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1 Biology 12 Name: Metabolism Practice Test Section 1: What is an enzyme? 1. Which of the following statements is true about enzymes? a) 3D shape can vary and still be active b) they may catalyze only 1 specific reaction c) boiling temps do not affect activity d) they are unaffected by changes in ph 2. Enzymes a) give energy to metabolic reactions b) speed up metabolic reactions c) change the direction of reactions d) act as a buffer in metabolic reactions 3. Enzymes are specific. This means that they a) have a preferred ph b) have a preferred temperature c) have a particular substrate d) are only in certain cells 4. Most enzymes a) keep the speed of the chemical reaction constant b) inhibit the speed of the chemical reaction c) speed up the chemical reaction d) have no effect on the speed of the chemical reaction 5. Most enzymes are a) lipids b) carbohydrates c) proteins d) nucleic acids 6. One enzyme a) allows a chemical reaction in a cell to occur at moderate temperatures b) raises the energy of activation of a reaction c) speeds up 2 or more reactions in a cell d) cannot be used after the reaction 7. Each enzyme has a particular substrate because enzymes a) increase the energy of activation b) decrease t

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3 Section 3: Factors That Affect the Rate of Enzyme Catalyzed Reactions 15. Initially, as the substrate concentration is increased, the reaction rate a) slows down b) speeds up c) stays the same d) speeds up then decreases 16. The graph shows the effect of ph on three different enzyme-catalyzed reactions. Which of the following would best describe the effect of ph on enzyme-catalyzed reactions? a) enzyme action increases as ph increases b) enzyme action decreases as ph increases c) enzymes work best in an acid environment d) each enzyme works best within a specific ph range 17. The diagram below represents the shapes of reacting molecules in a living system. When molecule X was added to the system, the amount of product decreased. Molecule X must be acting a) as a coenzyme. b) to denature the reactants. c) as a competitive inhibitor. d) to synthesize more of molecule W. 18. An experiment was performed to determine the effect of changing temperature on the speed enzyme activity. Substrate was placed in test tubes. The tubes were then placed in water baths of various temperatures. The substrate concentration in each tube was then measured. The results are graphed below. 10 C 40 C 50 C Give the amount of substrate in the tube at 10 C, 40 C and 50 C and explain why these substrate concentrations are different at each temperature.

4 19. Use the data table below Substrate Concentration Amount of Product Formed (milligrams/milliliter) (milligrams/milliliter) a) Which substrate concentration will initially yield the maximum amount of product formed? b) Explain why the amount of product formed does not increase as the substrate concentration goes beyond 30 mg/ml. 20. The following data show the rate of an enzyme catalyzed reaction at various temperatures. a. Graph the data on the grid provided. b. Use the graphed data to describe the effect of temperature on the rate of enzyme activity.

5 21. State the effect of the following on the rate of digestion of starch by amylase. a. Warming enzyme from 20 C to 37 C. b. Changing the ph of the enzymes from 7 to 2 c. Adding a heavy metal to the enzyme 22. An experiment was carried out to measure the effect of ph on enzyme activity. The following steps were performed: 1. An equal volume of hydrogen peroxide was added to eight numbered test tubes at 20 C. 2. The contents of each test tube were maintained at a different ph. 3. An equal mass of liver was added to each test tube. Liver, which contains the enzyme catalase, catalyzes the following reaction: Hydrogen peroxide oxygen gas (O2) and water The time to collect 10 ml of oxygen gas (O2) from each tube was measured and recorded in the table below. a) Draw a graph that compares the time taken to collect 10 ml of oxygen gas (O2) produced to the ph of the solution. Label the x-axis as ph of solution. (Use a pencil to graph the data) b) What appears to be the optimum ph for the enzyme catalase? c) Using your graph, estimate the time it takes to collect 10 ml of oxygen gas (O2) at ph 6.5. d) Explain what causes the results observed between ph 8 and ph 12

6 23. The graph shows the change in the rate of an enzyme-catalyzed reaction over time. a) Explain why the rate became constant at time X. b) Which labeled line correctly illustrates what would occur if more enzyme was added at time Y. Explain your answer. Section 4: Metabolism and Metabolic Pathways 24. If represents a metabolic pathway, then letter E would be a) a substrate b) a product c) an end product d) an enzyme 25. In a metabolic pathway a) product of 1 reaction becomes substrate of next reaction b) ATP is always released c) the same enzyme is used for all reactions d) the end product is never formed 26. Which of the following statements is false? a) metabolism is the sum of all chemical activities inside a cell b) a metabolic pathway involves only one chemical reaction c) one metabolic pathway may lead into another metabolic pathway d) the reactants in an enzymatic reaction are called substrates 27. An experiment investigating enzyme activity was carried out. A test tube was prepared containing substrate solution W and enzyme solutions 1 and 2. The reactions that occur in the test tube are summarized below: The letters represent substrates and products and the numbers represent enzymes. a. State two ways to increase the rate of production of product Y. b. A substance is added to the test tube containing substrate W. As a result, no product is formed. Suggest what this substance may be and explain how it achieves these results.

7 28. An experiment investigating enzyme activity is carried out. A test tube is prepared containing substrate solution W and enzyme solutions 1, 2 and 3. The reactions that occur in the test tube are summarized to the right. a) Describe two ways in which the rate of production of product Y can be increased. b) A substance is added to the test tube. As a result, no product Y is formed, but product Z is still formed. Explain why. Section 5: Cellular Respiration, ATP and Thyroxin 29. ATP is the energy currency of cells because it a) is a large molecule b) carries a positive charge c) contains high energy phosphate bonds d) is made from glucose 30. What is the common energy currency in cells for the synthesis of molecules? a) glucose b) proteins c) lipids d) ATP 31. ATP contains a) 1 phosphate b) 2 phosphates c) 3 phosphates d) no phosphates 32. The high energy bond in ATP is found in or between a) adenine base b) cytosine base c) adenosine d) the phosphate groups 33. When ATP is broken down a) ADP is formed b) ADP + phosphate is formed c) ADP + phosphate + energy is formed d) Lactic acid is formed 34. Which of these statements about ATP is false? a) ATP contains 3 phosphate groups b) ATP is used for muscular contractions c) ATP is not used in plants d) ATP contains high energy phosphate bonds 35. ATP is the energy currency of cells because it a) is easily oxidized b) contains high energy phosphate bonds c) combines with other substrates d) can be synthesized from inorganic components

8 36. The equation ADP + P ATP is energy a) requiring b) releasing 37. Give one hormonal response in the body to lower than normal body temperature over a prolonged period of time and explain why this response occurs. 38. The diagram below represents a molecule found in all living cells. a) What is this molecule called? b) What occurs if one phosphate is removed? Why? c) Name one cellular function that requires the above molecule. Section 6: Higher Level Thinking Question (application of knowledge) 39. The cells of the thyroid gland are able to take in iodine atoms and may contain iodine concentrations up to 25 times that of the surrounding tissue fluid. In an experiment designed to study factors affecting the rate of iodine intake, thyroid cells were cultured and placed in a medium containing normal blood concentrations of iodine. Temperature and glucose concentrations were varied and the effects recorded. The results of the study are shown in the table below. a) Explain the observed results for sample A. b) Explain the observed results for sample B. c) Explain how the movement of amino acids into the cells would be affected by the conditions in sample B

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