COULTER FlowCentre Multimedia Workstation. Getting Started. PN EA (July 2010) Beckman Coulter, Inc. 250 S. Kraemer Blvd.

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1 COULTER FlowCente Multimedia Wokstation Getting Stated (July 2010) Beckman Coulte, Inc. 250 S. Kaeme Blvd. Bea, CA 92821

2 WARNINGS AND PRECAUTIONS READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER S RECOMMENDATIONS. IF IN DOUBT AS TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE. HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS WARNINGS, CAUTIONS, and IMPORTANTS alet you as follows: WARNING - Can cause injuy. CAUTION - Can cause damage to the instument. IMPORTANT - Can cause misleading esults. BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO, PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR ANY OTHER AUTOMATED LABORATORY ANALYZER. WARNING Risk of opeato injuy if: All doos, coves and panels ae not closed and secued in place pio to and duing instument opeation. The integity of safety intelocks and sensos is compomised. Instument alams and eo messages ae not acknowledged and acted upon. You contact moving pats. You mishandle boken pats. Doos, coves and panels ae not opened, closed, emoved and/o eplaced with cae. Impope tools ae used fo toubleshooting. To avoid injuy: Keep doos, coves and panels closed and secued in place while the instument is in use. Take full advantage of the safety featues of the instument. Do not defeat safety intelocks and sensos. Acknowledge and act upon instument alams and eo messages. Keep away fom moving pats. Repot any boken pats to you Beckman Coulte Repesentative. Open/emove and close/eplace doos, coves and panels with cae. Use the pope tools when toubleshooting. CAUTION System integity might be compomised and opeational failues might occu if: This equipment is used in a manne othe than specified. Opeate the instument as instucted in the Poduct Manuals. You intoduce softwae that is not authoized by Beckman Coulte into you compute. Only opeate you system s compute with softwae authoized by Beckman Coulte. You install softwae that is not an oiginal copyighted vesion. Only use softwae that is an oiginal copyighted vesion to pevent vius contamination. IMPORTANT If you puchased this poduct fom anyone othe than Beckman Coulte o an authoized Beckman Coulte distibuto, and, if it is not pesently unde a Beckman Coulte sevice maintenance ageement, Beckman Coulte cannot guaantee that the poduct is fitted with the most cuent mandatoy engineeing evisions o that you will eceive the most cuent infomation bulletins concening the poduct. If you puchased this poduct fom a thid paty and would like futhe infomation concening this topic, call you Beckman Coulte Repesentative.

3 REVISION STATUS Initial Issue, 7/97 Revision B, 5/98 Added infomation elated to the ALTRA flow cytomete. Revision C, 12/98 Added infomation elated to the XL /XL-MCL flow cytomete SYSTEM II softwae vesion 3.0. Revision D, 10/99 Added infomation elated to the latest compute and monitos, and Micosoft Windows 98. Revision E, 11/00 Added infomation elated to the latest compute and monitos, and the EXPO32 ADC softwae. Revision EA, 07/10 Updates wee made to the company copoate addess. Note: Changes that ae pat of the most ecent evision ae indicated in text by a ba in the magin of the amended page. This document applies to the latest softwae listed and highe vesions. When a subsequent softwae vesion changes the infomation in this document, a new issue will be eleased to the Beckman Coulte website. Fo labeling updates, go to and download the most ecent manual o system help fo you instument. i

4 REVISION STATUS ii

5 CONTENTS LEGAL NOTICES REVISION STATUS, iii OVERVIEW, 1 STARTING ALTRA OR ELITE SOFTWARE, 3 NOTES ON WORKING WITH ALTRA OR ELITE SOFTWARE AND WINDOWS 98 SE, 3 STARTING ALTRA OR ELITE SOFTWARE, 3 STARTING WIN 98 SE, 3 EXITING TO MS-DOS FROM ALTRA OR ELITE SOFTWARE, 3 RESTARTING ALTRA OR ELITE SOFTWARE FROM MS-DOS, 4 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM THE STARTUP MENU, 5 NOTES ON WORKING WITH XL/XL-MCL SYSTEM II SOFTWARE AND WINDOWS 98 SE, 5 STARTING XL/XL-MCL SYSTEM II SOFTWARE, 5 STARTING WIN 98 SE, 5 EXITING TO MS-DOS FROM SYSTEM II SOFTWARE, 6 RESTARTING XL/XL-MCL SYSTEM II SOFTWARE FROM MS-DOS, 6 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON, 7 NOTES ON WORKING WITH XL/XL-MCL SYSTEM II SOFTWARE AND WINDOWS 98 SE, 7 STARTING WIN 98 SE, 7 STARTING XL/XL-MCL SYSTEM II SOFTWARE, 7 EXITING TO WIN 98 SE DESKTOP FROM SYSTEM II SOFTWARE, 8 RESTARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON, 8 SYSTEM COMPONENTS, 9 CPU, 9 MEMORY, 9 INPUT DEVICES, 9 DATA STORAGE, 9 OPERATING SYSTEMS, 9 MONITOR, 9 v

