IFAC CNR. Francesco Baldini Ambra Giannetti Andrea Mencaglia Cosimo Trono Giacomo Ghini Folco Senesi
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1 Chemical and biochemical optical sensor group IFAC CNR Francesco Baldini Ambra Giannetti Andrea Mencaglia Cosimo Trono Giacomo Ghini Folco Senesi
2 Optical chemical and biochemical sensors SPECIFIC SENSORS Continuous monitoring of gastric carbon dioxide Continuous monitoring of interstitial ph DNA-based sensor for NF-kB Immunosensors for CRP and procalcitonin OPTICAL PLATFORMS FOR BIOCHEMICAL SENSING interrogation of microcantilevers interrogation of optical biochips
3 Continuous monitoring of gastric carbon dioxide 0,6 0,5 CO 2 [hpa] 0 optoelectronic unit absorbance 0,4 0,3 0, optical fibre link 0,1 147 optical fibre probe optical fibre sensitive membrane 0, plastic cap wavelength [nm] European project: COMOCADOF_Continuous Monitoring of Gastric Carbon Dioxide with Optical Fibres
4 Microdialysis-based continuous monitoring of interstitial ph Adipose tissue appears to be a reliable alternative site in stress conditions IP European project: CLINICIP_Closed Loop Insulin Infusion for Critically Ill Patients
5 DNA based sensor for active NF-kB A A G G G A C T T T C C A G G NH 2 3 A C C C T G A A A G G T C C 5 G Cy5 + DNA sequence labelled with Cy5 glass capillary labelled DNA sequence fluorescence p50 p50 + labelled NF-kB+ BHQ-3 NF-kB labelled with the BHQ-3 quencher biological sample fluorescence increase
6 Optical system for microcantilever interrogation Line shaped beam impinging the microcantilevers National Project Development and realisation of biosensors for the determination of messenger-rna in biological fluids
7 Optical PMMA chip for multianalyte detection Optical platform based on the anisotropy of the fluorescence of a fluorophore immobilised at a medium interface Implementation of bioassays for the detection of sepsis in POCT (C-reactive protein, procalcitonin) IP European project: CAREMAN_HealthCARE by biosensor Measurements And Networking
8 C-reactive protein Procalcitonin
9 Activity in progress Chemical and biochemical optical sensor group Stand-alone optical platform for POCT applications and immunosensors for sepsis detection (interleukines, neopterin, TNF- ) (European Project CAREMAN, in collaboration with Datamed srl, Milano) Nanosensors for intracellular applications (Regional project ICT-ONE, in collaboration with ICCOM-CNR and Faculty of Pharmacy-Pisa Univ.) Photonic Crystal Biosensors (National project, PRIN, in collaboration with Faculty of Engineering, Pisa Univ. and with Dept.of Electronics, Pavia Univ.) Chemical Sensors for diamines (putrescine and cadaverine) (in collaboration with Department of Chemical Sciences, Padova Univ.) Gastric ph sensor (in collaboration with Cecchi srl, Firenze)
10 Relevant publications in optical biosensors F. Baldini, L. Bolzoni, A. Giannetti, M. Kess, P. M. Krämer, E. Kremmer, G. Porro, F. Senesi, C. Trono, A new procalcitonin optical immunosensor for POCT applications, Anal. Bioanal. Chem., 393, pp , 2009 F. Baldini, A. Carloni, A. Giannetti, G. Porro, C. Trono, An optical PMMA biochip based on fluorescence anisotropy: application to C reactive protein assay, Sens. Actuat. B, 139, pp , 2009 F. Baldini, A. Carloni, A. Giannetti, A. Mencaglia, G. Porro, C. Trono, Optical PMMA chip for multianalyte detection, IEEE Sensors, 8 (7), pp , 2008 F. Baldini, A. Carloni, A. Giannetti, G. Porro, C. Trono, A new optical platform for biosensing based on fluorescence anisotropy, Anal. Bioanal. Chem., 391, pp , 2008 F.Baldini, A.Giannetti, A.Mencaglia, "Optical Sensor for interstitial ph measurements, J. Biom. Opt., 12 (2), (2007) A.Giannetti, L.Citti, C.Domenici, L.Tedeschi, F.Baldini, M.B. Wabuyele, T.Vo-Dinh, "FRET based protein- DNA binding assay for detection of active NF-kB, Sens. Actuat. B, 113 (2), pp , 2006 F.Baldini, "In vivo monitoring of the gastrooesophageal system using optical fibre sensors, Anal. Bioanal. Chem., 375, pp , 2003 L.Tedeschi, C.Domenici, A.Ahluwalia, F.Baldini, A. Mencaglia, "Antibody immobilisation on fibre optic TIRF sensor, Biosens. & Bioelectr., 19 (2), pp , 2003 F. Baldini, A. Falai, A.R. De Gaudio, D. Landi, A. Lueger, A. Mencaglia, D. Scherr, W. Trettnak, "Continuous monitoring of gastric carbon dioxide with optical fibres, Sens. Actuat. B, 90, pp , 2003 C.Preininger, A. Mencaglia, F. Baldini, Polymer-coated optical fibres for application in a direct evanescent wave immunoassay, Anal. Chim. Acta, 403, pp.67-76, 2000.
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