Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Haematology and Blood Transfusion Laboratory Pathology Department Nethermayne Basildon SS16 5NL United Kingdom Contact: Kim Aston Tel: +44 (0) below Site activities performed away from the locations listed above: Location details Activity Address: Cardio Thoracic Centre Basildon Hospital Nethermayne Basildon SS16 5NL Local Contact David Green Tel: +44(0) (ext:4989) Satellite Blood Issue Fridge for storage of blood and thawed FFP only Address: Maternity Unit Basildon Hospital Nethermayne Basildon SS16 5NL Local Contact David Green Tel: +44(0) (ext:4989) Satellite Blood Issue Fridge for storage of blood and thawed FFP only Address: Haematology Day Unit Orsett Hospital Rowley Road Orsett Grays Essex RM16 3EU Local Contact David Green Tel: +44(0) (ext:4989) Satellite Blood Issue Fridge for storage of blood and thawed FFP only Assessment Manager: PW 1 of 6
2 DETAIL OF ACCREDITATION HUMAN BODY FLUIDS Blood Transfusion examination activities for the purposes of clinical diagnosis ABO group (forward and reverse) 1) Automated method; Ortho Innova card/column agglutination. (SOP BT-LP- 13) RhD type Red cell antibody screen Direct Antiglobulin Test (DAGT) for red cell antibodies Detection of red cell antibodies, as below Rhesus blood group antibodies: anti-c, anti-c, anti-d, anti-e, anti-e Kell system blood group antibodies: anti-k, anti-k, anti-kpa Kidd blood group antibodies: anti-jka, anti-jkb MNS blood group antibodies: anti-m, anti-n, anti-s, anti-s Duffy blood group antibodies: anti-fya, anti-fyb 2) Manual Method; Diamed/BioRad column agglutination and tube technique. (SOP BT-LP-50) Ortho Innova card/column agglutination (SOP BT-LP-54) Ortho Innova card card/column agglutination and Diamed/BioRad column agglutination. (SOP BT-LP- 16 and BT-LP-32) Automated method; Ortho Innova card/column agglutination. (SOP BT- LP-37) Manual Method; Diamed/BioRad column agglutination. (SOP BT-LP- 37) Assessment Manager: PW Page 2 of 6
3 Blood Transfusion activities for the purpose of clinical diagnosis (cont d) Serological cross match - serological compatibility testing between donor red cell antigens and patient plasma antibody. Manual method; Ortho Innova card/column agglutination and Diamed/BioRad column agglutination. (SOP BT-LP- 42) Detection and Estimation of Foetal Maternal Haemorrhage through quantification of foetal Hb in maternal blood Infectious Mononucleosis Screening / Glandular fever heterophile antibody testing Kleihauer test using acid elution followed by microscopy. (SOP BT- LP-65) Infectious mononucleosis screen using the Clearview Kit. (SOP BT- LP-53) HUMAN BODY FLUIDS Haematology (Coagulation) examination activities for the purpose of clinical diagnosis Coagulation Clotting Screen, (PT, INR, APTT, APTT ratio, Fibrinogen D-Dimer, thrombin time (TT) Coagulation Factor assays for II, V, VII, VIII, IX, X, XI and XII (SOP s HAEM-LP-91 and HAEM-LP-110) (SOP HAEM-LP-116) Manual APTT and PT/INR Visual determination of fibrin clot formation using a manual technique (SOP HAEM-LP-109, HAEM-LP-104) Coagulation determination of Low Molecular Weight Heparin (Also Danaparoid and Fondaparinux) (SOP HAEM-LP-117) Assessment Manager: PW Page 3 of 6
4 Bone marrow Haematology examination activities for the purpose of clinical diagnosis (cont d) Thrombophilia screen, consisting of: Protein C activity, Antithrombin III (ATIII) activity, Factor VIII level, Lupus anticoagulant screen Activated Protein C resistance (APCR) Full blood count; Haemoglobin, Red cell count, Platelet count, Haematocrit, White blood cell count (total) Differential white blood cell count (Neutrophil, Lymphocyte, Monocyte, Eosinophil, Basophil), NRBC count, Reticulocyte count, MCV, MCH, RDW Blood film staining and reporting of normal and abnormal morphologies including WBC Diff. Bone marrow slides staining and reporting of abnormal morphologies (SOP HAEM-LP-113) Sysmex XE5000s using fluorescence flow cytometry, electrical impedance, SLS-haemoglobin method, RBC pulse height detection method, sheath flow direct current (DC) detection method and calculated red cell parameters P HAEM-LP-73) Staining of peripheral blood smear using May Grunwald, Giemsa and Romanowsky stains using Sysmex SP-1000i (automated) and manual methods Examination by light microscopy. (SOP s HAEM-LP-84, HAEM-LP-12 and HAEM-LP-120) Manual Staining of bone marrow using May Grunwald, Giemsa and Romanowsky stains (HAEM-LP-120) Assessment Manager: PW Page 4 of 6
5 Bone marrow Urine Haematology examination activities for the purpose of clinical diagnosis (cont d) Detection of iron and ferritin Perl's Prussian Blue iron stain using a manual technique with examination by light microscopy. (SOP HAEM- LP-07) Erythrocyte sedimentation rate Sedimentation rate of red cells by a manual measurement. (SOP HAEM-LP-13) Detection and speciation of malaria parasites; Microscopic detection by Plasmodium vivax, Plasmodium falciparum Plasmodium ovale, Plasmodium malariae Plasmodium knowlesi. a) Thin film - May-Grunwald staining on methanol fixed blood smears b) Thin Film by Fields stain and c) Thick film by Giemsa MP staining kit. Detection of Plasmodium falciparum and the differential diagnosis of falciparum from other pan-specific Pasmodium species (vivax, ovale, malariae) Determination of Haemoglobin variants.. All with examination by light microscopy and using SOP HAE-LP-35 Immunodiagnostic testing by CareStart Malaria RAPYDTEST cards. (SOP HAE-LP-35) 1) Separation of haemoglobin variants by automated Capillary electrophoresis using Sebia CapillaryS Flex analyser SOP HAEM-LP-124 2) Manual separation by Acid Gel electrophoresis (SOP HAEM-LP-82) Assessment Manager: PW Page 5 of 6
6 Haematology examination activities for the purpose of clinical diagnosis (cont d) Detection of Haemoglobin S Manual sickle solubility assay using the Microgen Sickle Test kit. (SOP HAEM-LP-68) G6PD qualitative screen Fluorescence under UV light using Trinity Biotech screening reagents and manual technique SOP HAEM-LP-57) END Assessment Manager: PW Page 6 of 6
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
ccredited to Laboratory locations: Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK University Hospital NHS Foundation Trust General
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