Dimethylphenol Method Method HR (5 to 35 mg/l NO 3 N or 22 to 155 mg/l NO 3 ) TNTplus 836

Size: px
Start display at page:

Download "Dimethylphenol Method Method HR (5 to 35 mg/l NO 3 N or 22 to 155 mg/l NO 3 ) TNTplus 836"

Transcription

1 HR, DOC Dimethylphenol Method Method HR (5 to 35 mg/l NO 3 N or 22 to 155 mg/l NO 3 ) TNTplus 836 Scope and Application: For wastewater, drinking water, surface water and process water Test preparation How to use instrument-specific information The Instrument-specific information table displays requirements that may vary between instruments. To use this table, select an instrument then read across to find the corresponding information required to perform this test. Table 1 Instrument-specific information Instrument Light shield Adapter DR 5000 DR 2800 LZV646 Before starting the test: DR 2800: Install the light shield in Cell Compartment #2 before performing this test. Always read the Safety Advice and Expiration Date on package. Perform this test at the recommended temperature to avoid an incorrect result. The recommended sample and reagent temperature is C ( F). Analyze samples as soon as possible. Recommended sample ph is Recommended reagent storage is C (59 77 F). TNTplus methods are activated from the Main Menu when the sample vial is inserted into the sample cell holder. Collect the following items: Description Quantity Light Shield (DR 2800 only) 1 LR TNT 835 Reagent Set 1 Pipettor variable, ul 1 Pipettor Tip, for ul pipettor 2 See Consumables and replacement items for reorder information. Page 1 of 6

2 Dimethylphenol method 1. Pipet 0.2 ml (200 µl) of sample into the reagent vial. 2. Pipet 1.0 ml (1000 µl) of Solution A into the vial. 3. Cap and invert the reaction tube 2 3 times until no more streaks can be seen in the reaction tube solution. 4. Wait 15 minutes. 5. After the timer expires wipe the vial and insert the prepared vial into the cell holder. The instrument reads the barcode, then selects and performs the correct test. No Zero is required. Results are in mg/l NO 3 N Interferences The items listed in the Interfering substances table have been individually checked up to the given concentrations and do not cause interference. The cumulative effects and influence of other ions have not been determined. High loads of oxidizable organic substances (COD) cause the reagent to change color and to give high-bias results. The test can thus only be used for wastewater analyses if the COD is less than 500 mg/l. Measurement results can be verified using sample dilutions or standard additions. Nitrite concentrations of more than 2.0 mg/l interfere (high-bias results). Add 50 mg of sulfamic acid (amidosulfonic acid) to 5.0 ml of sample, dissolve and wait for 10 minutes. Analyze the prepared sample as described in the procedure above. Page 2 of 6

3 Table 2 Interfering substances Interfering substance K + Na + Cl COD Ca 2+ Ag + Pb 2+, Zn 2+, Ni 2+, Fe 3+, Cd 2+, Cu 2+ Fe 2+ Co 2+ Cr 6+ NO 2 Interference level 2000 mg/l 1500 mg/l 1000 mg/l 500 mg/l 250 mg/l 100 mg/l 50 mg/l 20 mg/l 10 mg/l 5 mg/l 2 mg/l Reagent blanks Sample blanks A reagent blank can be measured and the value subtracted from the results of each test performed using the same reagent lot number. Use deionized water in place of sample and run the Dimethylphenol method procedure as described. To subtract the value of the blank from a series of measurements: 1. Measure the blank per step Turn on the reagent blank function. The measured value of the blank should be displayed in the highlighted box. 3. Accept the blank value. The reagent blank value will be subtracted from all results until the function is turned off or a different method is selected. Alternately, the blank can be recorded and entered at any later time by pressing the highlighted reagent blank box and using the keypad to enter the value. Colored or turbid samples can cause high results. To compensate for color or turbidity, the procedure is repeated and the color forming reagent that is present in Solution A is not added. To determine the sample blank: 1. Perform the Dimethylphenol method with 1.0 ml of deionized water in place of the 1.0 ml of Solution A in step 2. Use the original cap to cap the sample vial. 2. Subtract the value obtained in step 5 from the value obtained on the original sample to give the corrected sample concentration. Alternatively, samples that contain only turbidity may be first filtered through a membrane filter and then analyzed. Samples without color or turbidity do not require sample blanks. Page 3 of 6

4 Sample collection, preservation and storage Accuracy check Collect samples in clean plastic or glass bottles. Analyze samples as soon as possible to prevent bacterial degradation of the nitrate. If immediate analysis is not possible, store at 4 C (39 F) or lower if the sample is to be analyzed within 24 to 48 hours. Warm to C before running the test. For longer storage periods (up to 14 days), adjust sample ph to 2 or less with Sulfuric Acid, ACS * (about 2 ml per liter). Sample refrigeration is still required. Before testing the stored sample, warm to C and neutralize with 5.0 N Sodium Hydroxide Standard Solution*. Do not use mercury compounds as preservatives. Correct the test result for volume additions. Standard solution method Note: Refer to the instrument user manual for specific software navigation instructions. Required for accuracy check: Nitrogen Standard, 10 mg/l NO 3 N or Wastewater Influent Mixed Parameters Inorganics Standard 1. Use 0.2 ml of nitrogen standard, 10 mg/l in place of the sample in step 1. or 2. Use 0.2 ml of Wastewater Influent Mixed Parameters Inorganics Standard in place of the sample in step 1. This standard contains 10 mg/l nitrate nitrogen combined with ammonia, phosphate, sulfate and organic material. 3. Follow the Dimethylphenol method test procedure. 4. To adjust the calibration curve using the reading obtained with the standard solution, navigate to Standard Adjust in the software. Instrument DR 5000 DR 2800 Navigate to: OPTIONS>MORE>STANDARD ADJUST OPTIONS>MORE>STANDARD ADJUST 5. Turn on the Standard Adjust feature and accept the displayed concentration. If an alternate concentration is used, enter the concentration and adjust the curve to that value. * See Optional reagents and apparatus. Page 4 of 6

