Why Cycle Control?" Manipulating Ovulation and Estrous Synchronization" Manipulating Ovulation" Cattle" Principle of PGF 2α Use"
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1 Why Cycle Control?" Manipulating Ovulation and Estrous Synchronization" John Parrish 1. Group females for parturition: " a) Decrease labor, calving period Reduce calving season" b) More uniform weaning weights. 2. Reduce or eliminate estrus detection. 3. Needed for artificial insemination: " Manipulating Hormonal induction of ovulation" PGF 2α " " Progestins" Superovulation" FSH" ecg" Cattle" Principle of PGF 2α Use" Estrous Cycle in the Bovine Regress active corpus luteum" Only effective on a day 5-17 CL" Not effective on days:" 1-4 (CL not responsive)" (CL already regressed)" Represents 1/3 of estrous cycle" Day After
2 Day After Day After Day After Day After Principle of Use" Works on CL Ovulation time dependent on status of follicular wave" Day After Luteinize or Ovulate a dominant follicle" Terminates the current follicular wave" Inhibin and Estradiol decrease" FSH increases to recruit a new follicular wave" No effect on old CL if present" A dominant follicle must be present" Generally followed by PGF 2α to regress CL formed or old CL"
3 Principle of Use" Induce ovulation of a dominant follicle" Used for timed AI" No CL present" Dominant follicle must be present" Eliminate current follicular wave 7 Days " Luteolysis Ovulates dominant follicle Dairy - OK" Beef - Problematic" Dominance Selection Recruitment Day After Day After PGF 2α Day After Day After
4 AI PGF 2α Eliminate current follicular wave 7 Days " Luteolysis Ovulates dominant follicle Day After Use of Progestogens" Principle" Provides progesterone" Simulates a CL" Prevents ovulation" Has no effect on the animalʼs CL (ie. normal lifespan)" Does not regress the CL!!!!" Remove after animalʼs CL regresses" Current or next follicular wave will ovulate!!!!" Occurs 2 5 days latter" May use PGF 2α to regress animalʼs CL " Administration" Injection, Feed (MGA), Implant, Control Internal Drug Release (CIDR) " Normal Cycle Day After Normal Cycle CIDR CIDR Prog." CL Prog." CL Prog." 26" Day After 26" Day After
5 Co-Synch + CIDR" " " PGF 2α" 0" 7" 10" Treatment Day" Pre-synchronization" 2 PGF 2α injections 14 days apart" 2 nd shot is days before synchronization program" Presynch" Double ovsynch" Same as ovsynch" 2 nd is 7 days before 1 st ovsynch " PG-3-G" PGF 2α, wait 3 days,, wait 7 days before synchronization program" Progestens Effect on " From C.L." Superovulation" 0" 17" 21" Follicular Wave" Follicular Wave" Follicular Wave" Stimulating Follicular Development" First Follicular" Wave" ecg or FSH" " From C.L." PGF 2α " 10-12" ecg or FSH" 17" Multiple" Ovulations"." Sheep" Progestin" CIDR" In season - works alone" Out of season - requires ecg" Prostaglandin" Only works in season" Lights - decrease day length" Melatonin - give orally or IM"
6 Swine" Prostaglandin " Not of practical " Only effect days 12-17" Progestins - Altrenogest (Matrix)" MGA causes ovarian cysts" Regumate (oral) for 18 days" Cost is $5 - $10/day" Wean Piglets" Puberty Induction - PG600 (ecg + HCG)" Equine" Prostaglandin" Mare CL more sensitive than cowʼs" Only effective in season" HCG ovulation induction, >35 mm follicle" Progestins" Altrenogest (Regumate)" Only effective in season" Reduce estrus behavior in competition animals" Light" 16 hrs day light for days."
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