The correct answer is c B. Answer b is incorrect. Type II enzymes recognize and cut a specific site, not at random sites.

Size: px
Start display at page:

Download "The correct answer is c B. Answer b is incorrect. Type II enzymes recognize and cut a specific site, not at random sites."

Transcription

1 1. A recombinant DNA molecules is one that is a. produced through the process of crossing over that occurs in meiosis b. constructed from DNA from different sources c. constructed from novel combinations of DNA from the same source d. produced through mitotic cell division A. Answer a is incorrect. Crossing over produces novel combinations of DNA from a single individual. The offspring are called recombinants. constructed from DNA from different sources B. Answer b is correct. Recombinant DNA is constructed in a laboratory and manipulated for a specific purpose. C. Answer c is incorrect. New genetic combinations from the same individual occur as a product of crossing over during meiosis. Recombinant DNA is an artificial construct that uses DNA from different sources. D. Answer d is incorrect. Mitosis is the asexual form of cell division that produces genetically identical daughter cells. There is no recombination of DNA during mitosis. 2. Type II restriction endonucleases are useful because a. they degrade DNA from the 5 end b. they cleave the DNA at random locations c. they cleave the DNA at specific sequences d. they only cleave modified DNA A. Answer a is incorrect. The ability to cleave DNA from an end is a property of an exonuclease. Endonucleases work by breaking bonds within the DNA molecule. B. Answer b is incorrect. Type II enzymes recognize and cut a specific site, not at random sites. they cleave the DNA at specific sequences C. Answer c is correct. A type II restriction endonuclease works by cleaving the DNA at specific sequences. This property is used in many experimental techniques that involve the manipulation of DNA. D. Answer d is incorrect. The cells that produce these enzymes protect their own DNA because they do not work on modified DNA. 3. What is the basis of separation of DNA fragments by gel electrophoresis? a. The negative charge on DNA b. The size of the DNA fragments

2 c. The sequence of the fragments d. The presence of a dye A. Answer a is incorrect. The negative charge is necessary to cause the movement of the DNA fragments through the gel. The size of the DNA fragments B. Answer b is correct. Different size fragments move at different speeds with small fragments moving more quickly than larger fragments. C. Answer c is incorrect. The sequence of the fragments does not contribute to the separation. D. Answer d is incorrect. Dyes are used to visualize the fragments, but they do not contribute to the separation. 4. How is the gene for β-galactosidase used in the construction of a plasmid? a. The gene is a promoter that is sensitive to the presence of the sugar, galactose. b. It is an origin of replication. c. It is a cloning site. d. It is a marker for insertion of DNA. A. Answer a is incorrect. Promoters are binding sites for RNA polymerase. B. Answer b is incorrect. Beta-galactosidase is an enzyme not an origin of replication. C. Answer c is incorrect. The gene for β-galactosidase is located in cloning sites, but is not itself a cloning site. It is a marker for insertion of DNA. D. Answer d is correct. When DNA is inserted into the plasmid, the function of this enzyme is disrupted. The absence of color formation is a marker for recombination. 5. The basic logic of enzymatic DNA sequencing is to produce a. a nested set of DNA fragments produced by restriction enzymes b. a nested set of DNA fragments that each begin with different bases c. primers to allow PCR amplification of the region between the primers d. a nested set of DNA fragments that end with known bases A. Answer a is incorrect. Restriction enzymes cut specific sites but not frequently enough to allow sequencing.

3 B. Answer b is incorrect. It is important during enzymatic sequencing that the fragments all begin from the same primer, because they are then terminated at different bases. C. Answer c is incorrect. PCR amplification is now used for sequencing, but the logic still requires generating a nested set of fragments that end with the same base. a nested set of DNA fragments that end with known bases D. Answer d is correct. The logic still requires generating a nested set of fragments that end with the same base. This is accomplished using nucleotides that act as chain terminators. 6. A DNA library is a. an orderly array of all the genes within an organism b. a collection of vectors c. the collection of plasmids found within a single E. coli d. a collection of DNA fragments representing the entire genome of an organism A. Answer a is incorrect. Genomic libraries are made up of random fragments of DNA from the entire genome, not just genes. B. Answer b is incorrect. A library is a collection of vectors with DNA inserted into them. C. Answer c is incorrect. Multiple cells are required to hold all the vectors that carry the various DNA inserts that make up a genomic library. a collection of DNA fragments representing the entire genome of an organism D. Answer d is correct. A genomic library is a collection of random DNA fragments from the entire genome. 7. Molecular hybridization is used to a. generate cdna from mrna b. introduce a vector into a bacterial cell c. screen a DNA library d. introduce mutations into genes A. Answer a is incorrect. The enzyme reverse transcriptase is used to make cdna from mrna. B. Answer b is incorrect. Molecular hybridization refers to the ability of a labeled probe to bind to its complementary sequence in denatured DNA. screen a DNA library