5 Summary of method ions in solutions containing sulfuric and phosphoric acids react with 2,6-dimethylphenol to form 4-nitro-2,6-dimethylphenol. Test results are measured at the wavelengths in the Test wavelengths table. Table 3 Test wavelengths Instrument DR 5000 DR 2800 Wavelength 370 nm 345 nm Consumables and replacement items Required reagents Description Quantity/Test Unit Catalog number TNTplus, HR TNT /pkg TNT836 Required apparatus Description Quantity/Test Unit Catalog number Pipettor, variable volume, µl 1 each Pipettor Tips, for pipettor 2 400/pkg Recommended standards Description Unit Catalog number Nitrogen Standard Solution, 10-mg/L 500 ml Nitrogen Standard Solution, 1000 mg/l 500 ml Wastewater Influent Inorganics Standard for NH 3 N, NO 3 N, PO 4, COD, SO 4, TOC 500 ml Water, deionized 4 L Optional reagents and apparatus Description Unit Catalog number Balance, AccuLab VI Series, 120 g capacity each Bottle, sampling, low density poly, w/cap, 500 ml 12/pkg Filter Holder, glass, for vacuum filtration each Filter, membrane, 47 mm; 0.45-micron, hydrophilic, polyethersulfone each Flask, filtering, glass, 1000-mL each Sodium Hydroxide, 5.0 N 50 ml SCDB Sulfamic Acid 454 g Sulfuric Acid ACS, concentrated 500 ml Test Tube Rack for 13-mm vials each Tubing, rubber 12-ft Aspirator each Page 5 of 6

6 FOR TECHNICAL ASSISTANCE, PRICE INFORMATION AND ORDERING: In the U.S.A. Call toll-free Outside the U.S.A. Contact the HACH office or distributor serving you. On the Worldwide Web HACH COMPANY WORLD HEADQUARTERS Telephone: (970) FAX: (970) Hach Company, All rights reserved. Printed in the U.S.A. Updated February 2008, Edition 5

Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856

Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856 Nickel DOC316.53.01065 Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856 Scope and application: For water and wastewater. Test preparation Instrument-specific information Table 1 shows

More information

Cadmium Reduction Method Method 8039 0.3 to 30.0 mg/l NO 3 N (HR) Powder Pillows or AccuVac Ampuls

Cadmium Reduction Method Method 8039 0.3 to 30.0 mg/l NO 3 N (HR) Powder Pillows or AccuVac Ampuls Nitrate DOC316.53.01066 Cadmium Reduction Method Method 8039 0.3 to 30.0 mg/l NO 3 N (HR) Powder Pillows or AccuVac Ampuls Scope and application: For water, wastewater and seawater. Test preparation Instrument-specific

More information

Phosphorus, Reactive (Orthophosphate)

Phosphorus, Reactive (Orthophosphate) Phosphorus, Reactive (Orthophosphate) DOC316.53.01113 Amino Acid Method 1 Method 8178 0.23 to 30.00 mg/l PO 4 Reagent Solution Scope and application: For water, wastewater and seawater. 1 Adapted from

More information

Chlorine, Total. DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials. Test preparation. Instrument-specific information

Chlorine, Total. DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials. Test preparation. Instrument-specific information Chlorine, Total DOC316.53.01028 DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials Scope and application: For testing higher levels of total (free plus combined) chlorine in drinking water,

More information

USEPA 1 FerroVer Method 2 Method 8008 0.02 to 3.00 mg/l Fe Powder Pillows or AccuVac Ampuls

USEPA 1 FerroVer Method 2 Method 8008 0.02 to 3.00 mg/l Fe Powder Pillows or AccuVac Ampuls Iron, Total DOC316.53.01053 USEPA 1 FerroVer Method 2 Method 8008 0.02 to 3.00 mg/l Fe Powder Pillows or AccuVac Ampuls Scope and application: For water, wastewater and seawater; digestion is required

More information

FerroVer Method 1 Method 10249 0.1 to 3.0, 1.0 to 30.0 and 10.0 to 300.0 mg/l Fe Powder Pillows

FerroVer Method 1 Method 10249 0.1 to 3.0, 1.0 to 30.0 and 10.0 to 300.0 mg/l Fe Powder Pillows Iron, Total DOC316.53.01314 FerroVer Method 1 Method 10249 0.1 to 3.0, 1.0 to 30.0 and 10.0 to 300.0 mg/l Fe Powder Pillows Scope and application: For oil and gas field waters; digestion is required for

More information

Color, True and Apparent

Color, True and Apparent Color, True and Apparent DOC316.53.01037 Platinum-Cobalt Standard Method 1, 2, 3 Method 8025 15 to 500 color units Scope and application: For water, wastewater and seawater; equivalent to NCASI method

