PAA Cell Culture and More. Quality Control

Transcription

1 Plastic Labware

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3 PAA and More PAA is specialized in the manufacture and worldwide distribution of cell culture products for research, development, diagnostic and biopharmaceutical production. For more than 20 years we accompany our customers in their daily laboratory routine and provide them with high quality animal and human sera, media, reagents and buffer solutions. Our products are manufactured in three facilities based in Austria, Australia and Canada. In parallel with our investment in facilities and capabilities we have also committed considerable funds into new product development to satisfy the requests of our research based customers. We have expanded our product range with new and innovative media and several new reagents. Beside the excellent product quality, our main aim is to provide outstanding customer service. In consequence of this we expanded our product portfolio with disposables such as plastic labware to enable our customers to purchase their laboratory equipment conveniently from one source. The plastic labware is designed by leading manufacturers, using high precision injection molding, polymer technology and surface chemistry. Quality characteristics such as the absence of endotoxins, non-cytotoxicity as well as exceptional optical clearance and good cell performance are the basic principles of our cell culture products. For the area of cell imaging we provide highly innovative products for cell based assays, fluorescence microscopy and high content screening. They feature high plate planarity, low background interference and high light transmission for challenging experiments. Our company is known for its focus on high throughput laboratories as well as the support of smaller research groups. Therefore, we offer our new product line in different purchase units to satisfy the individual needs of our customers. Quality Control The basic requirement for the reproducibility and comparability for cell and tissue culture in the field of in vitro research is first class quality standard. Sterility Sterilization is a process in which surfaces of materials and objects are freed from living organisms. As a 100 % security can not be guaranteed, a reduction is required, so that a SAL-value (Sterility Assurance Level) of 10-6 is reached. This means that in a million equally treated units of the sterilized products only one augmentable micro-organism should be included. This SALvalue can be achieved by gamma irradiation, beta irradiation and ethylene oxide sterilization. RNA/RNases and DNA/DNases DNases and RNases belong to a group of enzymes that destroy nucleic acids. They protect living organisms against the invasion of foreign DNA and foreign RNA by hydrolyzing the phosphodiester bond between the 5'-phosphate group of a nucleotide and the 3'-hydroxyl group nearby. DNA/DNases and RNA/RNases have a great potential for transmission so that protection during working processes is necessary. Pyrogens Pyrogens are high molecular weight substances of polymerous structure which might effect cell growth and proliferation. They can either be an endotoxin or an exotoxin. Endotoxins are natural compounds of the cell wall of gram-negative bacteria and potentially toxic. The biological activity of endotoxin is associated with the lipopolysaccharides (LPS) of the bacterial membrane. As pyrogens are heat stable and cannot be destroyed by gamma irradiation it is necessary to test for their absence. The Limulus Amoebocyte Lysate Assay (LAL assay) is a very sensitive method for the detection of the presence and quantity of bacterial endotoxins. The LAL test complies with the FDA guidelines. Cytotoxicity Cytotoxicity describes the ability of distinct chemical substances to damage cells or tissues. Especially for reliable toxicological studies in cell culture research the quality of culture vessels is of high relevance. It must be guaranteed that they are free of cytotoxic substances to prevent false positive results. The cytotoxicity is determined by using mammalian cells according to EN ISO Materials Most of our plastic labware is made of either polystyrene or polypropylene. Polystyrene (PS) is a high quality polymer with an excellent optical clarity. This feature facilitates the optical control of cells cultivated in PS flasks, wells and dishes. PS is also applicable for the manu facturing of serological pipettes and tubes where precise mea-surements are necessary. Polypropylene (PP) is a linear hydrocarbon polymer used for many different applications. PP is characterized by a high chemical resistance to many chemical solvents, bases and acids. It shows thermal stability in a temperature range of 80 C to +121 C and is therefore autoclavable. Due to its high mechanical resistance PP is used for products which are exposed to pressure occurring e.g. at high speed centrifugation. Surface Treatment Our plastic labware used for the cultivation of adherent cells is plasma treated. This surface technology is based on the use of an ionized gas at a certain pressure to increase the surface energy. As a result the polystyrene surface of the cell culture vessels is more hydrophilic and allows optimal cell attachment. PAA The Company 3

4 Table of Contents Flasks Dishes Cell Scrapers Cell Lifter Multiwell Tissue Culture Plates Well Microplates for Colorimetric Assays Plates with Inserts C-Chip Neubauer Improved / Malassez Tray Plate Square Dish Center Well Culture Dish Confocal Dishes Coverslips Serological Pipettes Petri Dishes Blood Separation Tubes Conical Centrifuge Tubes Position Tubes Test Tubes Microtubes PCR Tubes Pipette Tips Filter Pipette Tips Dispenser / Dispenser Tips Multiwell Plates Tray Plate Square Dishes Erlenmeyer Flasks Loops & Needles Spreader PAA The Company

5 96 Well (ELISA Plates) Strip Black & White Well Cell Imaging Imaging Plates CG, with Glass Bottom Imaging Plates FC, with Fluorocarbon Film Bottom. 42 Imaging Dishes CG (Cover Glass) Imaging Dish Stage Frames Imaging Chambers / Imaging Chambers CG Imaging Chamber Accessories Slides Slide Tray Plates CytoCapture Handling & Storage Cryotubes Inserts for Cryotubes Working Rack for Cryotubes Cryo Boxes Storage Boxes Storage Boxes with Hinged Cover Conical Centrifuge Tube Racks Microtube Racks PCR Tube Racks Reservoirs Square Bottles Autoclave Bags Appendix Alphabetical Index Material Properties Chemical Resistance of Various Materials PAA The Company 5

6 Cell culture is a technique which enables the cultivation of higher organisms. It can serve as a simplified model to understand the regulation and the biochemistry of eukaryotic cells. Therefore, PAA offers high quality consumables for performing different kinds of cell culture procedures. Quality characteristics such as the absence of endotoxins, non-cytotoxicity as well as excellent optical clearance and good cell performance are the basic principles of our products. 6 PAA The Company

7 Flasks Dishes Cell Scrapers Cell Lifter Multiwell Tissue Culture Plates Well Microplates for Colorimetric Assays Plates with Inserts C-Chip Neubauer Improved / Malassez Tray Plate Square Dish Center Well Culture Dish Confocal Dishes Coverslips PAA The Company 7

8 Flasks In research cell culture flasks are routinely used for the cultivation of eukaryotic cells. They are first class products for adherent cells as well as for suspension cultures and applicable for different requirements. PAA offers standard and filter cap cell culture flasks of excellent optical clarity. The filter caps are equipped with a high quality membrane with a pore size of 0.22 µm allowing consistent gas exchange. Cell culture flasks with green caps are suitable for adherent cells, providing a surface treated zone for optimal cell adhesion and growth. Flasks with white caps meet the requirements of suspension cells. The design of the flasks provides flexibility in media volume and saves incubator space. In addition, it facilitates the accessibility with cell scrapers and serological pipettes in an optimal way. The cell culture flasks are easy to stack and stand firmly in the incubator. The angled neck of the high profile flask reduces the risk of wetting the inside of the cap with medium and thus provides additional protection against contamination. Cat. No. Material Cap Type Cap Colour Growth Area (cm 2 ) > With standard caps or filter caps available > For adherent and suspension cells available > Plasma treated for adherent cells (flasks with green cap) > Sterilized > Angled, wide neck for easy access > Volume graduations on both sides > Large field of inscription for clear identification > Non-pyrogenic > Non-cytotoxic Working Volume (ml) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA70025X PS Filter Green PAA70075X PS Filter Green PAA71175X PS Filter Green PAA70125X PS Standard Green PAA70175X PS Standard Green PAA72175X PS Standard Green PAA70325X PS Filter White PAA70375X PS Filter White PAA74175X PS Filter White PAA70225X PS Standard White PAA70275X PS Standard White PAA73175X PS Standard White PAA The Company

9 Dishes Cell culture dishes are applicable as alternative to cell culture flasks and appropriate for different requirements. PAA offers cell culture dishes of excellent optical clarity. High quality surface treatment of the growth area enables constant results. Easy stackability ensures a firm stand in the incubator. A raised ring around the circumference of the dish provides a secure grip of dish and lid. This reduces the risk of contamination due to unintentional removal of the lid. Cams inside the lid allow constant ventilation in a closed system. Cat. No. Material External Dimension (mm) Internal Dimension (mm) Growth Area (cm 2 ) > Available in four different sizes (Ø 35, 60, 100, 150 mm) > Easy grip for simplified aseptic handling > Optically clear for microscopy > Optimal gas exchange due to ventilation cams in the lid > Plasma treated for optimal growth conditions > Sterilized > For adherent and suspension cells > Safe and stable storage due to raised stacking rings > Non-pyrogenic > Non-cytotoxic Working Volume (ml) External Grip Surface Treated For a successful cell culture... Sterilized Pieces / Bag Purchase Unit (pcs) PAA20035X PS 35 x x PAA20060X PS 60 x x PAA20101X PS 100 x x PAA20151X PS 150 x x the use of high quality products is inevitable. PAA is a reliable partner, offering a broad range of animal and human sera, standard and cell type specific media, buffer solutions and reagents such as antibiotics, detachment solutions, growth supplements and others. Our online catalogue provides you with detailed information at: PAA The Company 9

