ATGCell. ATGCell Inc.

Transcription

1 ATGCell Inc.

2 Corporate ATGCell is a revenue generating spin-off biotech company from University of Alberta. Incorporated in September 2007; 6 employees (5 PhD and 1 MSc); several professional consultants Located in Alberta Research Council. These GLP laboratories are equipped for standard molecular biology and small protein production works. We specialize in protein production and purification using mammalian cells. We also have certain expertise in bacterial expression and purification. Main customers: pharma and biotech companies.

3 Our Technology Robust Exogenous Expression in MAmmalian Cells (REEMAC). A platform technology which can significantly increase protein expression level in mammalian cells, and allows for rapid expression of many difficult-to-express proteins. This platform can be used for protein production (reagent or protein therapeutics) and drug target over-expression for screening. Patent pending. ExpressBoost reagents. Custom formulated reagents which further enhance exogenous expression levels in mammalian cells by up to 20 folds in transient or stable transfection events. Bacterial Expression System.

4 NanoEngineering on Expression Vectors The following DNA elements were engineered: Promoter Untranslated regions (UTRs) Expression cassette structure Special expression elements Selection marker Secreting lead signal and 5 and 3 UTR Host cell lines Culture conditions Gene delivery methods

5 Applications of Optimized REEMAC Technology Our optimized REEMC Technology has been successfully used in : Protein production for: Naturally secreting proteins Non-secreting proteins Antibodies Membrane proteins Functional stable cell line generation: Membrane proteins Cytoplasmic proteins Toxic proteins Proteins with large size

6 Proteins Expressed Using REEMAC The following proteins have been successfully expressed using REEMAC technology: Cytokines: 34 Growth factors: 24 Cytokine receptors and cell surface antigens: 26 Proteases and regulators: 10 Peptide hormones: 5 Protein kinases: 4 G-protein coupled receptors: 10 Nuclear receptors: 2 Ion channels: 5 Transporters: 5 Chimera proteins: 5 Monoclonal antibodies: 4

7 Production of Research Reagent Proteins 1: Invitrogen psectaq2 vector 2: REEMAC1 vector A: viral protein, 70 kd B: human protein, 30 kd Both viral and human proteins can be efficiently expressed in CHO cells using REEMAC A B

8 Production of Research Reagent Proteins (conc.) 1: Invitrogen psectaq2 vector 2: REEMAC1 vector C: Plant protein 1, 35 kd D: Plant protein 2, highly glycosylated, 100 kd, multiple bands were caused by different glycosylation status C D Plant proteins can be efficiently expressed in CHO cells using REEMAC.

9 Production of Preclinical Materials Secreting protein X: two-domain hormone-like fusion drug candidates X1: 50 kd; X2: 65 kd Failed to produce biologically active protein using bacterial expression. Custom production yield: µg/l Supernatant H: HEK-293 C: CHO-K1 H C H C X1 X2 Biological active proteins; 5 mg/l for cell line pool; Stable cell line pool generation: 3 weeks ATGCell

10 Production of Preclinical Materials (conc.) Non-Secreting protein Y: Novel human protein, 60kD Failed to produce biologically active protein using bacterial expression. Supernatant (1/100) CHO-K1 Evaluation (yield and biological activity) is ongoing; Stable cell line pool: 3 weeks; Single clones: 4 weeks Cell lysate (1/5)

11 Stable Cell Line Generation Platelet-activating factor receptor (PTAFR) NM_ Neuropeptide Y receptor Y1 (NPY1R) NM_ Adenosine A1 receptor (ADORA1) NM_ Angiotensin II receptor, type 2 (AGTR2) NM_ ATGCell

12 Main Features of REEMAC Allow quick generation of highly expressed stable cell lines. Consistent and high expression level and efficient secretion. Simple collection of secreted proteins from culture media for downstream processing. 2-3 different genes can be simultaneously expressed. Real-time expression monitoring. Low Cost and Time Savings

13 Services and Products ATGCell is committed to rapidly providing protein expression and purification, or stable cell line development services at low cost: Protein targets: antibodies, growth factors, cytokines, membrane-bound proteins and others Milligram of proteins can be produced in less than 10 weeks. Stable cell lines can be generated in 7 weeks. Custom special services available upon request. No upfront payment for services Royalty free technology out licensing Flexible working option Short production time

14 Services and Products (conc.) There are several advantages associated with our products/services: Fully functional proteins expressed in CHO cells for ease of clinical regulatory approvals Faster turn around time for protein production translates to cost advantages in time to market High purity research reagent protein production capacity with lower price points us a favourable condition to market competitors.

15 Services and Products (conc.) ATGCell is selling/distributing the following research reagent products at reasonable price and high quality: DNA transfection reagent ExpressBoost reagents DNA amplification reagents Recombinant proteins: growth factors, cytokines Stable cell lines: membrane-bound proteins Viral proteases Protein/antibody purification resins Antibodies

16 Product Catalogue Please visit our Website for full access to our product catalogue, list of services and technical advancement.

17 Acknowledgements ATGCell