XVII th PRACTICAL COURSE ON BASIC STEM CELL LABORATORY TECHNIQUES AND MOLECULAR BIOLOGY APPLICATIONS SEPTEMBER 24-28, 2012 KOU-KOGEM

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1 XVII th PRACTICAL COURSE ON BASIC STEM CELL LABORATORY TECHNIQUES AND MOLECULAR BIOLOGY APPLICATIONS SEPTEMBER 24-28, 2012 KOU-KOGEM CHAIRMAN: PROF.DR. ERDAL KARAÖZ SCIENTIFIC ORGANIZATION COMMITTEE PROF.DR. ERTAN URAL ASSOC.PROF.DR MURAT KASAP ASSOC.PROF.DR GÜRALP ÖZKOÇ ASSIST.PROF.DR AYLA EKER SARIBOYACI ASSIST.PROF.DR. GÜLÇİN GACAR ASSIST.PROF.DR. GÖKHAN DURUKSU DR. GÜRLER AKPINAR MSc. AYÇA AKSOY MSc. Z. SEDA ÜNAL MSc. ÖZLEM SAĞLAM MSc. GÜLAY ERMAN Bio. CANSU SUBAŞI Lab. ALPASLAN OKÇU SPONSORS:

2 NUMBER OF PARTICIPANTS: LOCATION: Limited to 20 (participants will be separated into 2 and 3 groups) KOU-CENTER FOR STEM CELL AND GENE THERAPIES RESEARCH AND PRACTICE, SCHOOL OF MEDICINE, UMUTTEPE CAMPUS, IZMIT/KOCAELI / TURKEY KOU-KÖK HÜCRE VE GEN TEDAVİLERİ ARAŞTIRMA VE UYGULAMA MERKEZİ, TIP FAK. MORFOLOJİ BİNASI, UMUTTEPE YERLEŞKESİ, İZMİT/KOCAELİ ACCOMMODATION: PASHA PALAS HOTEL (4 STAR) (TEL ) COURSE FEE: Academic members, Specialist : 1500 TL Assistant, Biologist, Student : 1250 TL Representative of firms : 1500 TL Services included in the registartion fees; Course program and book, name tag, certificate, symposium bag, gala dinner, tea-coffee services and lunches during the Course PREREQUISITE: CERTIFICATE: All life science researchers and students can participate our course Successful participants are issued with a certificate by Kocaeli University KOGEM and Continuing Education Centre (bilingual/ Eng.-Tur.). An additional Flow-Cytometry training certificate will be issued by BD compony. ADMISSION: CONTACT: Please contact by (ekaraoz@hotmail.com) Prof.Dr. Erdal Karaöz Kocaeli Üniversitesi, Kök Hücre ve Gen Tedavileri Araştırma ve Uygulama Merkezi. Tıp Fakültesi Morfoloji Binası, Kat.1. Umuttepe Yerleşkesi, Üçtepeler/İzmit/Kocaeli Tel: ekaraoz@hotmail.com

3 COURSE PROGRAM DAY MONDAY : Registration : Introductory Lecture Prof.Dr. E. Karaöz : Stem Cell Biology and Mesenchymal Prof.Dr. E. Karaöz Stem Cells (MSCs) : Technical Properties of the Stem Cell Culture Assist.Prof.Dr. A.E. Sarıboyacı Lab.and Aseptic Conditions and Basic Stem Cell Laboratory Techniques: Primary, suspension, monolayer and 3D cultures 11:30-12:00 : Isolation of stem cells from bone marrow and Prof.Dr. E. Karaöz adipose tissue for clinical applications : Isolation techniques of rat MSCs from bone marrow and adipose tissue Lab. Alpaslan okçu : HANDS ON APPLICATION (KÖGEM/STEM CELL LABS) Isolation and culture of MSCs from rat bone marrow Isolation and culture of MSCs from human adipose tissue Isolation and culture of MSCs from human peripheral/ umbilical cord blood Isolation techniques of human and rodent MSCs from peripheral/cord blood/bone marrow/compact bone- negative/positive selection; EasySep, RosetteSep, RoboSep, StemSep. (E.KARAÖZ, A.AKSOY, A.OKÇU)

