Skin & Bio Research

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1 Skin & Bio Research

2 Scientific Beauty Substantiation Global leader in Asian cosmetics testing Ellead is a clinical and biotechnology company which is specialized in cosmetics and food supplement. Since 2001, Ellead has been performing clinical studies according to protocols for efficacy, safety, sensory tests of cosmetics, cosmeceuticals, and functional food products with great accuracy and reliability. Good Laboratory Pratice (GLP) certified by Korea Ministry of Food and Drug Safety ISO 9001: 2008 certification IRB (Institutional Review Board) registration

3 History Certified Good Laboratory Practice (GLP) by MFDS - Received Best Work Environment Award - Establishment of in vitro research center at Osong, Korea - Development of improved evaluation method of UVA sunscreen (MFDS Cosmetic Efficacy Evaluation Guideline) - Validation of in vitro UVA evaluation method (MFDS) - Presentation on Functional Cosmetics in Korea at One Asia Beauty Forum in China Venture Industry Activation Merit Award (received MOTIE Minister Award) - Developed evaluation method for irritation test using artificial skin - Development of evaluation method for edema improvement and skin blood flow (MFDS Cosmetic Efficacy Evaluation Guideline) - Registered IRB with Korea Centers for Disease Control and Prevention - Development of evaluation method for dark circle alleviation (KFDA Cosmetic Efficacy Evaluation Guideline) - Development of evaluation method for proper acne skin care (KFDA Cosmetic Efficacy Evaluation Guideline) - Changed CI and opened second R&D Center - Registered IRB with US Food and Drug Administration - Received Million Dollar Export Award - Field trip of cosmetic officialdom of China, Thailand, Philippines and Indonesia - Certified as technically innovative (INNO-BIZ) company - Development of evaluation method for water-resistant sunscreen of SPF (KFDA Cosmetic Efficacy Evaluation Guideline) - Certified as venture company - Acquired ISO 9001: Technology exchange with DermExpert - Certified as the industry s first R&D center by Korean Industrial Technology Promotion Association - Established Ellead

4 Core Values Trust Sophistication Our dermatologists have various clinical experiences and our skilled researchers provide the most suitable testing method to meet the demands of our customers. We perform clinical trials in accordance with Good Clinical Practice (GCP) under the supervision of our dermatologists. Advance In order to correspond with the current rapidly changing market, Ellead strives to extend its business spectrum to provide total clinical evaluation service focused on customer satisfaction by building a network with research and development centers and universities. We continue to develop new instruments, software and studies to maintain our advanced technologies. Reliance Ellead has been acknowledged for producing reliable and accurate results and accredited by the government. Many research results were presented at academic conferences. Now multinational corporations trust our research results and maintain a business partner. Ellead Skin & Bio Research

5 Efficacy / Safety / Sensory Evaluation Quality Management Standard Operating Procedures (SOP) set to meet various international regulations Studies conducted based on GCP guideline Ellead Institutional Review Board (IRB) registered with Korea and US FDA Quality Assurance system for high reproducibility and accuracy State of the art GLP compliant facilities Dermatologist-controlled studies Patented scientific photography system Multiple instruments to ensure reliability Various protocols tailored to each customer s needs CL INIC ICAL EVA VALU LUAT ATIO ION

6 Testing Overview Testing for - Cosmetic products - Active ingredients - Raw materials - Hair care products - Toiletries - Household products - Functional foods - Cosmetic devices Service fields In vitro test for cosmetic ingredients and products - Cytotoxicity test - Efficacy evaluation In vitro efficacy test Clinical test Clinical test for Efficacy evaluation - Functional cosmetics - Functional foods - Quasi-drugs Safety test Clinical test for Safety evaluation - Primary patch test - Repeated insult patch test - Cumulative irritation test Ellead Skin & Bio Research

7 Efficacy / Safety / Sensory Evaluation Clinical safety test Patch test Safety testing of cosmetic ingredients and finished products is of utmost importance. A variety of patch test designs are available to test for irritation or sensitization in the product development process. Primary patch test Repeated insult patch test (RIPT) Cumulative irritation test (CIT) Comedogenicity test Comedogenic potential is tested by counting the number of comedones. Follicular biopsy test on the back Home use test with visual assessment In-use test In-use test is designed to test your product on the consumer s view and dermatologist examination. Self-assessment questionnaires (satisfaction & tolerance) Adverse reaction Eye irritation test Eye irritation test is conducted in collaboration with ophthalmologist to study the level of sting and irritation in the eye that occur from contacting a product. CLINICAL EVALUATION

8 Clinical efficacy test Skin care Clinical efficacy tests are conducted to test and substantiate the product s intended purposes. We offer a wide range of tailored studies to claim your product s efficacy and to maximise its impact in the marketplace using the latest equipment and technology. Anti-aging Anti-wrinkle* Skin whitening* Sunscreen (SPF, PA, Waterproof, Sweatproof)* Skin hydration & lasting effect Trans-epidermal water loss (TEWL) Skin barrier recovery Skin elasticity Dermal density Facial lifting Skin sebum Blackhead Cleansing (Pore, Make-up) Skin desquamation Turn over Skin gloss Translucency Dark circle Cellulite Puffiness Stretch mark Skin temperature Microcirculation Anti-microbial (Handwash) Various test areas Whitening Anti-wrinkle Before After In Korea, anti-wrinkle, whitening and sunscreen products are categorized as functional cosmetics Ellead Skin & Bio Research