10 Cell Scrapers & Cell Lifter Cell scrapers and lifters allow an efficient and gentle removal of adherent cells from the cultivation surface. They can be used as an alternative or in addition to enzymatic detachment solutions. The cell scrapers are designed for optimal contact to the culture vessel and appropriate to flasks, dishes and plates. Even in culture containers with poorly accessible surfaces the free rotatability of the blade enables easy and complete scraping of attached cells. The thin and flexible blades of the scrapers are made of polyethylene, a material that does not affect the cells. The cell lifter is especially suitable for gentle harvesting of cells and tissues from dishes and plates. The one piece design with a small and a big blade on one handle facilitates working with diverse culture vessels. Cell Scrapers Cat. No. Material (handle) Cell Lifter Material (blade) > Adjustable blade for optimal flexibility (cell scraper) > Two blades for several well formats (cell lifter) > Sterilized > Non-pyrogenic > Single packaged Blade Width (cm) Total Length (cm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA90020X PP PE PAA90030X PP PE PAA90040X PP PP 1.2 / PAA The Company

11 Multiwell Tissue Culture Plates Multiwell tissue culture plates are used in all areas of cell culture and molecular biology. PAA provides multiwell plates in several sizes and for different applications. All of them are cell culture tested and designed for reduced cross contamination risk. The arrangement of each well standing on its own and corresponding condensation rings on the inner side of the lid allows safer sample handling. Thus the risk of contamination from sample material being carried over is minimized. An alphanumeric well coding ensures easy sample identification. A plasma treated or non-treated version can be selected for each multiwell plate (except 4 well plate) depending on the experiments. Cat. No. 1 1 U-bottom > Round bottom shape > No sharp corners for easy pipetting and cleaning > Suitable for +/ analyses Well Format 2 Material 2 F-bottom > Flat bottom shape > For precise optical measurements > For microscopic applications External Dimension (mm) Well Dimension Ø (mm) > Optically clear for microscopy > Lid with condensation rings (except PAA30004, PAA30004X) > Ergonomic grip for simplified aseptic handling > Optimal gas exchange due to ventilation cams in the lid > Stackable and space saving design > For adherent and suspension cells > Sterilized > Non-pyrogenic > 96 well with U- or F-bottom Growth Area (cm²) Working Volume (ml) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA F PS 66.0 x PAA30004X 4 F PS 66.0 x PAA30006X 6 F PS 85.4 x PAA30012X 12 F PS 85.4 x PAA30024X 24 F PS 85.4 x PAA30048X 48 F PS 85.4 x PAA30096X 96 F PS 85.4 x PAA34096X 96 U PS 85.4 x PAA32006X 6 F PS 85.4 x PAA32012X 12 F PS 85.4 x PAA32024X 24 F PS 85.4 x PAA32048X 48 F PS 85.4 x PAA32096X 96 F PS 85.4 x PAA34296X 96 U PS 85.4 x PAA The Company 11

12 96 Well Microplates for Colorimetric Assays Colorimetric assays, e.g. fluorescence or luminescence measurements are more and more common in cell culture research. To avoid negative side effects such as background interference, autofluorescence or cross talk the use of black or white pigmented microplates is recommended. PAA provides two different high quality 96 well microplates, suitable also for various high throughput assays. High signal-tonoise ratio with low background is a characteristic feature of these plates. The 96 well microplates are cell culture tested and designed for reduced cross contamination risk. The arrangement of each well standing on its own and corresponding condensation rings on the inner side of the lid allow safe sample handling. Thus the risk of contamination from sample material being carried over is minimized. An alphanumeric well coding ensures easy sample identification. Cat. No. Material > Available in white and black > High signal-to-noise ratio > Low background interference > Plasma treated for optimal growth conditions > Non-transparent flat bottom > Sterilized > Single packaged Plate / Lid Color External Dimension (mm) Well Dimension Ø (mm) Growth Area (cm²) Total Vol. / Well (µl) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA30196 PS White / Trans x PAA30196X PS White / Trans x PAA30296 PS Black / Black 85.4 x PAA30296X PS Black / Black 85.4 x PAA The Company

13 Plates with Inserts Cell culture inserts are used in a variety of applications including invasion, migration, transport, drug uptake, cellular polarization, chemotaxis, co-culture experiments and toxicity studies. PAA offers cell culture inserts with PC or PET membrane that closely mimic an in vivo environment, resulting in improved cell attachment, growth and differentiation of various cell types. The special manufacturing process of the membrane ensures a pre- cise pore diameter and a consistent pore size in experiments. The inserts are available with pores of 0.4 µm, 3.0 µm and 8.0 µm diameter. Due to their transparency and thinness the inserts with PET membrane are suitable for microscopic works. The PC membrane features excellent staining, inertness and ensures low background interference. Cat. No. Membrane Material Membrane Pore Size (µm) > Convenient usage > Consistent pore size > Compatible with automated systems > Packaged with multiwell plate (one plate per bag) > Distance from membrane to the bottom of the well: mm > Nominal membrane thickness: 7 10 µm > Pore Size: 0.4, 3.0 or 8.0 μm > Membrane material: PC or PET > Sterilized Membrane Diameter (mm) Growth Area (cm 2 ) Working Volume (ml) Sterilized Well Format Inserts / Plate Purchase Unit (plates) PAA35006X PC well 6 4 PAA35106X PC well 6 4 PAA35206X PC well 6 4 PAA35024X PC well 12 4 PAA35124X PC well 12 4 PAA35224X PC well 12 4 PAA37006X PET well 6 4 PAA37106X PET well 6 4 PAA37206X PET well 6 4 PAA37024X PET well 12 4 PAA37124X PET well 12 4 PAA37224X PET well 12 4 PAA The Company 13

14 C-Chip Neubauer Improved / Malassez Hemocytometers are used in daily routine in cell culture, microbiology and haematology. The preparation of traditional glass chambers is a time-consuming and not reliably reproducible process that often ends with broken cover slips. PAA offers with the C-Chip Neubauer Improved and Malassez two disposable plastic hemocytometers for manual cell counting. They are very easy to use and the precise volume control leads to highly reproducible results. Two counting chambers are fitted with a clear grid pattern rich in contrast. The productivity is increased by eliminating cleaning steps. Redundancy of the cleaning step also reduces the risk of exposure to potentially infectious material. Neubauer Improved Cat. No. Grid Malassez Chambers per Slide > Easy to use > Precise quantification > High reproducibility > No need for cover slips > No need for cleaning and reuse > Time and cost saving > Quartz grade optical plastic > Clear grid pattern > Very light and nearly unbreakable > Single packaged Chamber Depth Chamber Volume Dimensions (mm) Pieces / Bag Purchase Unit (pcs) PAA404040X Malassez µm 20 µl 25 x 75 x PAA505050X Neubauer Improved µm 10 µl 25 x 75 x PAA The Company

15 Tray Plate Tray plates are highly suitable for multiple cell culture applications. PAA provides a tray plate that can be used for mammalian cell culture. The rectangular shape of the tray dish provides a larger surface area compared to round type dishes and easy handling for any application. The external dimension of this single well plate is identical to standard multiwell plates. > Optically clear > High plate planarity > Surface treated for tissue culture > Sterilized > Single packaged Cat. No. Square Dish Material External Dimension (mm) Square dishes are commonly used in cell culture applications, especially for mammalian cell culture. PAA offers a tissue culture treated square dish made of optically clear polystyrene. It is designed and tested for standard HTS systems for automated Internal Dimension (mm) Growth Area (cm 2 ) Surface Treated colony picking. The large surface area and the gridded bottom are useful for tracing of individual cell colonies. The grid consists of thirty-six squares with alphanumerical coding. > Large surface area > Optically clear > High plate planarity > Available with grids for determination of plating efficiency > Surface treated for tissue culture > Alphanumerical coding > Sterilized Sterilized Pieces / Bag PAA30001X PS x 85.6 x x 73.5 x Cat. No. Material External Dimension (mm) Internal Dimension (mm) Growth Area (cm 2 ) Surface Treated Sterilized Pieces / Bag PAA10125X PS 125 x 125 x x x Purchase Unit (pcs) Purchase Unit (pcs) PAA The Company 15

