Chapter : DNA: The Molecule of Heredity
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1 1 Chapter : DNA: The Molecule of Heredity DNA Deoxyribonucleic acid Is a type of nucleic acid What chromosomes (and genes) are made of Made up of repeating nucleotide subunits 1 nucleotide looks like: Phosphate Group Deoxyribose Sugar Nitrogenous Base 2 strands so bases can pair up o A binds T only o C binds G only Strand 1 Strand 2 P P 4 types: Adenine (A) Guanine (G) Cytosine (C) Thymine (T) Remember S A T S DNA is like an Oreo P P S C G S Phophates + sugars = cookies Bases = cream filling Phosphates + sugars on the outside Bases on the inside (Bases fit like puzzle pieces)
2 2 Shape is a double helix o Double helix: 2 spirals wound around each other o Rosalind Franklin took an X-ray photo of DNA o James Watson and Francis Crick interpreted the photo and discovered the double helix structure (They won the Nobel Prize) Codon: Group of 3 bases Genes: stretch of DNA that codes for a trait o The code is the order of the bases (letters) o Genes are hundreds or thousands of bases long Eye color gene Dimples gene Hair color gene
3 3 Chargaff s Rule In DNA, the amount of A = the amount of T the amount of C = the amount of G DNA is complementary Complementary: bases on one strand match up with the bases on the other strand (A-T and G-C) Example: Strand 1- ATG GGC CTA Strand 2- TAC CCG GAT Replication Process by which DNA copies itself Happens when chromosomes copy themselves before mitosis and meiosis Semiconservative replication: Each new piece of DNA is made up of 1 old strand and 1 new strand Original DNA DNA unzips Each original strand grows a new strand
4 4 DNA never ever leaves the nucleus DNA is the master copy of the directions a cell needs to live so it needs to be protected DNA in the nucleus is safe But DNA in the cytoplasm can be destroyed RNA is a copy of DNA that goes out into the cytoplasm to tell the cell what to do in order to stay alive RNA: ribonucleic acid You can always make more RNA so it s ok if it gets destroyed (You can t make more DNA!!!) How many strands? DNA RNA 2 1 Nucleotide subunit Phosphate Group Deoxyribose Sugar Nitrogen Base Phosphate Group Ribose Sugar Nitrogen Base Bases Deoxyribose sugar Thymine (T) Adenine (A) Guanine (G) Cytosine (C) T A G C Uracil (U) Adenine (A) Guanine (G) Cytosine (C) Ribose sugar U A G C
5 5 Transcription Definition: RNA is made from 1 gene in DNA The type of RNA made is called mrna (messenger RNA) because it sends a message from DNA to the cytoplasm DNA safe in the nucleus Uses mrna To send a message to the cytoplasm Transcription o Unzip one gene in DNA o Match up bases to one side of a gene in DNA o mrna detaches from the DNA o mrna moves out of the nucleus and into the cytoplasm DNA: GAG AAC TAG TAC RNA: CUC UUG AUC AUG For figuring out RNA: A binds U C binds G
6 6 DNA mrna Nucleus mrna Cytoplasm of cell Transcription happens in the nucleus. An RNA copy of a gene is made. Then the mrna that has been made moves out of the nucleus into the cytoplasm Once in the cytoplasm, the mrna is used to make a protein How does mrna tell the cell what to do? mrna is a message that codes for a protein Proteins are made in the cytoplasm and then work to keep the cell alive Translation (protein synthesis): Process of making a protein Proteins are made up of amino acids (small building blocks) There are 20 different types of amino acids Protein Amino Acids
7 7 Nucleus Process of Translation 1. mrna moves out of nucleus and into cytoplasm Cytoplasm 2. mrna attaches to a ribosome 3. Transfer RNA (trna) decodes the mrna and brings amino acids to build up the protein Ribosome trna Amino acid Anticodon (3 bases on trna): Matches up to codons on mrna 4. Protein (chain of amino acids) detaches from ribosome and goes off to work in the cell
8 8 Genetic Code Code that matches codons in mrna to amino acids on trnas mrna codons (3 bases) Amino acids Stop codon codes for the end of the mrna (no amino acid added) 1. Read your mrna codon ACU 2. Find 1 st base on the left, 2 nd base on the top, 3 rd base on the right. Find where they all cross in the chart. 3. Read your amino acid. Threonine Different codons code for different amino acids!!!
9 9 Central dogma of molecular biology Transcription Translation DNA RNA Protein Directions to make proteins are safely stored in the nucleus Carries the directions to the cytoplasm Work to keep the cell alive Mutation a change in the DNA sequence It s a mistake that s made during replication or transcription can be harmful: diseases or deformities helpful: organism is better able to survive neutral: organism is unaffected if a mutation occurs in a sperm or egg cell, that mutation is passed onto offspring
10 10 if a mutation occurs in a body cell, that mutation affects only the organism and is not passed onto offspring Types of mutations 1. Point mutations: Bases are mismatched Harmful when: a mistake in DNA is carried into mrna and results in the wrong amino acid Correct DNA Correct mrna Correct amino acid GAG CTC CUC Leucine Point mutation in DNA Mutated mrna Wrong amino acid GCG CTC CGC Arginine A should pair with T, but instead C is mismatched to T Not harmful when: a mistake in DNA is carried into mrna but still results in the correct amino acid
11 11 2. Frameshift mutations: bases are inserted or deleted Are usually harmful because a mistake in DNA is carried into mrna and results in many wrong amino acids Correct DNA: ATA CCG TGA TAT GGC ACT Correct mrna: UAU GGC ACU Correct amino acids: Tyrosine Glycine Threonine Extra inserted base shifts how we read the codons (3 bases), which changes the amino acids Frameshift mutation ATG ACC GTG A in DNA: TAC TGG CAC T Mutated mrna: UAC UGG CAC U Wrong amino acids: Tyrosine Tryptophan Histadine
12 12 3. Chromosomal mutations chromosomes break or are lost during mitosis or meiosis broken chromosomes may rejoin incorrectly almost always lethal when it occurs in a zygote Causes of mutations mutagens: anything that causes a change in DNA examples: X rays, UV light, nuclear radiation, asbestos, cigarette smoke
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