BD IMag TM Cell Separation System
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1 BD IMag TM Cell Separation System
2 BD IMag TM Cell Separation Sytem BD IMag TM Cell Separation System BD IMagnet TM direct magnet technology BD IMag TM Buffer Buffer 5 BD IMagnet TM 6 BD Falcon TM (IMag Particles IMag Particles IMagnet 4 5 Negative Positive 1
3 IMagnet IMagnet Human Cat. No. Description Clone Size Anti-Human CD3 Particles - DM HIT3a 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD4 Particles - DM L mL ( 1 x 10 9 cells ) 78, Anti-Human CD8 Particles - DM SK1 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD11c Particles - DM S-HCL-3 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD14 Particles - DM M P9 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD19 Particles - DM HIB19 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD19 Particles - DM 4G7 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD25 Particles - DM 2A3 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD45RA Particles - DM HI mL ( 1 x 10 9 cells ) 78, Anti-Human CD45RO Particles - DM UCHL1 5.0mL ( 1 x 10 9 cells ) 78, Anti-Human CD56 Particles - DM NCAM mL ( 1 x 10 9 cells ) 78,000 Mouse Cat. No. Description Clone Size Anti-Mouse CD4 Particles - DM GK mL ( 2 x 10 9 cells ) 51, Anti-Mouse CD8a Particles - DM mL ( 2 x 10 9 cells ) 51, Anti-Mouse CD11b Particles - DM M1/ mL ( 2 x 10 9 cells ) 51, Anti-Mouse CD45R/B220 Particles - DM RA3-6B2 10.0mL ( 2 x 10 9 cells ) 51, Anti-Mouse CD90.2 (Thy-1.2) Particles - DM 30-H mL ( 2 x 10 9 cells ) 51, Anti-Mouse Ly-6G and Ly-6C (Gr-1) Particles - DM RB6-8C5 10.0mL ( 2 x 10 9 cells ) 51, Anti-Mouse TER-119 Particles - DM TER mL ( 1 x 10 9 cells ) 51,000 Rat Cat. No. Description Clone Size Anti-Rat CD4 Particles - DM OX mL ( 1 x 10 9 cells ) 78,000 Mouse Cat. No. Description Size Mouse NK Cell Separation Set - DM 10.0mL ( 2 x 10 9 cells ) 60,000 PE Rat Anti-Mouse CD49b Anti-PE Particles 2 - DM Human T Cell Receptor Separation Set - DM 5.0mL (1 x 10 9 cells ) 78,000 Biotin Mouse anti-human TCR Streptavidin Particles Plus 3 -DM 2
4 Biotin IMagnet IMagnet IMagnet Human Cat. No. Description Size Human T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD11b/Mac-1 (CR3), CD16, CD19, CD36, CD41a, CD56, CD235a (Glycophorin A) Human CD4 T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD8, CD11b/Mac-1 (CR3), CD16, CD19, CD36, CD41a, CD56, CD123, CD235a (Glycophorin A), TCR Human Naïve CD4 + T Cell Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 98,000 CD8, CD11b, CD16, CD19, CD36, CD41a, CD45RO, CD56, CD123 TCR, CD235a (Glycophorin A) Human CD8 T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD4, CD11b/Mac-1 (CR3), CD16, CD19, CD36, CD41a, CD56, CD123, CD235a (Glycophorin A), TCR Human Naive CD8 + T Cell Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 98,000 CD4, CD11b, CD16, CD19, CD41a, CD45RO, TCR CD235 (Glycophorin A) Human Memory CD4 + T Cell Enrichmentt Set - DM 5.0mL ( 1 x 10 9 cells ) 110,000 CD8, CD11b, CD16, CD19, CD36, CD41a, CD45RA, CD56, CD123, CD235a (Glycophorin A), TCR Human B Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD3, CD41a, CD43, CD235a (Glycophorin A) Human Dendritic Cell Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 