Con struction of a M olecular Genetic M ap for M elon ( C ucum is m elo L. ) Ba sed on SRAP

Transcription

1 2007, 34 (1) : Acta Horticulturae Sinica F 2 SRAP 13, 1, 2, 1, 1, 2 ( 1, ; 2, ) : 4G21 ( ) 3A832 ( ) 114 F 2, 29 SRAP, 187, , cm, cm 6 32, cm, cm : ; SRAP; : S 65211; S 652 : A : X (2007) Con struction of a M olecular Genetic M ap for M elon ( C ucum is m elo L. ) Ba sed on SRAP WANG J ian2she 13, YAO J ian2chun 1, 2, L IU L ing 1, WANG Yong2jian 1, and L IW ei 2 ( 1 N ational Engineering Research Center for V egetables, B eijing , China; 2 Gansu A gricultural U niversity, Lanzhou , China) Abstract: A molecular map for melon (Cucum is m elo L. ) was constructed with SRAP markers using a population consisting of 114 F 2 individuals derived from the cross of 4G21 ( C. m elo var. chinensis) and 3A832 ( C. m elo var. saccherinus). Twenty2nine p rimer pairs were used and p roduced 187 polymorphic loci. The map consists of 12 linkage group s, which include 152 genetic markers, and covers cm w ith an average genetic distance of cm. Every linkage group has 6-32 genetic m arkers, average genetic dis2 tance is cm, Key words: M elon; Cucum is m elo L. ; and the length of linkage group is cm. SRAP; Molecular map, (Cucum is m elo L. ) Pitrat (1991) F 2 28, ,, Baudracco2A rnas Pitrat (1996) F 2 1, (W ang et al., 1997) F 2 (L iou et al., 1998; B rotman et al., 2000, 2002; O liver et al., 2001; Danin et al., 2002; Silber2 stein et al., 2003) 6, (R IL S ) ( P rin et al., 2002) (DH) : ; : : (863) (2002AA244021) ; ( H ) ; ( ) 3 E2mail: wangjianshe@ nercv1com (Danin2Poleg et al., 2001), (Danin2Poleg et al., 2001; P rin et al., 2002),,

2 SRAP ( Related sequence amp lified polymorphism, ) PCR, (L i & Quiros, 2001; Ferriol et al., 2003; L i et al., 2003) 4G21 ( ) 3A832 ( ), F 2 SRAP, G21 (, ) 3A832 (, ), F , CTAB (Murry & Thompon, 1980) DNA L i Quiros (2001) SRAP, 64 SRAP2PCR 10 L, DNA 50 ng; 50 ng; 100 mol/l dntps; 1 Taq DNA 015 U Taq DNA : 94 3 m in; s, s, 72 1 m in, 5 ; s, s, 72 1 m in, 35 ; m in PCR 2 L (98%, 10 mmol/l EDTA, 01025%, 01025% ), 95 5 m in, 6% PAGE (7 mol/l ) 1 TBE 80 W 1 h, 65 W 2 h, Bassam (1991) 113 SRAP, F 2 4G21 a, 3A832 b, - +, me2em121 me2 em11 Mapmaker/exp310group (LOD = 310, 3712 cm ), ripp le, try kosambi, ( cm ), SRAP, F , ; 1 15 ; 6145 ; 187, 96, 4817%, 91, 5113%, 1 1 ( P = 01796), 212 SRAP F 2 SAS , 8,, 4127% 8, 2 (me45em3221, me45em3225 ) P < 0105 ( (1) = 3184 ) ; 6 ( me45em122,

3 1 : F 2 SRAP 137 me45em3223, me45em3224, me45em4525, me45em3227, me45em3228) P < 0101 ( (1) = 6163 ) , 1817%, 34, 1 (me45em4525) Primer pair D istribution of polymorphism 3A832 4G21 1 Table 1 D istr ibution of polym orph ic loci in mapp ing paren ts Number of polymorphism Primer pair D istribution of polymorphism 3A832 4G21 me1em me17em me2em me21em me3em me21em me3em me21em me3em me21em me3em me45em me10em me45em me10em me45em me12em me45em me12em me45em me45em me46em me45em me49em me45em me49em me45em me49em me45em Average Total Number of polymorphism SRAP, 35, ( 1) 1, ;, 152 SRAP 2 L inkage group Length ( cm) 2 SRAP Table 2 D istr ibution of SRAP marker on the map Number of markers Number of distorted markers Maximum distance ( cm) LG LG LG LG LG LG LG LG LG LG LG LG Amounts : 3, 3 3 P < 0105 P < (1) = 6163 Note: 3, 3 3 show P < 0105 and P < (1) = 6163, respectively. Average distance ( cm)

4 , SRAP cm, cm 6 32, cm, cm LG1 32, LG11 6 LG cm 152 SRAP 7, 416%, 4 (LG2, LG3, LG6 LG12), LG2 LG6 3 (me45em123, me45em3222, me49em3428) 2 (me45em3224, me45em3227),, 1 SRAP ( cm),, 3, 3 3 P < 0105 P < (1) = 6163 F ig11 M olecular genetic map of m elon ( Cucum is m elo L1) Absolute distance in cm is shown on the left side of linkage group s and locus names on the right; 3, 3 3 show P < 0105 or P < (1) = 6163, respectively.

5 1 : F 2 SRAP G21 ( ) 3A832 ( ), F 2 1 SRAP 311 SRAP, 29187, 1 15, 6145 Baudracco2A rnas Pitrat (1996) 13RAPD 16, 1123 ; L iou (1998) RAPD DNA, 60RAPD 125, 311,, SRAP 312 SRAP, 518% 1918% (Baudracco2A rnas & Pitrat, 1996; W ang et al., 1996; L iou et al., 1998; O liver et al., 2001; P rin et al., 2002) SRAP, 416%,, SRAP, 3,, ( Xu et al., 1997),,, 1, ( 1 2) (Haanatra et al., 1999),,,,, ( 1 2 ) (Haanatra et al., 1999), 8,,, F SRAP,, SRAP, cm, cm,,,, LG3, 5517 cm, cm (Baudracco2A rnas & Pitrat, 1996) QTL, 10 cm ; QTL, 1 cm O liver (2001) P rin (2002), 310 cm 215 cm,,,,,, ;,

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