4 DAY TUESDAY : Subculturing, Cell Counting Techniques MSc.A.Okçu and Cryopreservation techniques : Characterization of MSCs and Evaluation of Assist.Prof.Dr.G.Gacar Apoptosis by Flow Cytometry : Cell cycle by Flow Cytometry Bio. G.Erman : ELISA applications in stem cell studies MSc. Z.S. Ünal : Lunch Break : HANDS ON APPLICATION (KÖGEM/CELL CULTURE LABS) Sub-culture, cell counting techniques, cryopreservation (freeze-thaw) techniques : APPLICATION (KÖGEM/ FLOW-CYTOMETRY LAB.) Flow cytometry practical applications (immunophenotypic analysis of MSCs from human adipose tissue and apoptosis detection through Annexin V) (G.GACAR, G.ERMAN) : APPLICATION (KÖGEM/STEM CELL LAB II) ELISA/ Insulin detection (Z.S. ÜNAL)

5 DAY WEDNESDAY : Western Blotting Assoc.Prof.Dr. M. Kasap : Cell Proliferation Index and Viability Tests (MTT,XTT,WST1) MSc. A. Aksoy : Tecan microplate readers and applications Bio. Esra Ada 11:20-12:00 : Stem Cells and Proteomics Dr. G. Akpınar : Telomeraze Enzyme Activity and Telomere Length Dr. G. Duruksu : APPLICATION (KOGEM/MOLECULAR BIOLOGY LAB I and II) Aplication and Assesment of MTT and WST Tests (A.AKSOY, A.OKÇU) Tecan microplate readers and applications (E.ADA, A.AKSOY, A.OKÇU) : APPLICATION (KOGEM/MOLECULAR BIOLOGY LAB I and II) DAY THURSDAY Protein isolation SDS-PAGE and Western blotting (M.KASAP, G.DURUKSU, G.AKPINAR, Z.S.ÜNAL, Ö.SAĞLAM,) Introduction of proteomix lab. (M.KASAP, G.AKPINAR) : Stem cell- Gene transfer tecniques Assist.Prof.Dr. G.Duruksu : RNA Isolation, cdna Synthesis and PCR Assist.Prof.Dr. G. Gacar : Gel Electrophoresis, Imaging Systems and Analyzing MSc. Ö. Sağlam : Real Time PCR Bio. B.Kanaçanlı :Co-culture tecniques Assist.Prof.Dr. G.Duruksu : APPLICATION (KÖGEM/ MOLECULAR BIOLOGY LAB I and II) RNA isolation and cdna synthesis, PCR and analysis of PCR products Agarose gel elektrophoretic analyses of RNA and DNA (G.GACAR, G.DURUKSU, Z.S. ÜNAL, Ö.SAĞLAM) : APPLICATION (KÖGEM/ STEM CELL LAB II) Gene transfer applications (Transformation of GFP gene into MSCs and screening under fluoresence microscope) (G.DURUKSU) Real Time PCR application (Z.S. ÜNAL, Ö.SAĞLAM, B:KANAÇANLI) : UYGULAMA (KÖGEM/ STEM CELL LAB I) Co-culture tecniques ( E.KARAÖZ, A.AKSOY,A.OKÇU)

6 GALA DINNER DAY FRIDAY : In-vitro Differentiation Techniques MSc. A. Aksoy : Overview of Tissue Engineering Applications Assist.Prof.Dr.G. Özkoç : Immunohistochemical techniques in cell culture Bio. C.Subaşı : APPLICATION (KÖGEM/CELL CULTURE LABS) Application of in-vitro differentiation techniques Seeding of MSCs on scaffolds in tissue engineering studies : CERTIFICATE CEREMONY