16 Center Well Culture Dish For the research of in vitro fertilization on animal cells PAA offers a convenient center well culture dish. The 60 mm x 15 mm dish features a 20 mm center well for oocyte culture and insemination, surrounded by a larger moat for humidification. The center well holds 3 ml of medium while the outer well holds 10 ml. The center well culture dishes are made of optical grade polystyrene for clear visualization by microscope. The easy grip provides convenient and secure handling. The dishes are tissue culture treated and sterile. Cat. No. Confocal Dishes Material External Dimension (mm) The in vitro cultivation of cells under laboratory conditions is common practice for a huge variety of applications. The range of cell types is almost limitless, e.g. plant and insect cells as well as avian and mammalian cells. PAA offers confocal dishes made of polystyrene with coverglass bottom or removable coverglass insert. The dishes facilitate preparation of cells for microscopic analyses. They are suitable > For research only > Optical grade polystyrene > Easy grip for secure handling > Surface treated for tissue culture > Sterilized > Non-pyrogenic > Non-cytotoxic Internal Dimension (mm) Surface Treated Sterilized Pieces / Bag for use in high resolution and inverted microscopy, fluorescence imaging in living cells, confocal microscopy, phase contrast microscopy and micro-manipulation or anywhere where highest image quality is desired. A raised ring around the circumference of the dish provides a secure grip of dish and lid, reducing the risk of contamination due to unintentional removal of the lid. > Facilitated preparation of cells for microscopic analyses > Optically clear with low background fluorescence > Easy grip for secure handling > Sterilized > Hole size Ø: 13 mm > Coverglass size: 22 x 22 mm > Coverglass thickness: 0.13 ~ 0.16 mm Purchase Unit (pcs) PAA20260X PS 60 x Bottom Type Cat. No. Material Insert Type External Dimension (mm) Type Growth Area (cm 2 ) Working Volume (ml) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA PS 35 x 10 Coverglass bottom PAA100350X PS 35 x 10 Coverglass bottom PAA PS 35 x 10 Coverglass bottom PAA200350X PS 35 x 10 Coverglass bottom PAA PS 35 x 10 Coverglass insert PAA100351X PS 35 x 10 Coverglass insert PAA The Company

17 Coverslips PAA offers disposable coverslips made of Aclar, suitable for staining cultured cells and high magnification microscopy. Aclar is a polychlorotrifluoroethylene (PCTFE) material useful for both tissue culture and electron microscopic purposes. It is crystal clear and features excellent chemical resistance. In contrast to glass, Aclar coverslips are UV light transmissive and resistant to breakage. The material provides easy cell attachment and growth. > Great microscopic properties > Resistant to breakage > Excellent chemical resistance > Optimal cell attachment and growth > Facilitates staining and fixation of cells > 110 µm thickness > Non autoclavable > Sterilized Cat. No. Material Dimension (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA20009X Aclar 9 x PAA20012X Aclar 12 x PAA20018X Aclar 18 x PAA20025X Aclar 25 x Aclar is a trademark of Honeywell International Inc. AccuMax the basis for accurate cell counting FACS analysis (Fluorescence Activated Cell Sorting) is a specialized form of flow cytometry, where cells can be sorted one cell at a time. A major problem in eukaryotic cell culture is the clump formation of cells. AccuMax is a ready to use enzymatic dissociation solution containing proteolytic, collagenolytic and DNase enzymes. The raw material is derived from crustaceans and hence a potential contamination risk is reduced to a minimum. Read more about AccuMax at AccuMax is a trademark of ICT PAA The Company 17

18 Molecular biology is the study of biological activity on a molecular level. It includes the interaction between DNA, RNA and proteins and its regulation. PAA provides plastic consumables such as microtubes, pipette tips and serological pipettes that are essential for many different applications. 18 PAA The Company

19 Serological Pipettes Petri Dishes Blood Separation Tubes Conical Centrifuge Tubes Position Tubes Test Tubes Microtubes PCR Tubes Pipette Tips Filter Pipette Tips Dispenser / Dispenser Tips Multiwell Plates Tray Plate Square Dishes Erlenmeyer Flasks Loops & Needles Spreader PAA The Company 19

20 Serological Pipettes Serological pipettes are an essential tool for precise volume measurement. In contrast to glass, plastic pipettes provide the advantage of constant sterility due to individual packaging. PAA offers disposable serological pipettes that are single wrapped in plastic/paper packaging with peel-off function for aseptic pipette removal. The serological pipettes are also availa- ble in bulk packages, both sterile and non-sterile. High grade polystyrene provides maximum transparency. To avoid suction of liquid into the pipetting device all pipettes are supplied with a cotton plug. A color code, following international standards, facilitates the identifi cation of the pipette size. The slim pipette tip and the small diameter allow outstanding accessibility. > High optical clarity > Maximum accuracy > Drop free pipetting > Color coded label (international code) > Increased volume from negative graduations > Black colored graduation > Sterilized > Non-pyrogenic > Single packaged Cat. No. Volume (ml) Color Code Material Sterilized Pieces / Bag Purchase Unit (pcs) PAA92001X 1 Yellow PS PAA92002X 2 Green PS PAA92005X 5 Blue PS PAA92010X 10 Orange PS PAA92025X 25 Red PS PAA92050X 50 Violet PS PAA93005X 5 Blue PS PAA93010X 10 Orange PS PAA93025X 25 Red PS PAA94005X 5 Blue PS PAA94010X 10 Orange PS PAA94025X 25 Red PS PAA The Company

21 Petri Dishes PAA provides a large range (35 mm to 150 mm) of petri dishes for various laboratory purposes. They are primarily useful to grow bacteria, fungi and other microorganisms, but also applicable for seed germination and small animal observations. In addition, they are suitable for other day-to-day laboratory practices such as storing samples. All of the petri dishes are produced with optically clear high quality polymers. Easy stackability ensures a firm stand. A raised ring around the circumference of the dish provides a secure grip of dish and lid, reducing the risk of contamination due to unintentional removal of the lid. Cams inside the lid allow constant ventilation in a closed system. For special applications PAA offers a Bl-plate with a divided area for handling two types of microbes and a gridded bottom dish that facilitates the counting of colonies. Furthermore, a contact plate is available that is designed to allow a raised convex surface, when filled with agar, for the detection and counting of microbes from environmental surfaces. > Optically clear for microscopy > Optimal gas exchange due to ventilation cams in the lid > Easy grip for simplified aseptic handling (PAA10035X, PAA10060X, PAA10101X) > Divided area for handling two types of microbes (PAA10092X) > Easy detection and counting with the contact plate (PAA10061X) > Safe and stable storage due to raised stacking rings > Sterilized > User-friendly packaging Cat. No. Material External Dimension (mm) Internal Dimension (mm) Growth Area (cm 2 ) External Grip Sterilized Pieces / Bag Purchase Unit (pcs) PAA10035X PS 35 x x PAA10060X PS 60 x x PAA10061X PS 60 x 15 (Contact plate) PAA10090X PS 90 x x PAA10091X PS 90 x x PAA10092X PS 90 x 15 (BI-plate) PAA10095X PS 90 x 15 (Grid) 85.2 x PAA10100X PS 100 x x PAA10101X PS 100 x x PAA10150X PS 150 x x PAA10151X PS 150 x x Dishes for cell culture purposes can be found on page 9. PAA The Company 21

22 Blood Separation Tubes The isolation of cells or subcellular particles is the first step for diagnostics or investigations in cell functionality or gene expression. The enrichment of cells from complex biological mixtures such as blood and other body fluids requires the use of special separation media. The blood sample has to be poured carefully over the separation medium in a time consumptive and laborious procedure. To simplify this process PAA provides a centrifuge tube with an incorporated porous barrier. This feature prevents a mixture of the sample material with the separation medium and a recontamination of the enriched cell fraction with unwanted blood components after centrifugation. > Simplified filling > Rapid separation > Precise and easy harvesting > No recontamination with unwanted blood components > Centrifugation up to 840 x g > Sterilized > Material of barrier: PE Cat. No. Volume (ml) Material Tube / Cap Separation of lymphocytes easily done with LSM 1077 External Dimension (mm) For the separation of lymphocytes or other cells out of blood special separation media are required. The media are used for the production of density gradients for the separation of blood components, which sediment during centrifugation due to gravity. PAA offers the ready to use separation solution LSM 1077 based on ficoll with a density of g/ml. Optimal osmotic conditions and a physiological ph level assure a separation without any loss of viability of the cells. For more information visit Sterilized Pieces / Bag Purchase Unit (pcs) PAA PP / HDPE 30 x PAA535710X 50 PP / HDPE 30 x PAA The Company

