1 DAVIDS BIOTECHNOLOGIE GMBH CATALOG 2016 Custom Antibodies 2 Davids Biotechnologie GmbH Custom Antibodies: Introduction How to order Custom Antibodies Antigen Production 4 Suitable Antigens Peptides As Antigen Proteins From SDS-Gel Pieces Polyclonal Antibodies 8 Rabbit Antisera Chicken Egg Yolk Antibodies Small Mammalians Phosphospecific Antibodies Monoclonal Antibodies 14 Development in vitro Monoclonal Antibodies Production And Purification Antibody Purification 17 Affinity Purification ProteinA Purification Price List and Imprint 19 Price List Imprint Picture Index
2 2 Custom Antibodies CUSTOM ANTIBODIES Davids Biotechnologie Davids Biotechnologie was founded 1996 in Regensburg, a world cultural heritage city in Germany. We offer all kind of custom made antibodies. This includes Polyclonal antibodies from rabbits, chicken, mice, rats or guinea pigs. Additionally we offer monoclonal antibodies from mice or rats. Any kind of customer provided or Davids made antigens are possible. We developed animal and ecologically friendly protocols to produce the antibodies. This leads to efficient methods and low prices. Furthermore Davids developed methods to produce antibodies within 28 days (SuperFast) or with a very low amount of antigen (LowDose). Our purification methods are very well established and we offer Affinity, ProteinA, Depletion, Size-Exclusion, Ion-Exchange, IgM or any other purifications that fits to your antibody project. We offer different kind of antigen production services. This includes peptide synthesis, protein expression, SDS-gel extractions and more to generate your antigens for the antibody production. Additionally we modify your antibodies like fragmentation, enzyme or dye conjugations. Custom Antibodies: Introduction We produce antibodies from antigens provided by the customer. Our customers are able to send us their antigen (protein, peptide sequence, small molecules, DNA, cells) and we perform the immunization of the desired animal. After the immunization process the customer receives the antisera or the purified antisera for their research. For monoclonal antibodies we isolate spleen cells, perform the fusion with myeloma cells and screen for positive clones. You receive the antibody producing clone and/or purified antibodies from your antibody producing clone. How to order Custom Antibodies We keep the order process simple. Just send us your antigen with the catalog number or the desired final antibody and we start with the antibody production as soon as your antigen arrives. For further questions please contact us.
3 Custom Antibodies 3 Our Antibody Packages Includes everything you need for your final antibody. Cat.No. Animal Preimmune Test Bleed Final Product Purification Polyclonal Rabbit Antibodies A053 One Rabbit 0.5 ml 0.5 ml ml Antiserum A054 Two Rabbits 2 x 0.5 ml 2 x 0.5 ml ml Antiserum A055 One Rabbit 0.5 ml 0.5 ml ml Antiserum A056 One Rabbit 0.5 ml 0.5 ml ml Antiserum A061 Two Rabbits 2 x 0.5 ml 2 x 0.5 ml Phosphospecific Antibodies additional service available additional service available additional service available additional service available Affinity Purified & Depleted Polyclonal Chicken Egg Yolk Antibodies A001 One Chicken 0.5 ml - PrepI from 8 eggs A002 Two Chicken 2 x 0.5 ml - PrepI from 16 eggs A003 One Chicken 0.5 ml - PrepII from 8 eggs A063 Two Chicken 2 x 0.5 ml - Phosphospecific Antibodies PrepI PrepI PrepII Affinity Purified & Depleted A201 One Guinea pig 0.1 ml ml A205 One rat 0.1 ml ml A402 Two mice 2 x 0.05 ml ml Polyclonal Antibodies from small mammalians additional service available additional service available additional service available You can order additional services to our basic antibody packages. Simply add an antigen preparation/synthesis service or a purification method to your antibody package.