6 CONTENTS INTERFACES, 9 OPTIONAL ACCESSORIES, 9 MAINTAINING SYSTEM PERFORMANCE, 11 SCANDISK, 11 DEFRAGMENTING, 12 DRIVESPACE:, 12 MEMMAKER, 12 ARCHIVING DATA, 13 CHANGING WIN 98 SE STARTUP MENU CRITERIA, 13 SOFTWARE SUPPORT, 15 WINDOWS 98 SE GUIDED TOUR, 15 WINDOWS 98 SE HELP SYSTEM, 15 MICROSOFT TECHNICAL SUPPORT, 15 BECKMAN COULTER SOFTWARE TECHNICAL SUPPORT, 15 TRADEMARKS vi

7 OVERVIEW This booklet intoduces you to you COULTER FlowCente Multimedia Wokstation. The FlowCente Multimedia Wokstation is a dual-bootable Wokstation that includes: Micosoft Windows 98, second edition (WIN 98 SE) opeating system. Micosoft MS-DOS 6.22 (DOS) opeating system. You FlowCente Multimedia Wokstation is configued to un COULTER EPICS XL Flow Cytomete SYSTEM II softwae, COULTER EPICS ALTRA softwae, o COULTER EPICS Elite softwae. The FlowCente Multimedia Wokstation can also use EXPO32 o EXPO32 ADC Cytomete softwae which, if installed, povides the solution to you data acquisition and analysis equiements in the Windows opeating system. Windows 98 SE has multi-tasking capabilities that let you acquie data and pint expeiment epots in the backgound, while simultaneously woking in anothe application. The esult is an incease in poductivity and ceativity fo data epoting, analysis and pesentations. The Windows envionment offes all the editing and online help tools that ae familia to you. See the documentation that accompanies you vesion of the EXPO32 o EXPO32 ADC softwae fo detailed instuctions how to use that softwae E 1

8 OVERVIEW 2

9 STARTING ALTRA OR ELITE SOFTWARE NOTES ON WORKING WITH ALTRA OR ELITE SOFTWARE AND WINDOWS 98 SE The ALTRA and Elite softwae have been validated to opeate in the MS-DOS opeating system only. Theefoe the softwae must opeate fom the MS-DOS opeating system and it cannot opeate in a Windows 98 SE envionment o an MS-DOS window fom Windows 98 SE. Do not launch ALTRA o Elite softwae fom an icon on the WIN 98 SE Desktop o fom the WIN 98 SE MS-DOS pompt. Duing system statup, the WIN 98 SE Statup Menu appeas. The menu displays 8 choices fo stating the opeating system including, 8. Pevious vesion of MS-DOS, (ALTRA o Elite softwae) and 1. Nomal, (WIN 98 SE opeating system). If you do not make a selection within 90 seconds, the system automatically stats WIN 98 SE. STARTING ALTRA OR ELITE SOFTWARE To stat ALTRA o Elite softwae, at the WIN 98 SE Statup Menu, pess 8 and then pess Û to select option 8. Pevious vesion of MS-DOS duing system bootup. STARTING WIN 98 SE To stat WIN 98 SE, at the WIN 98 SE Statup Menu, pess 1 and then pess Û to select option 1. Nomal. This loads the WIN 98 SE opeating system and displays the WIN 98 SE desktop. Please note that ALTRA and Elite softwae ae not accessible fom the WIN 98 SE opeating system. The EXPO softwae, if installed, is accessible fom the WIN 98 SE desktop. Micosoft Windows 98 Statup Menu 1. Nomal 2. Logged ( \BOOTLOG.TXT) 3. Safe Mode 4. Safe mode with netwok suppot 5. Step-by-step confimation 6. Command pompt only 7. Safe mode command pompt only 8. Pevious vesion of MS-DOS MENU D EXITING TO MS-DOS FROM ALTRA OR ELITE SOFTWARE Select Exit fom the Applications Menu to tun off the ALTRA o Elite softwae and ente the MS-DOS opeating system (see the MS-DOS manuals fo moe infomation on the MS-DOS opeating system). The Cytomete emains on. To etun to the WIN 98 SE opeating system, eboot the Wokstation (pess Ý+ Þ + á) and select option 1. To tun off the Cytomete and compute, tun off the main powe switch on the Cytomete. 3