23 Conical Centrifuge Tubes Conical centrifuge tubes are used for different applications in laboratories. PAA provides high strength and non toxic conical tubes in three different sizes to meet the various demands of scientists. They are made of polypropylene or polystyrene and sterile packaged in bags or already sorted in racks. The conical tubes withstand centrifugal forces up to 3000 x g respectively 9000 x g. For high speed centrifugation a specially designed 40 ml tube is available. For convenient handling PAA also offers a skirted, self-standing 50 ml tube. Packaged loosely in bag Cat. No. Volume (ml) Material Tube / Cap > Available in 15, 40 and 50 ml size > Excellent optical clarity and chemical resistance > Sterile packaged in bag or rack > Large field of inscription > Capable for freezing > For high speed centrifugation purposes (PAA50040 and PAA50040X) > Skirted, self-standing type (PAA50250 and PAA50250X) > Non-pyrogenic Max. Centrifuge Capacity (x g) External Dimension (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA PP / HDPE x PAA50015X 15 PP / HDPE x PAA PP / HDPE x PAA50040X 40 PP / HDPE x PAA PP / HDPE x PAA50050X 50 PP / HDPE x PAA PS / HDPE x PAA51015X 15 PS / HDPE x PAA PP / HDPE x PAA50250X 50 PP / HDPE x Tubes provided in plastic rack Cat. No. Volume (ml) Material Tube / Cap Max. Centrifuge Capacity (x g) External Dimension (mm) Sterilized Pieces / Rack Purchase Unit (pcs) PAA PP / HDPE x PAA50115X 15 PP / HDPE x PAA PP / HDPE x PAA50150X 50 PP / HDPE x PAA PS / HDPE x PAA51115X 15 PS / HDPE x PAA The Company 23

24 2-Position Tubes PAA offers 2-position tubes in two sizes to meet a wide range of different demands. Depending on the position of the snap cap an airtight closure or ventilation of the tube is achieved. The polypropylene tubes provide high chemical and thermal stability and are suitable for centrifugation at higher speed. If optical clearance is required we recommend the use of our polystyrene tubes. > Available in two sizes > High chemical and thermal stability > Two position snap cap > Suitable for centrifugation > In polypropylene (PP) and polystyrene (PS) available > Sterilized Cat. No. Volume (ml) Material Tube / Cap External Dimension (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA PS / HDPE 12 x PAA40005X 5 PS / HDPE 12 x PAA PS / HDPE 17 x PAA40014X 14 PS / HDPE 17 x PAA PP / HDPE 12 x PAA41005X 5 PP / HDPE 12 x PAA PP / HDPE 17 x PAA41014X 14 PP / HDPE 17 x Test Tubes In medicinal and biological research flow cytometry has broad applications especially in transplantation, hematology, oncology and genetics. It allows counting, examining and sorting of microscopic particles suspended in a stream of fluid. For sample preparation PAA offers polystyrene test tubes of high optical clarity. Cat. No. Volume (ml) Material External Dimension (mm) Internal Dimension (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA PS 12 x (+/ 0.1) PAA The Company

25 Microtubes, 1.5 ml and 2.0 ml Microtubes are used in every field of research and diagnostics. PAA provides microtubes made of high quality and high strength polypropylene to meet the demand of each application. Smooth opening and tight sealing as well as high chemical resistance are their main features. The high mechanical resistance permits high speed centrifugation. 2.0 ml Microtube > Temperature range -80 C to +121 C > Smooth opening and tight sealing > High chemical resistance > Autoclavable > Centrifugation up to x g > Amber color for storage of light sensitive material (PAA62015 / PAA62015X) Cat. No. Volume (ml) Color Material Sterilized Pieces / Bag Purchase Unit (pcs) PAA Transparent PP PAA60115X 1.5 Transparent PP PAA01021T 1.5 Transparent PP PAA01021Y 1.5 Yellow PP PAA01021G 1.5 Green PP PAA01021R 1.5 Red PP PAA01021B 1.5 Blue PP PAA Amber PP PAA62015X 1.5 Amber PP PAA Transparent PP PCR Tubes, 0.2 ml and 0.5 ml The polymerase chain reaction is a well-established technique to amplify DNA molecules. For this application PAA provides PCR tubes that are suitable for most major brands of thermocyclers. > High quality reaction tubes with uniformly thin wall for quick and equal temperature exchange > Applicable for standard thermocycler types > Free of DNA > Free of RNases and DNases > Autoclavable Due to their even and ultrathin well surface the PCR tubes are excellent for homogeneous and efficient heat transfer. Cat. No. Volume (ml) Color Material Sterilized Pieces / Bag Purchase Unit (pcs) PAA670305X 0.2 Transparent PP PAA Transparent PP PAA The Company 25

26 Pipette Tips Pipette tips are essential tools in molecular biology and chemistry research and diagnostic. PAA offers pipette tips made of high quality polypropylene in different sizes for various purposes. A special ionization process allows dust free tip production. The precise tips provide high quality in any detail, e.g. excellent volumetric accuracy and optimum handling due to the straight shape. This results in easy use when fitting or removing from the pipette. They are compatible with common pipette brands. The pipette tips cover the range of 0.1 µl to 10 ml and are available in bags, sterile multiracks or space-saving multitrays, where contamination free transfer to a rack is possible. > Minimum fluid retention > Excellent transparency > Compatible with most common pipettes > Autoclavable Multitray Multibox (PAA010200) with MultiTray Cat. No. Multibox for all MultiTray Pipette Tips Sample Volume (µl) Multirack Color Packaging Sterilized Pack Size (pcs / pack) Purchase Unit (pcs) PAA Transparent Bag PAA010102X Transparent Multirack x 96 PAA010103X Transparent Multirack x 96 PAA Transparent MultiTray 10 x x 96 PAA Transparent Bag PAA010112X Transparent Multirack x 96 PAA010113X Transparent Multirack x 96 PAA010114X Transparent MultiTray 2 x x 96 PAA Yellow Bag PAA010122X Yellow Multirack x 96 PAA010123X Yellow Multirack x 96 PAA Yellow MultiTray 10 x x 96 PAA Blue Bag PAA010132X Blue Multirack x 60 PAA010133X Blue Multirack x 60 PAA010134X Blue MultiTray 2 x x 60 PAA ml 10 ml Transparent Bag Cat. No. Material Packaging Sterile Purchase Unit (pcs) PAA PP Box only 1 26 PAA The Company

27 Filter Pipette Tips PAA offers also high-quality pipette tips with hydrophobic HDPE filters providing an aerosol barrier and protection against contamination of pipettes and samples in the aspiration process. They are compatible with many common pipette brands. The sterile filter tips cover a range from 0.1 µl to 1 ml and are available in racks. > Free of RNase and DNase > Minimized fluid retention > Compatible with many major pipette brands Cat. No. Sample Volume (µl) Color Packaging Sterilized Pack Size (pcs / pack) Purchase Unit (pcs) PAA010153X Transparent Rack x 96 PAA010163X 1 20 Transparent Rack x 96 PAA010173X Transparent Rack x 96 PAA010183X Transparent Rack x 96 PAA010193X Transparent Rack x 96 PAA The Company 27

28 Dispenser Dispenser and dispenser tips are implemented in the daily laboratory routine where serial working steps are required. PAA offers a dispenser for working with dispenser tips. The total Five easy-to-select settings control the aliquot volume volume, depending on the size of the tips, can be stored and after adjustment of the dispenser the precise volume can be released in single steps with a minimum aliquot volume of 1 µl. Dispensing lever designed for smooth dispensing Designed for both left and right hand operation Filling knob for easy filling of the tip Easy insertion of dispenser tip Cat. No. Purchase Unit (pcs) PAA Dispenser Tips & Dispenser Tips Professional PAA s dispenser tips and dispenser tips professional are made of high quality polypropylene and cover a range of 0.05 ml to 50 ml for various purposes. > Maximum accuracy > Free of DNase and RNase > Non-pyrogenic > Individually packed Dispenser tips are compatible with PAA s dispenser and: ripette HandyStep HandyStep electronic Multipette 4780 Distriman Lock knob for safe mounting of the tip While working with 25 ml and 50 ml tips, the use of the adapter is necessary. The dispenser tips as well as the adapter are povided sterile. Dispenser tips professional are compatible with: ripette ripette genx HandyStep HandyStep electronic Multipette 4780 Multipette plus (display will be inactive) ripette is a trademark of Ritter GmbH HandyStep is a trademark of Brand GmbH + Co. KG Multipette is a trademark of Eppendorf AG Distriman is a trademark of Gilson Inc. 28 PAA The Company

29 Dispenser Tips Cat. No. Min. Volume (µl) Max. Volume (ml) Sterilized Pieces / Bag Purchase Unit (pcs) PAA020110X PAA020111X PAA020112X PAA020113X PAA020114X PAA020115X PAA020116X Adapter necessary PAA020117X Adapter necessary PAA020120X Adapter Dispenser Tips Professional Cat. No. Min. Volume (µl) Max. Volume (ml) Sterilized Pieces / Bag Purchase Unit (pcs) PAA020210X PAA020211X PAA020212X PAA020213X PAA020214X PAA020215X PAA020216X PAA020217X Adapter included PAA020218X Adapter included PAA The Company 29