4 4 Antigen Production ANTIGEN PRODUCTION Suitable Antigens for the Antibody Production For the antibody production nearly any kind of antigen can be used. Usually proteins, peptides, SDS-Gel pieces, DNA, cells or small molecules are used for polyclonal or monoclonal antibodies. When you have purified protein available the native protein can be used as antigen. When the purification of the protein is difficult or when the purified protein shows multiple bands in a SDS-gel you can use our SDS-Gel Extraction service. For proteins you only know the sequence from you can use our Peptide Antibody Service. Here we are looking for peptides that represent your protein. Our prediction program predicts the antigenicity, solubility and epitopes of the peptides. If you have any questions regarding your antigens you can just send us an . Customer provided proteins as antigen We start with the immunization as soon as your antigen arrives. Proteins are usually shipped with dry ice, cool packs or lyophilized at room temperature, depending at what conditions the protein is stable. The buffer should be non-toxic and at a physiological ph. The buffer can contain additions like glycerol, urea, etc (see Figure 1). We recommend a protein concentration between 0.2 and 2 mg/ml. Figure 1: The buffer can contain the following ingredients. Examples for buffer contents. Buffer Conditions Maximum Concentration Buffer Conditions Urea 2 M Glycerol 10% NaCl 200 mm SDS 0.1% Imidazole 200 mm Triton X % Dithiothreitol (DTT) 2 mm EDTA 1 mm Maximum Concentration Citrat or Acetat 100 mm TRIS 100 mm Other components on demand. We also offer buffer exchange services. Proteins from SDS-Gel Pieces: Cat.No. A705 We are able to use your protein specific SDS-PAGE gel bands as antigen. The advantage is that you save time with complicated purification steps. In addition you are able to get rid of even small protein contaminations. The final antibodies are usually very good for applications like western blot. The gel extraction is simple. Prepare a solution with at least 0.5 mg protein for the immunization of one rabbit for example and additional 0.5 mg protein if you want to order an affinity purification. Just run multiple bands with your protein solution, dye
5 Antigen Production 5 the gel with coomassie and cut all bands that belong to your protein from the SDS- PAGE gel (see Figure 2). Send the gel pieces in a 2-ml or 15-ml tube at room temperature without the addition of any solvents. Figure 2: Scheme of the gel extraction Peptide Antibodies: Cat.No. P101 and P102 If you only know the sequence of your protein or when your protein is hard to express and purify we can synthesize peptides that represent your whole protein. We help you to find peptides that represent your protein (see Figure 3) - this service is free. These peptides have to be antigenic, soluble and must have good epitopes. For a higher chance to use the best peptide, you can immunize one animal with two different peptides. This doubles your chance to use the best peptide for your applications. The peptide production is done with solid phase synthesis. Quality controls are done by HPLC and MS with each peptide. The purity is about 80% (70 98%) for the immunizations. The synthesized peptides are conjugated to a carrier molecule like KLH to improve the antigen presentation of the small peptides. The synthesis, conjugation and/or the immunizations can be performed by Davids. To receive monospecific antibodies we recommend performing affinity purification.
6 6 Antigen Production Figure 3: Peptide Antibodies We can produce peptide antibodies from rabbits, chicken, mice, rats and guinea pigs. Just send us your protein sequence (amino-acid one letter code preferred) and we analyze the sequence for free and handle your data confidentially. An order is only placed when you confirm one or more peptides. Cells as antigen: Cat.No. K105 We recommend using our Cell Immunization KIT for optimal presentation of the cell surface antigens. You can send us your dead cells and we perform the preparation of the cells in our lab. Small Molecules as antigen: Cat.No. P105 We are able to use your small molecules as antigen. To enhance the antigenicity and the epitope presentation of small molecules we use different conjugation methods to bind the antigen to a carrier like KLH, Ovalbumin, Tyroglobulin, BSA or Dextran. DNA as a small molecule is handled differently. We prepare the DNA for the immunization with multiple steps. For further information you can write us an .