More information

LZV585 (B) The 1-cm path is aligned with the arrow on the adapter. 5940506

LZV585 (B) The 1-cm path is aligned with the arrow on the adapter. 5940506 Chlorine, Total DOC316.53.01029 USEPA DPD Method 1 Method 10070 0.1 to 10.0 mg/l Cl 2 (HR) Powder Pillows Scope and application: For testing higher levels of total chlorine (free and combined) in drinking

More information

Color, True and Apparent

Color, True and Apparent Color, True and Apparent DOC316.53.01037 Platinum-Cobalt Standard Method 1, 2, 3 Method 8025 15 to 500 color units 5 to 500 color units (low range technique) Scope and application: For water, wastewater

More information

Quaternary Ammonium Compounds DOC316.53.01128

Quaternary Ammonium Compounds DOC316.53.01128 Quaternary Ammonium Compounds DOC316.53.01128 Direct Binary Complex Method Method 8337 0.2 to 5.0 mg/l as CTAB (cetyl-trimethylammonium bromide) Powder Pillows Scope and application: For cooling tower

More information

Esterification Method 1 Method 8196 27 to 2800 mg/l (as acetic acid) Reagent Solution

Esterification Method 1 Method 8196 27 to 2800 mg/l (as acetic acid) Reagent Solution Volatile Acids DOC316.53.01144 Esterification Method 1 Method 8196 27 to 2800 mg/l (as acetic acid) Reagent Solution Scope and application: For digestor sludges. 1 Adapted from The Analyst, 87, 949 (1962).

More information

Method 8155 NITROGEN, AMMONIA (0 to 0.50 mg/l NH 3 -N) For water, wastewater, seawater

Method 8155 NITROGEN, AMMONIA (0 to 0.50 mg/l NH 3 -N) For water, wastewater, seawater Method 8155 NITROGEN, AMMONIA (0 to 0.50 mg/l NH 3 -N) For water, wastewater, seawater Salicylate Method * 1. Enter the stored program number for ammonia nitrogen (NH 3 -N). 2. Press: 64 ENTER The display

More information

DR/4000 PROCEDURE. CHLORINE, Free

DR/4000 PROCEDURE. CHLORINE, Free DR/4000 PROCEDURE CHLORINE, Free Method 10069 DPD method* UHR (0.1 10.0 mg/l as Cl 2 ) Scope and Application: For testing higher levels of free chlorine (hypochlorous acid and hypochlorite ion) in drinking

More information

Nitrogen, Ammonia. Known Addition ISE Method 1 Method 10002 Minimum of 0.8 mg/l NH 3 N. Ammonia ISE. Test preparation. Instrument-specific table

Nitrogen, Ammonia. Known Addition ISE Method 1 Method 10002 Minimum of 0.8 mg/l NH 3 N. Ammonia ISE. Test preparation. Instrument-specific table Nitrogen, Ammonia DOC316.53.01234 Known Addition ISE Method 1 Method 10002 Minimum of 0.8 mg/l NH 3 N Scope and application: For wastewater 2. Ammonia ISE 1 Adapted from the Standard Methods for the Examination

More information

Scope and application: For testing residual corrosion inhibitors (oxygen scavengers) in boiler feed water or condensate

Scope and application: For testing residual corrosion inhibitors (oxygen scavengers) in boiler feed water or condensate Oxygen Scavengers DOC316.53.01105 Iron Reduction Method Method 8140 3 to 450 µg/l DEHA; 5 to 600 µg/l carbohydrazide; 9 to 1000 µg/l hydroquinone; 13 to 1500 µg/l iso-ascorbic acid (ISA); 15 to 1000 µg/l

More information

Solids, Volatile Dissolved and Fixed Dissolved

Solids, Volatile Dissolved and Fixed Dissolved , 8277 Solids, Volatile Dissolved and Fixed Dissolved Gravimetric Method 1 Scope and Application: For wastewater. 1 Adapted from Standard Methods for the Examination of Water and Wastewater DOC316.53.001206

More information

Direct ISE Method Method 8359 10 to 1000 mg/l Na + Sodium ISE

Direct ISE Method Method 8359 10 to 1000 mg/l Na + Sodium ISE Sodium DOC316.53.01240 Direct ISE Method Method 8359 10 to 1000 mg/l Na + Sodium ISE Scope and application: For drinking water and process water. Test preparation Instrument-specific information This procedure

More information

Direct Measurement Method Method 8157 0 to 20.0 mg/l (or 0 to 200% saturation) O 2 Clark-type Amperometric Sensor

Direct Measurement Method Method 8157 0 to 20.0 mg/l (or 0 to 200% saturation) O 2 Clark-type Amperometric Sensor Oxygen, Dissolved DOC316.53.01241 Direct Measurement Method Method 8157 0 to 20.0 mg/l (or 0 to 200% saturation) O 2 Clark-type Amperometric Sensor Scope and application: For water, wastewater and process

More information

Lead Testing and On Site Calibration for Water Testing Detection Range: 2 100ppb

Lead Testing and On Site Calibration for Water Testing Detection Range: 2 100ppb Document: AND Lead 100 7 2013 Lead Testing and On Site Calibration for Water Testing Detection Range: 2 100ppb July, 2013 Edition 1 ANDalyze, Inc., 2012. All rights reserved. Printed in USA. Table of Contents

