MATERIAL SAFETY DATA SHEET

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1 MATERIAL SAFETY DATA SHEET 1 PRODUCT AND COMPANY IDENTIFICATION Product Name: itraq-115 Reagent Product No.: ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; Trade Name(s): ITRAQ REAGENT 4 plex 114; ITRAQ REAGENT 4 plex 115; ITRAQ REAGENT 4 plex 116; ITRAQ REAGENT 4 plex 117; ITRAQ REAGENT 8 plex 113; ITRAQ REAGENT 8 plex 114; ITRAQ REAGENT 8 plex 115; ITRAQ REAGENT 8 plex 116; ITRAQ REAGENT 8 plex 117; ITRAQ REAGENT 8 plex 118; ITRAQ REAGENT 8 plex 119; ITRAQ REAGENT 8 plex 121; ITRAQ REAGENT-114; ITRAQ REAGENT-115; ITRAQ REAGENT-116; ITRAQ REAGENT-117 Manufacturer Name: Applied Biosystems 850 Lincoln Centre Drive Foster City, CA USA Phone: (650) (USA) Emergency Telephone: (NORTH AMERICA); (INTERNATIONAL, REVERSE CHARGES) Intended Use: For Research Use Only, Not for Use in Diagnostic Procedures 2 HAZARDS IDENTIFICATION Emergency Overview Physical State: Liquid Color: No data available. Odor: No data available. WARNING! Harmful if inhaled, absorbed through skin, or swallowed. Causes eye irritation. May cause skin irritation. Flammable liquid and vapor. Potential Health Effects Inhalation: Harmful by inhalation. Vapors may cause drowsiness and dizziness. Eye Contact: Causes eye irritation. Exposed individuals may experience eye tearing, redness, and discomfort. NA ENGLISH MSDS 1 / 8

2 Skin Contact: Harmful if absorbed through skin. May cause skin irritation. Degreasing to skin. Ingestion: Harmful if swallowed. Chronic Health Effects: May cause damage to the liver and kidneys. Can cause cardiovascular system damage. Organic solvents may be absorbed into the body by inhalation and cause permanent damage to the nervous system, including the brain. Target Organ(s): Eye Skin Respiratory system Kidney Liver Cardiovascular system Central nervous system Potential Physical / Chemical Effects: FLAMMABLE. Heating will generate vapors which may form explosive vapor/air mixtures. OSHA Regulatory Status: This product is hazardous according to OSHA 29CFR Environment: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. 3 COMPOSITION / INFORMATION ON INGREDIENTS General Information: The product contains: Organic solvents. Chemical Name CAS-No. Concentration* Acetonitrile > 60% * All concentrations are percent by weight unless ingredient is a gas. Gas concentrations are in percent by volume. This chemical is hazardous according to OSHA/WHMIS criteria. 4 FIRST AID MEASURES General: Thermal burns: Flush with water immediately. While flushing, remove clothes which do not adhere to affected area. Call an ambulance. Continue flushing during transport to hospital. Inhalation: Move injured person into fresh air and keep person calm under observation. Get medical attention immediately. Eye Contact: Immediately flush with plenty of water for up to 15 minutes. Remove any contact lenses and open eyes wide apart. If irritation occurs, get medical assistance. Skin Contact: Remove contaminated clothes and rinse skin thoroughly with water for at least 15 minutes. Get medical attention promptly if symptoms occur after washing. Ingestion: Rinse mouth thoroughly with water and give large amounts of milk or water to people not unconscious. Get medical attention. 5 FIRE-FIGHTING MEASURES Extinguishing Media: Extinguish with foam, carbon dioxide, dry powder or water fog. NA ENGLISH MSDS 2 / 8

