1 The Role of Quality Evaluation in Seed Production as Exemplified by Soybean Seed Production Francisco Carlos Krzyzanowski, Ph.D. Embrapa Soybean
2 SEED CERTIFICATION SYSTEM STANDARDS To reach them, several parameters should be followed such as: Genetic and Physical Purity Physiological Quality Seed Health All this three parameters require a very good Seed Testing Laboratory (Personnel and Equipment).
3 Genetic Purity It is the basis to reach high cultivar quality in seed production.
4 Soybean Breeder Seed Basic steps 1. Selection of typical plants of the required breeding line or cultivar in a field plot seeded for this purpose, known as: plant selection block PSB. 2. Usually for soybean the PSB is composed by 8 lines of 12 meters length.
5 Plant Selection Block PSB
6 Plant Selection Usually 400 to 500 plants are selected based on agronomic standard such as: plant height, cycle, leaf format, pubescence and pod colors at the time of maturity.
7 Each plant is threshed individually and its seeds are kept in individual bag. The seeds are evaluated for the following characteristics (genetic descriptor): seed colour, size and format, hilum colour, shape and peroxidase activity. Any atypical plant (seed bag) is discarded..
8 Seed colour
9 Seed size and format
10 Hilum colour
11 Hilum colour seeds from same soybean plant
12 Soybean Breeder Seed Basic steps 3. Progeny line Seeds coming from each selected plant gives origin to one Progeny Line. 40 seeds are sown in a single row of 3 meters length. A total of 300 or 400 lines spaced 0,5 meter wide is the first stage of breeder seed production. All plants are evaluated according to agronomic characteristics of line or cultivar.
13 Progeny line
14 Soybean Breeder Seed Basic steps 3. Progeny line Seeds coming from each selected plant gives origin to one Progeny Line. 40 seeds are sown in a single row of 3 meters length. A total of 300 or 400 lines spaced 0,5 meter wide is the first stage of breeder seed production. All plants are evaluated according to agronomic characteristics of line or cultivar. A line with any atypical plant is discarded.
15 Basic steps Soybean Breeder Seed 3. Progeny line Each approved line is harvested and threshed individually and seeds are evaluated again for genetic characteristics as previously described.
16 Soybean Breeder Seed Basic steps 3. Progeny block Each line gives origin to one Progeny block. In each block are sown 4 lines of 25 meters length spaced every 0,5 meter between them. The remaining seeds of each line are combined to form a bulk and are seeded around the area occupied by the blocks, for purpose of comparing the blocks for plant cycle, height due to soil effect (fertility).
17 Progeny Block
18 Soybean Breeder Seed Basic steps 3. Progeny block If there is some variation in the block for the traits such as: cycle, colour of flower and pubescence, plant height, leaf format, the whole block is discarded.
19 Soybean Breeder Seed Basic steps 3. Progeny block The homogeneous blocks which are considered identical are harvested and threshed individually, their seeds are again compared among the different blocks, based on morphological parameters (genetic descriptor).
20 Soybean Breeder Seed Basic steps 3. Progeny block / genetic seeds Genetic seeds are originated from joining of the homogeneous and identical progeny blocks.
21 Soybean Breeder Seed Peroxidase Activity Another resource to be used in conjunction with morphological traits is the Peroxidase Activity Test. The peroxidase enzyme occurs in most living tissues. It is widely distributed in the plant kingdom, being related to:
22 Soybean Breeder Seed Peroxidase Enzyme 1. Regulating the level of Indol Acetic Acid (IAA), 2. Mechanisms of resistance to diseases, 3. Regulation of membrane permeability, 4. Cell wall formation, 5. Seed dormancy when involved in controlling the entry of oxygen.
23 Soybean Breeder Seed Peroxidase Enzyme The action of peroxidase enzyme in the seed coat of soybean seed can divide the lines and cultivars into two groups with high activity, designated as positive reaction, and low activity, characterized as negative reaction.
24 Soybean Breeder Seed Peroxidase Test Test procedure For each line or cultivar, eight repetitions of one seed is used. Remove one slice of the seed coat, being careful not to let any fragment of the embryonic axis or cotyledons adhere to it, and place it in a test tube along with 10 drops of a 5% solution of Guaiacol.
25 Soybean Breeder Seed Peroxidase Test Test procedure After 10 minutes add one drop of hydrogen peroxide 40 volumes. After 30 to 40 seconds the colour appears or not, this being more evident after few minutes.
26 Soybean Breeder Seed Peroxidase Test Test procedure Cultivars with high peroxidase activity in the seed coat, present Reddish Brown Colour, designated as positive reaction. Cultivars of low activity showing no colour change characterize negative reaction.