4 C. Answer c is correct. Molecular hybridization refers to the ability of a labeled probe to bind to its complementary sequence in denatured DNA. This property is used to identify the presence of genes of interest within large DNA collections like libraries. D. Answer d is incorrect. Molecular hybridization refers to the ability of a labeled probe to bind to its complementary sequence in denatured DNA. 8. The enzyme used in the polymerase chain reaction is a. a restriction endonuclease b. heat-resistant RNA polymerase c. reverse transcriptase d. a heat-resistant DNA polymerase A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. B. Answer b is incorrect. PCR generates DNA fragments, not RNA. C. Answer c is incorrect. Reverse transcriptase is used to convert RNA into DNA. PCR uses DNA as its template. a heat-resistant DNA polymerase D. Answer d is correct. PCR synthesizes DNA strands from a DNA template. The specific enzyme used, Taq polymerase, needs to be heat-resistant because the reaction cycles through high- and low-temperature phases. 9. How does the yeast two-hybrid system detect protein protein interactions? a. Binding of fusion partners triggers a signal cascade that alters gene expression. b. Fusion partners are detected using radioactive probes of Western blots. c. Protein protein binding of fusion partners triggers expression of a reporter gene. d. Protein protein binding of fusion partners triggers expression of the Gal4 gene. A. Answer a is incorrect. The fusion partners are two parts of the Gal4 transcriptional activator. No signal cascade is involved. B. Answer b is incorrect. The fusion partners are two parts of the transcriptional activator, Gal4. Gal4 activity results in a change in gene expression. Protein protein binding of fusion partners triggers expression of a reporter gene. C. Answer c is correct. If the fusion partners can bind, then Gal4 becomes functional and will trigger the expression of a reporter gene linked to the Gal4 promoter.

5 D. Answer d is incorrect. The fusion partners are hybrid proteins linking part of the Gal4 transcription activator with a protein of interest. Fusion partner binding triggers expression of a gene downstream from the Gal4 promoter. 10. In vitro mutagenesis is used to a. produce large quantities of mutant proteins b. create mutations at specific sites within a gene c. create random mutations within multiple genes d. create organisms that carry foreign genes A. Answer a is incorrect. The production of large quantities of proteins is made possible by expression vectors. create mutations at specific sites within a gene B. Answer b is correct. Scientists use in vitro mutagenesis to examine the effect of specific changes in gene sequence of the function of the protein product. C. Answer c is incorrect. In vitro mutagenesis allows for the generation of known mutations. D. Answer d is incorrect. Transgenic organisms carry foreign genes. 11. Insertion of a gene for a surface protein from a medically important virus such as herpes into a harmless virus is an example of a. a DNA vaccine b. reverse genetics c. gene therapy d. a subunit vaccine A. Answer a is incorrect. A DNA vaccine is based on splicing an internal protein into a plasmid to trigger a cellular immune response. B. Answer b is incorrect. Reverse genetics refers to the use of genetic knockouts to examine the role of a specific gene. C. Answer c is incorrect. Gene therapy involves the integration of a functional gene into an organism to replace or repair an existing genetic mutation. a subunit vaccine D. Answer d is correct. Subunit vaccines are based on packaging a gene for a unique marker protein for a pathogenic virus within a nonpathogenic virus which protects the individual by priming the immune response without exposing the person to the dangerous virus.

6 12. What is a Ti plasmid? a. A vector that can transfer recombinant genes into plant genomes b. A vector that can be used to produce recombinant proteins in yeast c. A vector that is specific to cereal plants like rice and corn d. A vector that is specific to embryonic stem cells The correct answer is a A vector that can transfer recombinant genes into plant genomes A. Answer a is correct. The Ti plasmid can trigger the integration of the genes within the plasmid into a plant cell s chromosome. In this way all the cells that arise from the transfected cell will carry the recombinant protein. The correct answer is a B. Answer b is incorrect. The Ti plasmid is specific to the plant bacteria Agrobacterium. The correct answer is a C. Answer c is incorrect. The Ti plasmid normally does not transfecting cereal plants. It has now been modified to allow use with cereals. The correct answer is a D. Answer d is incorrect. The Ti plasmid is specific for plants. 13. Which of the following is NOT a possible benefit of genetically modified crops? a. Increased nutritional value for people b. Enhanced resistance to insect pests c. Enhanced resistance to broad spectrum herbicides d. Enhanced resistance to insecticides A. Answer a is incorrect. Golden Rice is an example of a genetically modified crop with improved nutritional properties for humans. B. Answer b is incorrect. Bt crops are genetically modified to produce insecticidal proteins. C. Answer c is incorrect. Crops genetically engineered to overexpress EPSP synthase are an example of a plant that will resist herbicides. Enhanced resistance to insecticides D. Answer d is correct. Plants do not have to resist insecticides. Challenge Questions 1. Many human proteins, such as hemoglobin, are only functional as an assembly of multiple subunits. Assembly of these functional units occurs within the endoplasmic reticulum and Golgi apparatus of a eukaryotic cell. Discuss what limitations, if any, exist to the large-scale production of genetically engineered hemoglobin.

7 Answer Genetic engineering often means insertion of a gene of interest (and harvest of the protein of interest) from vast numbers of bacterial cells. Bacteria are prokaryotes, and as such do not have internal membrane systems like the endoplasmic reticulum and Golgi apparatus. An alternative would be to use a yeast system since yeast cells are eukaryotes. In either case, one of the major limiting factors is the ability to efficiently harvest the protein of interest and purify it away from all the other proteins normally produced by the host cell. 2. Enzymatic sequencing of a short strand of DNA was completed using dideoxynucleotides. Use the gel shown to determine the sequence of that DNA. G C A T Answer Read the gel starting from the smallest fragment (at the bottom) to the largest. The sequence is: CTGATAGTCAGCTG

