Diagnostic Parasitology practical spot book (Cestodes) Page 11 of 21

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1 Diagnostic Parasitology practical spot book (Cestodes) Page 11 of 21 Tapeworms Slide Number 031 Taenia saginata (gravid segment) - Longer than broad (2x0.7cm) - Marginal common genital pore - Uterus, with lateral branches, (average 18) on each side.

2 Diagnostic Parasitology practical spot book (Cestodes) Page 12 of 21 Tapeworms Slide Number 032 Taenia solium (gravid segment): Resembles that of T. saginata but the number of the lateral uterine branches is less i.e (average 9) on each side.

3 Diagnostic Parasitology practical spot book (Cestodes) Page 13 of 21 Tapeworms Slide Number 033 Egg of Taenia spp.: Both eggs of T. saginata & T. solium are morphologically similar: - Shape: spherical, 30-40u in diameter - Shell: radially striated embryophore - Color: brownish - Content: mature (hexacanth larva)

4 Diagnostic Parasitology practical spot book (Cestodes) Page 14 of 21 Tapeworms Slide Number 034 Section in the muscle of an intermediate host (cattle or pig) showing a cysticercus (larva stage of: Taenia spp.): - Oval cyst, lined with germinal epithelium and surrounded by fibrous tissue reaction of the intermediate host. - Invaginated scolex (since in the section one can not detect the presence or absence of hooks, the species of the larva is not determined)

5 Diagnostic Parasitology practical spot book (Cestodes) Page 15 of 21 Tapeworms Slide Number 035 Jar showing adult Taenia spp.: - Flat, ribbon-shaped segmented worm - Measures several meters x 1cm - Mature segment - Square or slightly broader than long - Common genital pore: marginal, irregularly alternating - Gravid segment - Longer than broad - Uterus acquires lateral branches - Determination of the species depends on microscopical examination of the scolex (for rostellum & hooks) or gravid segment (for number of lateral uterine branches)

6 Diagnostic Parasitology practical spot book (Cestodes) Page 16 of 21 Tapeworms Slide Number 036 Hymenolepis nana (whole worm) - Size: 1-3cmx1mm - Scolex: Suckers: 4 true muscular ones Rostellum with one circles of hooks - Mature Segment: Broader than long (0.5x0.15mm) Common genital pore: marginal, unilateral (on same side) Testes: 3; one towards common genital pore and 2 close on the other side. - Ovary: between the testes: - Gravid segment: Broader than long - Uterus: sac full of eggs

7 Diagnostic Parasitology practical spot book (Cestodes) Page 17 of 21 Tapeworms Slide Number 037 Hymenolepis nana egg: - Shape: spherical, 30-50u is diameter - Shell: 2 coverings: outer than shell and inner embryophore with 2 polar thickenings, from each arises 4-8 filaments. - Color: translucent - Content: mature (hexacanth larva)

8 Diagnostic Parasitology practical spot book (Cestodes) Page 18 of 21 Tapeworms Slide Number 038 Echinococcus granulosus: (adult worm) - Size: mall (2-8mm) - Scolex: globular; 4 true muscular suckers; rostellum with 2 circles of hooks - Strobila: formed of 3 segments (one immature, one mature and one gravid) - Mature segment: - longer than broad; - marginal common genital pore; - one set of male and female genital organs (similar to those of Taenia spp.) - Gravid segment - longer than broad - uterus with lateral pouches

9 Diagnostic Parasitology practical spot book (Cestodes) Page 19 of 21 Tapeworms Slide Number 039 Egg of Echinococcus spp: Similar to egg of Taenia spp.

10 Diagnostic Parasitology practical spot book (Cestodes) Page 20 of 21 Tapeworms Slide Number 040 Section in an organ (liver or lung) containing hydatid cyst a. Wall consist of: i. Outer fibrous tissue (host tissue reaction) ii. Laminated cuticular layer (ectocyt) iii. Inner germinal layer (endocyt) b. Contents: i. Scolices ii. Brood capsules (sacs of germinal layer containing scolices) iii. Daughter cyst: exogenous & endogenous iv. Hydatid fluid

11 Diagnostic Parasitology practical spot book (Cestodes) Page 21 of 21 Tapeworms Slide Number 041 Hydatid sand: - Scolices: each carries 4 muscular suckers and invaginated rostellum with hooks - Brood capsules: sacs arising from the germinal layer in which scolices develop

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