PCR Enzyme Selection Guide

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1 PCR Enzyme Selection Guide T: 1 (800) , W: E: order@biobasic.com, Copyright Bio Basic Inc. All rights reserved.

2 Contents 1. PCR Enzymes Overview and Comparison. 2. Featured Product 1: High Reverse Transcriptase Kit (RT PCR). 3. Featured Product 2: High Taq DNA with 10mM dntp Mix. T: 1 (800) , W: E: order@biobasic.com, Copyright Bio Basic Inc. All rights reserved.

3 1. PCR Enzymes Overview and Comparison (page 1 of 2). Specifica ons Bio Basic Product Name Cat. # Product Comparison General PCR Routine Performance Tsg DAN D0081 Regular PCR Taq DNA B0089(D0089) Taq DNA with dntp Mix 9K Taq PCR Master Mix (2X, Blue Dye) BS9296 Premix with Dye Taq PCR Master Mix (2X, Red Dye) BS9298 Host DNA free Taq DNA (High Purity) HTD0078 pfu DNA B0093(D0090P) Proof Reading Taq Plus DNA D0090 Pfu DNA polymerase with dntp mix 9K Fast Taq DNA polymerase with dntp mix 9K Genotyping Wise Taq Premix (96 Rx) 9K [Ampliqon] Taq DNA [Bioneer] AccuPower Taq [Bioron] Top TaqDNA [Elpis] rtaq DNA Polyemerase, HiPi DNA Polyemerase, HiPi Eco DNA Polyemerase [Enzynomics] ntaq [Epicentre] MasterAmp DNA [Intron] i Taq DNA, i Taq plus DNA [KAPA] KAPA Taq PCR Kit [Life technologies/ Invitrogen] Taq DNA, SuperTaq, AmpliTaq DNA [NEB] Taq DNA, Crimson Taq DNA [Promega] GoTaq DNA, GoTaq Flexi DNA [Qiagen] Taq DNA, TopTaq DNA [Roche] Taq DNA [Solis biodyne] FIREPol DNA [TaKaRa] TaKaRa Taq [Thermo scientific / Fermentas] Taq DNA, DreamTaq DNA [Toyobo] rtaq DNA Long Range PCR Long Targets LR DNA with 10mM dntp Mix 9K [Bioneer] AccuPower ProFiTaq [Elpis] HiPiSuper DNA [Enzynomics] ntaq Tenuto [Epicentre] MasterAmp Extra Long PCR Kit [Intron] i MAXTMII DNA [KAPA] KAPA Long Range DNA [Life technologies/ Invitrogen] SuperTaq Plus [NEB]LongAmp Taq DNA, Crimson Long Amp Taq DNA [Qiagen] QIAGEN Long Range PCR Kit [Roche] Expand 20 kb PLUS PCR System, Expand Long Template PCR System [TaKaRa] TaKaRaLA Taq,TaKaRaEx Taq [Thermo scientific / Fermentas] Long PCR Enzyme Mix [Toyobo]Blend Taq Hot Start PCR Chemical Mediated Genotyping 3G HotStart Taq DNA High Taq DNA with 10mM dntp Mix 3GHST81 9K [Ampliqon] TEMPase Hot Start DNA [Enzynomics] ntaq HOT [Intron] i StartTaq DNA [Life technologies/ Invitrogen] AmpliTaqGold DNA [Qiagen] Hot Start Taq DNA [Roche] Fast Start Taq DNA [Solis biodyne] HOT FIRE Pol DNA [Thermo scientific / Fermentas] Maxima Hot Start TaqDNA, TrueStart Hot Start Taq DNA

4 1. PCR Enzymes Overview and Comparison (page 2 of 2). Specifica ons Bio Basic Product Name Cat. # Product Comparison Multiplex PCR Routine Performance 2X Multiplex PCR Premix 9K [Bioneer] AccuPower Gold Multiplex PCR PreMix, AccuPower Multiplex PCR PreMix [Enzynomics] ntaq multi HOT [KAPA] KAPA 2G Fast Multiplex Kit [Life technologies/ Invitrogen] Platinum Multiplex PCR Master Mix [NEB]Multiplex PCR 5X Master Mix [Qiagen] QIAG qpcr RT PCR qrt PCR Real Time PCR (quantitative PCR) Reverse Transcriptase End point PCR (conventional PCR) Reverse Transcriptase Real Time PCR (qpcr) Green 2 Go qpcr Mastermix ROX (500X20ul Rxn) Green 2 Go qpcr Mastermix Low ROX (500X20ul Rxn) Green 2 Go qpcr Mastermix icycler (500X20ul Rxn) Green 2 Go qpcr Mastermix (500X20ul Rxn) High Reverse Transcriptase (RNase H ) One Step RT PCR Kit 5X All In One RT MasterMix One Step M MULV RT PCR Kit One Step AMV RT PCR Kit 5X All In One RT MasterMix (100 x 10 µl Rxns) 5X All In One RT MasterMix (200 x 10 µl Rxns) 5X All In One RT MasterMix (with Genomic DNA Removal) 5X All In One RT MasterMix (with Cell Lysis) One Step MultiPlex RT qpcr Kit with Probe Green 2 Go 1 Step RT qpcr Mastermix No Dye Green 2 Go 1 Step TaqProbe RT qpcr Mastermix no Dye Green 2 Go 1 Step RT qpcr Mastermix Rox Green 2 Go 1 Step RT qpcr Mastermix icycler Green 2 Go 1 Step RT qpcr Mastermix Low Rox QPCR001 R QPCR002 L QPCR003 ic QPCR004 S 9k K HRT BS665 BS6649 HRT HRT HRT100 20G HRT100 20L 9K QPCR007 NODYE QPCR008 NODYE QPCR010 R QPCR011 ic QPCR012 LR [Thermo scientific] Maxima Probe/ROX qpcr Master Mix (2X) [Thermo scientific] Luminaris Color HiGreen qpcr Master Mix, low ROX [Promega] GoTaq qpcr Master Mix [Thermo scientific] SYBR GreenER qpcr SuperMix for icycler Instrument [Thermo scientific] SYBR Green Real Time PCR Master Mix [Qiagen] QIAGEN OneStep RT PCR Kit [Invitrogen] SuperScript III One Step RT PCR System with Platinum Taq [NEB] OneTaq One Step RT PCR Kit [Promega] Access RT PCR System [Roche] TITAN ONE TUBE RT PCR SYSTEM [Invitrogen] SuperScript III Platinum One Step qrt PCR Kit

