National Research Agency (ANR). Member of the new ITQB PhD Program Workgroup Coordinator of the Pharmaceutical Biotechnology Profile. Member of the Workgroup for definition of "Laboratório Associado de Oeiras" Strategic Areas (2012). Reviewer for Tissue Engineering, PloS One, Journal of Biotechnology, Brain Research, Journal of Neuroscience, Journal of Tissue Engineering and Regenerative Medicine, BMC Biotechnology, etc. Science and Society: Participation in the Ciência Aberta initiatives (organized by Ministério da Ciência and Tecnologia, Fundação para a Ciência e Tecnologia); participation in ITQB Open Day target: people from Oeiras town Municipality; participation in several TV programs; participation in the initiative A researcher in your school within the Science and Technology Week target: high school students; lectures and debates about ethical issues associated to the application of Stem Cells and in lectures about the Three Rs Principle (Replacement, Reduction and Refinement of Animal Experimentation) and the tools available for its implementation target: high school students. 6/6