Recombinant Protein Expression Services

Transcription

1 Recombinant Protein Services High quality recombinant protein for your research GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ USA Toll-Free: Tel: Fax: Bacteria Yeast SBP141026

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3 System Selection Guide System Bacteria Advantages Relatively inexpensive Simple genetics Easy to manipulate Easy scale up Fast Disadvantages Lack of efficient post-translational modifications Codon usage issues Inclusion bodies Low yield and activity for some eukaryotic Difficult to express higher MW Protein Application Examples Small Scale Evaluation & Optimization PROTential TM Protein Evaluation & Optimization Evaluate whether your target protein expresses in your chosen system Identify the and conditions that gives you the most robust soluble of your target protein Identify the best system for your target protein PROTential TM Protein Evaluation & Optimization Name Service Type Price* Description Standard Protein Evaluation (SC1653-B) (SC1653-I) (SC1653-M) $280 $400 $ weeks 3-4 weeks 3-4 weeks Test 1 condition for soluble in a customer chosen bacterial strain Test 1 condition for soluble in Sf9, Sf21, S2 or Hi-5 cells Test 1 condition for soluble in CHO or 293 cells Yeast Comprehensive post-translational modifications Excellent method for the production of bioactive Good secretion Post translational modifications resemble mammalian system Suitable for toxic gene products Diverse post-translational modifications Low cost of culture media Industry-scale fermentation Long production time High media costs Protein yields relatively lower Long production time Relatively high media costs Improper glycosylation Excessive glycosylation Antigen Biochemical analyses Diagnostic studies Drug discovery & validation Functional assays Protein-protein interactions Structural biology Therapeutic protein Silver Gold Protein Optimization Protein Optimization All 3 Systems (SC1653-3S) (SC1667) (SC1668) $950 By Quote By Quote 3-4 weeks 2-3 weeks 4-8 weeks Deliverables Include Comprehensive data report with solubility information The best condition/matrix among tested (Silver & Gold packages only) Optimized (Gold package only) Test soluble 1 condition/ system (E.coli,, ); total 3 systems Test 8 different conditions Optimize growth temperature, media components & inducer concentrations Identify the best condition with your chosen vector and bacterial strain Test 48 different conditions Optimize growth temperatures, media components, inducer concentrations, promoters, host cell strains & fusion partners Robust, industry-first, high-throughput and solubility optimization matrix Standard Workflow Protein & Purification Streamlined Services Why use this service? PROTential TM allows you to seamlessly integrate protein along with your gene synthesis order to eliminate protein uncertainties and identify the best system for your target protein Verisign Secured Secure online submission of gene s Gene synthesis and cloning Protein and purification Multiple QC measuremetnts Fast delivery