More information

USEPA Electrode Method Method 8156

USEPA Electrode Method Method 8156 ph DOC316.53.01245 USEPA Electrode Method Method 8156 Scope and application: For drinking water 1, wastewater 2 and process water. 1 Based on Standard Method 4500-H+B, ASTM Method D1293-95 and USEPA Method

More information

Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method)

Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method) Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method) Grace Analytical Lab 536 South Clark Street 10th Floor Chicago, IL 60605 April 15, 1994 Revision 2 Standard Operating Procedure

More information

Phosphorus, colorimetry, phosphomolybdate, automated-segmented flow

Phosphorus, colorimetry, phosphomolybdate, automated-segmented flow Phosphorus, colorimetry, phosphomolybdate, automated-segmented flow Parameter and code: Phosphorus, total-in-bottom-material, dry weight, I-6600-88 (mg/kg as P): 00668 1. Application This method is used

More information

NITRIC OXIDE and NITROGEN DIOXIDE 6014

NITRIC OXIDE and NITROGEN DIOXIDE 6014 NITRIC OXIDE and NITROGEN DIOXIDE 6014 NO MW: 30.01 CAS: 10102-43-9 RTECS: QX0525000 46.01 10102-44-0 QW9800000 METHOD: 6014, Issue 1 EVALUATION: FULL Issue 1: 15 August 1994 OSHA : 25 ppm NO; C 1 ppm

More information

NANYANG TECHNOLOGICAL UNIVERSITY. School of Civil and Environmental Engineering. CV2701: Laboratory 2A. Laboratory Manual. For

NANYANG TECHNOLOGICAL UNIVERSITY. School of Civil and Environmental Engineering. CV2701: Laboratory 2A. Laboratory Manual. For NANYANG TECHNOLOGICAL UNIVERSITY School of Civil and Environmental Engineering CV2701: Laboratory 2A Laboratory Manual For Experiment Lab 2A-6(ENV) Wastewater Quality Analysis Location : Environment Laboratory,

More information

Thermo Scientific Orion AQUAfast Powder and Tablet Reagent Chemistry User Guide

Thermo Scientific Orion AQUAfast Powder and Tablet Reagent Chemistry User Guide Thermo Scientific Orion AQUAfast Powder and Tablet Reagent Chemistry User Guide for use with AquaMate 7000 Vis and AquaMate 8000 UV-Vis Spectrophotometers 2012 Thermo Fisher Scientific Inc. All rights

More information

Chlorine Residual Testing Fact Sheet

Chlorine Residual Testing Fact Sheet Chlorine Residual Testing Fact Sheet The presence of free chlorine residual in drinking water indicates that: 1) a sufficient amount of chlorine was added to the water to inactivate most of the bacteria

More information

Measurement by Ion Selective Electrodes

Measurement by Ion Selective Electrodes Measurement by Ion Selective Electrodes Adrian Vazquez May 8, 2012 Why Use Ion Selective Electrodes? Responsive over a wide concentration range Not affected by color or turbidity of sample Rugged and durable

More information

Experiment 8 - Double Displacement Reactions

Experiment 8 - Double Displacement Reactions Experiment 8 - Double Displacement Reactions A double displacement reaction involves two ionic compounds that are dissolved in water. In a double displacement reaction, it appears as though the ions are

More information

An Introduction to Standards and Quality Control for the Laboratory Barbara Martin

An Introduction to Standards and Quality Control for the Laboratory Barbara Martin An Introduction to Standards and Quality Control for the Laboratory Barbara Martin Hach Company, 2002. All rights reserved. Lit. No. 2426 eac/rb 8/02 1ed In memory of Clifford C. Hach (1919-1990) inventor,

More information

4.0 EXPERIMENT ON DETERMINATION OF CHLORIDES

4.0 EXPERIMENT ON DETERMINATION OF CHLORIDES 4.0 EXPERIMENT ON DETERMINATION OF CHLORIDES Sl. No. Contents Preamble 4.1 Aim 4.2 Introduction 4.2.1 Environmental Significance 4.3 Principle 4.4 Materials Required 4.4.1 Apparatus Required 4.4.2 Chemicals

More information

A Visual Method for the Detection of Arsenic 0 to 500 µg/l By Dan Kroll R&D Chemist. Hach Co., Loveland Colorado

A Visual Method for the Detection of Arsenic 0 to 500 µg/l By Dan Kroll R&D Chemist. Hach Co., Loveland Colorado Introduction: Arsenic is a common contaminant of ground water that has been found to adversely effect human health at levels as low as 10 µg/l. The current USEPA maximum contaminant level (MCL) for arsenic

More information

Ion Exchange Determination of Na+ by Displacement and Zn 2+ Using Preconcentration. Reading: Harris pp. 641-647, 699

Ion Exchange Determination of Na+ by Displacement and Zn 2+ Using Preconcentration. Reading: Harris pp. 641-647, 699 Ion Exchange Determination of a+ by Displacement and Zn 2+ Using Preconcentration Reading: Harris pp. 641647, 699 Prelab Assignment: 1. The ph of a solution is 4.56. Determine the concentration and use

More information

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy Determining the Quantity of Iron in a Vitamin Tablet Computer 34 As biochemical research becomes more sophisticated, we are learning more about the role of metallic elements in the human body. For example,