135,000 CD3, CD14, CD19, CD41a, CD56, CD66b, CD235a (Glycophorin A), IgE Human Monocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 98,000 CD3, CD45RA, CD19, CD56, CD235a (Glycophorin A), CD41a* Human NK Cell Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD3, CD19, CD36, CD41a, CD66b, CD123, CD235a (Glycophorin A), IgE Human Lineage Cell Depletion Set - DM 5.0mL ( 1 x 10 9 cells ) 98,000 CD3, CD14, CD16, CD19, CD41a, CD56, CD235a (Glycophorin A) Cat# Human Monocyte Enrichment Set - DM CD41a 1 3
5 Mouse Cat. No. Description Size Mouse T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD11b, CD45R/B220, CD49b, TER Mouse CD4 T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD8a, CD11b, CD45R/B220, CD49b, TER Mouse CD8 T Lymphocyte Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD4, CD11b, CD45R/B220, CD49b, TER Mouse B Lymphocyte Enrichment Set- DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD4, CD43, TER Mouse Hematopoietic Progenitor (Stem) Cell Enrichment Set - DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD3e, CD11b, CD45R/B220, Ly-6G and Ly6C,TER Mouse NK Cell Enrichment Set-DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD4, CD5, CD8a, CD19, CD24, Ly-6G and Ly6C, TER Mouse Dendritic Cell Enrichment Set- DM 5.0mL ( 1 x 10 9 cells ) 80,000 CD2, CD3e, CD45R/B220, CD49b, CD147, LY-6G and Ly6C TER-119 BD IMag Purity (% of final enriched fraction) Recovery (% of original cell population) B cells T cells CD4 T cells CD8 T cells NK cells 0 B cells T cells CD4 T cells CD8 T cells NK cells 1 2 BD IMag Purity (% of final enriched fraction) B cells T cells CD4 T cells CD8 T cells NK cells Hematopoietic Progenitor cells Dendritic Cells* Recovery (% of original cell population) B cells T cells CD4 T cells CD8 T cells NK cells Hematopoietic Dendritic Progenitor cells Cells* 3 4 BD IMag 4
6 IMagnet Negative Positive Negative Positive APCAPC Human Cat. No. Description Size Human Regulatory T Lymphocyte Separation Set - DM 5.0mL ( 1 x 10 9 cells ) 135,000 CD8, CD11b/Mac-1 (CR3), CD16, CD19, CD36, CD41a, CD56 CD123, CD235a (Glycophorin A), TCR APC CD25 Cat. No. Description Size Streptavidin Particles Plus - DM 5.0mL ( 1 x 10 9 cells ) 39, Anti-R-Phycoerythrin (PE) Particles - DM E mL ( 1 x 10 9 cells ) 72, Anti-Allophycocyanin (APC) Particles - DM E mL ( 1 x 10 9 cells ) 72, Anti-Mouse IgG1 Particles - DM A mL ( 1 x 10 9 cells ) 72, Anti-Rat Ig, Light Chain Particles - DM MRK-1 5.0mL ( 1 x 10 9 cells ) 72,000 BD IMag TM Buffer Cat. No. Description Size BD IMag Buffer (10X) 100mL 8,000 5
7 BD IMagnet TM Neodymium-Iron-Boron6,000 BD Falcon 12 75mm 5mL 6 17x100mm 14mL2 Cat. No. Description BD IMagnet Cell Separation Magnet 95,000 BD IMag TM Cell Separation System BD Falcon TM Cat. No. Description Size mL 12mm x 75mm 1,000 31, mL 12mm x 75mm 1,000 45, mL 12mm x 75mm , mL 17mm x 100mm 1,000 40, mL 17mm x 100mm 1,000 58, mL 17mm x 100mm ,200 6
8 BD IMag TM Cell Separation System Q&A Q IMag Particles - DM A BD IMagnet TM IMag Particles - DM Q BD IMag A nm Q BD IMag A 612x75mm x 10 6 cells/ 1200 x 10 6 cells total 217x100mm x 10 6 cells/ 1200 x 10 6 cells total Q BD IMag A BD Falcon TM R
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