7 Antigen Production 7 Overview of suitable antigens We offer the antibody production with the following antigens. Some antigens need additional synthesis or preparations that have to be ordered in addition to your antibody package. The needed amount is calculated for the immunization of one rabbit. For smaller animals like mice, guinea pigs or rats less antigen is sufficient. If you do not fit the requirements just write us an . We already successfully produced antibodies with less amount of antigen. Catalog Number Description We Need for one animal Proteins Antigen Provided by Customer Your Protein ( mg) Peptides P101 One Peptide Your Protein Sequence (amino acids) P102 Two Peptides Your Protein Sequence (amino acids) SDS-Gel Pieces A705 Gel Extraction Your SDS-Gel Pieces ( mg) Cells K105 Cell Preparation Dead Cells ( cfu) DNA A703 DNA Preparation Your DNA (1 2 mg) Small Molecules P105 Conjugation Your small molecule (0.5 5 mg)
8 8 Polyclonal Antibodies POLYCLONAL ANTIBODIES Introduction Polyclonal antibodies are the antibodies of choice when you need inexpensive and fast produced high affinity antibodies. The polyclonal antibodies recognize multiple epitopes of the antigen. With polyclonal peptide antibodies we are able to produce antibodies that target an epitope region which can be helpful when you want to distinguish between protein families or if you want to detect multiple families with one antibody batch. Polyclonal Rabbit Antisera We use New Zealand White Rabbits for the production of your polyclonal antibodies. It is the most used species to produce custom polyclonal antibodies. You receive a good amount of high-titer and high-affinity antibodies at low costs with New Zealand White Rabbits. In addition rabbits are easy to handle and additional test sera are easy to prepare. The final amount of antibodies is very good comparing with mice, rats or guinea pigs. We have different protocols for the needs of our customers. Our standard 63-day protocol fits to most applications. For customers who need their antibodies faster we offer our SuperFast procedure, which takes 28-days. Here we use a superior adjuvant and a proprietary immunization schedule. The titers are comparable to 63- or 90-day protocols (Figure 4). We are able to immunize one (A053), two (A054) or more rabbits with the same antigen. Figure 4: Comparison of Titer Development: 28-day/63-day Protocol 28-day 63-day day For expensive antigens or antigens from where you only have a small amount available we offer our LowDose Protocol (Cat.No. A056). Here we are able to produce antibodies with a smaller amount of antigen < 0.2 mg.
9 Polyclonal Antibodies 9 Cat.A day Protocol Cat.A day Protocol Cat.A day Protocol Schedule 28 days 63 days 93 days Immunizations Serum Amount ml ml ml Suitable Antigens Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Antigen Needed mg Protein mg Protein mg Protein Antigen Concentration mg/ml mg/ml mg/ml Antigen Buffer Non Toxic buffers Non Toxic buffers Non Toxic buffers Additional Purification Available Precipitation (R001) Protein A (R008) Affinity Purification (R007) Precipitation (R001) Protein A (R008) Affinity Purification (R007) Precipitation (R001) Protein A (R008) Affinity Purification (R007) Our rabbit antisera custom antibodies include preimmune serum from day 0, a test bleed at day 35 (not available with our 28-day protocol), an ELISA titer determination and the final bleed. You can order additional purifications for your rabbit antisera. Polyclonal Antibodies from Chicken Egg Yolk Our polyclonal chicken egg yolk antibody services include the immunization, egg collection and the egg yolk preparation of up to 8 immune eggs. This PrepI preparation of the immune eggs is ready to use and is comparable with raw sera from mammalians. Additional preparations like our PrepII uses chromatographic polishing steps to remove most lipids and lipoproteins of the egg yolk preparation. This PrepII is comparable with ProteinA purifications and is superior in most applications. Additionally we are able to perform affinity purifications with the egg yolk. For antibodies that are used in cell cultures we offer our PrepIII multi-step purification. This fraction can be used directly in cell cultures. It is chromatographically purified, sterile filtered and has low endotoxin and low DNA.
10 10 Polyclonal Antibodies Cat.A day PrepI Cat.A day PrepI Cat.A day PrepII Schedule 43 days 43 days 43 days Immunizations Animals Eggs Included 8 eggs correspond to 100 ml serum 2 x 8 eggs correspond to 200 ml serum 8 eggs correspond to 100 ml serum Suitable Antigens Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Antigen Needed mg Protein mg Protein mg Protein Antigen Concentration mg/ml mg/ml mg/ml Antigen Buffer Non Toxic buffers Non Toxic buffers Non Toxic buffers Included Purification PrepI PrepI PrepII Additional Purification Available PrepII (A003) Affinity Purification (R007) PrepII (A003) Affinity Purification (R007) Affinity Purification (R007) Our chicken egg yolk antibodies includes a ready to use egg yolk preparation PrepI or PrepII and preimmune egg yolk preparation. The advantage of chicken egg yolk antibodies is that you are able to receive additional antibodies from the same animal. The chicken keeps on laying immune eggs. If you decide within 30 days after you receive the first antibody preparation that you need more antibodies we can prepare them from the same animal (A012). You can order additional purifications for monospecific antibodies.