More information

OXIDATION-REDUCTION TITRATIONS-Permanganometry

OXIDATION-REDUCTION TITRATIONS-Permanganometry Experiment No. Date OXIDATION-REDUCTION TITRATIONS-Permanganometry INTRODUCTION Potassium permanganate, KMnO 4, is probably the most widely used of all volumetric oxidizing agents. It is a powerful oxidant

More information

EXPERIMENT 5. Molecular Absorption Spectroscopy: Determination of Iron With 1,10-Phenanthroline

EXPERIMENT 5. Molecular Absorption Spectroscopy: Determination of Iron With 1,10-Phenanthroline EXPERIMENT 5 Molecular Absorption Spectroscopy: Determination of Iron With 1,10-Phenanthroline UNKNOWN Submit a clean, labeled 100-mL volumetric flask to the instructor so that your unknown iron solution

More information

Total Aerobic Bacteria, Yeasts and Molds

Total Aerobic Bacteria, Yeasts and Molds Total Aerobic Bacteria, Yeasts and Molds DOC316.53.01223 Total Aerobic Bacteria (Amber), Yeast and Mold (Red), Total Coliform (Red) and Disinfection Control (Purple) Paddle Testers Scope and application:

More information

Ion Selective Electrodes

Ion Selective Electrodes Ion Selective Electrodes OAKTON has a large selection of Ion Selective Electrodes () to suit a wide variety of applications. Each electrode has a typical response time of 20 to 30 seconds but will vary

More information

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. IgM ELISA For the quantitative determination of IgM in human serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calibrator

More information

Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07

Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3

More information

Name: Class: Date: 2 4 (aq)

Name: Class: Date: 2 4 (aq) Name: Class: Date: Unit 4 Practice Test Multiple Choice Identify the choice that best completes the statement or answers the question. 1) The balanced molecular equation for complete neutralization of

More information

Appendix D lists the Field Services Standard Operating Procedures. Appendix E lists the Biological Monitoring Standard Operating Procedures.

Appendix D lists the Field Services Standard Operating Procedures. Appendix E lists the Biological Monitoring Standard Operating Procedures. Page 16 of 87 3.3 Sample Collection, Storage and Preservation Figure 3 details required containers, sample volumes, preservation techniques, and holding times for proper sample collection. A discussion

More information

Rat Creatine Kinase MB isoenzyme,ck-mb ELISA Kit

Rat Creatine Kinase MB isoenzyme,ck-mb ELISA Kit Rat Creatine Kinase MB isoenzyme,ck-mb ELISA Kit Catalog No: E0479r 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

5.0 EXPERIMENT ON DETERMINATION OF TOTAL HARDNESS

5.0 EXPERIMENT ON DETERMINATION OF TOTAL HARDNESS 5.0 EXPERIMENT ON DETERMINATION OF TOTAL HARDNESS Sl. No. Contents Preamble 5.1 Aim 5.2 Introduction 5.2.1 Environmental Significance 5.3 Principle 5.4 Materials Required 5.4.1 Apparatus Required 5.4.2

More information

ph Alkalinity of Water

ph Alkalinity of Water ph Alkalinity of Water DOC316.52.93085 Based on ISO standard 9963-1:1994 ph-metric Titration 0.4 to 20 mmol/l of Total Alkalinity 1. Introduction Alkalinity of water is its acid-neutralizing capacity.

More information

ENCM 31073-Environmental Impact Assessment and Environmental Monitoring. Determination of nitrate and phosphate levels in well water/surface water

ENCM 31073-Environmental Impact Assessment and Environmental Monitoring. Determination of nitrate and phosphate levels in well water/surface water ENCM 31073-Environmental Impact Assessment and Environmental Monitoring ntalmonitoringenvironmental Monitoring - Practical Number 4 Determination of nitrate and phosphate levels in well water/surface water

More information

Environmental Water Testing: Surface Water, Groundwater, Hard Water, Wastewater, & Seawater

Environmental Water Testing: Surface Water, Groundwater, Hard Water, Wastewater, & Seawater Document: AND Sol Env 08 2013 Environmental Water Testing: Surface Water, Groundwater, Hard Water, Wastewater, & Seawater Matrix specific sample preparation and testing methods for environmental waters

More information

LAB PROCEDURE Alpha-Amino Nitrogen by NOPA

LAB PROCEDURE Alpha-Amino Nitrogen by NOPA LAB PROCEDURE Alpha-Amino Nitrogen by NOPA From: Method by Dr. Christian Butzke, U.C. Davis. The reagents have been changed slightly for better shelf life. In the original method, NaOH, boric acid, NAC,

More information

ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE

ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of total nitrogen in food and crude protein calculation (Kjeldahl method) Responsible person: Assoc.Prof. Ing.Kateřina Riddellová,

More information

Complexometric Titrations

Complexometric Titrations Complexometric Titrations Complexometric titrations are based on the formation of a soluble complex upon the reaction of the species titrated with the titrant. M + L ML In the module Chemistry 2 you were

More information

Experiment 5. Chemical Reactions A + X AX AX A + X A + BX AX + B AZ + BX AX + BZ

Experiment 5. Chemical Reactions A + X AX AX A + X A + BX AX + B AZ + BX AX + BZ Experiment 5 Chemical Reactions OBJECTIVES 1. To observe the various criteria that are used to indicate that a chemical reaction has occurred. 2. To convert word equations into balanced inorganic chemical

More information

Carolina s Solution Preparation Manual

Carolina s Solution Preparation Manual 84-1201 Carolina s Solution Preparation Manual Instructions Carolina Biological Supply Company has created this reference manual to enable you to prepare solutions. Although many types of solutions may

More information

Tutorial 4 SOLUTION STOICHIOMETRY. Solution stoichiometry calculations involve chemical reactions taking place in solution.