Chapter 12 - DNA Technology

Chapter 12 - DNA Technology Bio 100 DNA Technology 1 Chapter 12 - DNA Technology Among bacteria, there are 3 mechanisms for transferring genes from one cell to another cell: transformation, transduction, and conjugation 1. Transformation

More information

CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA TECHNOLOGY

CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA TECHNOLOGY CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA TECHNOLOGY I. General Info A. Landmarks in modern genetics 1. Rediscovery of Mendel s work 2. Chromosomal theory of inheritance 3. DNA as the genetic material

More information

Tools and Techniques. Chapter 10. Genetic Engineering. Restriction endonuclease. 1. Enzymes

Tools and Techniques. Chapter 10. Genetic Engineering. Restriction endonuclease. 1. Enzymes Chapter 10. Genetic Engineering Tools and Techniques 1. Enzymes 2. 3. Nucleic acid hybridization 4. Synthesizing DNA 5. Polymerase Chain Reaction 1 2 1. Enzymes Restriction endonuclease Ligase Reverse

More information

Recombinant DNA Technology

Recombinant DNA Technology PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 8 Recombinant DNA Technology The Role of Recombinant DNA Technology in Biotechnology Biotechnology

More information

Recombinant DNA and Biotechnology

Recombinant DNA and Biotechnology Recombinant DNA and Biotechnology Chapter 18 Lecture Objectives What Is Recombinant DNA? How Are New Genes Inserted into Cells? What Sources of DNA Are Used in Cloning? What Other Tools Are Used to Study

More information

Chapter 9. Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA

Chapter 9. Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA Chapter 9 Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA Q&A Interferons are species specific, so that interferons to be used in humans must be produced in human cells. Can you think

More information

Genetics Faculty of Agriculture and Veterinary Medicine

Genetics Faculty of Agriculture and Veterinary Medicine Genetics 10201232 Faculty of Agriculture and Veterinary Medicine Instructor: Dr. Jihad Abdallah Topic 15:Recombinant DNA Technology 1 Recombinant DNA Technology Recombinant DNA Technology is the use of

More information

Recipient Cell. DNA Foreign DNA. Recombinant DNA

Recipient Cell. DNA Foreign DNA. Recombinant DNA Module 4B Biotechnology In this module, we will examine some of the techniques scientists have developed to study and manipulate the DNA of living organisms. Objective # 7 Explain what genetic recombination

More information

Chapter 10 Manipulating Genes

Chapter 10 Manipulating Genes How DNA Molecules Are Analyzed Chapter 10 Manipulating Genes Until the development of recombinant DNA techniques, crucial clues for understanding how cell works remained lock in the genome. Important advances

More information

Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA Recombinant DNA procedures - an overview Biotechnology: The use of microorganisms, cells, or cell components to make a product. Foods, antibiotics, vitamins, enzymes Recombinant

More information

Chapter 20: Biotechnology: DNA Technology & Genomics

Chapter 20: Biotechnology: DNA Technology & Genomics Biotechnology Chapter 20: Biotechnology: DNA Technology & Genomics The BIG Questions How can we use our knowledge of DNA to: o Diagnose disease or defect? o Cure disease or defect? o Change/improve organisms?

More information

Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company

Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Biotechnology and reporter genes Here, a lentivirus is used to carry foreign DNA into chickens. A reporter gene (GFP)indicates that foreign DNA has been successfully transferred. Recombinant DNA continued

More information

Name Class Date. KEY CONCEPT Mutations are changes in DNA that may or may not affect phenotype. frameshift mutation

Name Class Date. KEY CONCEPT Mutations are changes in DNA that may or may not affect phenotype. frameshift mutation Unit 7 Study Guide Section 8.7: Mutations KEY CONCEPT Mutations are changes in DNA that may or may not affect phenotype. VOCABULARY mutation point mutation frameshift mutation mutagen MAIN IDEA: Some mutations

More information

Recombinant DNA & Genetic Engineering. Tools for Genetic Manipulation

Recombinant DNA & Genetic Engineering. Tools for Genetic Manipulation Recombinant DNA & Genetic Engineering g Genetic Manipulation: Tools Kathleen Hill Associate Professor Department of Biology The University of Western Ontario Tools for Genetic Manipulation DNA, RNA, cdna

More information

restriction enzymes 350 Home R. Ward: Spring 2001

restriction enzymes 350 Home R. Ward: Spring 2001 restriction enzymes 350 Home Restriction Enzymes (endonucleases): molecular scissors that cut DNA Properties of widely used Type II restriction enzymes: recognize a single sequence of bases in dsdna, usually

More information

Today-applications: Medicine-better health Pharmaceutical-production of antibiotics Foods-wine, cheese, beer Agriculture-selective breeding

Today-applications: Medicine-better health Pharmaceutical-production of antibiotics Foods-wine, cheese, beer Agriculture-selective breeding I. Genetic Engineering modification of DNA of organisms to produce new genes with new characteristics -genes are small compared to chromosomes -need methods to get gene-sized pieces of DNA -direct manipulation

More information

CHAPTER 8 RECOMBINANT DNA and GENETIC ENGINEERING

CHAPTER 8 RECOMBINANT DNA and GENETIC ENGINEERING CHAPTER 8 RECOMBINANT DNA and GENETIC ENGINEERING Questions to be addressed: How are recombinant DNA molecules generated in vitro? How is recombinant DNA amplified? What analytical techniques are used

More information

2. Enzymes that cleave DNA at specific sites are called.

2. Enzymes that cleave DNA at specific sites are called. Biotechnology 1. The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the fate of organisms. 2. Enzymes that

More information

Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College

Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Primary Source for figures and content: Eastern Campus Tortora, G.J. Microbiology

More information

Biotechnology Test Test

Biotechnology Test Test Log In Sign Up Biotechnology Test Test 15 Matching Questions Regenerate Test 1. Plasmid 2. PCR Process 3. humulin 4. pluripotent 5. polymerase chain reaction (PCR) a b Is much smaller than the human genome,

More information

Name Class Date WHAT I KNOW. organisms with specific traits for certain functions. For example, some plants provide food.