4 Bacterial Services BacPower TM Guaranteed Bacterial Protein Target protein Tag Options Small tag (e.g., His) Large tag (e.g., GST) No Tag Amount (mg) Purity Options % 85% 90% 9-10 Endotoxin Level Options 1.0 EU/μg 0.1 EU/μg 0.01 EU/μg Price* $2,200 Why use BacPower TM Guaranteed Services? Zero-risk, best value packages that can be used for most target under 100 kda. Hassle-free service and lots of options to choose from we would recommend you try this first in case of an project. Moreover, you can get an instant quote online within seconds Services Transient in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript's transient mammalian protein service offers fast production and delivery of high quality recombinant and monoclonal antibodies at very flexible scale, from microgram to gram quantities. Transient Package MamPower TM mammalian protein (SC1259P) Target protein 8-12 $8,900 Price* Deliverables When to Use this Service Synthetic gene 3 mg protein at 85% purity When you need hassle-free, high quality, synthetic gene and guaranteed transient mammalian protein BacPower TM Customized Bacterial Protein Deliverables Delivery Time MamPower TM mammalian antibody (SC1259A) Target antibody 8-12 $8,499 Synthetic gene 50 mg antibody When you need hassle-free, high quality, synthetic gene and guaranteed transient mammalian antibody Target (DNA or protein)/dna template/ Pure, recombinant protein as specified by the customer Depending on project complexity, it can be as short as 6 weeks Why use BacPower TM Customized Services? Best for higher difficulty targets like membrane, toxins, ion channel etc. Also, ideal if you have unique experimental requirements [example special protocols, your own recommendations, your DNA samples instead of gene synthesis etc.] Customizable transient mammalian protein Target (DNA or protein)/dna template/ Depending on project complexity, it can be as short as 8 weeks Starts at $2,000 Protein as requested Flexible package when you need custom-scale services. Ideal if you have specific instructions or special preferences [e.g., cell line, protein purity levels, tags, etc.] Fast gene-to-protein TM Industry s Fastest Protein Production Service Target protein Deliverables 3 mg protein, guaranteed COA including QC data Gene report (additional cost) Purity Options Delivery Time Why use Fast gene-to-protein TM Services? Best when you have a time-sensitive project and need protein fast for a milestone deadline, manuscript publication, grant renewal or simply to accelerate your research. By the way, contrary to what most people would think, this service does not cost a lot higher than the standard services! 75% 85% 30 days We offer comprehensive stable cell line development capabilities that either begins with DNA provided by our customers or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Stable Package Steps Deliverables Description Gene synthesis & subcloning (optional) 4 Target gene(s) in puc57 & vector map Codon-optimization & gene synthesis Subcloning into mammalian cell vector Plasmid maxiprep for mammalian FragPower TM Guaranteed Antibody Fragments Target fragment amino acid Fragments System Price* scfv V HH /V H Fab $2,399 $3, Purity Options 90% Deliverables Antibody Fragment in buffered solution with the specified amount & purity Guaranteed Optimized gene report Stable bioproduction cell line (SC1503) Target (DNA or protein)/dna template/ Transient evaluation Stable cell line development 2-3 report Top 3 stable clones (5 clones in case of SC1557) Transfect cells with s using small scale system Detect levels by ELISA and/or WB Stable transfection and screening single cell clones MTX amplification and screening Subcloning of top 3 clones Screening subclones with highest productivity Stability test for 10 passages Batch culture to evaluate final Research cell bank preparation Why use FragPower TM Guaranteed Antibody Fragment Services? Excellent alternative to full length recombinant antibodies for research and diagnostic applications that require full antigen-binding capacity Advanced bioproduction cell line (SC1557) Process development 8-10 Comprehensive report Medium selection Fed-batch process development Physical compatibility experiment Feed supplementation test

5 Services Harness the superior power of insect cell using our Power TM and BacuVance TM proprietary technologies. Get higher quality protein with improved solubility and the desired post-translational modifications. Also suitable for of cell-toxic products. Power TM Guaranteed Package Price* Deliverables Large Scale Protein Production GenScript has the state-of-the-art fermentation/bioreactor equipment and expertise for large scale product recovery. Our versatile and reliable protein platforms include fermenters, wave mixers, and bioreactors, which accommodate different scales of protein production, from milligrams to a few hundreds grams. Large Scale Protein Production Capabilities System Capability Description Power TM Guaranteed Service BacuVance TM Custom Target protein $3,950 1 mg tagged protein COA report Bacteria Fermenter 2,000 L Transformed bacterial culture for large-scale fermentation, up to 2,000 L homogeneous recombinant, over 98% pure, for crystallization, and partially purified recombinant, over 70% pure large quantities of protein, up to several grams Package Steps Deliverables Description Gene synthesis & subcloning (optional) 2 Construct report Subcloning into one or more vectors WaveMixer 500 L one single system for the production of final homogeneous recombinant ; from gene synthesis to ; for orders of up to 100 mg Permits transient of customers' of interest, up to 10 mg in 10 days conditioned media and cell pellets from stable cell lines, up to 500 L, using a wave mixer system BacuVance TM custom insect Yeast Services 7 Target / DNA template/ Virus generation Pilot & purification evaluation Process development YeastHIGH TM Standard Yeast Target /DNA template/ Purified ml, 10 7 pfu/ml virus stock Comprehensive report Purified Generation of recombinant Bacmid DNA Transfection of insect cell with recombinant Bacmid DNA Generation of P1 stock (Low titer), P2 stock (High titer), and determination of virus titer by Quantitative-PCR Verify the protein for P1 and P2 generation by western blot with anti-his/gst or other antibody Infection of insect cells with P2 stock One-step affinity purification SDS-PAGE gel and Western blot 1 L insect cell and purification One-step purification QC by SDS-PAGE & Western blot Steps Deliverables Description Price* Gene synthesis & subcloning (optional) & evaluation Pilot & purification Large scale protein production & purification (optional) Construct report data Yeast paste/ Purified Subcloning into appropriate vector Plasmid maxi preparation Construct linearization Transformation of linear into appropriate host Confirmation of positive transformants via PCR confirmation SDS-PAGE and/or Western blot 1 L yeast culture One-step purification or additional purification to reach purity of 90% or higher QC by SDS-PAGE, Western blot, and MALDI-TOF (optional) Large-scale production up to 2,000 L (Employs fermenters and TFF large-scale filtration system) Deliverables available at >90% purity or higher Tag removal Starting at $2,900 Yeast WaveMixer Fermenter 100 L 2,000 L Other Protein Services High Throughput Variants Powered by our HTP gene synthesis and mutagenesis capabilities, we offer a unique, fully-flexible, small-scale HTP protein production service. Service includes gene synthesis, mutagenesis (if required) and protein production of up to 1,000 s in only 30 days. Deliverables include, files, QC certificate and affinity purified protein [ μg/well]. Chemical Protein Synthesis Our Chemical Protein Synthesis services use proprietary segment condensation and advanced peptide synthesis technologies to produce functional with 95% purity. This is an excellent approach to produce aggregation-prone, unstable and/or toxic. GLP-Compliant Bioprocess Services GenScript provides GLP-Compliant Bioprocess Services to produce recombinant and therapeutic antibodies for your in vivo studies, helping you smoothly integrate your results for manufacturing and regulatory application. High titer recombinant baculovirus stock carrying the customer's gene, up to 10 9 pfu/ml homogeneous recombinant, over 98% pure, for crystallization partially purified recombinant, over 70% pure a large scale protein production option, up to few hundred milligrams, using insect cells and a wave mixer system Yeast culture up to 2,000 L for large-scale fermentation homogeneous recombinant, over 98% pure, for crystallization partially purified recombinant, over 70% pure large quantities of protein, up to grams Structural Biology Services Our custom, CrystalPro TM Gene-to-Structure, structural biology services offer comprehensive services for structural biology, including high purity protein preparation, fragment-based crystallographic screening, crystallization and structure determination taking you from gene synthesis to crystallization/co-crystallization and structure determination. Process Development GenScript provides custom services for optimizing growth and production media for target cell lines, defining feeding strategies, studying cell line stability, and increasing production. We also offer a thorough analysis of growth rate, sub cultivation ratios, cell mass, and other parameters. Downstream process development includes protein purification and modification processes development and analytical assay development. Other Services Our other, high quality service offerings include Endotoxin removal, protein purification and characterization, protein refolding, etc. 8

