Preservation effects of water-soluble chitosan on surimi product

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1 South China Fisheries Science Vol. 7, No. 4 Aug., 2011 doi: / j. issn ,,, (, ) : ( TVB-N) ( TBA) ph, 4,, TVB-N TBA, 2. 0%,, 9 d d : ; ; : S : A : ( 2011) Preservation effects of water-soluble chitosan on surimi product SHI Haifeng, GAO Jian, YING Jie, LIN Jianyu, XU Xinbo, LUO Hongyu ( School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan , China) Abstract: We studied the preservation effects of water-soluble chitosan at different concentrations on cold storage of surimi product. The sensory score, total bacterial count, total volatile base nitrogen ( TVB-N), thiobarbituric acid ( TBA) and ph were observed to e- valuate the changes in quality of surimi product stored at 4. The results indicate that water-soluble chitosan effectively inhibits the bacterial growth and propagation, prevents the decline of sensory quality, as well as postpones the increase of TVB-N and TBA. 2. 0% water-soluble chitosan achieves the best preservation effect, prolonging the shelf life from 9 d to d as compared with the control. Key words: water-soluble chitosan; surimi product; cold storage,, ;, - 18,,, ( Chitin) - -D- -1 4,,,,, [ 1-5] [ 6-8],, : ; : : ( ) ( 2009R ) : ( ), shflovenono@ 126. com :, lisa8919@ 163. com

2 50 7, ( Penacus orientalis) ( Carassius auratus) ( Gadus Macrocephalus) [ 9-20 ] ( Clupea pallasi),,, 5 min (, ) 20, 4 min min 3 min [ 1 2], 1. 2 ( ), 95% ( ) ; ( 1. 0% 1. 5% 2 ) ; 4, A ( ), B 1. 0%, C 1. 5% 1. 3 LDZX-40BZ 1. 6 ( ) ; HWS NYT ( ) ; NR , 7 ( ) ; DL-5, 4 ( ) ; 5, HH-4 ( 1 ) ; ) ; TGL-16C ( GB/ T UV-1100 ( ) ; FE20K ( Mettler Toledo) 1. 4, 10. 0%, 2. 0%, 1. 5,,, 2. 0%,, D 2. 0% ( TVB-N) SCT , 1 Tab. 1 Sensory evaluation standards of fish-ball level item fresh 9 10 relatively fresh 7 8 general 5 6 spoilage 3 4 serious spoilage 1 2 appearance meat quality odor,,,,

3 4 : 51 TVB-NpH g, 100 ml,, 30 min, ph ( TBA), , SPSS 17. 0, t, T,, Origin 8. 0 P < ,, B C D A ( P < 0. 05) ( 1) 3 d ( P > 0. 05),,, ; 4 ( P <0. 05), A, ; 9 A, ; 7 B C D ( P > 0. 05),,, ; 11 B ; 13 C D,, C 12, 13 D 2. 0% cfug - 1,, 2. 2, 2 [ 20],, A,,, 3 ( P <0. 05), 9 TVB-N, TVB-N cfu g - 1, NY / T ( ) d) ( P >0. 05), cfu g - 1 B C D 3 7 d,, 10 4 cfu g - 1,,, ( P < 0. 05) 11 B TVB-N ( 3) 3 A Fig. 2 1 Fig. 1 Sensory evaluation of fish-ball 2 Change of total bacterial count in fish-ball during storage 2. 3 TVB-N TVB-N ( 0 5,

4 52 7 Fig. 3 3 TVB-N Change of TVB-N in fish-ball during storage Fig. 4 4 ph Change of ph of fish-ball during storage TVB-N ( P < 0. 05), ; 9 A TVB-N mg g - 1, mgg - 1, mg g - 1,, B TVB-N C D TVB-N GB , C D 13 D ( mg g - 1 ) 2. 4 ph,, ph ( P > 0. 05), ph 6. 6 ( 4) 5 d ph ( P <0. 05),, Fig. 5 Change of TBA in fish-ball during storage ph 9 A ph 7. 2, B 6. 9, C D ph,, ( O 2 ) ( CO 2 ), D ph ( P < 0. 05) 2. 5 TBA TBA MDA, SPSS TBA, TVB-NTBA ph nm, 1, TBA %,, TBA F ( P < 0. 05), A TBA, = TVB-N mgg - 1 D TBA, mgg - 1 TBA ,, D > C 5 TBA, , ph TBA A , B , C , D