11 Polyclonal Antibodies 11 Advantages of Chicken Egg Yolk Antibodies One hen produces egg yolk antibodies that are comparable to the amount of 4000 ml serum (8000 ml of blood) in one year. Hens need less amount of antigen than rabbits. The antibody output is up to 10 times higher compared with rabbits. This saves costs and reduces animal treatments. Eggs from chicken egg yolk (also called IgY) are used in the same way as mammalian IgG from serum. (RIA, ELISA, WesternBlots, Immunohistochemistry, Ouchterlony, Nephelometry, Latex agglutination, Immunoelectrophoresis, Enzyme inhibition assay, Immunosorbent assays, FACS, etc.). Secondary antibodies with all usual conjugates are commercially available. IgG from chicken egg yolk can be fragmented like mammalian IgG utilising Papain or Pepsin to produce Fab or Fab fragments. IgG from chicken egg yolk shows low non-specific binding because it does not bind to complement factors, rheumatoid factors, mammalian IgG, Staphylococcus aureus ProteinA or Streptococcus ProteinG. Chicken egg yolk antibodies are a good possibility to get high specific and high binding antibodies directed to mammalian antigens. Sometimes they are the only possibility. The large advantage is the phylogenetic distance between birds and mammals. In consequence you need less amount of antigen and fewer animals which lead to reduced costs to get an optimal antibody for your purposes. The production of egg yolk antibodies is a method to avoid and to reduce unnecessary maltreatment of animals (no bleedings, fewer immunisations, species adapted keeping). IgG from egg yolk is stable at 4 o C for a long time and could be frozen directly or after the addition of 25 50% glycerol (end concentration) without loss of activity
12 12 Polyclonal Antibodies Polyclonal Antibodies from small mammalians: Mouse, Rat, Guinea pig We offer polyclonal antibodies in mice, rats or guinea pigs. The service includes the immunization, preimmune taken before the first immunization and the final bleed. Additionally an ELISA titer determination is performed. Cat.A day: 2 Mice Cat.A day: 1 rat Cat.A day: 1 guinea pig Schedule 63 days 63 days 63 days Immunizations Animals Serum 2 x ml 2 10 ml 5 15 ml Suitable Antigens Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Protein Protein (SDS Gel) Peptide DNA Cells Small Molecules Antigen Needed mg mg mg Antigen Concentration mg/ml mg/ml mg/ml Antigen Buffer Non Toxic buffers Non Toxic buffers Non Toxic buffers Phosphospecific Polyclonal Antibodies For phosphospecific antibodies we synthesize the non-phospho-peptide and the phosphopeptide. The customer receives an aliquot of the synthesized peptides and the affinity purified and depleted antibodies. Cat.A061 Cat.A063 Schedule 63 days 63 days Immunizations 5 5 Animals 2 rabbits 2 hen Peptide (phospho) 5 mg for customer 5 mg for customer Peptide (standard) 5 mg for customer 5 mg for customer
13 Polyclonal Antibodies 13 For antibodies that recognize a phosphorylated site of a protein. After synthesis and conjugation of the phosphospecific peptide (1) the animals are immunized (3) with the conjugated phospho-peptide (2) to produce antibodies. After antibody production the serum is affinity purified with the phospho-peptide to get specific antibodies (4). In this stage of the process there are still some antibodies recognizing the nonphosphopeptide. To get rid of these unwanted antibodies a depletion is necessary. The affinity purifies serum is depleted with a non-phosphopeptide depletion matrix. The result are phosphospecific antibodies (5). Figure 5: Scheme of phosphospecific antibody generation.
