Additional file 1 - Collection details of the sloth samples which sequence data was used in the

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1 Additional file 1 - Collection details of the sloth samples which sequence data was used study and the number of clones sequenced from each sample. Sample number Sloth species Sloth details Collection place Collection date Algal observations Clones sequenced A101 roadkill Panamerican Highway between Arraiján and Panama City S2 Panama S3 Panama microscope 3 s4m Panama microscope 1 S6 Panama s6.1 baby of s6 Panama S7 Panama S8 5,8 kg Island, Panama microscope 9 S9 5,2 kg Island, Panama microscope 3 1

2 s11 almond del Toro, Panama green 3 s12b almond del Toro, Panama green 10 s15 del Toro, Panama green 4 almond s16 on the shore del Toro, Panama green 4 s18 almond del Toro, Panama green 18 s32 almond del Toro, Panama green 12 small individual, s33 almond del Toro, Panama green 7 2

3 s34 sb1 sb3 sjb COS1 almond del Toro, Panama green 12 Island, Panama green 16 Island, Panama Manuel Antonio, old male Costa Rica Mar green 5 Korkeasaari zoo, zoo Helsinki, Finland - - baby Costa Rica CHD 108 CHD didactylus zoo Cayenne, French Guiana Cayenne, French didactylus Guiana Algae visible female tridactylus subadult French Guiana microscope - tridactylus female adult French Guiana male adult, tridactylus wounded French Guiana month old tridactylus baby French Guiana

4 striw JB812 JB830 s5 s10 hair dark, tridactylus female subadult, forest site French Guiana microscope 1 female adult, tridactylus forest site French Guiana tridactylus French Guiana tridactylus male French Guiana microscope 47 tridactylus French Guiana tridactylus French Guiana tridactylus Brazil, Amazonia 1977 microscope - male adult Costa Rica Sept green 42 electrocuted sloth Costa Rica Nov.2005 green 1 Panama microscope - 3,1 kg Island, Panama microscope 4 4

5 s13k del Toro, Panama microscope 3 female, on s14 the ground del Toro, Panama green 3 s14.1 baby of s14 del Toro, Panama female, Cecropia- s17 del Toro, Panama green 2 s17.1 baby of s17 del Toro, Panama microscope - male, s30 del Toro, Panama green 11 female adult, s31 del Toro, Panama green 1 baby of s31, s31.1 del Toro, Panama sb2 female Island, Panama microscope 5 sb5 Island, Panama sb6 sick female Island, Panama green 4 5

6 sick baby of sb6.1 sb6 Island, Panama microscope - small individual, s19 green 9 near to s19, s20 green 14 female adult, s21 green 8 baby of s21, s21p - 7 female, s22 green 6 female, s23n green 11 male, s24 green 6 female, s25 green 11 6

7 female, s26 green 13 small individual, s27 green 1 small individual, s28s microscope 15 male, s29 green 10 Augusto Ruschi Biological Reserve, Santa adult male Teresa, Espirito BT 53 6,7kg Santo, Brazil microscope 6 Augusto Ruschi Biological Reserve, Santa adult female Teresa, Espirito BT 54 9,2kg Santo, Brazil microscope 7 Augusto Ruschi Biological Reserve, Santa adult male Teresa, Espirito BT 56 5,7kg Santo, Brazil microscope 12 7

8 Paulo Seike adult female BT 57 9,5kg Brazil microscope 10 Paulo Seike adult female BT 58 7,1kg Brazil microscope 6 Paulo Seike BT 59 infant 0,5kg Brazil Paulo Seike adult female BT 60 7,3kg Brazil microscope 7 Paulo Seike adult male BT 61 7,6kg Brazil microscope 11 8

TABLE 2 CONSANGUINEOUS MARRIAGE IN THE AMERICAS

TABLE 2 CONSANGUINEOUS MARRIAGE IN THE AMERICAS Region/country Location Collection Study Sample Consanguinity Consanguinity Coefficient of Reference period population size (%) types inbreeding (α) Caribbean