Model 125 Electrophoresis Power Supply

Transcription

1 Instruction Manual Model 125 Electrophoresis Power Supply CAT. SERIES 31061

2 Table of Contents 1. Introduction Notice to Customer Warnings Overview Description Set-Up Operating Instructions Troubleshooting Guide Related Products Additional Information Care and Handling Specifications Warranty Declaration of Conformity and CE Mark...15 Figures 1. Model 125 Electrophoresis Power Supply Model 125 Rear and Bottom Views... 6 HORIZON, SUNRISE, TECH-LINE SM, and the Life Technologies logo are marks of Life Technologies, Inc. i

3 1 Introduction 1.1 Notice to Customer This product is authorized for laboratory research use only. The product has not been qualified or found safe and effective for any human or animal diagnostic or therapeutic application. Uses for other than the labeled intended use may be a violation of applicable law. If the product is used in a manner not specified by the manufacturer, the protection provided by the product may be impaired. 1.2 Warnings 1. DANGER! HIGH VOLTAGE! This power supply has been designed for use as a DC power source for electrophoresis. It is capable of generating lethal currents. This unit should always be operated with extreme caution. Careless handling could result in electrical shock. 2. Never operate damaged equipment. Do not open the power supply. There are no userserviceable parts inside. If the power supply emits smoke or fails to operate, turn off the unit and disconnect the AC line and power cords. 3. Do not operate with DC power cords that have exposed live wires. 1

4 4. Always turn off the power supply before connecting or removing the DC power cords or moving the electrophoresis apparatus. 5. Handle one power cord at a time, with one hand only, when connecting or removing the DC power cords at the power output terminals. 6. Do not immerse the power supply in water. 7. Do not operate the power supply in a damp, humid atmosphere or in a fashion where condensed moisture may short out electrical components. 8. Do not connect the DC output to earth ground. 9. The AC power cord has a three-pronged plug which must be connected to a grounded line voltage receptacle. Do not use a two-wire receptacle with an adapter. This could create a serious electrical hazard for persons using the power supply. 10. Use the correct AC line voltage for the power supply. Be sure to always plug a 115 VAC power supply into a 115 V line and use a 230 V line with a 230 VAC power supply. The AC line voltage selector switch is located on the bottom of the power supply. 2

5 2 Overview 2.1 Description The Model 125 Electrophoresis Power Supply provides controlled DC output of 50, 100, and 125 V. The power supply operates in constant voltage mode, at currents up to 150 ma. The single set of recessed output terminals allows for one electrophoresis apparatus to be run at a time. Safety features include a PTC (Positive Thermal Coefficient) device that automatically resets when a fault condition is removed. 3

6 0 Power on indicator Power switch (On/Off) I MODEL 125 Input Voltage ~ / VAC 50W, Hz LIFE GIBCOBRL TECHNOLOGIES Electrophoresis Power Supply 100 Output DC Voltage Output voltage selector switch Negative (-) output Positive (+) output Figure 1. Model 125 Electrophoresis Power Supply. 4

7 Overview 2.2 Set-Up Unpacking the Power Supply. Unpack and inspect the power supply carefully for any damage. Do not use the power supply if it is damaged. If the power supply is damaged, save the packing material and report the problem to Customer Service. Location. Make sure the power supply is set up in a location where it is protected from physical damage, moisture, corrosive agents, and extreme temperatures. The power supply should be readily accessible for safe operation. Connection with the AC Mains. Make sure the line selector switch located on the bottom of the unit (figure 2) is set to the correct line voltage (115/230 VAC). Connect the power supply to the AC mains with the correct line voltage. Make sure the main receptacle and the power supply have the proper three-wire (grounded) connections. 5

8 AC input receptacle Fuse tray Mains voltage selection switch Figure 2. Model 125 Rear and Bottom Views. 6

9 3 Operating Instructions Read this section in its entirety before setting up or operating the Model 125 Electrophoresis Power Supply. 1. Place the Model 125 Power Supply, with the electrophoresis apparatus, on a level surface in a safe, dry, isolated area. When moving the power supply from room temperature into a cold room or vice versa, allow at least 8 h for the power supply to equilibrate before use. Note: Allow sufficient space for the electrophoresis apparatus to be connected with the power supply. Place and orient the power supply in such a manner that the controls on the front and top panels are easily accessible. Post warning signs to indicate that high voltage is present. 2. Make sure that the power switch located on the top panel of the power supply is in the off position, indicated by the symbol O (figure 1). 3. Place the AC power cord into the threepronged inlet receptacle located on the rear panel (figure 2). 7

10 Note: The AC power cord has a threepronged plug. This must be connected to a grounded line voltage receptacle. Do not use a two-wire receptacle with an adapter. This could create a serious electrical hazard for persons using the power supply. 4. Check to ensure that the line selector switch located on the bottom of the power supply (figure 2) is set to the proper voltage (115/230 VAC). 5. Plug the AC power cord into an outlet of the proper line voltage. Note: Do not plug the power supply set for 115-V into a 230-V line. 6. Connect the Model 125 Power Supply to the electrophoresis apparatus using the DC power cords. The power cords are color-coded: the black is negative, and the red is positive. 7. Set the voltage selector (located on the front panel, figure 1) to the desired output voltage of 50, 100, or 125 VDC. 8. Turn the power switch (located on the upper left corner of the front panel, figure 1) to the on position. The green power indicator light will illuminate. The DC output is now on, and electrophoresis starts in the apparatus connected to the power supply. 9. Determine that the power supply is working properly by watching for tiny bubbles on the electrodes in the buffer tray of the electrophoresis apparatus. 8

11 Operating Instructions 10. When the electrophoresis run is complete, turn the power switch to the off position. 11. Disconnect the Model 125 Power Supply from the wall outlet. 12. Disconnect the power cords from the Model 125 Power Supply and the electrophoresis apparatus. 9