Name Class Date WHAT I KNOW. organisms with specific traits for certain functions. For example, some plants provide food. Genetic Engineering Science as a Way of Knowing Q: How and why do scientists manipulate DNA in living cells? 15.1 How do humans take advantage of naturally occurring variation among organisms? WHAT I KNOW

More information

Gene Cloning and DNA Analysis: An Introduction

Gene Cloning and DNA Analysis: An Introduction Gene Cloning and DNA Analysis: An Introduction Brown, Terry A. ISBN-13: 9781405111218 Table of Contents PART 1 THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS. Chapter 1 Why Gene Cloning and DNA

More information

CCR Biology - Chapter 9 Practice Test - Summer 2012

CCR Biology - Chapter 9 Practice Test - Summer 2012 Name: Class: Date: CCR Biology - Chapter 9 Practice Test - Summer 2012 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Genetic engineering is possible

More information

PART 1 THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS. Chapter 1 Why Gene Cloning and DNA Analysis are Important

PART 1 THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS. Chapter 1 Why Gene Cloning and DNA Analysis are Important TABLE OF CONTENTS PART 1 THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS Chapter 1 Why Gene Cloning and DNA Analysis are Important 1.1 The early development of genetics 1.2 The advent of gene cloning

More information

CHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA

CHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA CHAPTER 6: RECOMBINANT DNA TECHNOLOGY YEAR III PHARM.D DR. V. CHITRA INTRODUCTION DNA : DNA is deoxyribose nucleic acid. It is made up of a base consisting of sugar, phosphate and one nitrogen base.the

More information

Biotechnology in Medicine and Agriculture

Biotechnology in Medicine and Agriculture Biotechnology in Medicine and Agriculture Bởi: OpenStaxCollege It is easy to see how biotechnology can be used for medicinal purposes. Knowledge of the genetic makeup of our species, the genetic basis

More information

3. comparison with proteins of known function

3. comparison with proteins of known function Lectures 26 and 27 recombinant DNA technology I. oal of genetics A. historically - easy to isolate total DNA - difficult to isolate individual gene B. recombinant DNA technology C. why get the gene? 1.

More information

Microbiology / Active Lecture Questions Chapter 9 Biotechnology & Recombinant DNA 1 Chapter 9 Biotechnology & Recombinant DNA

Microbiology / Active Lecture Questions Chapter 9 Biotechnology & Recombinant DNA 1 Chapter 9 Biotechnology & Recombinant DNA 1 2 Restriction enzymes were first discovered with the observation that a. DNA is restricted to the nucleus. b. phage DNA is destroyed in a host cell. c. foreign DNA is kept out of a cell. d. foreign DNA

More information

Chapter 20: Biotechnology

Chapter 20: Biotechnology Name Period The AP Biology exam has reached into this chapter for essay questions on a regular basis over the past 15 years. Student responses show that biotechnology is a difficult topic. This chapter

More information

Biotechnology: DNA Technology & Genomics

Biotechnology: DNA Technology & Genomics Chapter 20. Biotechnology: DNA Technology & Genomics 2003-2004 The BIG Questions How can we use our knowledge of DNA to: diagnose disease or defect? cure disease or defect? change/improve organisms? What

More information

NAME: Microbiology BI234 MUST be written and will not be accepted as a typed document.

NAME: Microbiology BI234 MUST be written and will not be accepted as a typed document. Chapter 8 Study Guide What is the study of genetics, and what topics does it focus on? What is a genome? NAME: Microbiology BI234 MUST be written and will not be accepted as a typed document. Describe

More information

BIOTECHNOLOGY. What can we do with DNA?

BIOTECHNOLOGY. What can we do with DNA? BIOTECHNOLOGY What can we do with DNA? Biotechnology Manipulation of biological organisms or their components for research and industrial purpose Usually manipulate DNA itself How to study individual gene?

More information

DNA TECHNOLOGY- methods for studying and manipulating genetic material.

DNA TECHNOLOGY- methods for studying and manipulating genetic material. 1 DNA TECHNOLOGY- methods for studying and manipulating genetic material. BIOTECHNOLOGY, the manipulation of organisms or their components to make useful products. Biotechnology today usually refers to

More information

11/19/2008. Gene analysis. Sequencing PCR. Northern-blot RT PCR. Western-blot Sequencing. in situ hybridization. Southern-blot

11/19/2008. Gene analysis. Sequencing PCR. Northern-blot RT PCR. Western-blot Sequencing. in situ hybridization. Southern-blot Recombinant technology Gene analysis Sequencing PCR RNA Northern-blot RT PCR Protein Western-blot Sequencing Southern-blot in situ hybridization in situ hybridization Function analysis Histochemical analysis

More information

Recombinant DNA technology (genetic engineering) involves combining genes from different sources into new cells that can express the genes.