30 Multiwell Plates Multiwell plates are used in various areas of molecular biology. PAA provides multiwell plates in several sizes and for different applications. The arrangement of each well standing on its own and corresponding condensation rings on the inner side of the lid allows safer sample handling. Thus the risk of contamination from sample material being carried over is minimized. An alphanumeric well coding ensures easy sample identification. Cat. No. 1 1 U-bottom > Round bottom shape > No sharp corners for easy pipetting and cleaning > Suitable for +/ analyses 2 Well Format Material 2 F-bottom > Flat bottom shape > For precise optical measurements > For microscopic applications External Dimension (mm) Well Dimension Ø (mm) Multiwell Plates for cell culture purposes can be found on page 11. > Optically clear for microscopy > Lid with condensation rings > Safe grip for simplified handling > Stackable and space saving design > 96 well with U- or F-bottom Growth Area (cm²) Working Volume (ml) Pieces / Bag Purchase Unit (pcs) PAA31006X 6 F PS 85.4 x PAA31012X 12 F PS 85.4 x PAA31024X 24 F PS 85.4 x PAA31048X 48 F PS 85.4 x PAA31096X 96 F PS 85.4 x PAA34196X 96 U PS 85.4 x PAA The Company

31 Tray Plate Tray Plates are suitable for a multitude of applications. PAA provides a tray plate that can be used in microbiology. The rectangular shape of the tray dish provides a larger surface area compared to round type dishes and thus enables easy handling for any application. The external dimension of this tray plate is identical to standard multiwell plates and thus allows using standard HTS systems for automated application. > Clear grade polystyrene > Useful for colony counting or colony picking > Sterilized Cat. No. Square Dishes Material External Dimension (mm) Square dishes are commonly used in microbiology applications. They are designed for phage typing, agar diffusion assays, susceptibility testing, plate counts as well as traced colony picking. Internal Dimension (mm) Growth Area (cm 2 ) Sterilized Pieces / Bag The large surface area and the gridded bottom are useful for tracing the location of individual colonies. PAA offers square dishes made of polystyrene. The grid consists of thirty-six squares. > Optically clear > High plate planarity > Available with grids for cloning or determination of plating efficiency > Alphanumerical coding > Sterilized Purchase Unit (pcs) PAA31001X PS x 85.6 x x 73.5 x Cat. No. Material External Dimension (mm) Square Size (mm) Growth Area (cm 2 ) Sterilized Pieces / Bag Purchase Unit (pcs) PAA11125X PS 125 x 125 x x PAA10245X PS 245 x 245 x x PAA11245X PS 245 x 245 x x PAA The Company 31

32 Erlenmeyer Flasks Erlenmeyer flasks are one of the most commonly used tools for all purposes in laboratories, e.g. ph titrations, shaker culture applications such as microbial or plant cultures, as well as liquid handling and storage. They feature a conical flat broad bottom and a cylindrical neck. PAA offers Erlenmeyer flasks suitable for many types of suspension cultures. They are made of autoclavable polycarbonate. The flasks have a clear graduation on the side to indicate the approximate volume of the content. They are provided with a plastic cap and available in the sizes of 250 ml, 500 ml and 1000 ml. > Appropriate for shaker culture applications, liquid handling or storage > Suitable for many types of suspension cultures (E. coli, plant callus, etc.) > Half way closing enables air ventilation; full closing seals tightly > Breakage resistant > Autoclavable Cat. No. Material (Flask / Cap) Volume (ml) Purchase Unit (pcs) PAA73250 PC / PP PAA73250X PC / PP PAA73500 PC / PP PAA73500X PC / PP PAA73000 PC / PP PAA73000X PC / PP PAA The Company

33 Loops & Needles Loops & needles are convenient tools for picking isolated colonies within mixed cultures growing on plated medium. They are also highly suitable for making stab inoculations into agar slants or tubes with solid culture medium. PAA provides two different sizes of loops for individual purpos- es. Made of flexible polypropylene, the smooth loop surface allows easy streaking as well as easy handling for inoculation. Sterilization of the loops & needles reduces the contamination risk and renders flaming unnecessary. This saves valuable time for waiting of glass or metal instruments to cool down. Spreader PAA provides a convenient and cost saving cell spreader made of polypropylene for different purposes in mammalian and bacterial culture work. The L-shaped design provides fast and even sample distribution in culture dishes. Unlike glass alternatives, plastic spreaders are non-breakable and do not pose a safety hazard. 30 mm 5 mm 140 mm > For inoculation of microbes > Dual purpose: with loop and needle > Choice of inoculation volume (1 or 10 µl) > Flexible PP material > Sterilized Cat. No. Material Inoculation Volume (µl) Length (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA90001X PP PAA90010X PP Sterilization of the spreader reduces the contamination risk and renders flaming unnecessary. This saves valuable time for waiting of glass or metal instruments to cool down. The resistant material enables autoclaving and reuse. > Breakage resistant > Immediate use without flaming > Spreading of microbe sample at petri dishes > Autoclavable > Sterilized Cat. No. Material Sterilized Pieces / Bag Purchase Unit (pcs) PAA90050X PP PAA The Company 33

34 Colorimetric techniques, such as ELISA or general binding assays are used as a diagnostic tool as well as a quality control check. PAA provides different types of immunoplates for qualitative and quantitative analyses. They feature a maxi-, medi- or unibinding surface to meet every demand. The maxibinding surface is modified to provide a high binding capacity for large molecules with both hydrophobic and hydrophilic regions, such as immunoglobulins (antibody ELISA). The medibinding surface is well suited for smaller molecules with hydrophilic and hydrophobic properties (antibody / antigen ELISA). The unibinding surface is designed for binding molecules with mainly hydrophobic regions (antigen ELISA). 34 PAA The Company

35 96 Well (ELISA Plates) Strip Black & White Well Microplates PAA The Company 35

36 96 Well (ELISA Plates) are used in diagnostics and immunological research. They are suitable for colorimetric techniques, such as ELISA (Enzyme-linked Immunosorbent Assay) and provide reliable performance in binding assays when consistent coating of wells is required. PAA offers ELISA plates with a maxi- or medibinding surface, resulting in high binding capacity for proteins and other molecules with both hydrophilic and hydrophobic regions. The arrangement of each well standing on its own and raised rims allow safe sample handling. Therefore, the risk of contamination from sample material being carried over is minimized. An alphanumeric coding ensures easy sample identification. Cat. No. Material Well Format Surface Type Strip For greater flexibility in diagnostics PAA offers strip immunoplates with maxi- or medibinding surface. One strip consists of 8 wells and 12 strips fit into one frame, thus forming a standard 96 well plate. The wells of PAA38096X and PAA38196X are stabilized with a polystyrene bar and thus fixed for easy and safe handling. > Developed for immuno assays (ELISA) and general binding assays > Flat bottom type enables precise optical measurements > Alphanumerical coding > Designed for easy handling and automation > Available with maxi- or medibinding surface External Dimension (mm) Total Volume (µl) Pieces / Bag Purchase Unit (pcs) PAA32296X PS 96 F Maxibinding 85.4 x PAA32396X PS 96 F Medibinding 85.4 x Strip Type Well Type Cat. No. Material Configuration Surface Type With PAA38296X and PAA38396X the individual wells can be easily broken off separately. The number of performing tests can thus be precisely adjusted to the number of samples. The frame itself provides individual 96 wells for safe fixation of the wells. An alphanumerical coding ensures easy sample identification for both products. > Developed for immuno assays (ELISA) and general binding assays > Flat bottom type enables precise optical measurements > Raised rims on the wells > Alphanumerical coding > Designed for easy handling and automation > Available with maxi- or medibinding surface External Dimension (mm) Total Volume (µl) Pieces / Bag Purchase Unit (pcs) PAA38096X PS Strip Type Maxibinding 85.4 x PAA38196X PS Strip Type Medibinding 85.4 x PAA38296X PS Well Type Maxibinding 85.4 x PAA38396X PS Well Type Medibinding 85.4 x PAA The Company

