A X S831 S antibacterial peptide agent ABPA Chinese Journal of Animal Nutrition

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1 Chinese Journal of Animal Nutrition doi /j. issn x * /2 Ⅰ Ⅱ Ⅲ + 90 d Ⅱ Ⅲ Ⅰ P > Ⅰ P < 0. 0 Ⅱ Ⅰ P < Ⅲ Ⅰ P < Ⅱ Ⅲ P > Ⅱ Ⅰ P < 0. 0 Ⅲ P < P > S83S86. 7 A X antibacterial peptide agent ABPA 3 AA Bao Y7 200B guoguotravel@ yahoo. cn * jzhang7576@ gmail. com

2 4 723 W 2 % = W - W 2 / W HE tachyplesin Ⅰ. 5 Excel 2003 SPSS 3. 0 One-w ay ANOVA LSD 450 P < P < ± /2 Ⅰ Ⅱ Ⅲ 2 0. g g 2 Ⅱ Ⅰ d % P > Ⅱ Ⅲ Ⅰ % P < % P < 0. 0 Ⅰ % % P < Ⅰ 0 / % 3. 78% 0. 66%. 65% 5. 3% 8 h % P > Ⅲ Ⅰ 0. 63% P > Ⅱ Ⅲ Ⅰ 3 Ⅱ Ⅲ 8. 85% 2. 53% 2. 53%. 07%. 07% Ⅲ Ⅰ 2. 7%. 4. P > Ⅱ Ⅲ OPTO-Start Ⅰ 6. 25% 8. 75% 0. 9% 9. 09%. 03%. 03% 3. 6% 4 d 3. 0% Ⅱ Ⅰ 2. 4% P > Ⅱ Ⅲ W Ⅰ 8. 75% P < % P < % 6. 67% P < 4 24 h % 0. 07% P < 0. 0 Ⅱ Ⅲ

3 Ⅰ 3. 8% P > % P < % P < Ⅰ. 03% 0. 5% % P > Ⅱ Ⅰ 0. 97% 2. 4% Ⅰ 6. 25% P > Ⅲ Ⅰ 2. 50% P > Ⅱ Ⅲ P > Table Composition and nutrient levels of the basal diets air-dry basis % Items ~ 2 to 2 days of age 22 ~ to 90 days of age Ingredients Corn Dehulled soybean meal Rice bran 4. 7 Wheat middling Imported fish meal Corn gluten meal Limestone CaHPO Soybean oil. 00 NaCl Lys 0. Met 0. 0 Premix Total Nutrient levels ME /MJ /kg CP EE CF Ash Ca TP Lys Met Met + Cys Thr Try Premix provided the follow ings per kg of diet ~ 2 to 2 days of age VA IU VD IU VE IU VK mg VB 5. 0 mg VB mg VB mg VB mg nicotinic acid mg biotin mg folic acid. 9 mg pantothenic acid mg Fe mg Mn 0. 0 mg Zn mg Cu 500 mg I 0. 5 mg Se 0. 3 mg22 ~ to 90 days of age VA 400 IU VD IU VE IU VK 3. 7 mg VB 4. 8 mg VB mg VB mg VB mg nicotinic acid mg biotin mg folic acid. 09 mg pantothenic acid mg Fe mg Mn mg Zn 0. 0 mg Cu 4. 0 mg I mg Se mg

