Custom TaqMan Array Cards

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1 PRODUCT ULLETIN Custom TaqMan Array Cards Custom TaqMan Array Cards Validate microarray hits quickly and economically Standardize screening of gene sets across multiple samples and laboratories Create the perfect TaqMan Array Card by incorporating your own specific design needs Load wells in typically less than five minutes without liquid-handling robots or multichannel pipettors The Custom TaqMan Array Card is a -well microfluidic card that enables you to perform simultaneous realtime PCR reactions without the need for liquid-handling robots or multi-channel pipettors to load samples. This highly flexible card enables to samples to be run in parallel against to TaqMan Gene Expression Assay targets (including a manufacturing control). The assays are preloaded into each well of the card and are ready for use immediately upon delivery. Choose from over 0,000 inventoried TaqMan Gene Expression Assays designed for human, mouse, and rat genes to be loaded onto a Custom TaqMan Array Card (Figure ). For added flexibility, you can contact your local Life Technologies sales representative to order cards loaded with Predesigned made-toorder or Custom TaqMan Gene Expression Assays. The Custom TaqMan Array Card is designed for use on the state-of-the-art QuantStudio K Flex, or ViiA Real-Time PCR Systems when equipped with TaqMan Array Card blocks. Ultimate microarray validation tool TaqMan Array Cards are exactly the right tool for validating the tens or hundreds of hits generated from microarrays because they can be customized to include up to of those hits in one easy-to-use card. Using individual TaqMan Assays, or even SYR dye based assays, to look at,, or targets can quickly become unmanageable and expensive. TaqMan Array Cards enable researchers to accomplish the validation necessary to arrive at the right answer easily and affordably. Ideal screening technology TaqMan Array Cards are ideal for screening biomarkers and toxicology panels, and for analyzing pathways, target classes, and complete disease sets. ecause TaqMan Array Cards do not require liquid-handling robots for loading, you get standardized results with low variability across multiple users and laboratories. Plus, TaqMan Gene Expression Assays the gold standard of specificity and sensitivity in real-time PCR are preloaded onto the TaqMan Array Card, enabling reliable performance. Figure. Custom TaqMan Array Cards can be designed by researchers using any of over 0,000 inventoried TaqMan Gene Expression Assays the gold standard in real-time PCR specificity and sensitivity.

2 Create the perfect card You simply select TaqMan Gene Expression Assays and the optimal Custom TaqMan Array Card format for your experiment, and we will deliver your TaqMan Array Card preloaded with your selected assays. Choose to target assays from our collection of inventoried TaqMan Gene Expression Assays covering human, mouse, and rat genes. Ordering is easy with the online Custom TaqMan Array Card configuration tool, which helps you find and select genes and assays. Custom TaqMan Array Cards are available in 0 different formats covering,,,,,, ( choices),, and assays. A Designing a Custom TaqMan Array Card Customizing a TaqMan Array card through the Life Technologies website at lifetechnologies.com/arraycards is simple. Choose your array format and method for choosing TaqMan Gene Expression Assays, select a quantity, and place your order it is as easy as that. Online selection of TaqMan Assays is simple: search by using common keywords like Entrez gene symbols, RefSeq accession numbers, or UniGene IDs (Figure A). You can narrow your search results using the Target Class filters relevant to your research area (Figure ). For example, if you want to find TaqMan Assays for caspase genes in the area of apoptosis, select the Apoptosis target class filter and enter caspase in the Keyword Search box to return results for caspase genes (Figure C). C Additionally, TaqMan Gene Panels, which are predefined gene panels of TaqMan Gene Expression Assays corresponding to a biological pathway or target class, can be used to simplify configuration of a Custom TaqMan Array Card. The TaqMan Gene Panels are accessible through the online Custom TaqMan Array Card configurator. Figure. Customizing a TaqMan Array Card using our simple yet powerful search tool can be done quickly online at lifetechnologies.com/arraycards.

3 Custom TaqMan Array Cards Custom TaqMan Array Cards can be ordered in any of these ten format options. Format (Cat. No. ) unique assays + mandatory control unique samples Format (Cat. No. ) unique assays + mandatory control unique samples CTL CTL 0 0 A CTL CTL 0 0 CTL CTL 0 0 C CTL CTL 0 0 D CTL CTL 0 0 E CTL CTL 0 0 F CTL CTL 0 0 G CTL CTL 0 0 H CTL CTL 0 0 I CTL CTL 0 0 J CTL CTL 0 0 K CTL CTL 0 0 L CTL CTL 0 0 M CTL CTL 0 0 N CTL CTL 0 0 O CTL CTL 0 0 P 0 0 CTL CTL CTL A CTL CTL CTL C D CTL CTL CTL E F CTL CTL CTL G H CTL CTL CTL I J CTL CTL CTL K L CTL CTL CTL M N CTL CTL CTL O P 0 0 Format (Cat. No. ) unique assays + mandatory control unique samples 0 CTL 0 C 0 CTL 0 D 0 CTL 0 O 0 CTL 0 P CTL 0 CTL 0 CTL 0 CTL 0 CTL 0 CTL G M A E I K 0 CTL 0 CTL 0 CTL 0 CTL 0 CTL 0 CTL H N F J L Format (Cat. No. ) unique assays + mandatory control unique samples CTL CTL CTL A C D CTL CTL CTL E F G H CTL CTL CTL I J K L CTL CTL CTL M N O P 0 0 Format (Cat. No. ) unique assays + mandatory control unique samples 0 CTL 0 A CTL 0 C 0 0 D 0 CTL 0 E 0 0 F 0 CTL 0 G 0 0 H 0 CTL 0 I 0 0 J 0 CTL 0 K 0 0 L 0 CTL 0 M 0 0 N 0 CTL 0 O 0 0 P 0 0

