School of Science SYLLABUS FOR. PROGRAM: B.Sc. Microbiology (BSM501) SEMESTER: V ACADEMIC YEAR: DIRECTOR SCHOOL OF SCIENCE RK UNIVERSITY

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1 School of Science SYLLABUS FOR PROGRAM: B.Sc. Microbiology (BSM501) ACADEMIC YEAR: SEMESTER: V DIRECTOR SCHOOL OF SCIENCE RK UNIVERSITY RAJKOT

2 Course Title MICROBIOLOGY Course Code BSM 501 Lecture : 4 Course Credit Practical : 3 Tutorial : 0 Course Learning Outcomes Total : 7 On the completion of the course, students will be able to: milk. Understand the basics concepts of applied microbiology Learn various techniques for qualitative analysis of milk and grading of Analyze the quality of water, food and milk Detailed Syllabus Sr. No. Name of Chapter & Details Hours Allotted Unit-1 Microbiology of Soil Section-I A. Properties of soil Physical & Chemical Characteristics of Soil, Rhizosphere & Microbial flora of Soil, Interactions among soil microorganisms: Neutral, Beneficial & Harmful interactions B. Biogeochemical cycles Introduction of Mineralization & immobilization of elements,

3 Unit-2 Unit-3 Nitrogen Cycle: Proteolysis, Ammonification, Nitrification, Nitrate reduction,, Denitrification; Biochemistry & genetics of nitrogen fixation (nif gene technology), Sulphur Cycle: Sulphur oxidation & Reduction, Winogradsky s Column, Carbon cycle: Degradation of complex organic compounds, formation of humus and its importance. Food Microbiology Microbial flora of fresh food, Microbial spoilage of foods: Fresh foods & Canned foods, Food Borne infection & intoxication: Role of S.aureus, C.botulinum & Salmonella Spp..in food poisoning, Preservation of foods: General principles & methods of food preservation, Microbiological examination of food; Introduction to AGMark, Brief introduction about fermented foods: Pickles, Sauerkraut, Silage, Sausages & Bread, Microorganisms as food: Single Cell Protein, Mushrooms and Functional foods Dairy Microbiology Milk as a medium, normal flora of milk, Types of microorganisms in milk: Biochemical types, Pathogenic types, Temperature types; Spoilage of milk & milk products, Microbial analysis of milk: SPC, Direct count, MBRT, Resazurin test, Grading of milk, Fermented milk Beverages & Manufactured Dairy Products: Starter Culture, Cheese, Yogurt, Buttermilk, Acidophilus milk, Kefir, Preservation of milk: Principles & methods of preservation Section-II Unit-4 Aquatic Microbiology & Microbiology of waste water A. Introduction Natural Water: Atmosphere, surface, Stored & Ground water; Aquatic environment: Temperature, Hydrostatic pressure, Light, Salinity, Turbidity, ph, inorganic & organic constituents, Distribution of microorganisms in the aquatic environment

4 Unit-5 B. Microbiology of drinking water Sanitary survey, Bacteriological evidence of pollution, Bacteriological analysis & Sampling techniques of water, Microorganisms other than Coliforms as nuisance organisms C. Water purification Sedimentation, Filtration use of Sand filters, Disinfection D. Waste water Chemical and Microbial Characteristics of waste water, B.O.D., C.O.D. as indicator of quality of waste water, Waste water treatment & Disposal --Single Dwelling Process & Municipal Treatment -- Primary Treatment, Secondary Treatment, Advanced & final treatment, Solid waste processing: Anaerobic Sludge digestion & Composting Environment and Biotechnology A. Environment Types of Pollutants, Sources & Effect on ecology, Pollution by pesticides, Biomagnifications of pesticide & their biological control, Brief account on Water pollution (by Oil, Detergent, Heavy metal & industrial effluent) & their Biological control, Role of microorganisms in Biodeterioration of Paper, Textiles, paints, woods & metals and their control, Air Pollution & Air Sanitation B. Biotechnology What is Biotechnology, Biotechnology as interdisciplinary science; Ethical, Legal and Social Implications of Biotechnology; Bioleaching, Microbial enhanced oil recovery; Biofuels, Bioplastics FUNDAMENTALS OF MICROBIOLOGY (PRACTICAL) 6 HOURS PER WEEK 1. Isolation of nitrogen fixing bacteria 2. Cultivation of nitrifying and denitrifying bacteria (Demo) 3. Cultivation of cellulose decomposing microorganisms from soil((demo)

5 4. Demonstration of oozing, and isolation of pathogen from diseased specimen of lemon leaf showing citrus canker and isolation of Xanthomonas spp. 5. Standard qualitative analysis of milk 6. Methylene Blue Reduction Time test for milk 7. Estimation of DO. 8. Isolation and identification of coli forms from Water by Presumptive, Confirmed & Completed test Instructional Method and Pedagogy: Lectures will be conducted with the aid of multi-media projector, black board, OHP etc. Assignments based on course content will be given to the students at the end of each unit/topic and will be evaluated at regular interval. Surprise tests/quizzes/tutorials will be conducted. The course includes a laboratory, where students have an opportunity to build an appreciation for the concepts being taught in lectures. Minimum ten experiments shall be there in the laboratory related to course contents. Reference Books: 1. Principles of Microbiology By Atlas R.M.: 2nd edition 2. Microbiology by Pelczar M.J. & Chain E.C.S. : 5th edition 3. Introduction to soil microbiology by Alexander M: 2nd edition 4. Biotechnology fundamental & applications By Purohit S.S. 5. Diseases of Crop plants in India by Rangaswami G. 6. Microbiology fundamental & applications By Purohit S.S. 7. Microbiology by Prescott L.M. 8. Fundamentals of Microbiology By Frobisher M.: 9th edition 9. Industrial Microbiology by Prescott S.C. : 3rd edition 10.Food Microbiology by Frazier W.C.: 3rd edition

6 11. Food science & Experimental foods By Swaminathan M... Modern food microbiology by J James 13. Environmental microbiology by Rania Maier, Academic Press 14. Advanced Waste water Treatment by R.K. Goel 15. Fundamentals of Dairy Microbiology by Prajapati J.B 16. Biotechnology by Trevan M.D. 17. Biotechnology by Smith, Oxford 18. International student edition: Microbiology- A laboratory Manual 4 th edition. By James G. Chappuccino & Natalie Sherman 19. Bacteriological Techniques By F.J. Baker 20. Introduction to Microbial Techniques By Gunasekaran. 21.Introductory Practical Biochemistry by S.K.Sowhney & Rundhir Singh 22. Standard Methods for Examination of waste water L.S. Clesceri, A.E.G reenberg & A.D. Eaton APHA 1998.