37 Black & White (Fluoro- & Luminoplates) Colorimetric assays, e.g. fluorescence or luminescence measurements are more and more common in cell culture research. To avoid negative side effects such as background interference, autofluorescence or cross talk the use of black or white pigmented microplates is recommended. PAA provides black and white high quality 96 well immunoplates suitable for various high throughput assays. High signalto-noise ratio with low background is a characteristic feature of these plates. The surface of the plates is modified with both hydrophilic and hydrophobic regions, thus showing a maximum binding capacity. Cat. No. Material Well Format Plate / Lid Surface Type 384 Well High throughput assays and automated systems are used for the economic handling of a huge number of samples. To minimize the working volume, 384 well microplates are recommended. PAA offers high quality 384 well imunooplates in transparent, black or white, each with a transparent lid. High signal-to-noise ratio with low background is a characteristic feature of these > Available in white and black > HTS compatible > Suitable for colorimetric assay, fluorescence assay, luminescence assay > High signal-to-noise ratio > Low background interference > Maxibinding surface for high binding capacity > Provided with lid External Dimension (mm) Total Volume (µl) Pieces / Bag Purchase Unit (pcs) PAA31396X PS 96 F White / Trans. Maxibinding 85.4 x PAA31496X PS 96 F Black / Black Maxibinding 85.4 x Cat. No. Material Well Format Plate / Lid Surface Type plates. Alphanumerical well coding ensures easy sample identification. The plates are available with maxibinding surface for molecules with both hydrophobic and hydrophilic properties as well as with unibinding surface for rather hydrophobic molecules. > HTS compatible > For colorimetric, fluorescence and luminescence assays > Low Background > High signal-to-noise ratio > Transparent, black or white > Available with maxi- and unibinding surface > Provided with transparent lid External Dimension (mm) Total Volume (µl) Pieces / Bag Purchase Unit (pcs) PAA31384X PS 384 F Clear / Trans. Unibinding 85.4 x PAA32384X PS 384 F White / Trans. Unibinding 85.4 x PAA33384X PS 384 F Black / Trans. Unibinding 85.4 x PAA34384X PS 384 F Clear / Trans. Maxibinding 85.4 x PAA35384X PS 384 F White / Trans. Maxibinding 85.4 x PAA36384X PS 384 F Black / Trans. Maxibinding 85.4 x PAA The Company 37

38 Cell based imaging techniques show a rapid growth in different areas of research and development. The requirements are higher than for standard applications. PAA provides innovative products for cell based assays, fluorescence microscopy and high content screening. They feature high plate planarity, low background interference and high light transmission for challenging experiments. 38 PAA The Company

39 Cell Imaging Imaging Plates CG, with Glass Bottom Imaging Plates FC, with Fluorocarbon Film Bottom.. 42 Imaging Dishes CG (Cover Glass) Imaging Dish Stage Frames Imaging Chambers / Imaging Chambers CG Imaging Chamber Accessories Slides Slide Tray Plates CytoCapture PAA The Company 39

40 Cell Imaging Cell Imaging Surface treatment by methods of micro- and nanopatterning becomes more and more important in many applications of the life science sector. PAA provides polymer and glass products designed to support challenging cell based imaging and spectro- metry tasks. General applications are among others: Cell migration, target identification, early and secondary toxicology, functional genomics and high throughput screening. Detection technologies > Laser Scanning Microscopy > Live Cell Imaging > Differential Interference Contrast Microscopy > Total Internal Reflection Microscopy > Fluorescence Correlation Spectroscopy > Slide based technologies, slide and array manipulation The pictures below show adherent cells cultivated in Imaging Plates FC, stained and analyzed under the microscope. The gas permeable highly light transmitting bottom material (fluorocarbon) enables a special combination of excellent imaging fea- Special assays > Intracellular imaging > Control of gaseous partial pressure in cellular microenvironment > Phototoxicity studies (UVA and UVB) > Horizontal manipulation and maintenance of microscopy slides, array slides (e.g. Comet-Assay or hybridization technologies) Special cell culture requirements > Cells with high energy and oxygen requirements > Cells with sensitive adhesion > Primary cells derived from solid tissues Technology compatibility PS plate CG FC Fluorescence Intensity Fluorescence Correlation Spectroscopy* (+) + + Confocal Microscopy* and Spinning Disc Confocal Microscopy (+) + + Phase Contrast Microscopy Differential Interference Contrast + Hoffmann's Modulation Contrast Total Internal Reflection Microscopy + FRET, FRAP, FLIM, TRF, FP +/ + + Laser Scanning Cytometry *Bottom flatness of high importance PS = Polystyrene CG = Cover Glass FC = Fluorocarbon tures and support of cell physiology. Without the need for cover slips outstanding results can be achieved in fluorescence analysis and imaging directly from the multiwell plate. Fluorescent Phalloidin-TRITC (red) marking F-actin filaments and Hoechst stain (blue) marking the nucleus in HeLa cells (40x) Phase contrast image of HeLa cells (40x) 40 PAA The Company

41 Cell Imaging Imaging Plates CG, with Glass Bottom PAA offers black polymer plates (24 and 96 well) with a bottom made of borosilicate glass. The extra thin glass bottom (145 µm) combined with an excellent planarity allows imaging techniques with high resolution. Advanced surface treatment ensures optimal cell adhesion, distribution and spreading. Low evaporation lids reduce edge effects. The anticondensation treatment of the lid minimizes condensation droplet formation. The external dimensions are in compliance with the recommendations of the Society for Biomolecular Screening (SBS). Therefore, the plates are suitable for most standard automated microscopes and imaging readers. Cat. No. Well Format PAA PAA X 24 PAA PAA X 96 Applications > Total Internal Reflection Fluorescence (TIRF) > Laser Scanning Confocal Microscopy > Differential Interference Contrast (DIC) > Fluorescence Correlation Spectroscopy (FCS) Features > Excellent plate planarity (inner well flatness 10 µm) > Extra thin glass bottom (145 µm) > Imaging with high resolution > No further coating necessary > Optimal cell adhesion > No cross contamination due to anti-condensation treatment of lid > Temperature range 20 C to +50 C > Sterile > Single packaged Bottom Material Borosilicate glass Borosilicate glass Borosilicate glass Borosilicate glass Inner Well Diameter (mm) Suggested Working Volume (µl) Total Volume (µl) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA The Company 41

42 Cell Imaging Imaging Plates FC, with Fluorocarbon Film Bottom PAA offers black polymer plates (24 and 96 well) with a bottom made of high performance fluorocarbon film. The bottom of the plates is 25 µm thin. The fluorocarbon film is highly light transmitting and gas permeable thus combining excellent imaging features and support of cell physiology. Advanced surface treatment ensures optimal cell adhesion, distribution and spreading. The 96 well version is designed as low profile plate to support the use of immersion objectives for the entire plate. In addition, low evaporation lids reduce edge effects. The anticondensation treatment of the lid minimizes droplet formation. Due to a very low inner well flatness and well to well flatness fluorocarbon imaging plates now open up new dimensions of imaging techniques. The external dimensions are in compliance with the recommendations of the Society for Biomolecular Screening (SBS) and therefore suitable for the majority of standard automated microscopes and imaging readers. Cat. No. Well Format Applications > Highly metabolically active cells > Life cell imaging > High resolution imaging > Sensitive fluorescence dyes Features > Excellent plate planarity (inner well flatness 10 µm) > Extra thin gas permeable bottom (25 µm) > High performance fluorocarbon film bottom > Excellent light transmission > Low intrinsic fluorescence > No further coating necessary > Temperature range 20 C to +50 C > Sterile > Single packaged Bottom Material Inner Well Diameter (mm) Suggested Working Volume (µl) Total Volume (µl) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA Fluorocarbon PAA X 24 Fluorocarbon PAA Fluorocarbon PAA X 96 Fluorocarbon PAA The Company

43 Cell Imaging Imaging Dishes CG (Cover Glass) & Stage Frames PAA offers imaging dishes with a bottom made of cover glass for imaging purposes which are highly demanding. The material of this bottom is borosilicate glass and provides an excellent planarity and focus control for imaging techniques with high resolution. The 35 mm dishes are available with 145 µm or 175 µm glass thickness. For better microscopic orientation the 175 µm glass version is also offered with engraved micro grid. Macroscopic orientation is supported by N-O-S-W marks. A grip zone around the dish allows secure handling and enables an easy positioning of the dish and (re-)localisation of the region of interest if combined with our imaging dish stage frames. These are available in two variants: as single dish frame that holds one dish and can be used on a plane microscope or with inserts for slides and as double dish frame that can hold two imaging dishes. A stage insert for multiwell plates is necessary to use this variant. The dishes are packed in bags of six with a tray of polystyrene which can be used for better and safer transport of the dishes in the laboratory. Applications > Laser Scanning Confocal Microscopy > Total Internal Reflection Fluorescence (TIRF) > Differential Interference Contrast (DIC) > Fluorescence Correlation Spectroscopy (FCS) > Low Intensity Fluorescence Features > Extra thin glass bottom > Imaging with high resolution > Surface treatment to support cell spreading and adhesion > Easy grip for secure handling > Imaging area Ø: 18 mm > Temperature range -20 C to +50 C > Packaged in bags of six with a tray Imaging Dishes CG Cat. No. Glass Thickness (µm) µgrid Sterilized Pieces / Bag Purchase Unit (pcs) PAA PAA X PAA X PAA PAA X PAA X PAA PAA X PAA X Imaging Dish Stage Frame Cat. No. Frame Type Outer Dimensions (mm) Sterilized Pieces / Bag Purchase Unit (pcs) PAA Single 52 x PAA Double x PAA The Company 43

