1 ACTA SCIEN TIAE CIRCUMSTAN TIAE Vol. 18,No. 5 Sept, ,2 1 (1., , ), (UV2B, nm). UV2B,, SOD,MDA, mw m mw m - 2, 120 mw m - 2,. MDA SOD,. UV2B ; ; ; ;. 1,. 200, [1 ].,,,, [1 ]., UV2B [1 ]. UV2B, UV2B, UV2B ( T riticum aestiv um ) ,,,. 4 12,4 23, UV2B, 10 :00 17 :00 7 h ( ), 7 21.,. 30W UV2B ( nm),, ( ), 297 nm. 0 ( ) mw m - 2 4, 0 % 12 % 20 % 25 % O 3, 2 m : Arnon (1949) [2 ]. SOD : (1983) [3 ]. 3 ( , ).
2 5 : 505 SOD NB T, NB T 50 %. (MDA) : (1984) [4 ]. : Murali Teramu2 ra (1985) [5 ], 305 nm. : Li21600 ( ),10 :00 17 :00 4, 10,. :, 20. :. : W 222IS. : N N, = N 517. :,HITACHI UV2B UV2B,. 60 mw m - 2 UV2B,, 5 %. 100 mw m - 2 UV2B 20 % 5 cm.,,. 120 mw m - 2 UV2B,50 % 20 %, 15 cm,,,,., UV2B,. 312 UV2B UV2B, a b (a + b), ( 1). 120 mw m - 2 UV2B, a b (a + b) % % %, % %, b UV2B,,, UV2B. 1 UV2B ( mg g( FW) - 1 ) Table 1 The effect of UV2B radiation on the content of chlorophyll in leaves of wheat (mg g( FW) - 1 ) UV2B, mw m - 2 a b (a + b) a b (a + b) P < 0101, ; < P < 0105, 313 UV2B SOD MDA SOD MDA. 1, UV2B, SOD, ( r = ),,MDA, ( r = ). UV2B 100 mw m - 2,SOD MDA, UV2B mw m - 2,SOD, MDA,.,
3 UV2B, UV2B. 2,UV2B,, ,. UV2B mw m 22,, UV2B, UV2B. 1 UV2B SOD MDA a1sod b. MDA Fig. 1 The effect of UV2B radiation on SOD activity and the content of MDA in leaves of wheat 2 UV2B a. b. Fig. 2 The effect of UV2B radiation on the content of flavonoid in leaves of wheat 3 UV2B a1 b1 Fig. 3 The effect of UV2B radiation on stomatal conductance and transpiration in leaves of wheat 315 UV2B. UV2B, ( 3), mw m 22, ,,, UV2B. 316 UV2B UV2B,, ( 2). 120 mw m - 2 UV2B, % % %, UV2B.,
4 5 : 507 ( 2), 0125 % 616 %,,. 10 % 20 %,RAF 116, % %,,, UV2B CO 2 H 2 O P,. 2 UV2B Table 2 The effect of UV2B radiation on the yield of wheat UV2B,mW m , %,g y = x ,g y = x , y = x , P < UV2B 3, 3 UV2B ( %). 60 mw m mw m - 2, Table 3 The effect of UV2B radiation on the quality in seed of wheat ( %), 120 mw m - 2, 6111 %. UV2B UV2B UV2B,mW m mw m mw m - 2 UV2B, 5179 % 8136 %, 120 mw m - 2, 6179 %. 15 ( 4),,, 100 mw m - 2, 90 % 1214 % % %, 120 mw m - 2, 40 % 25 % % %. 15,. 4 UV2B. UV2B SOD MDA, ( r ).,MDA (a + b) ,. SOD, O - 2,, MDA, [6 ],. Wright (1981) [7 ] UV2B K + 2A TPase,.,. Teramura (1983) [1 ],UV2B. UV2B [8,9 ], [9 ],
5 UV2B ( g 100g - 1 ) Table 4 The effect of UV2B radiation onthe content of amino acid in seed of wheat (g 100g - 1 ) UV2B (mw m - 2 ) [10 ], [1 ] [10 ]. UV2B K + 2A TP ase [7 ], K + [8,11 ]. Teramura ( 1990 ) [12 ] Ben2 nett [13 ] UV2B., UV2B, [12 ]. UV2B. UV2B [5 ], DNA [14 ], UV2B [15 ], [11 ]., UV2B,,.,UV2B CO 2 P,.,. UV2B,,UV2B. 1 Teramura A H. Effect of ultraviolet2b radiation on the growth and yield of crop plants. Physiol Plant, 1983, 58 : Arnon D I. Copper enzymes in isolated chloroplasts poly2phenoloxidase in Bete v ulgaris. Plant Physiol, 1949,24 :1 4 3,, 1 1,1993,9 (1) : ,, 1 1,1984,26 (2) : Murali N S, Teramure A H. Effects of ultraviolet2b irradiance