BIO 260L Microbiology Gram+ Dichotomous Key SP 14 - Fairborn
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1 BIO 260L Microbiology Gram Dichotomous Key SP 14 - Fairborn Gram Stain Blue Morphology Cocci Bacilli Catalase Test Bacillus subtilis Bubbles - No Bubbles Alpha hemolysis Beta hemolysis (double zone) Gamma hemolysis Gamma hemolysis Beta hemolysis (single zone) Staphylococcus aureus Staphylococcus epidermidis Enterococcus faecalis Streptococcus pyogenes MSA - MSA - Pink TSA Small white colonies TSA - Tiny pinpoint white colonies
2 BIO 260L Microbiology Gram- Dichotomous Key SP 14 Gram Stain - Red Green pigmentation on TSA (48h) - Not green Green Lactose Test aeruginosa - Pink Oxidase Blue Urease Test SIM Test: Indole Pink (delayed) - Red ring - No red ring Klebsiella pneumoniae Escherichia coli Proteus mirabilis SIM: -Indole No red ring SIM: Indole, red ring SIM: Moderate Motility SIM: Highly Motile
3 BIO 260L Microbiology Gram Dichotomous Key WI 13 Gram Stain Blue Morphology Cocci Bacilli Catalase Test Bacillus subtilis Bubbles - No Bubbles Alpha hemeolysis Beta hemeolysis (double zone) Gamma hemeolysis Alpha hemolysis Beta hemolysis (single zone) Staphylococcus aureus Staphylococcus epidermidis Streptococcus pneumoniae Streptococcus pyogenes MSA - MSA - Pink TSA Small white colonies TSA - Tiny pinpoint colonies
4 Gram (Blue- purple) Coccus Staphylococcus aureus Staphylococcus epidermidis Enterococcu faecalis Streptococcus pyogens Micrococcus leuteus Morphology Rods Bacillus subtilis : Alpha hemolysis Catalase (H2O2) Bubbles - Bubbles Micrococcus Enterococcus S. pyognes Off white MSA TSA- Pigment Pink /Orange Micrococcus Gama hemolytic Beta hemolytic S. pyogenes TSA Tiny Pinpoint Colonies Alpha hemolytic Enterococcus TSA Small Off White Colonies Double zone of Beta hemolysis Alpha/Beta hemolysis
5 Gram (Pink Red) Lactose - Pink citrate (blue) Black ppt Proteus mirabilis aeurginosa Serratia marcescens Proteus vulgasris Proteus mirabilis - No SIM - Sulfur Black ppt Serrita SIM -Indole red ring - No red ring Proteus mirabilis Oxidase - Serratia SIM Slightly motile SIM Highly motile Green on TSA 48hrs Pink on TSA 25 C
6 Blue Oxidase - White Gram (Pink Red) Green on TSA 48hrs Klebsiella Serrtia Proteus mirablils E. Coli Citrate Green TSA pigmentation - White E.coli Klebisella Serrita Lactose Klebisella Citrate Blue - Pink Indole red ring Serratia TSA 25C Proteus mirabilis SIM - Motility Motile Highly Motile Proteus mirabilis Indole No red ring
7 GRAM STAIN Gram Negative Gram Positive Rod Cocci Rod Cocci Oxidase TEST Catalase TEST Lactose ferm on MAC Hemolysis TEST Mannitol plate / acid Alpha Beta H₂S prod in SIM Optochin InhibiDon P. vulgaris P. aeruginosa S. pneumoniae S. pyogenes
8
9 S. epidemidis E. faecalis S. pyogens blue Gram Stain -red Salmonella P. mirabilis Pink Staphyloccous epidermidis Coagulase : Gammahemolytic MSA Catalase bubbles - bubbles Staphyloccous aureus Coagulase : Double zone of Betahemolysis E. faecalis S. pyogens Bacitracin Resistant Enteroccous faecalis : Gammahemolysis Inhibited Streptococcous pyogens : Beta-hemolysis A Gas A Escherichia coli TSA: white pigmentation Red TSI Mackonkey s K K Gas - aeruginosa TSA: green pigmentation after 48hrs Colorless Salmonella P. mirabilis K A Bppt Salmonella enteritidis TSA: white pigmentation TSI K Bppt Proteus mirabilis TSA: yellow pigmentation BIO 260L Microbiology Lab 8 and 9 Dichotomous Key for Lab Exercises 45, 46, 48 Bacterial Diseases of the Skin, Respiratory, and GI Systems
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