Tutorial 4 SOLUTION STOICHIOMETRY. Solution stoichiometry calculations involve chemical reactions taking place in solution. T-27 Tutorial 4 SOLUTION STOICHIOMETRY Solution stoichiometry calculations involve chemical reactions taking place in solution. Of the various methods of expressing solution concentration the most convenient

More information

Microbiological Testing of the Sawyer Mini Filter. 16 December 2013. Summary

Microbiological Testing of the Sawyer Mini Filter. 16 December 2013. Summary Microbiological Testing of the Sawyer Mini Filter 16 December 2013 Summary The Sawyer Mini Filter was tested for its ability to remove three microorganisms Raoultella terrigena, Bacillus subtilis, and

More information

Solubility Product Constants

Solubility Product Constants Solubility Product Constants PURPOSE To measure the solubility product constant (K sp ) of copper (II) iodate, Cu(IO 3 ) 2. GOALS 1 To measure the molar solubility of a sparingly soluble salt in water.

More information

Mouse IgM ELISA. Cat. No. KT-407 K-ASSAY. For the quantitative determination of IgM in mouse biological samples. For Research Use Only. 1 Rev.

Mouse IgM ELISA. Cat. No. KT-407 K-ASSAY. For the quantitative determination of IgM in mouse biological samples. For Research Use Only. 1 Rev. K-ASSAY Mouse IgM ELISA For the quantitative determination of IgM in mouse biological samples Cat. No. KT-407 For Research Use Only. 1 Rev. 072309 K-ASSAY PRODUCT INFORMATION Mouse IgM ELISA Cat. No. KT-407

More information

Chemistry: Chemical Equations

Chemistry: Chemical Equations Chemistry: Chemical Equations Write a balanced chemical equation for each word equation. Include the phase of each substance in the equation. Classify the reaction as synthesis, decomposition, single replacement,

More information

Chapter 16: Tests for ions and gases

Chapter 16: Tests for ions and gases The position of hydrogen in the reactivity series Hydrogen, although not a metal, is included in the reactivity series because it, like metals, can be displaced from aqueous solution, only this time the

More information

The Determination of an Equilibrium Constant

The Determination of an Equilibrium Constant The Determination of an Equilibrium Constant Computer 10 Chemical reactions occur to reach a state of equilibrium. The equilibrium state can be characterized by quantitatively defining its equilibrium

More information

Pocket Colorimeter II

Pocket Colorimeter II DOC022.53.80451 Pocket Colorimeter II User Manual 04/2014, Edition 1 Table of Contents Section 1 Specifications...3 Section 2 General information...5 2.1 Safety information...5 2.1.1 Use of hazard information...5

More information

HS 1003 Part 2 HS 1003 Heavy Metals Test

HS 1003 Part 2 HS 1003 Heavy Metals Test HS 1003 Heavy Metals Test 1. Purpose This test method is used to analyse the heavy metal content in an aliquot portion of stabilised hot acetic acid extract by Atomic Absorption Spectroscopy (AAS). Note:

More information

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in

More information

Odor (Threshold Odor, Consistent Series)

Odor (Threshold Odor, Consistent Series) METHOD #: 140.1 (Issued 1971) TITLE: ANALYTE: INSTRUMENTATION: Odor (Threshold Odor, Consistent Series) Odor N/A STORET No. 60 C: 00086 Room Temp: 00085 1.0 Scope and Application 1.1 This method is applicable

More information

ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND

ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND #3. Acid - Base Titrations 27 EXPERIMENT 3. ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND Carbonate Equilibria In this experiment a solution of hydrochloric

More information

Santa Monica College Chemistry 11

Santa Monica College Chemistry 11 Types of Reactions Objectives The objectives of this laboratory are as follows: To perform and observe the results of a variety of chemical reactions. To become familiar with the observable signs of chemical

More information

CHEMICAL FORMULAS AND FORMULA WEIGHT CALCULATIONS

CHEMICAL FORMULAS AND FORMULA WEIGHT CALCULATIONS CHEMICAL FORMULAS AND FORMULA WEIGHT CALCULATIONS 1. THE MEANING OF A CHEMICAL FORMULA A chemical formula is a shorthand method of representing the elements in a compound. The formula shows the formulas

More information

OBJECTIVE: To measure Chemical Oxygen Demand. BACKGROUND AND PRINCIPLE: SELECTION OF METHODS. Page 1

OBJECTIVE: To measure Chemical Oxygen Demand. BACKGROUND AND PRINCIPLE: SELECTION OF METHODS. Page 1 Page 1 Indian Institute of Technology Delhi Department of Civil Engineering CEL212 Environmental Engineering (Second Semester 2013-14) Experiment 5: Chemical Oxygen Demand OBJECTIVE: To measure Chemical

More information

Chemistry Post-Enrolment Worksheet

Chemistry Post-Enrolment Worksheet Name: Chemistry Post-Enrolment Worksheet The purpose of this worksheet is to get you to recap some of the fundamental concepts that you studied at GCSE and introduce some of the concepts that will be part