27 Soybean Breeder Seed / Peroxidase Test Test procedure Seed coat removal
28 Soybean Breeder Seed / Peroxidase Test Test procedure Seed coat removal
29 Soybean Breeder Seed / Peroxidase Test Test procedure Seed coat removal
30 Soybean Breeder Seed / Peroxidase Test Test procedure Staining
31 Soybean Breeder Seed / Peroxidase Test Test procedure Staining - 10 drops of a 5% solution of Guaiacol.
32 Soybean Breeder Seed / Peroxidase Test Test procedure Staining - one drop of hydrogen peroxide 40 volumes.
33 Soybean Breeder Seed / Peroxidase Test Test procedure Staining - Reddish Brown Colour
34 Soybean Breeder Seed / Peroxidase Test Test procedure Staining - Reddish Brown Colour
35 Soybean Breeder Seed What could be another resource if there is uncertainty about the overall genetic purity of the block. It can still be analysed based on their genetic profile or DNA through the use of Molecular Markers.
37 Soybean Breeder Seed / Molecular Markers The evaluation of genetic purity of the soybean seeds based on DNA molecular markers is conclusive, since they do not suffer from environmental influences, and the data obtained are reproducible and stable.
38 Soybean Breeder Seed / Molecular Markers Microsatellite molecular markers are indicated for this type of analyses to be highly polymorphic in soybean, highly reproducible and codominant..
40 Soybean Breeder Seed Microsatellite molecular markers Satt 179 Satt 181
41 Physical Purity Genetic seed must be free of weed seeds. It means that the seed field has to be clean.
42 Physiological Quality Along with the germination test, the tetrazolium test is one of the most powerful tools to assist in identifying the levels of viability and vigour of soybean seed. The tetrazolium test provides a vigour index, and reveals the causes of seed weakness, such as mechanical damage, field and storage deterioration, stink bug damage, and damage to heat and frost.
43 WEATHERING DAMAGE
44 Determined by the exposure of seeds to high temperature associated with alternate wetting-drying cycles before harvest. rainfall relative humidity dew
47 Weathering Field deterioration due to seed moisture fluctuation. Hilum Rain Rain RH RH RH 15% MC 19% MC 14% MC RH 18% MC 13% MC
48 Weathering Field deterioration due to seed moisture fluctuation.
49 Consequences presence of wrinkles yield reduction higher susceptibility to mechanical damage reduction in germination and vigour
51 Deterioration due to weathering identified by the Tetrazolium test.
52 Evolution of Weathering Damage in Soybean Seed During Storage Danos por umidade na classe 3 (%) Weathering damage - TZ class 3 ( %) High Alto vigor vigour Y 33,9761 0, X R 2 0, 5622 Médio Medium Y 43,5595 0, X R 2 0, Storage period (days) Tempo de armazenamento (dias) CV - Embrapa 48 Source: Moreano (2009) - UEM
70 Clorox test for estimating mechanical damage Seed without apparent damage Seed with apparent damage
71 Clorox test for estimating mechanical damage Seed with apparent damage
72 Clorox test for estimating mechanical damage Repetition 1 Repetition 2
73 Clorox test for estimating mechanical damage Repetition 1 Repetition 2
74 Screen test for estimating mechanical damage
75 Screen test for estimating mechanical damage
76 clorox test (%) hipoclorito (%) Mechanical damage Screen test X Clorox test 60 Seven Soybean Crops 1998 a 2004 to SAFRAS - SOJA - COMPLETO colhedoras sementes quebradas (%) 1705 harvest broken seeds (%)
77 vigour vigor (%) Mechanical damage Screen test X Vigour 120 Seven Soybean Crops 1998 a 2004 to SAFRAS - SOJA - COMPLETO colhedoras harvest sementes quebradas (%) broken seeds (%)
78 vigour vigor (%) Mechanical Damage Clorox Test X Vigour 100 Seven Soybean Crops 1998 a to SAFRAS - SOJA - COMPLETO harvest 1720 colhedoras hipoclorito (%) clorox test (%)
79 Mechanical damage identified by tetrazolium test.
80 Mechanical damage identified by tetrazolium test.
81 Sanitary seed quality - Phomopsis
82 Sanitary seed quality - Coletotrichum
83 Sanitary seed quality - Fusarium
84 SEED TESTING LABORATORY THE HUB OF A SEED QUALITY CONTROL PROGRAM STORAGE PRE - SALE PROCESSING PRE SOWING STL DRYING PRE HARVEST HARVEST RECEPTION
85 INTERNACIONAL SEED TESTING ASSOCIATION - ISTA TECHNICAL COMMITTEES RULES FOR TESTING SEEDS LABORATORY ACCREDITATION AND PROFICIENCY TESTING SYSTEM WORKSHOPS ANNUAL MEETING ISTA CONGRESS SEED SCIENCE AND TECHNOLOGY SEED TESTING INTERNATIONAL ISTA provides the knowledge for quality evaluation in seed production.
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