Title of the study. Presentation of the first results of the entomological study
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1 Knowledge Translation Platform Feedback: From the last dengue fever season to a better prevention Title of the study Presentation of the first results of the entomological study Present by Dr. Didot PRASETYO Entomology supervisor from US NAMRU 2 1 Intervenant Titre de la présentation jj/mm/aaaa
2 Activities implemented Entomological Study in Kampong Chnang & Kampong Speu - Species inventory (conducted by NAMRU-2 & IPC) Mosquito trapping and species identification to obtain list of mosquito species that present in the area - Mosquito population dynamic (conducted by IPC) Monitor the changes in total mosquito number/biomass for a certain period of time 2 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
3 Species Inventory Mosquito habitat differences (indoor/outdoor) 3 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
4 Species Inventory Mosquito Active time differences (day/night) Aedes (Stegomyia) Culex Anopheles Culex 4 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
5 Species Inventory: methods Differences in outdoor/indoor preferences and active time deployment of several kind mosquito traps Traps used in this study: 1. Backpack aspirator 2. BG sentinel traps 3. Light Traps 4. Double Bed Net 5 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
6 Species Inventory: methods 1. Backpack Aspirator - Works like vacuum - Used in 10 randomly selected house per village - 10 minutes per house - Capture indoor host seeking & resting mosquito 6 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
7 Species Inventory: methods 2. BG sentinel traps - Using human skin chemical attract mosquito - Deployed one trap per village - 24 hours collection - Capture indoor host seeking mosquito 7 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
8 Species Inventory: methods How BG Trap works? By mimicking human odor to attract mosquito Attracted mosquito will fly near the trap, and then sucked into collecting net by fan odor odor odor odor odor odor 8 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
9 Species Inventory: methods 3. Light Traps - Using two attractans : Light and CO2 - Deployed two traps per village - Capture night host seeking mosquito 9 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
10 Species Inventory: methods How Light Trap works? CO2 Naturally attracted to light attracted to CO2 from Dry Ice CO2 10 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
11 Species Inventory: methods 4. Double bed Net - Using human as bait - inner layer to protect human bait - outer layer of net have approximately 10 cm of opening to trap mosquito - Trapped mosquito were collected by 2 nd volunteer using mouth aspirator - Deployed six traps per village with both day ( ) and night ( ) collection 11 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
12 Species Inventory: Results 1. In total 2001 mosquitoes collected at villages in Kampong Chnang Province - Backpack Aspirator: 2.2 mosquitoes per house - BGS trap: 3.5 mosquitoes per trap per 24 hours - Light Trap: 69.4 mosquitoes per trap per 12 hours - Double bed net day time: 7.5 mosquitoes per trap per 11 hours - Double bed net night time: 30.4 mosquitoes per trap per 4 hours 2. Consists of 31 Species in 10 Genera 12 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
13 Species Inventory: Results Mosquitoes in Kampong Chnang villages Dengue & CHIK vector CHIK vector Secondary malaria vector Possible malaria vector Species Proportion (%) Total Number Aedes (stegomyia) aegypti Aedes (stegomyia) albopictus Aedes (Aedimorphus) mediolineatus Aedes (Fredwardsius) vittatus Anopheles (cellia) phillipinensis Anopheles (cellia) karwari Anopheles (cellia) vagus Anopheles (Anopheles) argyropus Anopheles (Anopheles) barbirostris Group Anopheles (Anopheles) nigerimus Anopheles (Anopheles) nitidus 0 1 Anopheles (cellia) kochi 0 1 Anopheles (cellia) subpictus 0 1 Anopheles (cellia) spp Unidentified Anopheles (Anopheles) spp Unidentified Japanese Encephalitis Filariasis Culex vishnui Group Culex whitmorei Culex fuscocephala Culex gelidus Culex tritaeniorhynchus Culex bitaeniorhynchus Culex sinensis Culex quinquefasciatus Culex nigropunctatus 0 1 Culex brevipalpis 0 1 Culex spp Unidentified Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
