4 Safranin O/ fast green

Transcription

1 1030 Chinese Journal of Pathophysiology 2004,20 (6) : [ ] (2004) * 1, 2, 1, 1, 1 ( 1, 2, ) [ ] :(viartrils) (OA) OA : 24 OA, 12,( ) 2, 10,HE safranin O/ fast green, : OA,10 ( ) ( P < 0105),( P < 0105) :,OA [] ; ; [] R684 [] A (osteoarthritis,oa), 2 [1 ] OA, OA 250 mg 64 mg ),2, OA,(viartrils) OA,10, 3, 1 24,, 6-8, ( ) kg ;,, ;,;, 12, 3 % 30 mg/ kg, 1-2 cm,,,,, HE,, 4 Safranin O/ fast green,,, 4 d U/ d, [ ] [ ] * [ ] (No ) Tel : ; E - mail com Meeh - Rubner A = K W 2/ (viartrils,rottapharm Ltd1, 10, [2 ] : ;, 10 %,4 %8-12 d,,011 % fast green 011 % safranin O ( ), - [2 ] (histological - histochemical grading system, HHGS),

2 ,2-5,6-9,10 5 ABC(avidin - biotin complex) 10,,, ( Ki - 67,,,, ),4, ( (), lgg) -,DBA 10, 10, 500, 7, 3,, ( Ki67 = 2 4, 3, / 100) 3, Tab 1 Histological - histochemical grading of cartilage degeneration grade Grade Structure Normal 0 Surface irregularities 1 Pannus and surface irregularities 2 Clefts to transitional zone 3 Clefts to radial zone 4 Clefts to calcified zone 5 Complete disorganization 6 Cells Normal 0 Diffuse hypercellularity 1 Cloning 2 Hypocellularity 3 S/ Fstaining Normal 0 Slight reduction 1 Moderate reduction 2 Severe reduction 3 No dye noted 4 Total 0-13,,,,,, 2 HE safranin O/ fast green( S/ F) 4 (,,,),,, S/ F 4,,,,2,,, S/ F, 1 4,,,,, S/ F, 2 6,, HHGS, Student t,,spss ,,, 3 HHGS,, SPSS 1010,P <

3 ,Tamhane HHGS, P < Tab 3 The chondrocyte proliferative index between experimental and control group 2 Tab 2 The HHGS score of each group Cases Experimental group Control group P < 0105 vs control group. Case Control Control Experimental Experimental A * B * A * B * * A indicates the specimen of non - operational knee OA, * B in2 dicates operated specimen. HHGS :4, 7, 1 ;HHGS 10, 2, Mann - Whitney test P < 0105, 4 (3 4),,, 500 (3),, t, P < 0105,

4 1033, OA HHGS,,,, OA,,, [4 ],,,,,,, OA,,, OA,G 0 [5 ],,, mrna, A 2 (PLA 2 ), PKC [6 ],, () Ki - 67,OA ( P < 0105),,,,,,,,, OA 60 [ ], 1982,Vaz [7 ] [1 ] Felson DT, Zhang Y. An update on the epidemiology of knee OA and hip osteoarthritis with a view to prvention [J ]. Arthritis,OA Rheum,1998,41 (8) : ,, [2 ] Ayral X, Dougados M, Listrat V, et al. Chondroscopy. A new Reginster [8 ] method for scoring chondropathy[j ]. Semin Arthritis Rheum, , 22(5) : , [3 ] Van der Sluijs TA, Geesink RG, Van der Linden AJ, et al., The reliability of the Mankin score for osteoarthritis[j ]. J Or2 Reichelt [9 ] 155 OA, [4 ] Cloherty EK, Sultzman LA, Zottola RJ, et al. Net sugar trans2,lequesne,55 %33 % port is a multistep process. Evidence for cytosolic sugar binding Mealindon [10 ] sites in erythrocytes[j ]. Biochemistry, 1995,34(47) : Meta [5 ] Bassleer C, Henrotin Y, Franchimont P. In vitro evaluation of OA, drugs proposed as chondroprotective agents [J ]. Int J Tissue, React, 1992, 14 (5) : OA, [6 ] Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glu2 FDA, cosamine sulfate modulates dysregulated activities of human os2 OA teoarthritic chondrocytes in vitro[j ]. Osteoarthritis Cartilage,, 2000, 8 (3) : [11 ],10,,,, Ki - 67 thop Res, 1992, 10 (1) : [7 ] Vaz AL. Double - blind clinical evaluation of the relative effi2

5 1034 cacy of ibuprofen and glucosamine sulphate in the management of osteoarthrosis of the knee in outpatients[j ]. Curr Med Res Opin, 1982, 8 (3) : [8 ] Reginster JV, Deroisy R, Rovati LC, et al. Long - term ef2 fects of glucosamine sulfate on osteoarthritis progression : a randomized, placebo - controlled clinical trial [ J ]. Lancet, 2001,357 (27) : [9] Reichelt A, Forster KK, Fischer M, et al. Efficacy and safety of intramuscular glucosamine sulfate in osterarthritis of the knee[j ]. Arzneimittelforschung, 1994, 44 (1) : [10 ] McAlindon TE, LaValley MP, Gulin JP, et al. Glucosamine and chondrotin for treatment of osteoarthritis : a systematic quality assessment and meta - analysis[j ]. JAMA, 2000, 283 (11) : [11 ],,. [J ].,2001,5 (5) :64. Preventive effect of viartrils on the cartilage degeneration of osteoarthritis in rabbits ZHA Zhen - gang 1, YAO Ping 2, WU Hao 1, LIN Hong - sheng 1, LIU Ning 1 ( 1 Department of Orthopaedics, First Affiliated Hospital, 2 Department of Physiology, Medical College, Jinan University, Guangzhou , China) [ ABSTRACT] AIM : To determine whether the viartrils could provide a beneficial effect on the prevention of early/ middle stage osteoarthritis(oa) and affect the proliferation of chondrocytes. METHODS : An OA model was pro2 duced with severing the anterior, posterior cruciate ligaments of the knee in 24 adult New Zealand rabbits. The animals were then randomly divided into viartrils group and control group. After surgical operation, viartrils (mainly contains glu2 cosamine sulphate) 2 pills per day were administered to the animals in viartrils group. The animals were sacrificed and specimens were taken from the weight - bearing portion of the femoral condylar seven weeks after operation. Each case was evaluated according to a modified histological - histochemical grading system( HHGS) using HE and safranin O/ fast green staining slides, and immunohistochemical method was used to detect the proliferation of chondrocytes in articular cartilage. RESUL TS : The method of severing the anerior, posterior cruciate ligaments of the knee could successfully in2 duce the early/ middle stage model of OA. The pathological remark in control group was significantly higher than that in the viartrils group ( P < 0105). The proliferative index of viartrils group chondrocytes was also higher than that in control ( P < 0105). CONCL USION : These data indicate that viartrils could increase the proliferation of chondrocytes and pre2 vent the development of OA pathologic process. [ KEY WORDS] Osteoarthritis ; Viartrils ; Cartilage diseases