Recombinant DNA technology (genetic engineering) involves combining genes from different sources into new cells that can express the genes. Recombinant DNA technology (genetic engineering) involves combining genes from different sources into new cells that can express the genes. Recombinant DNA technology has had-and will havemany important

More information

Biotechnology. Selective breeding Use of microbes (bacteria & yeast)

Biotechnology. Selective breeding Use of microbes (bacteria & yeast) Biotechnology bio and technology The use of living organisms to solve problems or make useful products. Biotechnology has been practiced for the last 10,000 years. Selective breeding Use of microbes (bacteria

More information

Genetic Technology. Name: Class: Date: Multiple Choice Identify the choice that best completes the statement or answers the question.

Genetic Technology. Name: Class: Date: Multiple Choice Identify the choice that best completes the statement or answers the question. Name: Class: Date: Genetic Technology Multiple Choice Identify the choice that best completes the statement or answers the question. 1. An application of using DNA technology to help environmental scientists

More information

GENE CLONING AND RECOMBINANT DNA TECHNOLOGY

GENE CLONING AND RECOMBINANT DNA TECHNOLOGY GENE CLONING AND RECOMBINANT DNA TECHNOLOGY What is recombinant DNA? DNA from 2 different sources (often from 2 different species) are combined together in vitro. Recombinant DNA forms the basis of cloning.

More information

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology Lecture 13: DNA Technology DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology DNA Sequencing determine order of nucleotides in a strand of DNA > bases = A,

More information

DNA Fingerprinting. Unless they are identical twins, individuals have unique DNA

DNA Fingerprinting. Unless they are identical twins, individuals have unique DNA DNA Fingerprinting Unless they are identical twins, individuals have unique DNA DNA fingerprinting The name used for the unambiguous identifying technique that takes advantage of differences in DNA sequence

More information

Recombinant DNA Technology

Recombinant DNA Technology Recombinant DNA Technology Dates in the Development of Gene Cloning: 1965 - plasmids 1967 - ligase 1970 - restriction endonucleases 1972 - first experiments in gene splicing 1974 - worldwide moratorium

More information

RECOMBINANT DNA TECHNOLOGY

RECOMBINANT DNA TECHNOLOGY RECOMBINANT DNA TECHNOLOGY By; Dr. Adeel Chaudhary 2 nd yr Molecular Genetics Medical Technology College of Applied Medical Sciences Recombinant DNA is a form of artificial DNA that is made through the

More information

Genetics Module B, Anchor 3

Genetics Module B, Anchor 3 Genetics Module B, Anchor 3 Key Concepts: - An individual s characteristics are determines by factors that are passed from one parental generation to the next. - During gamete formation, the alleles for

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

Plasmid Isolation. Prepared by Latifa Aljebali Office: Building 5, 3 rd floor, 5T250

Plasmid Isolation. Prepared by Latifa Aljebali Office: Building 5, 3 rd floor, 5T250 Plasmid Isolation Prepared by Latifa Aljebali Office: Building 5, 3 rd floor, 5T250 Plasmid Plasmids are small, double strand, closed circular DNA molecules. Isolated from bacterial cells. Replicate independently

More information

QUESTIONSHEET 1. single stranded DNA by treatment with... This is then treated with

QUESTIONSHEET 1. single stranded DNA by treatment with... This is then treated with QUESTIONSHEET 1 Read through the following account of genetic engineering and then fill in the spaces with the most appropriate word or words. During the process of hormone manufacture by genetic engineering,

More information

Lecture 13. Molecular Cloning

Lecture 13. Molecular Cloning Lecture 13 Molecular Cloning Recombinant DNA technology depends on the ability to produce large numbers of identical DNA molecules (clones). Clones are typically generated by placing a DNA fragment of

More information

1. Which of the following correctly organizes genetic material from the broadest category to the most specific category?

1. Which of the following correctly organizes genetic material from the broadest category to the most specific category? DNA and Genetics 1. Which of the following correctly organizes genetic material from the broadest category to the most specific category? A. genome chromosome gene DNA molecule B. genome chromosome DNA

More information

Chapter 6: DNA: Hereditary Molecules of Life pg : DNA Replication and Repair pg

Chapter 6: DNA: Hereditary Molecules of Life pg : DNA Replication and Repair pg UNIT 3: Molecular Genetics Chapter 6: DNA: Hereditary Molecules of Life pg. 268-6.4: DNA Replication and Repair pg. 282-290 The DNA molecule is capable of replicating on its own. This is important for

More information

Chapter 20: Biotechnology

Chapter 20: Biotechnology Name Period Chapter 20: Biotechnology The AP Biology exam has reached into this chapter for essay questions on a regular basis over the past 15 years. Student responses show that biotechnology is a difficult

More information

Forensic DNA Testing Terminology

Forensic DNA Testing Terminology Forensic DNA Testing Terminology ABI 310 Genetic Analyzer a capillary electrophoresis instrument used by forensic DNA laboratories to separate short tandem repeat (STR) loci on the basis of their size.