on soybean. VI. Influence of phosphorus nutrition on growth and flavonoid content. Physiol Plant, 1985,63 : Sakaki T, Kondo N, Sugahara K. Breakdown of photosynthetic pigments and lipid in spinach leaves with ozone fumigation : Role of active oxygen. Physiol Plant, 1983,59 : Wright L A, Murphy T M, Travis R L. The effect of ultraviolet radiation on wheat root vesicles enriched in plasma mem2 brane. Photochemistry and Photobioloty, 1981,33 : Negash L, Bj orn L O. Stomatal closure by ultraviolet radiation. Physiol Plant, 1986,66 : Murali N S, Teramura A H. Intraspecific differences in Cucumis sativ us sensitivity to UV2B radiation. Physiol Plant, 1986, 68 : Mirecki R M, Teramura A H. Effect of ultraviolet2b irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol, 1984,74 : Wright L A, Murphy T M. Short2wave ultraviolet light closes leaf stomata. Am J Bot, 1982,69 : Teramura A H, Sullivan J H, Ziska L H. Interaction of elevated ultraviolet2b radiation and CO 2 on productivity and photosyn2 thetic characteristics in wheat, rice and soybean. Plant Physiol :
6 5 : Bennett J H. Photosynthesis and gas diffusion in leaves of selected crop plants exposed to ultraviolet2b radiation. J Environ Qual, 1981,10 : Beggs C J, Andrea S J, Eckard W. Isoflavonoid formation as an indication of UV stress in bean ( Phaseolus v ulgaris) leaves. Plant Physiol, 1985,79 : Sullivan J H, Teramura A H. The effects of ultraviolet radiation on loblolly pine. I. Growth, photosynthesis and pigment pro2 duction in greenhouse2grown seedling. Physiol Plant, 1989,77 : THE EFFECT OF ENHANCED UV2B RADIATION ON THE PHYSIO2 LOGICAL INDICATOR, YIELD AND QUAL ITY OF WHEAT Li Yuan 1,2, Wang Xunling 1 (1. Department of Biology, Lanzhou University, Lanzhou Department of Environmental Science, Yunnan Agricultural University, Kunming ) ABSTRACT Influences of enhanced ultraviolet radiation ( UV2B, nm) on physiological indi2 cator, yield and quality of wheat plants grown in filed were investigated. With the increase in UV2B radiation, leaf visible injury became serious, chlorophyll content, SOD activity, stomatal conductance, transpiration in leaves, biomass, ear seed number and ear seed weight decreased obviously, MDA con2 tent, flavonoid content and all sugar content in seed increased, rough protein content, total amino acid content and 15 amino acid content decreased with treatment of 60 mw m - 2 and 100 mw m - 2 UV2B ra2 diation, and they were higher than control with treatment of 120 mw m - 2, rough starch content in seed had no obvious changes. MDA content had significant negative correlation with the chlorophyll content and SOD activity, it suggested that the metabolic disorder and various injuries in leaves induced by UV2B radiation were the result of the increase of membrane lipid peroxidation. Keywords UV2B radiation, wheat, physiological indicator, yield, quality