12 4 Troubleshooting Guide Many operating problems may be solved by carefully following the instructions in this manual. Some suggestions for troubleshooting are included here. Should these suggestions not resolve the problem, the unit should be returned to Life Technologies for repair. A full description of the problem should be included. Contact the Customer Service Department for shipping instructions. Questions regarding procedures should be directed to the Life Technologies TECH-LINE SM. Customers outside of the United States should contact their local distribution center for information regarding repairs. Problem Comments Power supply is not supplying DC voltage upon start up Verify that the AC power cord is plugged into a working outlet of the proper voltage. Verify that the line selector switch is set to the correct voltage. Verify that the power switch is in the on position. During operation, the green light goes out The Model 125 is equipped with a replaceable current fuse (T0.5A/250V 5 x 20 mm for both 115/230 VAC). Disconnect the power cord from the power supply before checking or exchanging the fuse. The fuseholder tray is located beneath the AC input receptacle on the rear panel of the power supply. The fuseholder has two tabs which need to be gripped in order to remove the fuse. If the fuse is blown (black spot), it needs to be replaced. Insert the new fuse into the tray and push the assembly back into the AC input module. 10

13 Troubleshooting Guide Problem During operation, power supply stops supplying DC voltage (but the green light remains on) Comments If a fault condition, i.e., short-circuit or current overload, occurs on the DC output, the PTC (Positive Temperature Coefficient) device will activate and impede current flow. Rotate the power (I/O) rocker switch to off position, correct the fault condition, and allow the unit to cool down. The PTC safety device will automatically reset to normal resistance mode. Slide the voltage selector switch to its proper position, expos- ing the desired voltage setting (115/230 VAC). Line selector switch is not set to local voltage Positive or negative power cords will not fit in banana jacks tightly Verify that the banana jacks are the appropriate size. 11

14 5 Related Products Product Cat. No. Apparatus: HORIZON 58 Horizontal Gel Electrophoresis Apparatus SUNRISE Horizontal Electrophoresis Apparatus HORIZON Horizontal Gel Electrophoresis Apparatus HORIZON Horizontal Gel Electrophoresis Apparatus Mini-V 8 10 Vertical Gel Electrophoresis Apparatus Replacement Fuse, T0.5A/250V Reagents: Agarose, 100 g Agarose, 500 g X TBE Buffer, 1 L X TBE Buffer, 4 L X TAE Buffer, 1 L X TAE Buffer, 4 L

15 6 Additional Information 6.1 Care and Handling The Model 125 Electrophoresis Power Supply is constructed from flame-retardant ABS plastic. The power and voltage selector switches are made of molded plastic. The power supply must not be immersed in water. The unit can be wiped with a soft cloth, dampened with water and a nonabrasive, mild soap or detergent. Do not allow water to enter the case. Abrasive cleaners, window sprays or rough cloths may damage the surface and should be avoided. Grease and oils may be removed using a light application of hexane, kerosene or aliphatic naphtha. Do not expose the surface to phenol, acetone, benzene, halogenated hydrocarbon solvents or undiluted laboratory alcohols. Avoid prolonged exposure of the power supply to UV light. A soft, dry cloth may be used to dry the unit. 6.2 Specifications Installation Category II Net Weight: 0.8 kg (1.7 lb.) Shipping Weight: 0.85 kg (1.9 lb.) Dimensions (D x W x H) 12.1 x 7.6 x 5.7 cm (4.7 x 3.0 x 2.2 in.) Enclosure Construction: Injection molded ABS plastic, ultrasonically sealed Power Requirement: 115/230 VAC, 50/60 Hz, 50 W Fuses: T0.5A/250V 5 x 20 mm for 115/230 VAC Operating Temperature Range: 4 C - 30 C (non-condensing atmosphere) DC Output Voltage: 50, 100, 125 VDC DC Output Current Range: ma AC Power Cord: 91.4 cm (36 in.) 13

16 6.3 Warranty Life Technologies, Inc. warrants apparatus of its manufacture against defects in materials and workmanship, under normal service, for one year from the date of receipt by the purchaser. This warranty excludes damages resulting from shipping, misuse, carelessness, or neglect. Life Technologies liability under the warranty is limited to the repair of such defects or the replacement of the product, at its option, and is subject to receipt of reasonable proof by the customer that the defect is embraced within the terms of the warranty. All claims made under this warranty must be presented to Life Technologies within one year following the date of delivery of the product to the customer. This warranty is in lieu of any other warranties or guarantees, expressed or implied, arising by law or otherwise. Life Technologies makes no other warranty, expressed or implied, including warranties of merchantability or fitness for a particular purpose. Under no circumstances shall Life Technologies be liable for damages either consequential, compensatory, incidental or special, sounding in negligence, strict liability, breach of warranty or any other theory, arising out of the use of the product listed herein. Life Technologies reserves the right to make improvements in design, construction, and appearance without notice. 14

17 Additional Information 6.4 Declaration of Conformity and CE Mark Note: The information outlined in this section applies only to customers located in the European Union (EU). The EU is currently comprised of 15 member countries. This laboratory apparatus is identified with the CE mark. This mark indicates that the product complies to the following EU Directives and Standards: Application of Council Directive(s): 89/336/EEC Electromagnetic Compatibility 73/23/EEC Low Voltage Directive Standards: EN :1992 Emissions EN :1992 Immunity EN :1993 Product Safety EU Representative: Life Technologies Ltd. EU Address: 3 Fountain Dr. Inchinnan Business Park Paisley, PA49RF Scotland A copy of the Declaration of Conformity certificate is available upon request. 15

18 Part No Lot. No. NBWP