Botector. Biotechnological botryticide. Effective protection against Botrytis cinerea on grapes

Transcription

1 Botector Biotechnological botryticide Effective protection against Botrytis cinerea on grapes

2 Index: Botrytis cinerea 3 Active substance 4 Production 5 Technical information 6 Mode of Action 7 Resistance Management 8 Application 1 Miscibility 11 Field trials 12 Must and wine quality Summary and contacts 16 2

3 Botrytis cinerea Causal agent of grey mold, stem rot and berry infections on grapes Depending on year and variety: high losses in yield, premature harvest Risk of secondary infections (Penicillium, Aspergillus acetic acid rot) Negative influence on taste, flavor and color of must Negative influence on fermentation 3

4 Active substance: 2 isolates of Aureobasidium pullulans Eu-ascomycote with asexual, yeast-like reproducing cells (blastospores) Natural occurrence in the environment (soil, plant surface) Well adapted, resistant against drought and UV radiation, frugal Aureobasidium pullulans on an agar plate under the microscope 4

5 Production of Botector Stock solution Liquid fermentation Fluidized bed dryer Granules (WG) 5

6 Active substance: 5x1 9 cfu/g Aureobasidium pullulans DSM and DSM 1494 Wettable Granules (WG) Package size: 1,2 kg and,4 kg Shelf life from date of manufacture: at room temperature ( 2ºC) 12 months, at cold storage ( 8ºC) 24 months 6

7 Mode of Action: Antagonism competition for space and nutrients Aureobasidium pullulans pathogen bio-ferm bio-ferm bio-ferm 1. Micro scratches on the fruit surface represent natural entrances for the pathogen. The scratches are colonized by Aureobasidium pullulans immediately after application of Botector (Picture: Mendgen). 2. Due to the high proliferation rate of Aureobasidium pullulans the pathogen cannot infect the plant (Picture: Mendgen). 3. The microscratch is completely colonized with Aureobasidium pullulans. Botector acts as a natural shield which protects grape bunches from infection with Botrytis cinerea (Picture: Mendgen). 7

8 Resistances of fungicides at Botrytis cinerea Chemical fungicide: Selection of resistant strains leads to decrease of efficacy of chemical botryticide Botector: Botector prohibits the selection of resistance carriers Share of resistent strains: 2.5% Share of resistent strains: 13% Chemical botryticde: Efficacy 71% Share of resistent strains: 38% Chemical botryticide: Efficacy 8% All pathogens mulitply (x5) Botector: Efficacy 8% Share of resistent strains: 13% Botrytis cinerea (not resistent strain) Botrytis cinerea (resistent strain) 8

9 Dosage 4 g/ha into the bunch zone 3 preventive applications per season Water volume 3-5L Miscibility observe current information according compatibility: Keep a spraying interval of min. 3 days with products which are not compatible! Other: Spray during evening hours when temperature is low. 9

10 Application 3 applications at 4 different possible dates BBCH 68: end of flowering Against latent infections, depending on weather conditions BBCH 77: before bunch closure against stalk- and berry infections BBCH 85: berry softening against berry infections (natural resistance of grapes collapses) BBCH 85-89: during ripening against berry infections (most sensitive stage), depending on weather (Lorenz et al., 1994) IP strategy: Replacement of the last chemical treatment before the harvest. Additional to chemical treatments in the waiting period of chemical products 1

11 Miscibility Oidium: Topas, Legend, Vivando, Bayfidan, Profital*, Vitisan* Peronospora: Mildicut, Copper * (except Cueva), Frutogard* Grape moth: Insegar, Mimic, Runner, Spin Tor, Steward, Xen Tari* Leave fertilizer: different N-, Mg-, Ca- and micro nutrients *for organic production 11

12 Incidence (%) Severity (%) Field trials trials from Austria, Switzerland, Greece, France, Portugal, Spain, Hungary, Slovakia, Germany and Italy 2-3x Botector vs. 2x botryticide ,1 2 17, ,3 22, ,4 11,1 1 5 untreated chemical Botector untreated chemical Botector standard standard 12

13 UK Botector Botrytizid UK Botector Botrytizid UK Botector Botrytizid 3,9 3,7 3, ,6 6,6 6,4 8,4 85 8,5 8, Must quality: no influence Chemical must analysis Must density Oe Total titratable acid g/l Tartaric acid g/l Malic acid g/l Free amino nitrogen mg/l Ammonium nitrogen mg/l FTIR, 27-29, n=3x15, Austria 13

14 Oe Oe Must density % Oe (Spontaneous) fermentation: no influence Spontaneous fermentation, Micro vinification Botector - untreated - Botryticide days Spontaneous fermentation Grüner Veltliner, Krems days Spontaneous fermentation, slow (14 C) Chardonnay, Langenlois days Spontaneous fermentation, fast (18 C) Grüner Veltliner, Krems days Spontaneous fermentation (18 C) Grüner Veltliner, Krems

15 UK Botector Botrytizid UK Botector Botrytizid UK Botector Botrytizid UK Botector Botrytizid 2,9 2,6 3,1 4, 4, 4, 8, 7,9 3,3 8,2 2,3,48 11,6 11,7 11,6 3,2,46,46 Wine quality: no influence Chemical young wine analysis Alcohol g/l Residual sugar g/l Total titratable acid g/l Volatile acid g/l Tartaric acid g/l Malic acid g/l 4,5 4, 3,5 3, 2,5 2, 1,5 1,,5, ,6,5,4,3,2,1, FTIR 27-29, n=3x1, Austria 15

16 Botector High and reliable efficacy in the field No negative influence on the ripening No phytotoxic reaction, no spraying spots No negative influence on the wine fermentation and wine quality No pre harvest interval No chemical residues No development of resistances No endangerment for humans and animals Sales contact: Mihailo Martinovic Mobile: Otto Kicker Mobile: