Region 9/10 EPA/CDC Large Scale Exercise: August, 2010 The LRN-C Response
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1 Region 9/10 EPA/CDC Large Scale Exercise: August, 2010 The LRN-C Response Tracy Andacht, Ph. D. and Blaine Rhodes 2011 APHL National Meeting 8 June, 2011 National Center for Environmental Health Division of Laboratory Sciences
2 Exercise Goals Test specimen logistics Accessioning of large number of samples for all labs Logistics for handling large number of real world samples (i.e. urine cups) Packaging and shipping for L2 and L3 labs Test analytical capability of Level 1 and 2 laboratories Test communication between: LRN-C laboratories EPA and CDC networks Test data reporting between: LRN-C laboratories EPA and CDC networks
3 Chemical Laboratory Response Network 7 labs, packaging/shipping CDC Level 1 Level 2 Level 3 37 labs, 8 methods, P/S 10 labs 37 labs 13 methods 8 methods P/S 10 labs, 13 methods, P/S P/S 7 labs pkg/ship
4 The LRN-C Players WA OR ID CA NV VA LA-Co AZ CDC HI AK Level 1 Lab Level 2 Lab Level 3 Lab
5 Exercise Preparation 9 participating labs x 100 samples each 30 ml urine x 900 samples = 30 L urine!!!! 5 urine pools: 4 blank urine pools 1 urine pool for spiking 3 spike levels, 2 agents, 5 replicates 16 exposed samples/lab
6 Day t - 1 Samples Observers Samples and Observers
7
8 Specimen Packaging, Shipping, and Accessioning Successes: All packaged and shipped specimens arrived at proper temperature All Level 1 and 2 laboratories successfully handled receipt and acquisitioning of specimens Challenges: Broken evidence tape due to dry ice Security of specimens within building
9 Analytical Capability Capability Successes: No batch rejects based on bench QCs Good accuracy and precision for positive samples All positive for Total Arsenic by Level 2 labs (78), also confirmed positive for SBMTE and CVAA (CA-SPHL) 0.3% false positive for SBMTE (CA-SPHL) 0.3% false negative for CVAA (CA-SPHL) Bonus: expanded capabilities of Level 2 labs Network capabilities provided support when lab capabilities down
10 Analytical Capacity Capacity Successes: All Level 2 labs with capability were able to run and report results within 24 hour from receipt Level 1 labs provided mutual surge
11 Data Reporting Successes: Use of LRN Results Messenger by Level 2 and Level 1 programs to report jurisdictional sample data to the CDC Oregon LIMS and the 3-State MOA AK, ID, WA sent arsenic and CVAA result data to OR SPHL in a specified format so as to allow the data to be uploaded into the Oregon LIMS system CA-SPHL did the same when reporting SMBTE and CVAA confirmation results Challenges: Sharing data between Level 2 and Level 1 labs
12 The Unexpected: High Urine Arsenic Levels in Earmarked ND Samples CDC used 5 separate urine pools for exercise samples Urine pool #2 had an average arsenic level at 50 µg/l, the action level for confirmation shipping to Level 1 Lab 85% of Idaho and 10% of Nevada specimens contained urine from pool #2 The Geometric Mean of Total Arsenic In US Population is 8.30 µg/l (NHANES 4 th Report)
13 Lessons Learned Capability and capacity must be addressed up front on the first call Limitations in RM when reporting 2 nd party sample results to the primary party Contact information on LRN-C website not accessible by all Instrumentation software is in need of an upgrade
14 Thanks to.. Blaine Rhodes Washington Michael Stevenson Idaho Teresa McGivern Oregon David Quilici Nevada Jason Mihalic Arizona Alon Volner CA-LA County Bill Draper/Kevin Chao California David Verbrugge Alaska Al Asato - Hawaii Shane Wyatt Virginia And all the participants from the CDC.
15 Questions? For more information, please contact: Centers for Disease Control and Prevention National Center for Environmental Health 4700 Buford Highway, NE Atlanta, GA The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention. National Center for Environmental Health Division of Laboratory Sciences
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