SPECIMEN REQUIREMENTS. Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment

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Bathsheba Nicholson
7 years ago
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1 Calprotectin, Fecal Calprotectin, Fecal Order Name: CALPRO FEC Test Number: LOINC Code: Quantitative Enzyme Immunoassay Preferred 5g (1g) Stool, Raw Sterile Screwtop Container 5g (1g) Stool unpreserved collected in a Sterile leak-proof container. Do not add fixative or preservative. Specimen Stability: Room temperature: 11 days, Refrigerated: 11 days, : 1 year Mon-Sat 2-4 Days Diagnose inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis. Differentiate IBD from irritable bowel syndrome (IBS). CPT Code(s) 83993

2 Dehydroepiandrosterone Unconjugated (DHEA) Order Name: DHEA UNC Test Number: LOINC Code: Dehydroepiandrosterone Unconjugated (DHEA) Quantitative HPLC/Tandem Mass Spectrometry Preferred 1 ml (0.3 ml) Serum Clot Activator (Red Top, No-Gel) Refrigerated Alternate 1 1 ml (0.3 ml) Plasma EDTA (Lavender Top) Refrigerated Alternate 2 1 ml (0.3 ml) Plasma Lithium Heparin PST (Light Green Top) Refrigerated Collect between 6-10 a.m. Separate serum or plasma from cells ASAP or within 2 hours of collection. Stability: After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; : 6 months Sun-Sat 2-5 days CPT Code(s) 82626

3 Ethosuximide (Zarontin) Ethosuximide (Zarontin) Order Name: ETHOSUXIM Test Number: LOINC Code: Quantitative Enzyme Immunoassay Preferred 1 ml (0.5) Serum Clot Activator (Red Top, No-Gel) Refrigerated Alternate 1 1 ml (0.5) Plasma EDTA (Lavender Top) Refrigerated Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration. Separate serum or plasma from cells ASAP or within 2 hours of collection. Unacceptable Conditions: Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution). Stability: After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; : 2 months. Tue, Wed, Sat 2-6 Days Ethosuximide is an anticonvulsant used to treat patients with petit mal, myoclonic, and akinetic seizures. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity. CPT Code(s) 80168

4 HE4, Ovarian Cancer Monitoring HE4, Ovarian Cancer Monitoring Order Name: HE4 Test Number: LOINC Code: Quantitative Enzyme Immunoassay Preferred 0.5mL (0.3mL) Serum Clot Activator (Red Top, No-Gel) Alternate 1 0.5mL (0.3mL) Serum Clot Activator SST (Red/Gray or Tiger Top) Allow specimen to clot completely at room temperature. Unacceptable Conditions: Hemolyzed or lipemic specimens. Stability: Ambient: 4 hours; Refrigerated: 72 hours; : 60 days Thr, Sun 2-9 Days HE4 is a biomarker for ovarian cancer. CPT Code(s) 86305

5 IGF Binding Protein-3 (IGFBP-3) IGF Binding Protein-3 (IGFBP-3) Order Name: IGF-3 Test Number: LOINC Code: Quantitative Chemiluminescent Immunoassay Preferred 0.5 ml (0.3 ml) Serum Clot Activator SST (Red/Gray or Tiger Top) Alternate ml (0.3 ml) Plasma Sodium Heparin (Green Top, No-Gel) Collect: Serum separator tube. Also acceptable: Green (sodium heparin). Unacceptable Conditions: Tissue or urine. Grossly hemolyzed or lipemic specimens. Stability: After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; : 1 year Sun-Sat 2-3 Days CPT Code(s) 82397

6 Lipoprotein (a) Order Name: LIPOPROT A Test Number: LOINC Code: Lipoprotein (a) Quantitative Immunoturbidimetric Preferred 1 ml (0.5) Serum Clot Activator SST (Red/Gray or Tiger Top) Refrigerated Alternate 1 1 ml (0.5) Plasma EDTA (Lavender Top) Refrigerated Alternate 2 1 ml (0.5) Plasma Lithium Heparin PST (Light Green Top) Refrigerated Serum separator tube or plasma separator tube. Also acceptable: Green (sodium or lithium heparin), lavender (EDTA). Allow serum to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Unacceptable Conditions: Body Fluids. Sun-Sat 2-3 Days Elevated concentrations of Lp(a) are associated with increased risk of coronary artery disease. CPT Code(s) 83695

7 Magnesium, RBC Order Name: MAG RBC Test Number: LOINC Code: Magnesium, RBC Quantitative Inductively Coupled Plasma-Mass Spectrometry Preferred 2 ml (0.6 ml) Whole Blood EDTA (Royal Blue Top/Trace Element Free) Room Temperature Collect: Royal blue (EDTA). Centrifuge whole blood and separate RBCs from plasma within 2 hours of collection. Transfer 2 ml RBCs to a Trace Element-Free Transport Tube. Unacceptable Conditions:. Clotted or grossly hemolyzed specimens. Stability: After separation from plasma: Ambient: 1 week; Refrigerated: 2 weeks; : Unacceptable Mon, Wed-Sat 2-4 Days CPT Code(s) 83735

