Biology 9/10/13. The Cell Membrane. Overview. Phospholipids. Phospholipid bilayer
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1 Biology 9/10/13 Warm-Up 1. What is the function of the cell? 2. What is the cell composed of? Learning Goal Understand the relationship between the structure of a cell and the transport of molecules The Cell Membrane Overview Cell separates living cell from nonliving surroundings thin barrier = 8nm thick Controls traffic in & out of the cell selectively permeable allows some substances to cross more easily than others hydrophobic vs hydrophilic Made of phospholipids, proteins & other macromolecules Phospholipids Fatty acid tails hydrophobic Phosphate group head hydrophilic Arranged as a bilayer Phosphate Fatty acid Aaaah, one of those structure function examples Phospholipid bilayer polar hydrophilic heads Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer Extracellular fluid Carbohydrate nonpolar hydrophobic tails polar hydrophilic heads Phospholipids Cytoplasm Trans proteins cytoskeleton 1
2 Membrane Proteins Proteins determine s specific functions Membrane proteins: peripheral proteins loosely bound to surface of cell surface identity marker (antigens) integral proteins penetrate lipid bilayer, usually across whole trans protein transport proteins channels, permeases (pumps) Many Functions of Membrane Proteins Outside Plasma Inside Transporter Enzyme activity Cell surface receptor Cell surface identity marker Cell adhesion Attachment to the cytoskeleton Membrane Carbohydrates Play a key role in cell-cell recognition ability of a cell to distinguish one cell from another antigens important in organ & tissue development basis for rejection of foreign cells by immune system Cell Membrane Mini-Poster Project Diffusion Move from HIGH to LOW concentration passive transport no energy needed movement of water Movement across the Cell Membrane osmosis 2
3 Diffusion across cell Cell is the boundary between inside & outside separates cell from its environment Can it be an impenetrable boundary? IN food carbohydrates sugars, proteins amino acids lipids salts, O 2, IN OUT OUT waste ammonia salts CO 2 products cell needs materials in & products or waste out NO! Diffusion through phospholipid bilayer What molecules can get through directly? fats & other lipids inside cell NH 3 lipid sugar aa outside cell salt What molecules can NOT get through directly? polar molecules ions salts, ammonia large molecules starches, proteins Channels Through the Cell Membrane Membrane becomes semi-permeable with protein channels inside cell specific channels allow specific material across cell aa sugar Facilitated Diffusion Diffusion through protein channels channels move specific molecules across cell no energy needed open channel = fast transport high facilitated = with help NH 3 salt outside cell low The Bouncer Active Transport Cells may need to move molecules against concentration gradient protein pump costs energy = low high conformational change The Doorman Transport Summary Passive Transport Simple of nonpolar, hydrophobic molecules lipids high low concentration gradient Facilitated transport of polar, hydrophilic molecules through a protein channel high low concentration gradient Active transport against concentration gradient low high uses a protein pump requires 3
4 Transport summary simple facilitated How about large molecules? Moving large molecules into & out of cell through vesicles & vacuoles endocytosis phagocytosis = cellular eating pinocytosis = cellular drinking exocytosis active transport exocytosis The Special Case of Water Movement of water across the cell Osmosis is of water Diffusion of water from high concentration of water to low concentration of water across a semi-permeable Concentration of water Direction of osmosis is determined by comparing total solute concentrations Hypertonic - more solute, less water Managing water balance Cell survival depends on balancing water uptake & loss Hypotonic - less solute, more water Isotonic - equal solute, equal water water hypotonic hypertonic net movement of water freshwater balanced saltwater 4
5 Passive Transport Lab 5
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