Inhibitory effects of Psidium guajava and Atractylodes lancea on aquatic pathogenic bacteria

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Abigayle Sharp
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1 South China Fisheries Science Vol. 9 No. 5 Oct. 203 doi: / j. isssn ( ; ) : ( Vibrio coralliilyticus) ( V. harveyi) ( Photobacterium damselae subsp piscicida) ( V. parahaemolyticus) ( Morganella fulton) ( V. vulnificus) 6 7 ( Atractylodes lancea) ( Psidium guajava) ( ) ( ) 0-3 gml gml - > > > > > : ; ; : S 966 : A : ( 203) Inhibitory effects of Psidium guajava and Atractylodes lancea on aquatic pathogenic bacteria WANG Ruixuan FANG Weibin GUO Zhixun WANG Jiangyong (. Key Lab. of South China Sea Fishery Resources Exploitation & Utilization Ministry of Agriculture; Key Lab. of Fishery Ecology Environment Guangdong Province; South China Sea Fisheries Research Institute China Academy of Fishery Sciences Guangzhou China; 2. Guangdong Pharmaceutical University Guangzhou China) Abstract: Through microdilution method we studied the inhibitory effects of different processed products of Atractylodes lancea and water extract from different parts of Psidium guajava on 7 strains from6 bacterial species including Vibrio coralliilyticus V. harveyi Photobacterium damselae subsp piscicida V. parahaemolyticus Morganella fulton V. vulnificus. The minimal inhibitory concentration ( ) and minimal bactericidal concentration ( ) for different strains were determined. Results show that different Chinese medicine processed products of Atractylodes and different parts of P. guajava have better antibacterial effect to V. coralliilyticus V. harveyi and P. damselae subsp piscicida and the and are 0-3 gml - and 0 - gml - respectively. Antibacterial effect of P. guajava is leaves > fruits > branches and of A. lancea is bran fry > salt fry > unprocessed > vinegar fry. Key words: Psidium guajava; Atractylodes lancea; aquatic pathogenic bacteria : ; : : ( CARS-48 ) ; ( 200B ) ; ( A2000H04) ; ( 200B ) : ( ) wrxlxw@ 2cn. com : ( ) com

2 5 : 33 ( Haliotis diversicolor) 2] ; - 5] TOS ( Trachinotus ovatus) 22] ; B2 BHI ( Babylonia areolata) ; Y3 ( Scylla serra- ta) ; Bw2 ; Yr2 6]. 2 23] ] 40 ( Solanum nigrum) ( Penaeus 20 g vannamei) ( P. monodon) ( Vibrio harveyi) 8] h 4] 500 ml 20 ml ( gml - ) 7-2] rmin - 5 min ] h 4] 5] h 2 ml 0 7 cfuml ( V. alginolyticus) 2% ( NaCl) ] 96 0 ( V. parahaemolyticus) gml gml - ( V. vulnificus) ( V. anguillarum) 30 L 30 L 6-8] 9-20] h ( Psidium guajava) ( Atractylodes lancea) ( OD) 600 nm 00 L 2 28 ( V. coralliilyticus NA030) 24 h 2 ( Photobacterium damselae subsp piscicida TOS) ( B2BHI) ( Y3) ( ) ( Morganella fulton Bw2) ( Yr2) 7 ( ) ( ) 7 ( 2% h ( ) ( ) ( ) 200 ml ( khz) NaCl) gml L L.. 2 NA h

3 34 9 NA030B2Y3TOS BHI OD ; 0-2 gml - NA030 OD ( - a) 0 - gml - 2 NA030B2 BHI OD ; 0-2 gml - OD 2. ( - b) OD OD NA030 B2 ( 96. 4% ) TOS BHI NA030B2 ( ) NA030 B2 TOS BHI; BHI 0 - gml - BHI NA030B h Tab. Bacteria cultivated for 24h on 96 pore plates strains leaves of guava Atractylodes 0 - gml gml gml gml - NA030( V. coralliilyticus NA030) Y3 V. parahaemolyticus Y B2 V. harveyi B TOS P. damselae subsp piscicida TOS BW2 M. fulton Bw BHI V. harveyi BHI YR2 V. vulnificus Yr ( a) ( b) Fig. OD of bacterium suspension containing extract from guava leaves ( a) and Atractylodes ( b)

4 5 : ml - ; gml - ; 4 ( NA030TOSB2 BHI) 2 ( 0 ) 0-2 gml - ml gml - 4 NA gml gml - TOS 0 - gml g B2 BHI NA g > > 2 4 Tab. 2 and for the Chinese herbal medicine to 4 pathogenic bacteria of seawater-cultured animals in vitro gml - Chinese herb NA030 TOS V. cosalliilyticus P. damselae NA030 subsp piscicida TOS B2 V. harveyi B2 BHI V. harveyi BHI leaves of guava branches of guava 0-2 > > > > 0 - fruits of guava > > > 0 - Atractylodes extracted by decocion Atractylodes extracted by cauterize with vinegar Atractylodes extracted by stir-baking with bran Atractylodes extracted by cauterize with salt 0-2 > > > > > > > > > > > > > > > 0 - BHI 0 - gml gml - ; NA030 TOS 0-2 gml - TOS B2 0-2 gml gml - ( Staphylococcus aureaus) 0 - gml - ; BHI NA g ml gml - ; gml - > > > 3 9] ( Escherchia coli) ( Neisseria meningitidis) ( Monilia albican) ( Pseudo- 25] monas Aeruginosa) ( Salmonella sp. ) 26] 24]

5 ]. J] ( 6) : ]. 8 Kirby-Bauer( K-B) 27] J] ( ) : ]. K-B J]. 20 7( 2) : ]. 6 4 J] ( 2 ) : 73 - ; 76. 3]. ; J] ( 8) : ] 4]. J] ( 5) : ]. CO 2 J]. 200 ( 5) : ]. ( Pseudosciaena crocea) 3 J]. OD ( ( 2) : ) OD 7]. ( J] ( 6) : % ) 8]. ( ) J] ( 2) : ]. 3 J] ( 9) : ]. J]. 2007( 22) : 424. : 22] WANG Ruixuan FENG Juan SU Youlu et al. Studies on the i- ]. solation of Photobacterium damselae subsp. piscicida from diseased J] ( 5 ) : golden pompano ( Trachinotus ovatus Linnaeus) and antibacterial a- 2]. J] ( 5) : ]. 23]. M]. : J] ( 2) : : ]. J]. 24] ( ) : ]. J]. 25] ( 4) : 6 -. J] ( 2) : ]. J] ( 4) : J] ( 3 ) : ]. 27] Clinical and Laboratory Standards Institute. Performance standards J] ( 2) : ] LIU Huawei TONG Jianming ZHOU Daowe et al. Utilization of Chinese herbal feed additives in animal production J]. Scientia Agricultura Sinica 20 0( 8) : ]. J] ( 4 ) : gents sensitivity J]. Vet Microbiol ( 2 /3 /4) : J] ( 3) : ]. for antimicrobial susceptibility testing; Sixteenth informational supplement S]. CLSI document M00 -S6 ( ISBN ). Wayne Pa USA: Clinical and Laboratory Standards Institute 2006.

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