6 Our Technology Examples Our Citations Record OptimumGene TM FoldArt TM Proprietary gene design and codon optimization technology produces over 10,000 candidate s during one round of analysis alone A complex sorting matrix then selects the best for each specific customized request The algorithm optimizes a variety of parameters that are critical to transcription, translation and protein folding and eliminates any codon bias Gives you significant increase in protein We have optimized over 50,000 s in all major systems High levels of protein results in inclusion body formation FoldArt TM is our proprietary in vitro solubilization & refolding platform to recover pure protein from inclusion bodies It includes high-hydrostatic-pressure techniques, small-molecule additives, and column-refolding methods to give you the soluble protein you desire at high purity GenScript is proud to serve scientists in over 80 countries, to become the most cited CRO in the world. Please visit for our entire 10,000+ scientific publications and use the search function on page to find publications citing a spectrum of GenScript protein services, including Bacterial services services services Yeast services Structural biology services ToxinEraser TM Solubility Tags Endotoxin removal is necessary for several downstream recombinant protein applications but the process can be challenging GenScript s proprietary ToxinEraser TM, endotoxin removal technology is based on the affinity matrix of our modified polymyxin B Get more out of your recombinant protein and increase your application-success-rate by using ToxinEraser TM Our Production Scientists have developed a variety of vectors with unique solubility tags for recombinant soluble protein production With over 95% success rate for projects, we can guarantee soluble protein delivery even when others fail Why Choose GenScript Protein Services Quality Speed Quotations & Ordering Information Quotations & Ordering Method How? Services Applicable Phone (or) All Protein services Fax (or) All Protein services protein@genscript.com All Protein services that have word-based quotation inquiry forms Online Inquiry By clicking on Get a Quote Now and following the on-screen instructions Found on many service pages Price Expertise Online Direct By clicking on Get a Quote Now of the respective page and following the on-screen instructions PROTential TM BacPower TM How to Order Support Submit quotation request by , fax or online quotation system Our Technical Account Manager will contact you within 24 hours to finalize quote You review and approve final order details and price You provide credit card/po information and order is placed Project is initiated immediately Our Project Manager will be in contact with you to provide updates * Prices shown are subject to change. For the most accurate pricing information, please visit our website