3 Unsuitable Extinguishing Media: Not applicable. Special Fire Fighting Procedures: Use standard firefighting procedures and consider the hazards of other involved materials. Cool containers exposed to heat with water spray and remove container, if no risk is involved. Unusual Fire & Explosion Hazards: During fire, gases hazardous to health may be formed. Solvent vapors may form explosive mixtures with air. Hazardous Combustion Products: Carbon Oxides, Hydrogen Cyanide, Nitrogen Oxides Protective Measures: Selection of respiratory protection for fire fighting: follow the general fire precautions indicated in the workplace. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Flammability Class: NFPA Rating Fire = 3. 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Extinguish all ignition sources. Avoid sparks, flames, heat and smoking. Ventilate. Avoid inhalation of vapors and contact with skin and eyes. Wear suitable protective clothing. See Section 8 of the MSDS for Personal Protective Equipment. Spill Cleanup Methods: Remove sources of ignition. Beware of the explosion danger. Absorb spillage with non-combustible, absorbent material. For waste disposal, see section 13 of the MSDS. Environmental Precautions: Avoid discharge into drains, water courses or onto the ground. Notification Procedures: Inform Authorities if large amounts are involved. 7 HANDLING AND STORAGE Handling: Mechanical ventilation or local exhaust ventilation is required. Use explosion proof electric equipment. Ground container and transfer equipment to eliminate static electric sparks. The product is flammable, and heating may generate vapors which may form explosive vapor/air mixtures. Do not smoke and do not spray near an open flame or other sources of ignition. Avoid inhalation of vapors and contact with skin and eyes. Wear approved safety goggles. Wear protective gloves and appropriate clothing to prevent skin contact. Observe good industrial hygiene practices. Storage: Follow rules for flammable liquids. Store in closed original container in a dry place. Keep away from heat, sparks and open flame. Keep away from food, drink and animal feeding stuffs. 8 EXPOSURE CONTROLS / PERSONAL PROTECTION Exposure Limits: Exposure far above the OEL may result in death. The effects might be delayed. Chemical Name Source Type Exposure Limits Notes Acetonitrile ACGIH TWA 20 ppm Skin Acetonitrile NIOSH Guide IDLH 500 ppm Acetonitrile US. OSHA TWA 40 ppm 70 mg/m³ NA ENGLISH MSDS 3 / 8

4 Table Z-1 Consult Canadian Provincial Regulations and/or Mexican Regulations on exposure limits, if applicable. MSDS PN: Engineering Controls: Provide adequate ventilation. Observe Occupational Exposure Limits and minimize the risk of inhalation of vapors. Use explosion-proof ventilation equipment. Provide easy access to water supply or an emergency shower. Respiratory Protection: If engineering controls do not maintain airborne concentrations below recommended exposure limits (where applicable) or to an acceptable level (in countries where exposure limits have not been established), an approved respirator must be worn. In the United States of America, if respirators are used, a program should be instituted to assure compliance with OSHA Standard 63 FR 1152, January 8, Seek advice from supervisor on the company's respiratory protection standards. Eye Protection: Wear approved safety goggles. Hand Protection: Wear protective gloves. Be aware that the liquid may penetrate the gloves. Frequent change is advisable. Suitable gloves can be recommended by the glove supplier. Skin Protection: Wear suitable protective clothing. Apron and long sleeves are recommended. Protective clothing should be chemical/oil resistant. Hygiene Measures: Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants. 9 PHYSICAL AND CHEMICAL PROPERTIES Color: No data available. Odor: No data available. Odor Threshold: No data available. Physical State: Liquid ph: Not applicable Melting Point: No data available. Freezing Point: No data available. Boiling Point: No data available. Flash Point: No data available. Evaporation Rate: No data available. Flammability Limit - Upper (%): No data available. Flammability Limit - Lower (%): No data available. Vapor Pressure: No data available. Vapor Density (Air=1): No data available. Specific Gravity: No data available. Solubility in Water: Miscible with water. Solubility (Other): No data available. Partition Coefficient (n-octanol/water): No data available. Autoignition Temperature: No data available. Decomposition Temperature: No data available. Viscosity: No data available. 10 STABILITY AND REACTIVITY NA ENGLISH MSDS 4 / 8

5 Stability: This product is stable under expected conditions of use. Conditions to Avoid: Heat, sparks, flames. May attack some plastics, rubber and coatings. Incompatible Materials: Strong oxidizing agents. Strong acids. Hazardous Decomposition Products: At Elevated Temperatures: Carbon Oxides, Hydrogen Cyanide, Nitrogen Oxides 11 TOXICOLOGICAL INFORMATION Specified Substance(s) Acute Toxicity: Component Chemical Name Acetonitrile Acetonitrile Acetonitrile Test Results Dermal LD50 (Rabbit): 395 mg/kg Inhalation LC50 (4 hour(s), Mouse): 1346 ppm (m) Oral LD50 (Rat): 1327 mg/kg Listed Carcinogens: Chemical Name IARC NTP OSHA ACGIH Acetonitrile Not Listed Not Listed Not Listed A4 IARC: 1 = Carcinogenic to Humans; 2A = Probably Carcinogenic to Humans; 2B = Possibly Carcinogenic to Humans; 3 = Not classifiable as to carcinogenicity to humans; 4 = Probably not carcinogenic to humans; Not listed = Not evaluated by IARC. ACGIH: A1 = Confirmed Human Carcinogen; A2 = Suspected Human Carcinogen; A3 = Confirmed Animal Carcinogen; A4 = Not classifiable as a human carcinogen; A5 = Not suspected to be a human carcinogen; Not listed = Not evaluated by ACGIH. Product Information Acute: Harmful if inhaled, absorbed through skin, or swallowed. Causes eye irritation. May cause skin irritation. Chronic Toxicity: May cause damage to the liver and kidneys. Can cause cardiovascular effects. Organic solvents may be absorbed into the body by inhalation and cause permanent damage to the nervous system, including the brain. 12 ECOLOGICAL INFORMATION Ecotoxicity: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. Mobility: The product contains organic solvents which will evaporate easily from all surfaces. Persistence and Degradability: No data available. Bioaccumulation Potential: No data available. 13 DISPOSAL CONSIDERATIONS NA ENGLISH MSDS 5 / 8