14 14 Monoclonal Antibodies MONOCLONAL ANTIBODY DEVELOPMENT Introduction When you need antibodies that recognize one single epitope and antibodies that are reproduced constantly, monoclonal antibodies are the perfect choice for you. In addition every batch of your produced antibodies has no or very low variability. Monoclonals are very specific and thus usually have less background in many applications. In combination with their reproducibility, monoclonals are very good for different assays. We develop your custom monoclonal antibodies from mice or rats with any kind of antigen. To receive the best results we alter the concentration of the antigen for the immunizations. This ensures optimal immune response and we continue with the best mouse. In addition you can start or stop with each step. This enables you to send us spleen cells and we just perform the fusion and screening. Or we perform the immunization and you continue with the fusion and screening in your own lab. The development of monoclonal antibodies is very specific for each customer. We can adapt our protocols for your special needs. This includes immunization protocols, screening conditions or alternative screening substrates. Step 1: Immunizations: Cat.M001 The graph shows the immune response of 4 mice immunized with different antigen concentrations. The mouse with the best titer is usually used for the next steps. Immunization of 4 BALB/c mice or rats with different antigen concentrations to have a broader spectrum of responses ELISA titer determination Testserum for the customer to perform their own ELISA to determine the best titer More boosts or start of the fusion Step 2: Spleen Cell Isolation: Cat.M002 The chosen mouse from Step 1 gets a final immunization to boost the antibody producing cells Spleen cells are isolated for direct fusion Part of the cells is cryoconserved as backup The spleen cells can be send to the customer for self made fusion or RNA isolation
15 Monoclonal Antibodies 15 Step 3: Fusion: Cat.M003 Spleen cells from Step 2 Fusion with immortalized myeloma cell line Step 4: Screening for positive clones: Cat.M004 Growing of the fusion cells Screening of the clones in cell culture plates Up scaling of stable and positive clones and further screening Isolation and storage of the best clones You receive 10-ml supernatant of the best clones for your own applications You receive the best clones in frozen vials or in cell culture Step 5: Subcloning: Cat.M005 Subcloning of the best positive clones with our limiting dilution method Ensure that the clone is truly monoclonal Improve long-term stability IN VITRO MONOCLONAL ANTIBODIES In vitro antibodies are save time and money as you spare the immunization procedure of the animals. The advantage is that you can use antigens like selfantigens or toxic antigens. Our in vitro monoclonal antibody service includes the antigen preparation. The antigen is incubated with spleen cells from mice after the preparation. The incubated cells are used for the fusion with myeloma cells. The screening for positive clones is included as well. You receive supernatants of the positive clones and the clone itself for your own antibody production. Just order our all-inclusive in vitro antibody package Cat.No.M010.
16 16 Monoclonal Antibodies MONOCLONAL ANTIBODY PRODUCTION We can produce your monoclonal antibodies from your clone. Just send us your clone and we produce and purify your antibody. We have different purification methods available to adapt the purification to your antibodies. Cat.M Cat.M Cat.M301-XXX Production Amount 20 mg 100 mg Individual
17 Antibody Purification 17 ANTIBODY PURIFICATION Affinity Purification: Cat.R007 Affinity Purification is a chromatography, which is used to separate molecules in a solution. The molecule of interest (i. e. antibodies) makes a specific interaction to the affinity matrix (i. e. antigen covalently bound to the affinity matrix). All non-specific molecules (i. e. antibodies that do not bind the antigen) cannot bind the affinity matrix and are washed. The molecule of interest is eluted afterwards. Affinity purified antibodies have large advantages in many applications. Usually you can use higher concentrated antibodies in your applications like IHC, WesternBlot or DotBlots with reduced background. The first step of the affinity purification is the creation of the affinity matrix. For each antibody a specific matrix is produced. The matrix material is incubated with the antigen under special conditions. Under these conditions covalent bindings are formed between the matrix and the antigen. In the next step the matrix is used to purify the antisera or the PrepI from chicken egg yolk. The specific antibodies (red in Figure 6) bind the antigen (orange in Figure 6) covalently bound to the matrix. The unspecific antibodies (blue in Figure 6) are washed and the specific antibodies can be eluted afterwards. Figure 6: Scheme of an affinity purification Our affinity purification includes the creation of the affinity matrix and the affinity purification to receive antigen-specific antibodies. Cat.R007 is for one serum, Cat.R007-2 is for two sera from the same antigen. We can use proteins, peptides, small molecules, nucleic acids and cells for covalent conjugation to the affinity matrix.
18 18 Antibody Purification ProteinA Purification: Cat.R008 ProteinA and ProteinG are proteins from the cell wall of S. aureus. ProteinA binds the Fc part of the antibodies, especially from IgG. With the ProteinA or G purification all IgG's are purified from the antiserum. Most of the lipids, polysaccharides, IgE or IgM are removed from the fraction. Our ProteinA purification Cat.R008 includes serum preparation and the purification. Depletion: Cat.R101 With antibody depletion you can remove antibodies from a fraction you do not want. For example, you can remove antibodies that recognize a HIS/MBP/Strep-tag or antibodies that recognize E. coli proteins (Figure 7). The tag is bound to an affinity matrix and the anti-tag-antibodies bind to the affinity matrix. All unbound antibodies are used for further applications. Figure 7: Scheme of a HIS-tag depletion.