10 STARTING ALTRA OR ELITE SOFTWARE RESTARTING ALTRA OR ELITE SOFTWARE FROM MS-DOS RESTARTING ALTRA OR ELITE SOFTWARE FROM MS-DOS To etun to the ALTRA o Elite softwae fom the MS-DOS opeating system, type CYTOMETE and pess Û at the C:\ALTRA> o C:\ELITE> pompt. Note: See the appopiate instument poduct manual fo the location of the powe switches. 4

11 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM THE STARTUP MENU NOTES ON WORKING WITH XL/XL-MCL SYSTEM II SOFTWARE AND WINDOWS 98 SE The XL/XL-MCL SYSTEM II softwae has been validated to opeate in the MS-DOS opeating system only. Theefoe, the softwae must opeate fom the MS-DOS opeating system and it cannot opeate in a Windows 98 SE envionment o an MS-DOS window fom Windows 98 SE. You Beckman Coulte Repesentative can install two XL SYSTEM II softwae icons on the WIN 98 SE desktop of you FlowCente Wokstation which can easily link to the MS-DOS opeating system and launch the SYSTEM II softwae. Note: If you Beckman Coulte Repesentative has aleady installed these two XL SYSTEM II softwae icons on the WIN 98 SE desktop of you FlowCente Wokstation, use the instuctions located in the next section: STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON. Use the instuctions in this section if you FlowCente Wokstation does not have the XL SYSTEM II softwae icons installed on the WIN 98 SE desktop. Duing system statup, the WIN 98 SE Statup Menu appeas. The menu displays 8 choices fo stating the opeating system including, 8. Pevious vesion of MS-DOS, (XL SYSTEM II softwae) and 1. Nomal, (WIN 98 SE opeating system). If you do not make a selection within 90 seconds, the system automatically stats WIN 98 SE. Micosoft Windows 98 Statup Menu 1. Nomal 2. Logged (\BOOTLOG.TXT) 3. Safe Mode 4. Safe mode with netwok suppot 5. Step-by-step confimation 6. Command pompt only 7. Safe mode command pompt only 8. Pevious vesion of MS-DOS E STARTING XL/XL-MCL SYSTEM II SOFTWARE To stat XL/XL-MCL SYSTEM II softwae, at the WIN 98 SE Statup Menu, pess 8 and then pess Û to select option 8. Pevious vesion of MS-DOS duing system bootup. At the MS-DOS C:\XL> pompt, type XL2 and pess Û. STARTING WIN 98 SE To stat WIN 98 SE, at the WIN 98 SE Statup Menu, pess 1 and then pess Û to select option 1. Nomal. This loads the WIN 98 SE opeating system and displays the WIN 98 SE desktop. Please note that the XL/XL-MCL SYSTEM II softwae is not accessible fom the WIN 5

12 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM THE STARTUP MENU EXITING TO MS-DOS FROM SYSTEM II SOFTWARE 98 SE opeating system. EXPO softwae, if installed, is accessible fom the WIN 98 SE Desktop. EXITING TO MS-DOS FROM SYSTEM II SOFTWARE Select Exit fom the Applications Menu to tun off the SYSTEM II softwae and ente the MS-DOS opeating system. (See the MS-DOS manuals fo moe infomation on the MS-DOS opeating system). The Cytomete emains on. To tun off the Cytomete, type XL OFF and pess Û at the C:\XL> pompt. To etun to WIN 98 SE, eboot the Wokstation and select option 1. Nomal. RESTARTING XL/XL-MCL SYSTEM II SOFTWARE FROM MS-DOS Type XL2 at the C:\XL > pompt and pess Û to etun to the SYSTEM II softwae fom the MS-DOS opeating system. Note: If the Cytomete is off, estating the softwae tuns the Cytomete back on if the estat featue has been pogammed. To pogam this estat featue, go to the SYSTEM II softwae: 1. Select the Utilities Application. 2. Select the Utilities Configuation sceen. 3. Select ON in the Powe field of the Stat Up section of the Utilities Configuation sceen. CYTOMETER COMPUTER MONITOR MCL OPTION Epics XL MCL E 6