4 4 725 Table 2 2 Effects of ABPA on meat quality in breast muscle of barred plymouth rock chickens Items ⅠGroup Ⅰ ⅡGroup Ⅱ ⅢGroup Ⅲ Meat color ± ± ± Drip loss /% ± ± ± Muscle fiber density //mm ± Aa ± ABb ± Bb Muscle fiber diameter /μm ± Aa ±. 48 Bb ± Bb P < P < 0. 0 P > In the same row values w ith different small letter superscripts mean significant difference P < and w ith different capital letter superscripts mean significant difference P < 0. 0 w hile w ith the same or no letter superscripts mean no significant difference P > The same as below. 3 Table 3 Effects of ABPA on free amino acid contents in breast muscle of barred plymouth rock chickens fresh sample basis % Items ⅠGroup Ⅰ ⅡGroup Ⅱ ⅢGroup Ⅲ Asp. 85 ± ± ± Thr * ± ± ± 0. 0 Ser ± Aa ± ABb ± 0. 0 Bb Glu ± ± ± 0. 3 Gly ± ± ± 0. 0 Ala. 05 ± a. 09 ± 0. 0 ab. ± b Cys 0. 6 ± ± ± Val *. 3 ± ± ± Met * ± ± ± 0. 0 Ile * ± ± ± 0. 0 Leu *. 66 ± ± ± 0. 0 Tyr ± Aa ± 0. 0 Bb ± 0. 0 Bb Phe * ± ± ± 0. 0 Lys *. 87 ± ± ± 0. 0 His ± ± ± 0. 0 Arg. 49 ± 0. 0 Aa. 34 ± 0. 0 Bb. 34 ± Bb Pro ± a. 03 ± 0. 0 b. 0 ± ab Essential amino acids ± ± ± Total ± ± ± * Essential amino acids for body % 7. 74% Ⅲ Ⅰ % % P > Ⅱ Ⅱ Ⅲ Ⅰ % Ⅰ 7. 56% Ⅲ Ⅰ 7. 78% P > Ⅱ Ⅲ % P < Ⅱ Ⅲ Ⅰ Ⅰ 7. 98% 3. 28% 3. 03% 3. 03% % P > Ⅱ Ⅰ P < 0. 0 Ⅲ Ⅰ 6. 67%. 49% P >

5 Table 4 Effects of ABPA on microelement contents in breast muscle of barred plymouth rock chickens fresh sample basis mg /kg Items ⅠGroup Ⅰ ⅡGroup Ⅱ ⅢGroup Ⅲ Ca ± ± ± P ± ± ± Zn ± ± ±. 37 Fe ± ± ±. 36 Cr ± ± ± Se ± Aa ± 0. 0 Bb ± Bb Cu 0. 4 ± ± ± Mn 0. 7 ± 0. 0 a 0. 7 ± a 0. 2 ± 0. 0 b Mg ± ± ± α

6 J J J BAO H SHE R LIU T et al. Effects of pig antibacterial peptides on grow th performance and intestine mu- cosal immune of broiler chickensj. Poultry Science D J J J. / J α - J D D J J J

7 Effects of Antibacterial Peptide Agent on Meat QualityContents of Free Amino Acids and Microelements in Breast Muscle of Barred Plymouth Rock Chickens GUO Lijun NIU Shuling MA Qian ZHAO Yantong BAI Mingna HAN Wenyu FENG Xin WANG Guiping 2 ZHANG Jing *. College of Animal Science and VeterinaryJilin UniversityChangchun 30062China 2. G uangdong Modern Agricultural G roup Co.Ltd.G uangzhou 50630China AbstractThis study w as conducted to investigate the effects of antibacterial peptide agent ABPAon meat qualitycontents of free amino acids and microelements in breast muscle of barred plymouth rock chickens. U- sing a single factor designa total of 450 one-day-old barred plymouth rock chickens w ere randomly allocated to 3 groups w ith 3 replicates per group and 50 chickens half male and half femalein each replicate. The experiment lasted for 90 days. Chickens in group Ⅰ control groupw ere fed a basal dietand the other chickens in groups Ⅱ and Ⅲ w ere fed w ith the basal diet supplemented w ith antibacterial peptide ABPand antibacterial peptides plus yeast ABPY respectively. The results show ed as follow s compared w ith the control groupdrip loss in groups Ⅱ and Ⅲ had a decrease tendencybut there w as no significant difference P > The muscle fiber diameter in groups Ⅱ and Ⅲ w as significantly low er than that in control group P < The muscle fiber density in groups Ⅱ and Ⅲ w as significantly higher than that in control group P < 0. 05P < The contents of essential amino acids and total free amino acids in breast muscle in groups Ⅱ and Ⅲ had no significant difference compared w ith the control group P > The selenium content in groups Ⅱ and Ⅲ w as significantly higher than that in the control group P < 0. 0 and the manganese content in group Ⅲ w as significantly higher than that in the control group P < but the other microelement contents in groups Ⅱ and Ⅲ had no significant difference compared w ith the control group P > In summaryabpa has positive effects on meat qualitycontents of free amino acids and microelements in breast muscle of barred plymouth rock chickenand the use of ABPA is better than the using ABP a- lone. Chinese Journal of Animal Nutrition Key wordsantibacterial peptideyeastbarred plymouth rock chickenmeat qualityfree amino acidmicroelements * Corresponding authorassociate professor jzhang7576@ gmail. com

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