19 Price List and Imprint 19 PRICE LIST Antigen Preparation Catalogue Number Description Price in EUR Peptides for Antibodies P101 One Peptide Synthesis up to 20 aa, >70% purity 344,00 P102 Two Peptides Synthesis, up to 20 aa, >70% purity 473,00 Antigen Preparation Service A703 SDS-Gel Extraction 43,00 K105 Cell Immunization Preparation 89,00 P105 Conjugation to a carrier like KLH, BSA, Ovalbumin 68,00 Polyclonal Antibodies Catalogue Number Description Price in EUR Polyclonal Rabbit Antibodies A053 One rabbit antiserum (40 90 ml) 274,00 A054 Two rabbit antisera, same antigen ( ml) 516,00 A055 SuperFast antiserum, 28-days (40 90 ml) 344,00 A056 LowDose antiserum, 63-days (40 90 ml) 368,00 A061 Phosphospecific Antibodies, two rabbits, 63-days 1595,00 Polyclonal Chicken Egg Yolk Antibodies A001 One hen complete package, PrepI 368,00 A002 Two hen complete package, PrepI 602,00 A003 One hen complete package, PepII 395,00 A012 Additional Immunization, 8 egg collection and PrepI 150,00 A063 Phosphospecific Antibodies, two chicken, 63-days 1595,00 Polyclonal Antibodies from small mammalians A201 One guinea pig, 63-days 260,00 A205 One rat, 63-days 195,00 A402 Two mice, 63-days 144,00 Purifications Catalogue Number Description Price in EUR Purification R001 Fractionated ammonium sulphate precipitation 89,00 R007 Affinity Purification 233,00
20 20 Price List and Imprint R007-2 Affinity Purification from two sera, same antigen 368,00 R008 ProteinA Purification 189,00 Depletion R101 Anti-tag Depletion 129,00 Monoclonal Antibodies Catalogue Number Description Price in EUR Monoclonal Antibody Development M001 Immunization of 4 mice 795,00 M002 Isolation of spleen cells from one spleen 172,00 M003 Fusion of the spleen cells with myeloma cells 430,00 M004 Screening and isolation of positive clones 1591,00 M005 Subcloning of one clone 610,00 Additional Monoclonal Antibody Services M006 Subclass Determination: IgG/IgM 21,00 M010 In vitro Monoclonal Antibody Service 3595,00 Monoclonal Antibody Production M Monoclonal Antibody Production & Purification : 20 mg 795,00 M Monoclonal Antibody Production & Purification : 100 mg 1495,00 Cell Services Catalogue Number Description Price in EUR Isolation of Cells N101 Spleen Cell Isolation from one immunized animal 172,00 N201 PBMC: Isolation of lymphocytes from whole blood 43,00 O101 Bone Marrow (femur) lymphocytes 250,00 T901 RNA Isolation from cells: 10 6 to ,00
21 Price List and Imprint 21 IMPRINT Imprint Davids Biotechnologie GmbH Röntgenstraße Regensburg Deutschland Vertretungsberechtigter Geschäftsführer: Dr. Michael Werner Davids Tel.++49 (0) Fax.++49 (0) Register und Registernummer: Registergericht Regensburg HRB9834 Umsatzsteuer-Identifikationsnummer gem. 27a UStG: DE Inhaltlich Verantwortlicher nach 55 II RStV: Dr. Michael Werner Davids, Röntgenstraße 3, Regensburg, Deutschland Design and Editorial: Davids Biotechnologie GmbH Tel.: 0941/ Pricing All prices are given in EURO without VAT and are subject to change without notice. For shipping and handling including packing material we charge EURO 26. The terms of payment are strictly 30 days from date of invoice after delivery directly to. Picture Index Picture Picture Name Author / Quelle Cute Rabbit camellias / Fotolia.com Mouse on the edge aaali / Fotolia.com Conditions of sale The products in this catalog are for laboratory research only, unless otherwise specified.
22 22 Price List and Imprint Davids Biotechnologie cannot be held responsible for the usage of antibodies. We cannot guarantee that the antibodies directed against antigens selected by the customer are useful in the customers (special) assays. But we are happy to make supportive suggestions that however do not correspond with a guaranty. We wait for 21 days after the delivery of the egg yolk antibodies. Unless the customer informs us about the continuation of the production, we stop the production irreversibly. This catalogue remains valid until the publication of a new one including new prices and new conditions. Shipping conditions Antibodies are stable for several weeks from 4 C to 38 C. Within this range of temperature the antibodies are shipped. Freezing and thawing cycles are prevented in winter by addition of cooling bricks at 10 C starting temperature. Confidentiality It is our philosophy that Davids Biotechnologie guarantees that all customers related data are handled absolutely confidential.