More information

Thebiotutor.com A2 Biology OCR Unit F215: Control, genomes and environment Module 2.3 Genomes and gene technologies Notes & Questions

Thebiotutor.com A2 Biology OCR Unit F215: Control, genomes and environment Module 2.3 Genomes and gene technologies Notes & Questions Thebiotutor.com A2 Biology OCR Unit F215: Control, genomes and environment Module 2.3 Genomes and gene technologies Notes & Questions Andy Todd 1 Outline how the polymerase chain reaction (PCR) can be

More information

Vectors cont.. Pattern of Infection. Lytic cycle. Pattern of Infection. Question. Dr. Dinithi Peiris Dept. of Zoology

Vectors cont.. Pattern of Infection. Lytic cycle. Pattern of Infection. Question. Dr. Dinithi Peiris Dept. of Zoology Vectors cont.. Dr. Dinithi Peiris Dept. of Zoology 1 2 Pattern of Infection Lytic cycle 3 Pattern of Infection 4 Question What is the unique feature in this life cycle Phages causes lysis & cell death

More information

MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS

MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS Richard L. Hodinka, Ph.D. University of South Carolina School of Medicine Greenville Greenville Health System, Greenville, SC hodinka@greenvillemed.sc.edu

More information

Lecture 36: Basics of DNA Cloning-II

Lecture 36: Basics of DNA Cloning-II Lecture 36: Basics of DNA Cloning-II Note: Before starting this lecture students should have completed Lecture 35 Sequential steps involved in DNA cloning using plasmid DNA as vector: Molecular cloning

More information

Transfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.

Transfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells. Transfection Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide,

More information

Chapter 9 Homework Assignment

Chapter 9 Homework Assignment Chapter 9 Homework Assignment We will not cover the entire chapter. Please use the lecture notes and the Review Sheet for testable material I have decided to alter the homework assignment for Chapter 9.

More information

Structure and Function of DNA

Structure and Function of DNA Structure and Function of DNA DNA and RNA Structure DNA and RNA are nucleic acids. They consist of chemical units called nucleotides. The nucleotides are joined by a sugar-phosphate backbone. The four

More information

Biol 101 Exam 5: Molecular Genetics Fall 2008

Biol 101 Exam 5: Molecular Genetics Fall 2008 MULTIPLE CHOICE. This exam has 60 questions. All answers go on the SCANTRON provided. Choose the one alternative that best completes the statement or answers the question. 1) The genetic material of all

More information

Genetic Engineering and Biotechnology

Genetic Engineering and Biotechnology 1 So, what is biotechnology?? The use of living organisms to carry out defined chemical processes for industrial or commercial application. The office of Technology Assessment of the U.S. Congress defines

More information

INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE Q5B

INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE Q5B INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE ICH HARMONISED TRIPARTITE GUIDELINE QUALITY OF BIOTECHNOLOGICAL PRODUCTS: ANALYSIS

More information

Appendix D: Pre-lab Assignments and Gel Electrophoresis Worksheet

Appendix D: Pre-lab Assignments and Gel Electrophoresis Worksheet Appendix D: Pre-lab Assignments and Gel Electrophoresis Worksheet PCR Pre-Lab (pg. 1-3) PCR Pre-Lab Answers (pg. 4-7) RNAi Pre-Lab (pg. 8) RNAi Pre-Lab Answers (pg. 9-10 Gel Electrophoresis Worksheet (pg.

More information

Many cells will not take up plasmid during transformation Cells with plasmid can be identified because original plasmid contained gene for antibiotic

Many cells will not take up plasmid during transformation Cells with plasmid can be identified because original plasmid contained gene for antibiotic Many cells will not take up plasmid during transformation Cells with plasmid can be identified because original plasmid contained gene for antibiotic resistance (ampicillin) Use medium with ampicillin

More information

Bioinformatics: Network Analysis

Bioinformatics: Network Analysis Bioinformatics: Network Analysis Molecular Cell Biology: A Brief Review COMP 572 (BIOS 572 / BIOE 564) - Fall 2013 Luay Nakhleh, Rice University 1 The Tree of Life 2 Prokaryotic vs. Eukaryotic Cell Structure

More information

PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction Biological Sciences Initiative HHMI PCR Polymerase Chain Reaction PCR is an extremely powerful technique used to amplify any specific piece of DNA of interest. The DNA of interest is selectively amplified

More information

Section 12 3 RNA and Protein Synthesis

Section 12 3 RNA and Protein Synthesis Name Class Date Section 12 3 RNA and Protein Synthesis (pages 300 306) Key Concepts What are the three main types of RNA? What is transcription? What is translation? The Structure of RNA (page 300) 1.

More information

Chapter 15 Lecture Notes: Applications of Recombinant DNA Technology

Chapter 15 Lecture Notes: Applications of Recombinant DNA Technology Chapter 15 Lecture Notes: Applications of Recombinant DNA Technology I. In Vitro Mutagenesis: It is possible (and relatively easy) to make specific mutations in a gene using a variety of methods which

More information

How many of you have checked out the web site on protein-dna interactions?