13 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON NOTES ON WORKING WITH XL/XL-MCL SYSTEM II SOFTWARE AND WINDOWS 98 SE The XL/XL-MCL SYSTEM II softwae has been validated to opeate in the MS-DOS opeating system only. Theefoe, the softwae must opeate fom the MS-DOS opeating system and it cannot opeate in a Windows 98 SE envionment o an MS-DOS window fom Windows 98 SE. Beckman Coulte can install two XL SYSTEM II softwae icons on the WIN 98 SE desktop of you FlowCente Wokstation which can easily link to the MS-DOS opeating system and launch the SYSTEM II softwae. Use the instuctions in this section if the two XL SYSTEM II softwae icons wee installed on the WIN 98 SE desktop of you FlowCente Wokstation (by you Beckman Coulte Repesentative o peinstalled by Beckman Coulte). Duing system statup, the WIN 98 SE Statup Menu appeas. The menu displays 8 choices fo stating the opeating system including, 8. Pevious vesion of MS-DOS, (XL SYSTEM II softwae) and 1. Nomal, (WIN 98 SE opeating system). If you do not make a selection within 90 seconds, the system automatically stats WIN 98 SE. Micosoft Windows 98 Statup Menu 1. Nomal 2. Logged (\BOOTLOG.TXT) 3. Safe Mode 4. Safe mode with netwok suppot 5. Step-by-step confimation 6. Command pompt only 7. Safe mode command pompt only 8. Pevious vesion of MS-DOS E STARTING WIN 98 SE To stat WIN 98 SE, at the WIN 98 SE Statup Menu, pess 1 and then pess Û to select option 1. Nomal. This loads the WIN 98 SE opeating system and displays the WIN 98 SE desktop. EXPO softwae, if installed, is accessible fom the WIN 98 SE Desktop. STARTING XL/XL-MCL SYSTEM II SOFTWARE To stat the XL SYSTEM II softwae fom the WIN 98 SE desktop, double click. 7

14 STARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON EXITING TO WIN 98 SE DESKTOP FROM SYSTEM II SOFTWARE EXITING TO WIN 98 SE DESKTOP FROM SYSTEM II SOFTWARE Select Exit fom the Applications Menu to tun off the SYSTEM II softwae and estat WIN 98 SE automatically. The Cytomete emains on. To tun off the Cytomete, double click. RESTARTING XL/XL-MCL SYSTEM II SOFTWARE FROM AN ICON Double click the XL SYSTEM II softwae icon fom the WIN 98 SE desktop. to etun to the SYSTEM II softwae Note: If the Cytomete is off, estating the softwae tuns the Cytomete back on if the estat featue has been pogammed. To pogam this estat featue, go to the SYSTEM II softwae: 1. Select the Utilities Application. 2. Select the Utilities Configuation sceen. 3. Select ON in the Powe field of the Stat Up section of the Utilities Configuation sceen. 8

15 SYSTEM COMPONENTS CPU MEMORY Intel Pentium III micopocesso. 128-MB RAM. INPUT DEVICES Two-button wheel mouse 104-key IBM PC-AT compatible WIN 98 SE keyboad Any othe compatible input device (ba-code wand, and so on). DATA STORAGE 3.5-in, 1.44-MB diskette dive 13-GB EIDE had disk 40x CD-ROM dive. OPERATING SYSTEMS MS-DOS 6.22 Windows 98 SE. MONITOR Colo (SVGA) flat panel with built-in speakes. Available choices: t 15-in., maximum esolution of 1024 x 768 pixels t 18-in., maximum esolution of 1280 x 1024 pixels t 21-in., esolution of 1600 x 1200 pixels o highe. INTERFACES Bidiectional asynchonous seial intefaces fo communication with mainfame and pesonal computes Bidiectional Paallel Pot inteface fo connection to pintes SCSI-2 inteface fo connection to intenal o extenal stoage devices Ethenet Inteface fo netwoking to mainfame and pesonal computes 16-bit sound cad inteface to connect speakes. OPTIONAL ACCESSORIES 250-MB intenal Iomega Zip dive 2-GB intenal Iomega Jaz dive Intenal Recodable CD-ROM dive RJ11C inteface via an intenal 56,000 modem to an analog telephone line fo connection to On-Line Intenet Sevices (analog line equied). This can vay by county. 9

16 SYSTEM COMPONENTS OPTIONAL ACCESSORIES 10

17 MAINTAINING SYSTEM PERFORMANCE SCANDISK ScanDisk checks the files and foldes on you had disk fo data eos, and it also checks the physical suface of the disk. ScanDisk can be configued to automatically epai any eos it finds. Refe to the WIN 98 SE help system fo infomation on how to configue ScanDisk. To stat ScanDisk, follow these steps in WIN 98 SE: 1 Select Stat tt Pogams tt Accessoies tt System Tools tt ScanDisk D 2 Follow the instuctions on the sceen A 11