14 Species Inventory: Results Mosquitoes in Kampong Chnang villages Species Proportion (%) Total Number Filariasis Filariasis Mansonia uniformis Mansonia annulifera Mansonia spp Armigeres subalbatus Armigeres spp Leicesteria spp Uranotaenia spp Verralina spp Zeugnomyia spp Coquillettidia crassipes Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
15 Species Inventory: Results Mosquitoes in Kampong Chnang Hospital Backpack Aspirator Dengue & CHIK vector Possible malaria vector Filariasis Japanese Enchepalitis Species Proportion (%) Total Number Aedes Aedes (stegomyia) aegypti Anopheles Anopheles (Anopheles) campestris Anopheles (cellia) vagus Anopheles (cellia) spp Culex Culex quinquefasciatus Culex tritaeniorhynchus Culex spp TOTAL 204 Light Trap Japanese Enchepalitis Filariasis Species Proportion (%) Total Number Culex tritaeniorhynchus Culex quinquefasciatus Culex gelidus TOTAL Intervenant Titre de la présentation jj/mm/aaaa
16 Species Inventory: Results Mosquitoes in Kampong Speu Hospital Dengue & CHIK vector Possible malaria vector Filariasis and Japanese Encephalitis vector Backpack Aspirator Species Proportion (%) Total Number Aedes Aedes (stegomyia) aegypti Anopheles Anopheles (cellia) vagus Anopheles (cellia) spp Culex Culex quinquefasciatus Culex gelidus Culex vishnui Group TOTAL 235 Dengue & CHIK vector Secondary malaria vector Japanese encephalitis Filariasis Filariasis Light Trap 16 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014 Species Proportion (%) Total Number Aedes Aedes (aedimorphus) mediolineatus Aedes (stegomyia) aegypti Anopheles Anopheles (cellia) phillipinensis Culex Culex fuscocephala Culex tritaeniorhynchus Culex gelidus Culex nigropunctatus Culex quinquefasciatus Culex whitmorei Mansonia Mansonia uniformis Mansonia annulifera Mansonia spp Coquillettidia TOTAL 126
17 Species Inventory: Results Backpack aspirator (indoor mosquitoes) in K Chnang villages Total captured : 133 Culex 17 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
18 Species Inventory: Results BGS Trap (Human skin chemical) in K Chnang villages Total captured : 35 17% 18 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
19 Species Inventory: Results Light Trap (Light and CO2) in K Chnang villages Total captured : 694 <1% 19 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
20 Species Inventory: Results Double Bed Nets (Human bait) in K Chnang villages Total captured: 226 (Day), 913 (Night) 8% <1% 20 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
21 Species Inventory: Conclusion Points to remember! Dengue and Chikungunya vector (Aedes aegypti & Aedes albopictus) captured in higher proportion using BGS trap, Backpack Aspirator, and Double Net (Day time) Light Trap and Double net (Night Time) captured lower proportion of Dengue and Chikungunya vector 21 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
22 Population dynamic: Methods Mosquito Population Dynamic study (On going) - Using double bed net methods - Deployed six traps per village with night time collection ( ) - Period of collection: 15 days per month - Total number / biomass will be observed 22 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
23 Population dynamic: Results Kampong Chnang (Oct 2014) Kampong Speu (Oct 2014) 23 Intervenant Titre de la présentation jj/mm/aaaa
24 Population dynamic: Conclusion Mosquito Population Dynamic study - Total mosquito number/biomass data for more than one month is required for better visualisation of population dynamic Future Steps - Continuing total mosquito number/biomass calculation - Observing correlation between mosquito population dynamic and disease incidence in every village 24 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
25 Thank you for your attention! 25 Dr. Didot Prasetyo Presentation of the first results of the entomological study 14/11/2014
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