44 Cell Imaging Imaging Chambers / Imaging Chambers CG For convenient handling PAA offers Imaging Chambers that allow culturing, staining and fixation of cells inside the same vessel. They are available with cover glass (CG) or microscope slides as bottom structure. Both types are available in 1 well, 2 well, 4 well and 8 well format. Imaging Chambers CG are specially designed for high resolution live cell microscopy techniques. Imaging Chamber with microscope slides can be used with long distance objectives. PAA`s Imaging Chambers and lids are made of high performance polymers with better chemical resistance and temperature stability than the commonly used polystyrene. The chambers are sealed against the glass bottoms by biologically inert silicone adhesive. At the end of the experiments the glasses can be easily detached from the polymer chambers with minimal glue residue. Cover Glass Microscope Slide Applications > Immunohistology > Total Internal Reflection Fluorescence (TIRF) > Fluorescence Resonance Energy Transfer (FRET) > Differential Interference Contrast (DIC) > Fluorescence In-Situ Hybridization (FISH) > Long-term storage Features > Easy removal of growth chamber > Biologically inert glue > Great chemical resistance (e.g. acetone) > High temperature stability up to +90 C > Outstanding cell adhesion due to plasma treatment Cat. No. Format Bottom Type Growth Area (cm 2 ) Working Volume (ml) Sterilized Pieces / Bag Purchase Unit (pcs) PAA X 16 1 well Cover Glass PAA X 80 PAA X 16 2 well Cover Glass PAA X 80 PAA X 16 4 well Cover Glass PAA X 80 PAA X 16 8 well Cover Glass PAA X 80 PAA X 16 1 well Microscope Slide PAA X 80 PAA X 16 2 well Microscope Slide PAA X 80 PAA X 16 4 well Microscope Slide PAA X 80 PAA X 16 8 well Microscope Slide PAA X PAA The Company

45 Cell Imaging Imaging Chamber Accessories PAA provides a special lid with borosilicate glass insert as alternative to the standard polymer lid in order to improve the results during differential interference contrast (DIC) microscopy. The lids are sterilized and packed in bags of four. For the extension of the Imaging Chambers CG to full length of standard microscope slides PAA offers an optional adapter. This allows the usage of Imaging Chambers CG with regular microscope stage inserts for microscope slides. The adapter is made of stainless steel and can be reused. Cat. No. Produkt Sterilized Pieces / Bag Purchase Unit (pcs) PAA Imaging Chamber DIC Lid PAA Imaging Chamber Adapter 1 1 PAA The Company 45

46 Cell Imaging Slides PAA offers 4 well and 8 well cell culture slides that enable culturing, staining and fixation of cells in the same vessel. The chambers can easily be detached from the microscope slide bottom without any glue residues. In addition to cell culture slides with a transparent chamber, they are also available with black or white chamber for colorimetric assays. The cell culture slides are packed in trays of six pieces, sealed individually. Thus, sterility is preserved when only a single slide is needed. Cat. No. Chamber Format Features > Easy separation of chamber and slide > No glue residues > Leak-proof > Black or white chamber for colorimetric assays > Sterilized > Non-pyrogenic Chamber Color Growth Area (cm 2 ) Working Volume (ml) Sterilized Pieces / Bag Purchase Unit (pcs) PAA30104X 4 well Clear PAA30114X 4 well Black PAA30124X 4 well White PAA30108X 8 well Clear PAA30118X 8 well Black PAA30128X 8 well White PAA The Company

47 Cell Imaging Slide Tray Plates Slide Tray Plates are a convenient solution if you have to handle your microscope slides in horizontal orientation, e.g. for cell staining, chromosome preparation or Comet-Assay analysis. The PAA slide tray plates are available in two different materials. Polystyrene (PS) is crystal clear and sterile which is the prerequi- site for the cultivation of cells. Polypropylene (PP) on the other hand features a better chemical resistance towards reagents such as acetone. The plates can handle up to four slides in parallel. > Easy handling > Easy access zone for slide removal > Transparent PS or chemical resistant PP > For most standard slides > Parallel testing > Stackable Cat. No. Material Single Chamber Width / Length (mm) Surface Treated Sterilized Pieces / Bag Purchase Unit (pcs) PAA PS 28 x PAA X PS 28 x PAA PS 28 x PAA X PS 28 x PAA PP 28 x PAA X PP 28 x Innovative product line for Cytogenetics Cytogenetics is the study of chromosomes and the related diseases caused by numerical and structural chromosome abnormalities. Chromosome studies are important diagnostic procedures in prenatal and postnatal diagnosis. PAA offers a product line including special media, serum and reagents for the cultivation of amniocytes, chorionic villi cells and lymphocytes. These products support you to achieve reliable results for further medical interventions. Read more about PAA s Cytogenetic Products at PAA The Company 47

48 Cell Imaging CytoCapture It is very challenging to trace non adherent cells under the microscope due to the prevailing fluid dynamics. For experiments that require the separation and arrest of living cells or spheroids PAA offers a variety of microscopy labware with microscale cavities. These CytoCapture structures promote the spontaneous sedimentation of cells and protect them against fluid movements. Media exchange as well as staining or fixation can be performed while the cells reside within their cavities. CytoCapture products are also well-suitable for 3D cultivation and imaging of individual adherent cells. Cavity geometry Imaging Dish CG Applications > Time lapse analysis of suspension cells > Life cell staining > Culture and analysis of spheroids > 3D cultivation of adherent cells > Immunohistochemistry Labware Format Microscope Slide Available combinations of cavity geometry and labware format: Imaging Chamber Features > Spontaneous sedimentation of cells in single cavities > Maintenance of cell location during treatments > Well selected glass bottoms for high resolution microscopy Imaging Dish CG Microscope Slide Imaging Chamber Small hexagonal Square Big hexagonal + Big hexagonal Low cell adhesion Cavity geometry Small hexagonal Square Big hexagonal Best suitable for: + + Capturing suspension cells with a diameter below 15 µm or cell nuclei Capturing larger suspension cells and for 3D guided cultivation of adherent cells Clustering up to 100 adherent or suspension cells per microcavity Big hexagonal Low cell adhesion Controlled formation and investigation of cellular spheroids CytoCapture is a joint development of Molecular Cytomics Inc. and zell-kontakt GmbH. 48 PAA The Company

49 Cell Imaging CytoCapture: Small Hexagonal Cavities For convenient imaging of suspension cells PAA offers CytoCapture products with small hexagonal microscale cavities. This geometry is best suitable for trapping small individual cells and protecting them against fluid movements during treatments such as media exchange, life staining of cells or intracellular structures and immunohistochemistry. The single cells can be monitored at different time intervals to investigate the response to specific experimental procedures. Cat. No. Labware Type Cavity Geometry Cavity Width (µm) > Hexagonal structure for capturing single cells > Available in three different labware types > Suitable for suspension cells < 15 µm > Cavity size: 20 µm diameter, 10 µm depth, 3 µm wall thickness Working Volume Low Cell Adhesion 30 µm Sterilized Pieces / Bag Purchase Unit (pcs) PAA Dish Hexagonal Ø µl (1.5 ml)* PAA X Dish Hexagonal Ø µl (1.5 ml)* PAA Slide Hexagonal Ø µl PAA X Slide Hexagonal Ø µl PAA Chamber Hexagonal Ø µl PAA X Chamber Hexagonal Ø µl * Seed cells in 50 µl and fill up to 1.5 ml. PAA The Company 49

50 Cell Imaging CytoCapture: Square Cavities For the capture and imaging of larger single cells PAA offers CytoCapture products with square cavities. This type of geometry is especially designed for 3D guided cultivation of adherent cells (e.g. epithelial cells). The upright growth of the cells allows a more natural cell development with both apical and basolateral membrane sides. The microstructure assisted control of cell morphology is a great basis for complex cell-cell interaction studies or co-culture experiments. Cat. No. Labware Type Cavity Geometry > Square structure for capturing single cells > Available in three different labware types > Suitable for adherent and suspension cells > Cavity size: 40 µm side length, 15 µm depth, 3 µm wall thickness Cavity Width (µm) Working Volume Low Cell Adhesion Sterilized Pieces / Bag Purchase Unit (pcs) PAA Dish Square 40 x µl (1.5 ml)* PAA X Dish Square 40 x µl (1.5 ml)* PAA Slide Square 40 x µl PAA X Slide Square 40 x µl PAA Chamber Square 40 x µl PAA X Chamber Square 40 x µl * Seed cells in 50 µl and fill up to 1.5 ml. 50 PAA The Company