More information

Reagents also compatible in Hach

Reagents also compatible in Hach Reagents also compatible in Hach VARIO Powder Packs (PP) and Reagents for Photometry Test Range Reagent Liquid Reagent Tube Tests Powder Pack VARIO Aluminium Reagent, Set F20 Aluminium 0 0.22 mg/l Al VARIO

More information

Calculation of Molar Masses. Molar Mass. Solutions. Solutions

Calculation of Molar Masses. Molar Mass. Solutions. Solutions Molar Mass Molar mass = Mass in grams of one mole of any element, numerically equal to its atomic weight Molar mass of molecules can be determined from the chemical formula and molar masses of elements

More information

FT 2000. FIELD MEASUREMENT OF RESIDUAL CHLORINE

FT 2000. FIELD MEASUREMENT OF RESIDUAL CHLORINE FT 2000. FIELD MEASUREMENT OF RESIDUAL CHLORINE Use in conjunction with: FT 1000 General Field Testing and Measurement FQ 1000 Field Quality Control Requirements FS 1000 General Sampling Procedures FD

More information

XI. Methods of Analysis DETERMINATION OF POTASSIUM CARBONATE CALCULATIONS REAGENTS PROCEDURE

XI. Methods of Analysis DETERMINATION OF POTASSIUM CARBONATE CALCULATIONS REAGENTS PROCEDURE XI. NOTE: An automatic titrator may be utilized for ease of analysis, especially if this test is performed often. Contact Technical Service for further information concerning automatic titration. Methods

More information

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Catalog Number. CSB-E14405r For the quantitative determination of rat creatine kinase MM isoenzyme (CK-MM) concentrations in serum, plasma and tissue

More information

THE ACTIVITY OF LACTASE

THE ACTIVITY OF LACTASE THE ACTIVITY OF LACTASE Lab VIS-8 From Juniata College Science in Motion Enzymes are protein molecules which act to catalyze the chemical reactions in living things. These chemical reactions make up the

More information

Analytical Test Report

Analytical Test Report Analytical Test Report Customer: Address (City, State): Purchase Order: Report Number: Project Number: Date Received: Date of Report: Test Location: Boulder, CO. Assay: Part Number: Amino Acids by HPLC

More information

DRINKING WATER - LAB EXPERIMENTS. Coagulation and flocculation LAB EXPERIMENTS. Jartest

DRINKING WATER - LAB EXPERIMENTS. Coagulation and flocculation LAB EXPERIMENTS. Jartest DRINKING WATER - LAB EXPERIMENTS LAB EXPERIMENTS Coagulation and flocculation Jartest coagulation and flocculation - jartest lab experiments Framework This module explains the lab experiment on coagulation

More information

Nomenclature and Formulas of Ionic Compounds. Section I: Writing the Name from the Formula

Nomenclature and Formulas of Ionic Compounds. Section I: Writing the Name from the Formula Purpose: Theory: Nomenclature and Formulas of Ionic Compounds 1. To become familiar with the rules of chemical nomenclature, based on the classification of compounds. 2. To write the proper name of the

More information

Experiment 12- Classification of Matter Experiment

Experiment 12- Classification of Matter Experiment Experiment 12- Classification of Matter Experiment Matter can be classified into two groups: mixtures and pure substances. Mixtures are the most common form of matter and consist of mixtures of pure substances.

More information

SPIKE RECOVERY AND DETERMINING A METHOD DETECTION LIMIT Pamela Doolittle, University of Wisconsin Madison, pssemrad@wisc.edu 2014

SPIKE RECOVERY AND DETERMINING A METHOD DETECTION LIMIT Pamela Doolittle, University of Wisconsin Madison, pssemrad@wisc.edu 2014 SPIKE RECOVERY AND DETERMINING A METHOD DETECTION LIMIT Pamela Doolittle, University of Wisconsin Madison, pssemrad@wisc.edu 2014 This experiment explores quality assurance practices which are commonly

More information

SODIUM CARBOXYMETHYL CELLULOSE

SODIUM CARBOXYMETHYL CELLULOSE SODIUM CARBOXYMETHYL CELLULOSE Prepared at the 28th JECFA (1984), published in FNP 31/2 (1984) and in FNP 52 (1992). Metals and arsenic specifications revised at the 55 th JECFA (2000). An ADI not specified

More information

Magnesium (II), Sodium (I), Potassium (I), Ammonium (I)

Magnesium (II), Sodium (I), Potassium (I), Ammonium (I) s (The Soluble Group) Magnesium (II), Sodium (I), Potassium (I), Ammonium (I) The salts of the cations of group V, with few exceptions, are quite soluble, this accounts for the fact that there is no common

More information

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Catalog Number. CSB-E14405r For the quantitative determination of rat creatine kinase MM isoenzyme (CK-MM) concentrations in serum, plasma, tissue homogenates.

