INNOVATIONS IN RECIRCULATING INLAND MARINE AND FRESHWATER FISH PRODUCTION SYSTEMS

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1 INNOVATIONS IN RECIRCULATING INLAND MARINE AND FRESHWATER FISH PRODUCTION SYSTEMS Kevan L. Main, Jim Michaels, Michael Nystrom Mote Marine Laboratory, Sarasota, Florida, USA & Thomas Losordo North Carolina State University, Raleigh, North Carolina, USA

2 Outline of Presentation Overview of Mote Aquaculture Park Research Programs, Facilities & Systems Overview of Denitrification Research Freshwater & Marine Prototype System for Fingerling Production System overview & Preliminary Operation

3 Aquaculture Research at Mote Focused on Developing & Demonstrating Recirculating Aquaculture System (RAS) Technology Concerns about pollution of marine & freshwater resources High cost of coastal land Limited water resources Efficient use of water is needed to sustainability grow commercial aquaculture in the U.S.

4 Mote Aquaculture Park: 2009 Maintenance & Shop Storage Sturgeon GO-III Sturgeon Hatchery & Office Sturgeon GO-I Sturgeon GO-II Marine Broodstock Marine Fish Hatchery Sturgeon Processing Marine Filtration Sturgeon Purge Red Drum Hatchery Snook Pond

5 Sturgeon Commercial Demonstration Program Demonstrate commercial viability of sustainable aquaculture farming in Florida Develop innovative culture techniques to protect threatened sturgeon stocks Develop technologies to conserve limited water resources

6 Larval Rearing Tanks & Filtration

7 Fingerling Tanks & Filtration

8 Sturgeon Growout Tanks

9 Solids Filtration Microscreen Drum (removes particles/solids greater than 60 microns)

10 Biofiltration Moving Bed Reactor (nitrification chamber)

11 Degassing Tank CO2 Stripping

12 Oxygenation

13 Marketing Sustainable Seafood Products to U.S. Consumers Siberian Sturgeon & Caviar from Mote Aquaculture Park Fish You Can Feel Good About

14 Marine Aquaculture Research Program Design & test zero-discharge, sustainable marine farming systems for inland applications Demonstrate the use of these technologies to culture marine fish (pompano, snook, red drum) and invertebrates (hard corals, sea urchins) for food & stock enhancement

15 Marine Fish Breeding Systems Large breeding tanks Independent temperature, lighting and filtration

16 Marine Fish Broodstock System Small Scale Moving Bed Reactor Foam Fractionator Drop Filter for Solids Removal UV Filter 4/17/2009

17 Marine Hatchery/Nursery Facilities

18 Denitrification Research Freshwater Phase I Evaluate the performance of the denitrification reactor Evaluated 4 carbon sources (vinegar, molasses, starch, methanol) for viability and comparative performance Phase II Determined that molasses was the most viable carbon source option under commercial conditions in our location Capital cost savings were 27% higher & operating cost savings were 87% higher for molasses vs. other carbon sources Marine Phase I Trial Evaluate the efficiency of a denitrification reactor in removing nitrate in a low salinity (15-18 ppt) wastewater treatment system Carbon source Methanol Maximum water flow rate = 6 L/min: 12 ml/min carbon (15% methanol)

19 Commercial-Scale Freshwater Denitrification Filter Carbon Sources

20 Preliminary Results Denitrification Filter Trial Low-Salinity Wastewater Treatment System Carbon Source (Methanol Tank) Biofilte Tank Denitrification Reactor Started methanol 2ml/min Increased water flow to 2L/min Increased water flow to 3L/min Increased methanol flow to 6ml/min During this time the pump was slowly decreasing due to a clo and reduced the flow to 3.0L/min NO3 mg/l Started Water 1L/min Increased water flow to 3.8L/min Water flow was restored to 4.0L/min NO3 Before Reactor NO3 Post Reactor Denitrification Reactor Outlet Weeks

21 Florida Marine Fisheries Enhancement Initiative Designing, constructing & evaluating system performance of a prototype system to produce fingerling fish for stock enhancement Evaluating Red Drum performance (growth, survival, health) in intensive recycle systems

22 Prototype Zero- Discharge Fingerling Production System

23 Filtration System Components

24 Wastewater Treatment Components

25 Acknowledgements FL Department of Agriculture & Consumer Services Division of Aquaculture USDA/CSREES FL Fish and Wildlife Conservation Commission Southwest Florida Water Management District Darden Restaurants Foundation Mote Scientific Foundation