4 Format (Cat. No. 00) unique assays + mandatory control unique samples CTL CTL CTL A C D E F G H CTL CTL CTL I J K L M N O P 0 0 Format a (Cat. No. ) unique assays + mandatory control unique samples 0 CTL 0 A C 0 0 D 0 CTL 0 E 0 0 F G 0 0 H 0 CTL 0 I 0 0 J K 0 0 L 0 CTL 0 M 0 0 N O 0 0 P 0 0 Format b Format (Cat. No. b ) (P/N ) unique assays + mandatory control unique assays + mandatory control unique samples unique samples 0 CTL 0 CTL 0 0 A 0 0 C 0 0 D E F G H 0 CTL 0 CTL 0 0 I J 0 0 K 0 0 L M N O 0 0 P 0 0 Format Format (Cat. No. 0) (P/N 0) unique assays unique + assays mandatory + mandatory control control unique sample unique sample 0 CTL 0 A 0 CTL C 0 0 D E F 0 0 G 0 0 H I J K L 0 0 M 0 0 N O P 0 0 Format (Cat. No. ) 0 unique assays + mandatory controls unique sample 0 CTL CTL 0 A 0 CTL CTL C D E F 0 0 G 0 0 H I 0 0 J K 0 0 L M N 0 0 O P 0 0

5 . Load. Spin. Seal. Run minutes to complete steps through Figure. The TaqMan Array Card streamlines the reaction setup process in four simple steps. Loading the array with your cdna sample and TaqMan Universal Master Mix II, then spinning and sealing the card, typically takes only five minutes. Predesigned gene signature cards are also available If you do not have the time to compose a list of assays, use one of our predesigned TaqMan Array Gene Signature Cards. TaqMan Array Gene Signature Cards contain select TaqMan Gene Expression Assays for focused gene sets for many important target classes and pathways. Genes in each of the arrays have been culled from pathway analysis tools, published review papers, and collaborator and customer input. Select from a variety of TaqMan Array Gene Signature Cards covering areas such as GPCRs, immune response, or protein kinases at our website at lifetechnologies.com/signaturecards. You can also use our Endogenous Control Arrays to assess which housekeeping genes are best for your specific study. Simple and straightforward loading Once you receive your TaqMan Array Card, using it is fast and simple. The TaqMan Array Card has eight sample-loading ports, each connecting to reaction wells. Simply combine ng of cdna sample with TaqMan Universal Master Mix II (for each port), pipette sample mix into the TaqMan Array Card, centrifuge briefly to fill the wells, and seal the card. Within minutes, your -well TaqMan Array Card is ready to run on the QuantStudio K Flex, ViiA, or 00HT Real-Time PCR System (Figure ). Imagine how much time and costs you can save by not having to set up individual reactions, as well as reducing the the risk of introducing variability. Accurate, quantitative gene expression results are available when thermal cycling is complete. The reduced number of pipetting steps streamlines the reaction setup process and eliminates the need for liquid-handling robotics. Applied iosystems real-time PCR instruments TaqMan Array Cards function seamlessly with the Applied iosystems QuantStudio K Flex, ViiA, and 00HT Real-Time PCR Systems. The new QuantStudio K Flex Real-Time PCR System offers unprecedented throughput, flexibility, and scalability and is also capable of running TaqMan OpenArray Plates. The ViiA Real-Time PCR System also features interchangeable blocks as well as filter combinations. The 00HT Fast Real-Time PCR System has been used to run TaqMan Array Cards to generate extensively published and trusted gene expression results. Service and support Highly trained Service Engineers are available to install the TaqMan Array Card block on your QuantStudio K Flex, or ViiA Real-Time PCR System and get you up and running quickly. In addition, our Technical Support group can assist with any questions you may have. For more information, contact your Life Technologies sales representative or distributor. Powerful data analysis Our DataAssist Software package enables rapid and comprehensive interpretation of Custom TaqMan Array Card results. DataAssist Software provides a filtering procedure for outlier removal, various normalization methods based on single or multiple genes, and relative quantification (RQ) analysis of gene expression through a combination of statistical analysis and interactive visualization. DataAssist Software can be downloaded for free at lifetechnologies.com/dataassist.

6 Ordering information Description No. of assays No. of samples per card Minumum order quantity Cat. No. replicate replicates replicates replicates Custom TaqMan Array Card (-well microfluidic card) Format + control 0 Format + control 0 Format + control 0 Format + control 0 Format + control 0 Format + control 0 00 Format a + control 0 Format b + control 0 Format + control 0 0 Format 0 + controls 0 TaqMan Array Card locks* QuantStudio K Flex TaqMan Array Card lock ViiA TaqMan Array Card lock 00HT TaqMan Array Card lock 0 * Each TaqMan Array Card lock includes sample block, TaqMan Array Card sealer, custom buckets and adaptors, Getting Started guide, and chemical installation kit. A compatible centrifuge and rotor are required, but are not supplied. Ask your local sales representative for a list of compatible centrifuge and rotor options. For more information and full terms of the TaqMan Assays QPCR Guarantee, visit lifetechnologies.com/taqmanguarantee. 0 Life Technologies Corporation. All rights resserved. The trademarks mentioned herin are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Printed in the USA. CO0 0