18 MAINTAINING SYSTEM PERFORMANCE DEFRAGMENTING DEFRAGMENTING Use the Disk Defagmente peiodically to defagment files on you had disk. This inceases system pefomance by keeping had disk access time to a minimum. Note: This pocedue may take up to 1 hou o moe to pefom. You can set it up to automatically un at a convenient time, on a egula basis. Please efe to the WIN 98 SE help system fo futhe assistance. To use Defag, follow these steps in WIN 98 SE: 1 Select Stat tt Pogams tt Accessoies tt System Tools tt Disk Defagmente D 2 Follow the instuctions on the sceen A DRIVESPACE: Using DRIVESPACE is NOT RECOMMENDED on the FlowCente Wokstation. MEMMAKER The DOS MemMake pogam optimizes system memoy and fees conventional memoy fo use by applications. If you add any memoy esident pogams o peipheals that use DOS 12

19 MAINTAINING SYSTEM PERFORMANCE ARCHIVING DATA device dives to you system, un MemMake to optimize system memoy. Refe to you DOS manual fo moe infomation and instuctions fo unning MemMake. ARCHIVING DATA Do not use XL SYSTEM II, ALTRA, o Elite softwae to achive data to the optional ecodable CD-ROM dive. Files must be achived to the ecodable CD-ROM dive in WIN 98 SE, using the achiving softwae that came with the dive. CHANGING WIN 98 SE STARTUP MENU CRITERIA Note: Do not pefom this pocedue unless you ae familia with woking with files. You might need to change these settings back to thei oiginal state to toubleshoot poblems. The WIN 98 SE Statup menu is contolled by a file named C:\MSDOS.SYS. Using an editing softwae application such as Notepad fo Windows, you can change the default menu item selection and the time allowed to make the selection. 1 Open the C:\MSDOS.SYS file. 2 Look fo the section of the file stating with [Options] as shown to the ight. 3 Make any of the changes listed below to change the WIN 98 SE Statup menu citeia to meet you needs: Boot Menu = 1. Change the 1 to 0 to suppess the WIN 98 SE Statup menu. Boot Menu Delay = 90. Change the 90 to anothe value to change the numbe of seconds the system waits fo you enty at the WIN 98 SE Statup menu befoe it automatically stats the default selection. Boot Menu Default = 8. Change the 8 to the numbe of the option listed on the WIN 98 SE Statup menu (1-8) that you want to use as the default selection. [Options] Boot Multi = 1 Boot Menu = 1 Boot Menu Delay = 90 Boot Menu Default = 8 13

20 MAINTAINING SYSTEM PERFORMANCE CHANGING WIN 98 SE STARTUP MENU CRITERIA 14

21 SOFTWARE SUPPORT WINDOWS 98 SE GUIDED TOUR At system statup, you FlowCente Multimedia Wokstation uns a guided tou of the WIN 98 SE opeating system. This 10-minute tou teaches you a few basic skills fo using WIN 98 SE. WINDOWS 98 SE HELP SYSTEM WIN 98 SE opeating system contains an extensive help system. The help system can be accessed in WIN 98 SE by selecting Stat and Help. Please efe to the help system fo questions about WIN 98 SE. MICROSOFT TECHNICAL SUPPORT If you have suppot issues o questions egading DOS o WIN 98 SE softwae, contact Micosoft Copoation. Telephone numbes ae listed in a file entitled SUPPORT.TXT, located in the C:\WINDOWS subdiectoy. Micosoft s website also contains answes to most suppot questions, and may be accessed via the intenet at BECKMAN COULTER SOFTWARE TECHNICAL SUPPORT If you have suppot issues o questions egading ALTRA, Elite, EXPO32, EXPO32 ADC, o XL SYSTEM II softwae: In the USA contact Beckman Coulte Technical Sevices at Outside the USA, contact you local Beckman Coulte Repesentative. 15

22 SOFTWARE SUPPORT BECKMAN COULTER SOFTWARE TECHNICAL SUPPORT 16

23 TRADEMARKS The BECKMAN COULTER logo, ALTRA, COULTER, Elite, EPICS, FlowCente, SYSTEM II, XL, and XL-MCL ae tademaks of Beckman Coulte, Inc. All othe tademaks, sevice maks, poducts, o sevices ae tademaks o egisteed tademaks of thei espective holdes.

24 Come visit us at Pinted on Recycled Pape Copyight Beckman Coulte, Inc , 2010 All Rights Reseved

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