More information

HOMEWORK 4A. Definitions. Oxidation-Reduction Reactions. Questions

HOMEWORK 4A. Definitions. Oxidation-Reduction Reactions. Questions HOMEWORK 4A Oxidation-Reduction Reactions 1. Indicate whether a reaction will occur or not in each of following. Wtiring a balcnced equation is not necessary. (a) Magnesium metal is added to hydrochloric

More information

Inc. Wuhan. Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard (liquid) 2

Inc. Wuhan. Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard (liquid) 2 Uscn Life Science Inc. Wuhan Website: www.uscnk.com Phone: +86 27 84259552 Fax: +86 27 84259551 E-mail: uscnk@uscnk.com ELISA Kit for Human Prostaglandin E1(PG-E1) Instruction manual Cat. No.: E0904Hu

More information

Additional Lecture: TITRATION BASICS

Additional Lecture: TITRATION BASICS Additional Lecture: TITRATION BASICS 1 Definition and Applications Titration is the incremental addition of a reagent solution (called titrant) to the analyte until the reaction is complete Common applications:

More information

Preparation of frequently used solutions

Preparation of frequently used solutions Preparation of frequently used solutions Content 1. Diluting Concentrated Acids (Last Login: 08/08/2009) 2. Indicators (Last Login: 27/07/2009) 3. Standard Buffer Solutions (Last Login: 27/07/2009) 4.

More information

Introduction. The following definitions may help you better understand the components of the data report.

Introduction. The following definitions may help you better understand the components of the data report. Introduction This document is designed to help our clients understand the quality control requirements and limitations of data reporting. There are three sections to this document. The first section will

More information

Standard Methods for the Examination of Water and Wastewater

Standard Methods for the Examination of Water and Wastewater 2320 ALKALINITY*#(1) 2320 A. Introduction 1. Discussion Alkalinity of a water is its acid-neutralizing capacity. It is the sum of all the titratable bases. The measured value may vary significantly with

More information

PART I: MULTIPLE CHOICE (30 multiple choice questions. Each multiple choice question is worth 2 points)

PART I: MULTIPLE CHOICE (30 multiple choice questions. Each multiple choice question is worth 2 points) CHEMISTRY 123-07 Midterm #1 Answer key October 14, 2010 Statistics: Average: 74 p (74%); Highest: 97 p (95%); Lowest: 33 p (33%) Number of students performing at or above average: 67 (57%) Number of students

More information

EXPERIMENT 8: Activity Series (Single Displacement Reactions)

EXPERIMENT 8: Activity Series (Single Displacement Reactions) EPERIMENT 8: Activity Series (Single Displacement Reactions) PURPOSE a) Reactions of metals with acids and salt solutions b) Determine the activity of metals c) Write a balanced molecular equation, complete

More information

MTT Cell Proliferation Assay

MTT Cell Proliferation Assay ATCC 30-1010K Store at 4 C This product is intended for laboratory research purposes only. It is not intended for use in humans, animals or for diagnostics. Introduction Measurement of cell viability and

More information

2. DECOMPOSITION REACTION ( A couple have a heated argument and break up )

2. DECOMPOSITION REACTION ( A couple have a heated argument and break up ) TYPES OF CHEMICAL REACTIONS Most reactions can be classified into one of five categories by examining the types of reactants and products involved in the reaction. Knowing the types of reactions can help

More information

ATOMS. Multiple Choice Questions

ATOMS. Multiple Choice Questions Chapter 3 ATOMS AND MOLECULES Multiple Choice Questions 1. Which of the following correctly represents 360 g of water? (i) 2 moles of H 2 0 (ii) 20 moles of water (iii) 6.022 10 23 molecules of water (iv)

More information

Question Bank Electrolysis

Question Bank Electrolysis Question Bank Electrolysis 1. (a) What do you understand by the terms (i) electrolytes (ii) non-electrolytes? (b) Arrange electrolytes and non-electrolytes from the following substances (i) sugar solution

More information

HOW TO MAKE STANDARD SOLUTIONS FOR CHEMISTRY

HOW TO MAKE STANDARD SOLUTIONS FOR CHEMISTRY HOW TO MAKE STANDARD SOLUTIONS FOR CHEMISTRY Phillip Bigelow Chemists make two common types of "standard solutions": Molar solutions Normal solutions Both of these solutions are concentrations (or strengths

More information

The basic division explosives for forensic purposes is shown in Fig. 1

The basic division explosives for forensic purposes is shown in Fig. 1 Isolation, concentration and determination of post blast residua by liquid chromatography with DAD detector capillary electrophoresis with DAD detector Firearm fumes (gunshot residua, GSR) - various metallic

More information

Neutralizing an Acid and a Base

Neutralizing an Acid and a Base Balancing Act Teacher Information Objectives In this activity, students neutralize a base with an acid. Students determine the point of neutralization of an acid mixed with a base while they: Recognize

More information

Protocol: HPLC (amino acids)

Protocol: HPLC (amino acids) Page 1 of 6 1. Chemicals Composition M MW gram volume (ml) product code 0-pthaldialdehyde C 8H 6O 2 134.13 Sigma P0657 Perchloric acid HClO 4 3.5 100.46 Dilute 70% stock solution 1:1 with demi to obtain

More information

3 The Preparation of Buffers at Desired ph

3 The Preparation of Buffers at Desired ph 3 The Preparation of Buffers at Desired ph Objectives: To become familiar with operating a ph meter, and to learn how to use the Henderson-Hasselbalch equation to make buffer solutions at a desired ph

More information

Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA

Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA KAMIYA BIOMEDICAL COMPANY Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA For the quantitative determination of mouse CKMB in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-57681

More information

Factors Affecting Enzyme Activity

Factors Affecting Enzyme Activity INTRODUCTION Factors Affecting Enzyme Activity The chemical reactions occurring in living things are controlled by enzymes. An enzyme is a protein in the cell which lowers the activation energy of a catalyzed

More information