5 4 : 53 > B > A 3,, ( TVB-N) ( TBA) ph TBATVB-N ph,, 2. 0% [ 4],,, 9 d d,,, [ J]., [ 9 ] ( Penaeus vannamei), TVB-N [ 12],,,, [ 8],,,,, , : 2004( 7 ) : ZHU Bin. Application of chitosan and its derivatives in the field of food industry[ J]. Chin Western Food, 2004 ( 7 ) : ( in Chinese) [ 2],,. [ J]., 2006( 4) : ZHANG Qinggang, YU Shanming, YAO Xu, et al. Study on an anti-staling coating made of chitosan [ J]. 2006( 4) : ( in Chinese) Sci Technol Food Ind, [ 3],. [ J]., 2001, 10( 4) : WANG Hong, SHEN Yuexin. The antibiotic activities of chitosan with different deacetyl degrees [ J]. J Shanghai Fish Univ, 2001, 10( 4 ) : ( in Chinese), 2002( 3) : [ J]. YANG Yuan e, LUO Faxing, TAN Wei. A new crude food preservative-chitosan[ J]. J Wuhan Polytechnic Univ, 2002 ( 3 ) : ( in Chinese) [ 5],., 2005, 26( 8) : LIU Zhenghao, WU Guangchen, WANG Tingxin. Mechanism and the application of chitosan preserving food[ J]. Food Sci, 2005, 26( 8 ) : ( in Chinese) [ 6],,. [ J]., 2004, 6( 1) : ZHAO Yuqing, ZHENG Zhaoyan, WANG Bing, et al. The preparation of CTS-X and the preservation of lychee[ J]. J Dalian Nationalities Univ, 2004, 6( 1) : ( in Chinese) [ 7]. [ J]., 2003, 3 ( 1) : GAO Haisheng, LI Chunhua, CAI Jinxing, et al. Advances on the study of natural fresh-keeping agents for fruits and vegetables[ J]. Chin Inst Food Sci Technol, 2003, 3( 1 ) : ( in Chinese). [ J]., 2009, 37( 7 ) : YUAN Zhi, WANG Mingli. Application of chitosan in storage and freshness preservation of fruits and vegetables[ J]. Sci, 2009, 37( 7) : ( in Chinese) J Guizhou Agric [ 9],. [ J]., 2008, 29 ( 6 ) : XU Limin, XUE Changhu, LI Zhaojie, et al. Influence of watersoluble chitosan on the changes of quality and spoil age microflora of Penaeus vannamei[ J]. Sci Technol Food Ind, 2008, 29( 6) : ( in Chinese) [ 10],. [ J]., 2010, 38( 2) : [ 1]. [ J]. YANG Shengping, XIE Jing, TONG Yi. Effects of different concentrations chitosan to fresh: keeping of trichiutus haumela in cold

6 54 7 storage[ J]. J Shanxi Agric Sci, 2010, 38 ( 2) : ( in Chinese) [ 11],,. [ J]., 2009, 37( 6 ) : [ J]., 2010, 26( 4) : YANG Shengping, XIE Jing, TONG Yi, et al. Effects of chitosan combined with different concentrations of tea polyphenols on preservation of Trichiutus haumela [ J]. Jiangsu J Agric, 2010, 26 ( 4) : ( in Chinese) 2008, 28( 7 ) : WANG Xiujuan, ZHANG Kunsheng, REN Yunxia. Study on preservation of shrimp by coating with chitosan[ J]. Food Sci, 2008, 28( 7) : ( in Chinese) [ 13],,. [ J]., 2005 ( 10 ) : [ J ]., 2009, 21( 11 ) : WANG Jungang, LU Shiling, LI Kaixiong, et al. Study on preservation effect of composite natural preservatives to chilled mutton [ J]. Acta Agriculturae Jiangxi, 2009, 21 ( 11 ) : ( in Chinese) [ 14],. [ J]. 2005, 26 ( 4) : LIU Kun, GAO Hua. Study on new technology of coating preservation of fish[ J]. Mar Fish Res, 2005, 26( 4 ) : ( in Chinese) [ J]., 2010, 26( 4 ) : ZHAO Haipeng, LI Qingchun, XIE Yaojing, et al. Effects of compound biological preservative on prawn [ J]. Jiangsu J Agric, 2010, 26 ( 4) : ( in Chinese) [ 16]. LAN Weiqing, XIE Jing. Study progress on the effect of aquatic products preservation with several bio-preservatives[ J]. Agric Sci, 2009, 37 ( 6) : ( in Chinese) J Shanxi [ 17],. [ J]., 2010, 26( 7) : [ 12],. [ J]. LIU Yongji, LI Jianrong, ZHU Junli, et al. Effects of modified atmosphere packaging on qualities of surimi product during refrigerated storage[ J]. Trans Chin Soc Agric Eng, 2010, 26( 7) : ( in Chinese) [ 18],. LI Hongxia, HUANG Yanchun, XIONG Shanbo, et al. Studies on quality assessment factors for surimi-based products during storage [ J]. Sci Technol Food Ind, 2005 ( 10 ) : ( in Chinese) [ 19]. [ J]., 2002( 4 ) : WANG Xuerong, ZHOU Weilu. Study on water holing effects of mixed phosphates on surimi product[ J]. 2002( 4) : ( in Chinese) Sci Technol Food Ind, [ 20],. [ J]. 2006, 31( 6) : [ 15],,. JING Yingjun, HAO Youjin, QU Yaohui, et al. Preliminary studies on antibacterial mechanism and analysis of antibacterial activity of chitosans [ J]. Chin J Antibiot, 2006, 31 ( 6 ) : ( in Chinese)

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