6 General Information: Dispose of waste and residues in accordance with local authority requirements. Disposal Methods: No specific disposal method required. Container: Since emptied containers retain product residue, follow label warnings even after container is emptied. 14 TRANSPORT INFORMATION DOT UN No.: UN1648 Proper Shipping Name: Acetonitrile Class: 3 Packing Group: II Label(s): 3 TDG UN No.: UN1648 Proper Shipping Name: ACETONITRILE Class: 3 Packing Group: II IATA UN No.: UN1648 Proper Shipping Name: Acetonitrile Class: 3 Packing Group: II Label(s): Flamm. liquid IMDG UN No.: UN1648 Proper Shipping Name: ACETONITRILE Class: 3 Packing Group: II EmS No.: F-E, S-D 15 REGULATORY INFORMATION Canadian Controlled Products Regulations: This product has been classified according to the hazard criteria of the Canadian Controlled Products Regulations, Section 33, and the MSDS contains all required information. WHMIS Classification: B2, D1A, D2B Mexican Dangerous Statement: This product is dangerous according to Mexican regulations. Inventory Status: US TSCA inventory Status: All the components of this substance are listed on or are exempt from the inventory. For purposes of 40 CFR , this product is for Research and Development (R&D) Use Only. NA ENGLISH MSDS 6 / 8

7 US Regulations CERCLA Hazardous Substance List (40 CFR 302.4): Chemical Name Acetonitrile RQ 5000 lbs SARA Title III Section 302 Extremely Hazardous Substances (40 CFR 355, Appendix A): Not regulated. Section 311/312 (40 CFR 370): X Acute (Immediate) X Chronic (Delayed) X Fire Reactive Pressure Generating Section 313 Toxic Release Inventory (40 CFR 372): Chemical Name CAS-No. Concentration Acetonitrile > 60% Clean Air Act (CAA) Section 112(r) Accidental Release Prevention (40 CFR ): Not regulated. Clean Water Act Section 311 Hazardous Substances (40 CFR 117.3): Not regulated. Drug Enforcement Act: Not regulated. TSCA: TSCA Section 4(a) Final Test Rules & Testing Consent Orders: Acetonitrile TSCA Section 5(a)(2) Final Significant New Use Rules (SNURs) (40CFR 721, Subpt. E): Not regulated. TSCA Section 5(e) PMN-Substance Consent Orders: Not regulated. TSCA Section 12(b) Export Notification (40 CFR 707, Subpt. D): Acetonitrile State Regulations California Safe Drinking Water and Toxic Enforcement Act of 1986 (Proposition 65): Not regulated. Massachusetts Right-To-Know List: Acetonitrile Michigan Critical Materials List (Michigan Natural Resources and Environmental Protection Act (Act. 451 of 1994)): Not regulated. Minnesota Hazardous Substances List: Acetonitrile New Jersey Right-To-Know List: Acetonitrile Pennsylvania Right-To-Know List: Acetonitrile Rhode Island Right-To-Know List: Acetonitrile NA ENGLISH MSDS 7 / 8

8 16 OTHER INFORMATION HAZARD RATINGS Health Hazard Fire Hazard Instability Special Hazard NFPA Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe NFPA Label colored diamond code: Blue - Health; Red - Flammability; Yellow - Instability; White - Special Hazards Health Hazard Flammability Physical Hazard Personal Protection HMIS 2* 3 0 H Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe *- Chronic Health Effect Personal Protection codes: H - Goggles, Gloves, Apron, Vapor Respirator HMIS Label colored bar code: Blue - Health; Red - Flammability; Orange - Physical Hazards; White - Special Disclaimer: This MSDS has been prepared using information from sources considered technically reliable. It should not be relied upon as a product specification. The company makes no warranty of any kind, express or implied, concerning the safe use of this material in your process or in combination with other chemical substances. Every user of the product is responsible for evaluating its own processes and conditions of use and selecting appropriate protective measures for employee exposure. NA ENGLISH MSDS 8 / 8

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