How many of you have checked out the web site on protein-dna interactions? How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss

More information

Appendix 2 Molecular Biology Core Curriculum. Websites and Other Resources

Appendix 2 Molecular Biology Core Curriculum. Websites and Other Resources Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer http://www.insidecancer.org/ From the Dolan DNA Learning Center Cold

More information

Section 16.1 Producing DNA fragments

Section 16.1 Producing DNA fragments Section 16.1 Producing DNA fragments Recombinant DNA combined DNA of two different organisms The process of using DNA technology to make certain proteins is as follows: 1.) Isolation of the DNA fragments

More information

AP Biology Review Packet 4: Viruses, Bacteria and Expression & DNA Technology

AP Biology Review Packet 4: Viruses, Bacteria and Expression & DNA Technology AP Biology Review Packet 4: Viruses, Bacteria and Expression & DNA Technology 3A1- DNA, and in some cases RNA, is the primary source of heritable information. 3B1- Gene Regulation results in differential

More information

DNA replication. DNA RNA Protein

DNA replication. DNA RNA Protein DNA replication The central dogma of molecular biology transcription translation DNA RNA Protein replication Revers transcriptase The information stored by DNA: - protein structure - the regulation of

More information

Cloning vectors. E. coli Yeast Plants Insects

Cloning vectors. E. coli Yeast Plants Insects Cloning vectors E. coli Yeast Plants Insects Cloning vectors for E. coli The simplest cloning vectors are based on small bacterial plasmids Desirable properties: -Easy purification -High transformation

More information

4. DNA replication Pages: 979-984 Difficulty: 2 Ans: C Which one of the following statements about enzymes that interact with DNA is true?

4. DNA replication Pages: 979-984 Difficulty: 2 Ans: C Which one of the following statements about enzymes that interact with DNA is true? Chapter 25 DNA Metabolism Multiple Choice Questions 1. DNA replication Page: 977 Difficulty: 2 Ans: C The Meselson-Stahl experiment established that: A) DNA polymerase has a crucial role in DNA synthesis.

More information

Biochem 717 Gene Cloning. Prof Amer Jamil Dept of Biochemistry University of Agriculture Faisalabad

Biochem 717 Gene Cloning. Prof Amer Jamil Dept of Biochemistry University of Agriculture Faisalabad Biochem 717 Gene Cloning Prof Amer Jamil Dept of Biochemistry University of Agriculture Faisalabad How to construct a recombinant DNA molecule? DNA isolation Cutting of DNA molecule with the help of restriction

More information

Reminder. The genetic information in a gene is encoded in the sequence of bases on one strand of DNA.

Reminder. The genetic information in a gene is encoded in the sequence of bases on one strand of DNA. DNA Replication Genes are DNA. Reminder DNA is a double-stranded molecule. The genetic information in a gene is encoded in the sequence of bases on one strand of DNA. 1 10 20 30 40 50 60 70 80 90 100 AcatttgcttctgacacaactgtgttcactagcaactcaaacagacaccATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGC

More information

Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Chapter 9 Biotechnology and Recombinant DN Introduction to Biotechnology Recall from Chapter 8 that recombination, the reshuffling of genes between two DN molecules, forming recombinant DN, occurs naturally

More information

Genetic Engineering. Genetically-modified animals. Goals: Be able to. How did scientists get bacteria to produce BGH? What do you know about DNA?

Genetic Engineering. Genetically-modified animals. Goals: Be able to. How did scientists get bacteria to produce BGH? What do you know about DNA? Genetic Engineering Genetically-modified animals Bovine Growth Hormone (BGH) Protein that increases milk production when injected How did scientists get bacteria to produce BGH? Goals: Be able to Describe

More information

Basic Concepts Recombinant DNA Use with Chapter 13, Section 13.2

Basic Concepts Recombinant DNA Use with Chapter 13, Section 13.2 Name Date lass Master 19 Basic oncepts Recombinant DN Use with hapter, Section.2 Formation of Recombinant DN ut leavage Splicing opyright lencoe/mcraw-hill, a division of he Mcraw-Hill ompanies, Inc. Bacterial

More information

TECH NOTE Characterization of CRISPR/Cas9 introduced Mutations using the Guide it Indel Identification Kit

TECH NOTE Characterization of CRISPR/Cas9 introduced Mutations using the Guide it Indel Identification Kit TECH NOTE Characterization of CRISPR/Cas9 introduced Mutations using the Guide it Indel Identification Kit Streamlined method for characterizing the variety of indels introduced by genome editing technologies:

More information

Biotechnology. Chapter 20. Biology Eighth Edition Neil Campbell and Jane Reece. PowerPoint Lecture Presentations for

Biotechnology. Chapter 20. Biology Eighth Edition Neil Campbell and Jane Reece. PowerPoint Lecture Presentations for Chapter 20 Biotechnology PowerPoint Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Copyright

More information

Name Date Period. 2. When a molecule of double-stranded DNA undergoes replication, it results in

Name Date Period. 2. When a molecule of double-stranded DNA undergoes replication, it results in DNA, RNA, Protein Synthesis Keystone 1. During the process shown above, the two strands of one DNA molecule are unwound. Then, DNA polymerases add complementary nucleotides to each strand which results

More information

Ch. 12: DNA and RNA 12.1 DNA Chromosomes and DNA Replication

Ch. 12: DNA and RNA 12.1 DNA Chromosomes and DNA Replication Ch. 12: DNA and RNA 12.1 DNA A. To understand genetics, biologists had to learn the chemical makeup of the gene Genes are made of DNA DNA stores and transmits the genetic information from one generation

More information

CAP BIOINFORMATICS Su-Shing Chen CISE. 10/5/2005 Su-Shing Chen, CISE 1

CAP BIOINFORMATICS Su-Shing Chen CISE. 10/5/2005 Su-Shing Chen, CISE 1 CAP 5510-8 BIOINFORMATICS Su-Shing Chen CISE 10/5/2005 Su-Shing Chen, CISE 1 Genomic Mapping & Mapping Databases High resolution, genome-wide maps of DNA markers. Integrated maps, genome catalogs and comprehensive

More information

BNFO601: Introduction to Bioinformatics Protein Overexpression: Structure vs. Function

BNFO601: Introduction to Bioinformatics Protein Overexpression: Structure vs. Function BNFO601: Introduction to Bioinformatics Protein Overexpression: Structure vs. Function I. Overview of protein overexpression Take a look at that Pepsi (non-diet): high fructose corn syrup. Well, corn syrup

More information

I. Gene transfer and genetic engineering (Chapter 8) A. General concepts 1. Genetic information is contained in the nucleotide sequence of DNA

I. Gene transfer and genetic engineering (Chapter 8) A. General concepts 1. Genetic information is contained in the nucleotide sequence of DNA I. Gene transfer and genetic engineering (Chapter 8) A. General concepts 1. Genetic information is contained in the nucleotide sequence of DNA (sometimes RNA). Amino acids are specified by a triplet codon.