5 2. Featured Product 1: High Reverse Transcriptase Kit (RT-PCR) Catalog #: Size: 9K ,000U Kit Contents: High RTase (200 U/ul). 5X High RT Reac on Buffer (includes RNAse Inhibitor, 50mM DTT and dntp). Features: cdna synthesis with high processivity and sensi vity. Op mal ac vity at 50 C (42 55 C)for great efficiency. Synthesis of long length cdna (<14kb) from RNA. Fast and easy one step RT PCR reac on in a single tube. Description: The High Reverse Transcriptase Kit is a complete system for synthesis of cdna from RNA which is using RTase, a recombinant type of reverse transcriptase from M MLV. The exis ng wild type of reverse transcriptase may have lower efficiency of cdna synthesis due to its ac va on temperature at 42 C or below, however this mutant form has greater efficiency as inhibi on of RNA s secondary structure by its thermostable characteris c at 50 C for ac va on. Therefore, this kit is a successful system in synthesis of 1st stranded cdna up to 14kb from total RNA. The High Reverse Transcriptase Kit is composed of RTase, Buffer (including dntp, DTT and 2X RT Pre mix) is supplied as an easy to use mastermix which includes an op mized rate of RTase, RNase inhibitor, DTT and dntp in 0.2ml 8 strip tubes. CHARACTERISTICS Reverse transcriptase M MLV(Recombinant) Optimal reaction temperature 50 C Sensitivity 10 pg 1 μg Total RNA RNase H activity RNaseH Max cdna length < 14 kb Random, Oligo d(t), Applicable Primers Gene specific primer APPLICATION Reverse transcription cdnalibrary construction Multiplex PCR Molecular diagnosis Comparison of amplification efficiency for various RNA concentrations: Right: Reverse transcrip on (RT) Comparison result between Bio Basic s High Reverse Transcriptase Kit and compe tors for amplifica on efficiency. High Reverse Transcriptase Kit The High Reverse Transcriptase Kit also provided high sensi vity, specificity and stability for cdna synthesis against the GC rich template. C

6 3. Featured Product 2: High-Taq DNA with 10mM dntp Mix Catalog #s: Size: 9K ; 9K U (9K ); 1000U (9K ) Kit Contents: High Taq DNA (2.5U/ul). 10X High Taq Reac on Buffer. 10mM dntp Mix (10mM each). Band Sharpener. Description: Features: Hot Start PCR system (chemical mediated PCR enzyme). High specificity. Reducing non specific primer binding and amplifica on for high specificity. Applicable to mul plex PCR/ allele specific PCR. Bio Basic s High Taq DNA is a chemical mediated Hot Start Taq DNA polymerase, which is thermostable DNA polymerase from thermus aqua cus. This recombinant Taq DNA polymerase is inac ve at room temperature avoiding extension of non specifically annealed primers or primer dimers and providing higher specificity of DNA amplifica on, and its func onal ac vity of the enzyme is restored during 15 minute incuba on at 95 C. High Taq DNA is suitable for mul plex PCR or allele specific PCR in diagnos cs or screening which is required high specificity and high sensi vity in PCR due to its characteris cs. It enables amplifica on of target DNA up to 21 in a single reac on at once. Op mal buffer system and op onal 5X Band Sharpener helps amplifica on of difficult PCR which is due to high GC content and complex structure. CHARACTERISTICS 5 to 3 Exonuclease activity Yes 3 to 5 Exonuclease activity (Fidelity) No Fidelity vs. Taq 1x 3 A or Blunt ends 3 A Target length genomic/phage DNA < 2.0 kb Hot start activity Yes (Chemical mediated) Enzyme activation time (95 C) 15 min Extension times 1 min /kb Host DNA free No APPLICATION Colony PCR General sequencing Genotyping Allele Specific PCR Multiplex PCR TA Cloning Molecular diagnosis Rice Allele specific PCR Application: Right: High Taq DNA based 2X mul plex detec on with 18 SNP marker, according to NAQS (Na onal Agricultural Product Quality Management Service).

7 T: 1 (800) , W: E: order@biobasic.com, Copyright Bio Basic Inc. All rights reserved.

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3

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