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2 USB Molecular Biology Reagents and Biochemicals Bringing to you ExoSap-IT Phosphorilated Antibodies and many other Life Sciences Reagents More than 7000 antibodies for transcription, epigenetics and cell signaling. BD Falcon Plastic and Consumables Lab Equipment and Plastic consumables Wide range of solutions for immunology The PCR Company Leader in Molecular Biology reagents Your Primary choice for transfection reagents Immunoassays and Antibodies Leader in Cellular Methabolism Leading the Way in Cancer Diagnostics Antibodies, Purified Antigens and Specialty Research Products You Can Depend On Microscopy, camera and software solutions Worldwide supplier of laboratory equipment Frigoriferi e Congelatori per Laboratorio

3 World leader in development of ELISpot products and technology and T-cell measurements Preparation kits for RNA, microrna, DNA and protein purification, clean-up and concentration More than Antribodies Recombinant Proteins, Cytokine Packages, Growth Factors & Cytokines and even more Primary and Secondary Antibodies, Fusion Proteins Leader in the development of products for the biomedical research Bilance da laboratorio, Filtri, Dispositivi per ultrafiltrazione, Membrane e saggi diagnostici, Membrane per blotting Enzyme Immunoassay, Immunohistochemistry, Antibodies Inhibitors & Antibodies and sirna Albumins, Collagenase & Neutral Protease, Dialysis, Electrophoresis, Enzymology, Ion Exchange Worldwide leader in Elisa Kits, Antuibodies and Lifescience Reagents Provider of antigens and antibodies to the research and diagnostics markets

4 Aurogene Cell Colture Media Biotium GelRed Alternativa non tossica all Etidio Bromuro Aurogene Agarose Low EEO Aurogene Blue X-Ray Films

5 ServaGel Cod. Descrizione Preparazione Sample RIPA Lysis Buffer, 10X 100ml Millipore 2900 Nuclear Extraction Kit 100 assays Millipore 2145 Compartment Protein Extraction Kit Millipore Bradford reagent, 5x Quant.500ML BCA Assay Kit Quant.1KIT (480 tests) Protease Inhibitor Mix M Quant.1VIAL 100X (for 100ml extract) Dithiothreitol Quant.25G Mercaptoethanol Quant.50ML Laemmli Sample Buffer (2x), for SDS PAGE Quant.20ML Triton(R) X-100 Quant.250ML Bromophenol Blue-Na-salt Quant.25G CUVETTE MONOUSO CONF. 100 PZ. cc. 1,5 Acrylamide Solution Preparazione SDS GEL Acrylamide/Bis Solution, 29:1 Quant.500ML Acrylamide/Bis Solution, 37.5:1 Quant.500ML Acrylamide/Bis Solution, 29:1 Quant.4x500ML (30%) Acrylamide/Bis Solution, 37.5:1 Quant.4x500ML (30%) Ammonium persulfate Quant.250G SDS Solution, 20 % Quant.1L N,N,N',N'-Tetramethyl-ethylenediamine Quant.25ML Tris(hydroxymethyl)aminomethane Quant.2,5KG Glycine Quant.5KG Corsa Elettroforetica/Protein Transfer SERVA Tris-Glycine/SDS electrophoresis buffer (10x) Quant.1L SERVA Tris-Glycine native electrophoresis buffer (10x) Quant.1L BIO HyperPAGE Prestained Protein Marker 500µl Morpholinoethane sulfonic acid Quant.1KG (MES) Morpholinopropane sulfonic acid Quant.500G (MOPS) Sodium chloride Quant.1KG BL Rotolo Nitrocellulosa Por. 0.2um 300mmx3m FT N 3MM Blotting Paper 100fogli 580x600mm Gel/Membrane Staining Coomassie(R) Brilliant Blue R 250 Quant.25G SERVA Blue R staining (2x) Kit Quant.1KIT SERVA Silver Staining Kit SDS PAGE Quant.1KIT SERVA Silver Staining Kit Native PAGE Quant.1KIT Amido Black 10 B Quant.25G Ponceau S solution for electrophoresis (0.2 %) Quant.500ML Silver nitrate Quant.25G Ethanol undenaturated absolute Quant.2,5L Immunoblotting SC-2325 Blotto, non-fat dry milk 500g (SantaCruz) LT TBS, 20X SOLUTION LT TBS, 20X SOLUTION LT TBS WITH TWEEN -20 (20X) LT TBS WITH TWEEN -20 (20X) Tween(R) 20 Quant.100ML HyperPAGE Marker AU1101 AU1102 XLS10,0500 P7042-5GA P7167-5GA ECL - Lastre - Sviluppo Blue X Ray film 13 X 18 cm lastre Blue X Ray film 18 X 24 cm lastre WESTAR Nova 2011 HRP Detection Substrate 500ml Kit Kodak autoradiography films GBX developer/replenisher 5 Gallons Kodak autoradiography films GBX fixer/replenisher 5 Gallons

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7 Novità Aurogene: Higher sensitivity use less antibody, load less sample, carry out shorter exposures Long-lasting signal never rush to image your blot again Convenience just point, spray, and expose to film WesternBright MCF fluorescent Western blotting kit allows the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a loading control alongside a protein of interest. Assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously. The WesternBright protocol saves time and money since there is no need to strip and re-probe a blot, no use of disposable film, and the blot can be imaged immediately, without drying. The fluorescent dyes provided with WesternBright MCF outperform Cy dyes and allow detection of low pg amounts of protein. WesternBright ECL detects equal amounts of protein with up to ten times less antibody. Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions shown, and detected with either WesternBright ECL or Pierce ECL (Thermo Scientific) according to the manufacturers instructions. The blots were imaged simultaneously on the same piece of film. WesternBright ECL produced the same signal with ten times less primary and five times less secondary antibody.

8 Aurogene ha deciso di premiare la tua fedeltà! Iscriviti al Programma «Punti Aurogene» inviando una mail a indicando in oggetto «Programma Punti Aurogene». Riceverai un codice personale da inserire negli ordini che effettuerai presso di noi. Ogni euro di imponibile speso corrisponderà all accumulo di un Punto. Colleziona i tuoi Punti e scegli come spenderli tra i prodotti da laboratorio che ti presentiamo. Concorre all accumulo dei Punti Aurogene qualsiasi prodotto ordinato. I Punti Aurogene devono essere spesi entro il

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11 Dr. Francesco Dadda Mobile: Dr. Francesco Lunardi Mobile: Dr. Andrea Modesti Mobile: Dr. Gabriele Giorgi PhD Mobile: Dr. Alessandro Giovenco PhD Mobile: Dr. Bruno Bianco Mobile: Dr. Davide D Alise Mobile: Calsystem Srl Phone: Organigramma Societario: Dr. Antonio Petrone, PhD Direttore Generale Mail: Mobile: Dr. Christian Oliviero, PhD Responsabile Vendite Mail: Mobile: Dr.ssa Gilda Franzè Resposabile Amministrativo Mail: Phone Contatti: Informazioni: Amministrazione: Assistenza Tecnica: Logistica: Commerciale: Tel. : ; Fax : Indirizzo : Via dei Salentini 19, Roma Website: Inoltra la tua richiesta di offerta a

Western Blotting. USA: proteintech@ptglab.com UK & Europe: europe@ptglab.com China: service@ptglab.com. www.ptglab.com

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