More information

Gene Mapping Techniques

Gene Mapping Techniques Gene Mapping Techniques OBJECTIVES By the end of this session the student should be able to: Define genetic linkage and recombinant frequency State how genetic distance may be estimated State how restriction

More information

How Do You Make A Transgenic Plant?

How Do You Make A Transgenic Plant? Home Page News Updates History of Plant Breeding What Are Transgenic Plants? How Do You Make A Transgenic Plant? Introduction to DNA Locating genes for plant traits Designing genes for insertion Transformation

More information

Expression and Purification of Recombinant Protein in bacteria and Yeast. Presented By: Puspa pandey, Mohit sachdeva & Ming yu

Expression and Purification of Recombinant Protein in bacteria and Yeast. Presented By: Puspa pandey, Mohit sachdeva & Ming yu Expression and Purification of Recombinant Protein in bacteria and Yeast Presented By: Puspa pandey, Mohit sachdeva & Ming yu DNA Vectors Molecular carriers which carry fragments of DNA into host cell.

More information

HCS604.03 Exercise 1 Dr. Jones Spring 2005. Recombinant DNA (Molecular Cloning) exercise:

HCS604.03 Exercise 1 Dr. Jones Spring 2005. Recombinant DNA (Molecular Cloning) exercise: HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA (Molecular Cloning) exercise: The purpose of this exercise is to learn techniques used to create recombinant DNA or clone genes. You will clone

More information

DNA is a double helix with two sugar phosphate backbones and nucleotide bases bridging the two chains.

DNA is a double helix with two sugar phosphate backbones and nucleotide bases bridging the two chains. BENG 100 Frontiers of Biomedical Engineering Professor Mark Saltzman Chapter 3 SUMMARY Nucleic acids are linear polymers made up of monomer units called nucleotides. Each nucleotide is composed of a pentose

More information

STANDARD 2 Students will demonstrate appropriate safety procedures and equipment use in the laboratory.

STANDARD 2 Students will demonstrate appropriate safety procedures and equipment use in the laboratory. BIOTECHNOLOGY Levels: 11-12 Units of Credit: 1.0 CIP Code: 51.1201 Prerequisite: Biology or Chemistry Skill Certificates: #708 COURSE DESCRIPTION is an exploratory course designed to create an awareness

More information

Chapter 6 DNA Replication

Chapter 6 DNA Replication Chapter 6 DNA Replication Each strand of the DNA double helix contains a sequence of nucleotides that is exactly complementary to the nucleotide sequence of its partner strand. Each strand can therefore

More information

Name Date Per. CH 15: GENETIC ENGINEERING STUDY QUESTIONS (pages )

Name Date Per. CH 15: GENETIC ENGINEERING STUDY QUESTIONS (pages ) WLHS / Biology / Oppelt Name Date Per CH 15: GENETIC ENGINEERING STUDY QUESTIONS (pages 416-433) 15.1: Selective Breeding 1) Define the following terms: Selective Breeding: Hybridization: Inbreeding: 2)

More information

Genetics Test Biology I

Genetics Test Biology I Genetics Test Biology I Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Avery s experiments showed that bacteria are transformed by a. RNA. c. proteins.

More information

DNA. Form and Function

DNA. Form and Function DNA Form and Function DNA: Structure and replication Understanding DNA replication and the resulting transmission of genetic information from cell to cell, and generation to generation lays the groundwork

More information

Compiled and/or written by Amy B. Vento and David R. Gillum

Compiled and/or written by Amy B. Vento and David R. Gillum Fact Sheet Describing Recombinant DNA and Elements Utilizing Recombinant DNA Such as Plasmids and Viral Vectors, and the Application of Recombinant DNA Techniques in Molecular Biology Compiled and/or written

More information

Lecture 37: Polymerase Chain Reaction

Lecture 37: Polymerase Chain Reaction Lecture 37: Polymerase Chain Reaction We have already studied basics of DNA/RNA structure and recombinant DNA technology in previous classes. Polymerase Chain Reaction (PCR) is another revolutionary method

More information

ECO-1.1: I can describe the processes that move carbon and nitrogen through ecosystems.

ECO-1.1: I can describe the processes that move carbon and nitrogen through ecosystems. Cycles of Matter ECO-1.1: I can describe the processes that move carbon and nitrogen through ecosystems. ECO-1.2: I can explain how carbon and nitrogen are stored in ecosystems. ECO-1.3: I can describe

More information

Biotechnology and Genomics

Biotechnology and Genomics Chapter14: pp. 249-263 BIOLOGY 10th Edition Biotechnology and Genomics Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. DNA probe array tagged DNA did bind to

More information