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TECHNICAL NOTE The Use of Antibodies in Immunoassays Introduction Structure of an IgG Antibody Immunological reagents are the backbone of every immunoassay system. Immunoassays can be utilized to quantitatively
Chapter 6: Antigen-Antibody Interactions I. Strength of Ag-Ab interactions A. Antibody Affinity - strength of total noncovalent interactions between single Ag-binding site on an Ab and a single epitope
DELFIA DELFIA assays bring convenience in monoclonal development INTRODUCTION The DELFIA system provides a method of discovering specific, high-affinity monoclonal antibodies (Mabs) even in low concentrations
Thomas Boenisch, MS, and Jim Hudson, PhD Immunoglobulins The vertebrate immune system has the amazing ability to create millions of different antibodies with different specificities. It is their ability
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IgM ELISA For the quantitative determination of IgM in human serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calibrator
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KAMIYA BIOMEDICAL COMPANY Mouse C-Peptide I EIA For the quantitative determination of C-Peptide I in mouse plasma, serum and urine. Cat. No. KT-367 For Research Use Only. 1 Rev. 091207 PRODUCT INFORMATION
HiPer Ion Exchange Chromatography Teaching Kit Product Code: HTC001 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 5-6 hours Storage Instructions: The kit is stable for
ELISA BIO 110 Lab 1 Immunity and Disease Introduction The principal role of the mammalian immune response is to contain infectious disease agents. This response is mediated by several cellular and molecular
FluoroTag FITC Conjugation Kit Product Number FITC1 Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description The FluoroTag FITC Conjugation Kit is suitable for the conjugation of polyclonal and
Serology: Fluorescent antibody tests and other tests employing conjugated antibodies Authors: Adapted by Prof M van Vuuren. Originally compiled by Dr RW Worthington. (Retired) Licensed under a Creative
489 18 Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan 18.1 Introduction Antibody catalysis is an experimental science. Although success in this field is
Grundlagen und Anwendung der Genom- und Proteomforschung Profilmodul (Vorlesung/Seminar WS 2012/13) Philipps-Universität Marburg Fachbereich Biologie Professor Dr. Egon Amann & Dr. Frank Vitzthum Gastbeiträge
Nitrotyrosine Western blot starter pack Cat no. A010-513 A convenient reagent pack containing Badrilla s high integrity nitrotyrosine monoclonal antibody (clone 2E11) and associated positive and negative
Chem 405 Biochemistry Lab I Experiment 2 Quantitation of an unknown protein solution. Introduction: The determination of protein concentration is frequently required in biochemical work. Several methods
Monash Antibody Technologies Facility The Monash Antibody Technologies Facility: A high throughput monoclonal antibody production platform for phospho-kinomics Tecan Symposium Zürich September 2007 What
Custom Antibodies & Recombinant Proteins INTRODUCTION Custom services to meet your research and development requirements Improvements in health, medicine and diagnostics over the past century can be largely
A Novel Bioconjugation Technology for Assay Development and More! Presentation overview Who we are Solutions we provide for our customers Solulink s technology Linking system The Solulink advantage Applications
Rubisco; easy Purification and Immunochemical Determination Ulrich Groß Justus-Liebig-Universität Gießen, Institute of Plant Nutrition, Department of Tissue Culture, Südanlage 6, D-35390 Giessen e-mail:
Why would you need to purify protein? Methods for Working with Protein 1. Protein Isolation A. Selection of a protein source i. tissue and cell cultures (bacteria, yeast, mammalian, etc.) ii. genetically
Anti-ATF6 α antibody, mouse monoclonal (1-7) 73-500 50 ug ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress.
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is a is a state of the art transfection reagent, specifically designed for the transfer of sirna and mirna into a variety of eukaryotic cell types. is a state of the art transfection reagent, specifically
Protein Purification and Analysis Numbers of genes: Humans ~40,000 genes Yeast ~6000 genes Bacteria ~3000 genes Solubility of proteins important for purification: 60-80% soluble, 20-40% membrane Some proteins
Principles of Immunohistochemistry Queen s Laboratory For Molecular Pathology Table of Contents POLYCLONAL VERSUS MONOCLONAL ANTIBODIES...PAGE 3 DIRECT & INDIRECT ASSAYS.PAGE 4 LABELS..PAGE 5 DETECTION...PAGE