8 Pancreatic Elastase, Fecal Pancreatic Elastase, Fecal Order Name: STOOL ELAS Test Number: LOINC Code: Enzyme-Linked Immunosorbent Assay Preferred 5g (1g) Stool, Random Sterile Screwtop Container Interruption of enzyme substitution therapy recommended in order to avoid the possibility of cross-reaction with porcine enzymes. Transfer 5 g stool to an unpreserved stool transport vial. Unacceptable Conditions: Stool in media or preservative. Swabs. Stability: Ambient: 5 days; Refrigerated: 1 week; : 1 year. Sun, Tue-Fri 2-5 days The Elastase-1 is a quantitative enzyme linked immunosorbent assay for measuring concentrations of elastase-1 in feces as an aid in diagnosis of the exocrine pancreatic function. CPT Code(s) 83520

9 Primidone (Mysoline) Level Order Name: PRIMID REF Test Number: LOINC Code: Primidone (Mysoline) Level Immunoassay Preferred 1mL (0.8 ml) Serum Clot Activator (Red Top, No-Gel) Refrigerated Alternate 1 1mL (0.8 ml) Plasma Sodium Heparin (Green Top / No-Gel) Refrigerated Collect just prior to next dose. Collect: Plain red. Also acceptable: Green (sodium heparin). Allow serum to clot completely at room temperature. Separate serum from cells within 2 hours of collection. Unacceptable Conditions: Separator tubes. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 1 week; : 1 month. Sun-Sat 2-3 Days CPT Code(s) 80188

10 Procalcitonin Order Name: PROCALCITN Test Number: LOINC Code: Procalcitonin Immunofluorescent Assay Preferred 2 ml (0.5 ml) Serum Clot Activator SST (Red/Gray or Tiger Top) Alternate 1 2 ml (0.5 ml) Plasma EDTA (Lavender Top) Alternate 2 2 ml (0.5 ml) Plasma Lithium Heparin PST (Light Green Top) The same specimen type (serum, plasma) should be used throughout the patient's clinical course. Collect: Plasma separator tube or serum separator tube. Allow serum to sit for minutes for proper clot formation and to ensure the absence of fibrin in the serum which can interfere with this assay. Separate serum or plasma from cells within 2 hours of collection. Unacceptable Conditions: Specimens collected in citrate anticoagulant. Stability: After separation from cells: Ambient: 24 hours; Refrigerated: 5 days; : 1 month. Sun-Sat 2-3 Days Used in the diagnosis of bacteremia and septicemia in adults and children (including neonates), renal involvement in urinary tract infection in children, bacterial infection in neutropenic patients, and in the diagnosis, risk stratification, and monitoring of septic shock, systemic secondary infection post-surgery, as well as in severe trauma, burns, and multiorgan failure. Differential diagnosis of bacterial versus viral meningitis. Differential diagnosis of community-acquired bacterial versus viral pneumonia. Monitoring of therapeutic response to antibacterial therapy. CPT Code(s) 84145

11 Thyroxine Binding Globulin (TBG) Thyroxine Binding Globulin (TBG) Order Name: TBG Test Number: LOINC Code: Quantitative Chemiluminescent Immunoassay Preferred 0.5 ml (0.4 ml) Serum Clot Activator SST (Red/Gray or Tiger Top) Unacceptable Conditions: Plasma, tissue or urine. Grossly hemolyzed or lipemic specimens. Stability: Ambient: 24 hours; Refrigerated: 1 week; : 1 month. Mon, Wed, Fri 2-5 Days CPT Code(s) 84442

12 Trypsin (Trypsinogen) Trypsin (Trypsinogen) Order Name: TRYPSIN Test Number: LOINC Code: Radioimmunoassay Preferred 1 ml (0.3) Serum Clot Activator SST (Red/Gray or Tiger Top) Allow serum tube to sit for minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection Unacceptable Conditions: Heparinized specimens. Hemolyzed or lipemic specimens. Stability: After separation from cells: Ambient: 2 hours; Refrigerated: 24 hours; : 3 months. Tues, Fri 2-6 Days Trypsin (or trypsinogen) is considered a specific indicator of pancreatic damage. Increased values over the determined normal range may indicate inflammatory pancreatic condition. CPT Code(s) 83519

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605 LACTIC ACID Fasting, arterial specimen preferred. Please note whether arterial or venous. 0.5 ml heparinized plasma. Green top or PST must be drawn, placed on ice, and spun within 15 minutes. Immediately