9 Efficacy / Safety / Sensory Evaluation Clinical efficacy test Cheek sagging Skin temperature Before After Facial lifting Dermal density Sunscreen We have many Multiport Solar Simulators dedicated to each SPF, PA and water resistance test to offer multiple product testing according to the desired standard listed below. We also offer in vitro SPF and PA test according to ISO and US FDA standards. Available standards: SPF PA Water resistance In vitro MFDS MFDS MFDS water resistance ISO ISO ISO FDA final rule (2011) FDA final rule (2011) FDA final rule (2011) JCIA Colipa water resistance Cosmetics Europe CFDA CFDA CFDA CLINICAL EVALUATION

10 Clinical efficacy test Make-up A variety of makeup products from foundations to eye makeup products can be tested to prove their efficacies on improving skin tone and lightening as well as for long lasting coverage, waterproof, curling and volumizing effect. Makeup color lasting effect Homogeneity Makeup coverage & lasting effect Mascara & eyeliner waterproof effect Eyelash curling & volume Nail color lasting effect Color durability and homogeneity Eyelash curling Before After Overlay Lip care Lip care products are applied topically to help moisturize and relieve skin dryness and chapping, and are tested for such claims. Hydration Desquamation Roughness Hydration of lips Before After Ellead Skin & Bio Research

11 Efficacy / Safety / Sensory Evaluation Clinical efficacy test Hair care A wide range of hair product testing is available including hair growth studies with phototrichogram as well as hair condition, color, strength and style testing services. Hair growth Hair damage Gloss Hydration Tensile strength Elasticity Curling Volume Dye color durability Scalp sebum Desquamation Scalp hydration Elasticity Hair gloss Hair growth Hair volume Before After Hair damage care Scalp desquamation Before After Before After Before After CLINICAL EVALUATION

12 Clinical efficacy test Quasi-drugs Quasi-drugs are the products that have minimal to moderate pharmacologic (cosmeceutical) activity. The efficacy tests for quasi-drugs are conducted according to available guidelines. Hair loss Acne Atopic dermatitis Anti-microbial (Sanitizer) Deodorant Dandruff Acne study Before After Intimate hygiene Intimate hygiene products are tested in collaboration with gynecologist and dermatologist. Vaginal health grading ph measurement Skin hydration Self-assessment Oral care Oral care products, such as tooth paste or mouthwash, can be tested for tooth whitening effect, oral hygiene and breath odor. Tooth whitening Oral hygiene Breath odor Ellead Skin & Bio Research

13 Efficacy / Safety / Sensory Evaluation Functional foods Skin care Efficacy claims such as anti-wrinkle, whitening and hydration effects and safety of food supplements are tested after the approval of Ellead Institutional Review Board. Visual assessment Skin hydration Wrinkle Elasticity Blood examination Urine examination GLP study (certified by MFDS) Skin irritation test Ellead has acquired Good Laboratory Practice (GLP) certification by Korea Ministry of Food and Drug Safety (MFDS) for in vitro Skin Irritation: Reconstructed Human Epidermis Test Method (OECD TG439). CLINICAL EVALUATION

14 In vitro test In vitro test in vitro testing is essential in raw material / active ingredient development. Ellead offers various efficacy and safety tests customized to your needs at our new Ellead Osong research center dedicated solely for in vitro testing. Cytotoxicity & cell viability MTT assay WST-1 assay Alamar blue assay Anti-oxidant DPPH radical scavenging assay ORAC (Oxygen radical absorbance capacity) assay Total polyphenol quantification assay ROS (Reactive oxygen species) scavenging assay Anti-wrinkle Collagen synthesis assay Collagenase inhibition assay (MMP-1, MMP-3) Elasticity Elastase inhibition assay Anti-inflammation Nitric oxide inhibition assay Cytokine inhibition assay (IL-6, TNF-α) Protein inhibition assay (COX-2, PGE 2 ) Moisture & Skin barrier mrna expression analysis (Aquaporin, Hyaluronan synthase, Involucrin, Filaggrin) Protein expression analysis (Involucrin, Filaggrin) Alternatives to animal testing in vitro Skin Irritation : Reconstructed Human Epidermis Test Skin absorption test using diffusion cell in vitro 3T3 NRU phototoxicity test Whitening in vitro tyrosinase inhibition assay in vitro DOPA oxidation inhibition assay Melanogenesis inhibition assay Others mrna expression analysis by RT-PCR, RT-qPCR Protein expression analysis by Western blot Ellead Skin & Bio Research

15 CLINICAL EVALUATION

16 Sophistication Advance Reliance ISO 9001:2008 Certified GLP (Good Laboratory Practice) Certified IRB (Institutional Review Board) Registered Ellead Co., Ltd. [HQ] 325, Hwangsaeul-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea Tel: Fax: [BRANCH] Venture center II, , Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, Republic of Korea Tel: Fax:

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