51 Cell Imaging CytoCapture: Big Hexagonal Cavities Investigation of cell-cell interactions between suspension cells is a particular challenge. PAA offers CytoCapture products with big hexagonal cavities to facilitate experiments where a controlled clustering and analysis of cells is required. These are also available with low adhesion surface, which contributes to an enforced aggregate and spheroid formation even of adherent cells. With the CytoCapture products it is possible to change media, add substances and manipulate cells or spheroids without interference in their location. Thus, the region of interest can be kept under control allowing also time dependent experiments. Cat. No. Labware Type Cavity Geometry > Hexagonal structure for capturing up to 100 cells > Suitable for adherent and suspension cells > Spontaneous sedimentation and clustering of cells in cavities > Low adhesion surface especially for spheroid cultivation > Enables uniform formation of embryoid bodies > Cavity sizes: 250 µm diameter, 100 µm depth, 5 µm wall thickness 500 µm diameter, 100 µm depth, 10 µm wall thickness Cavity Width (µm) Working Volume Low Cell Adhesion Sterilized Pieces / Bag Purchase Unit (pcs) PAA Dish Hexagonal Ø µl (1.5 ml)* PAA X Dish Hexagonal Ø µl (1.5 ml)* PAA Dish Hexagonal Ø µl (1.5 ml)* PAA X Dish Hexagonal Ø µl (1.5 ml)* PAA Chamber Hexagonal Ø µl PAA X Chamber Hexagonal Ø µl PAA Dish Hexagonal Ø µl (1.5 ml)* PAA X Dish Hexagonal Ø µl (1.5 ml)* PAA Chamber Hexagonal Ø µl PAA X Chamber Hexagonal Ø µl * Seed cells in 50 µl and fill up to 1.5 ml. PAA The Company 51

52 DNA, RNA and protein containing material must be properly handled and stored. The same goes for freezing of mammalian cells and their long term storage in liquid nitrogen. Therefore, PAA provides high quality racks and storage boxes also for use in cryo conservation to meet different kinds of applications. 52 PAA The Company

53 Handling & Storage Cryotubes Inserts for Cryotubes Working Rack for Cryotubes Cryo Boxes Storage Boxes Storage Boxes with Hinged Cover Conical Centrifuge Tube Racks Microtube Racks PCR Tube Racks Reservoirs Square Bottles Autoclave Bags PAA The Company 53

54 Handling & Storage Cryotubes Excellent cooling and freezing conditions with PAA s Cell Preservation Products There are two common methods of cell preservation. Hypothermic preservation at temperatures above freezing allows short term storage of tissues and organs. Cryogenic preservation at temperatures below freezing is used for the establishment of cell banks to ensure reproducible results in research by using stable cell strains. All PAA cell preservation products provide outstanding cooling and freezing conditions with maximum viability and recovery after warming. Learn more about PAA s Cell Preservation Products at Cryotubes are commonly used for storage of cells, sera, blood components, bacteriological cultures and other fluids at low temperature. The cryotubes of PAA are made of clear high grade polypropylene. Using the same material for both vial and lid as well as the exclusion of an O-ring reduce the risk of explosion during thawing to a minimum. The round bottom shape facilitates complete transfer of the cell material to the cell culture flask or dish. The starfoot base of the tubes enables easy handling within a working rack. The cryotubes are suitable for storage in refrigerators or in the gas phase of liquid nitrogen as the material is stable at temperatures down to -196 C. > Safe storage of biological material > White printed graduation and large writing area > Colored inserts available for easy sample identification > External thread > Easy to handle in one-hand operation within the working rack > Free of DNase / RNase > Sterile > Non-pyrogenic Cat. No. Material Volume (ml) Pieces / Bag Purchase Unit (pcs) PAA PP PAA535001X PP PAA PP PAA535002X PP PAA The Company

55 Handling & Storage Inserts for Cryotubes For easy sample identification PAA provides fitting cap inserts in five different colors. Cat. No. Color Material Pieces / Bag Purchase Unit (pcs) PAA colors (Blue, White, Yellow, Violet, Red) PP x 100 PAA535050B Blue PP PAA535050W White PP PAA535050Y Yellow PP PAA535050V Violet PP PAA535050R Red PP Working Rack for Cryotubes PAA offers an appropriate support rack suitable for cryotubes with a starfoot base. This system eases the handling of the tubes with one hand. The rack provides space for up to 40 cryotubes. Cat. No. Material Array (holes) Purchase Unit (pcs) PAA PC 4 x 10 1 PAA The Company 55

56 Handling & Storage Cryo Boxes The cryo boxes are made of clear polycarbonate. They are designed for the handling and storage of 1.2 ml and 2 ml cryotubes to be used at temperatures ranging from -196 C to +121 C. The numbering on the transparent lid of the box allows a quick and easy inventory control. The boxes are available in three different colors (yellow, blue, red) and in two different sizes (25 and 81 positions). > Temperature range: -196 C to +121 C > Enhanced draw off of liquid nitrogen > Clear numbered lid > Two different sizes > Three different colors > Material: clear polycarbonate (PC) Cat. No. Color Material External Dimension (mm) Array (holes) Purchase Unit (pcs) PAA80025Y Yellow PC 76 x 76 x 53 5 x 5 1 PAA80025B Blue PC 76 x 76 x 53 5 x 5 1 PAA80025R Red PC 76 x 76 x 53 5 x 5 1 PAA80081Y Yellow PC 132 x 132 x 53 9 x 9 1 PAA80081B Blue PC 132 x 132 x 53 9 x 9 1 PAA80081R Red PC 132 x 132 x 53 9 x PAA The Company

57 Handling & Storage Storage Boxes The PAA storage boxes are made of polypropylene and prove to be an excellent option for storage and handling of 1.2 ml and 2 ml cryotubes and 1.5 ml microtubes in the laboratory. They are intended for use in a temperature range of -80 C to +121 C. The storage boxes are available with different lids and colors. Storage Box with alphanumerical coding on lid and bottom For the storage of 1.2 and 2 ml cryotubes, 1.5 ml microtubes > Temperature range -80 C to +121 C > For 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes > Temperature range -80 C to +121 C > For 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes > Different colors available Cat. No. Color Material External Dimension (mm) Array (holes) Purchase Unit (pcs) PAA535055B Blue PP 130 x 130 x 54 9 x 9 1 PAA535055G Green PP 130 x 130 x 54 9 x 9 1 PAA535055R Red PP 130 x 130 x 54 9 x 9 1 PAA535055Y Yellow PP 130 x 130 x 54 9 x 9 1 PAA535055T Transparent PP 130 x 130 x 54 9 x 9 1 Storage Box with numerical coding on inlay For the storage of 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes Cat. No. Color Material External Dimension (mm) Array (holes) Purchase Unit (pcs) PAA81081 Transparent PP 130 x 130 x 51 9 x 9 1 PAA81081X Transparent PP 130 x 130 x 51 9 x 9 40 PAA The Company 57

58 Handling & Storage Storage Boxes with Hinged Cover The PAA storage boxes with hinged cover are made of polypropylene and prove to be an excellent option for storage and handling of 1.2 ml cryotubes and 1.5 ml microtubes in the laboratory. They are intended for use in a temperature range of -80 C to +121 C. The storage boxes are available with different lids and colors. The boxes can be sterilized by autoclaving. Storage Box with alphanumerical coding on hinged cover For the storage of 1.2 ml cryotubes and 1.5 ml microtubes > Temperature range -80 C to +121 C > Autoclavable > For 1.2 ml cryotubes and 1.5 ml microtubes > Different colors available Cat. No. Color Material External Dimension (mm) Array (holes) Purchase Unit (pcs) PAA80100 Transparent PP 130 x 146 x x 10 1 PAA80100X Transparent PP 130 x 146 x x PAA81100G Green PP 130 x 146 x x 10 1 PAA81100V Violet PP 130 x 146 x x 10 1 PAA81100O Orange PP 130 x 146 x x 10 1 PAA81100P Pink PP 130 x 146 x x 10 1 PAA81100B Blue PP 130 x 146 x x PAA The Company

59 Handling & Storage Conical Centrifuge Tube Racks In the daily working routine conical centrifuge tubes are often used in the laboratory. PAA offers conical tube racks that are suitable for handling and storage of 25 conical tubes (15, 40 or 50 ml). The folded racks are a cost saving alternative to stainless wire racks or other plastic racks. They are easy to assemble and made of polypropylene. > Handling and storage of 25 conical tubes > Special position circles ensure safe stand > Autoclavable > Three colors available Cat. No. Color Material Tube Size (ml) Description Purchase Unit (pcs) PAA52015R Red PP holes 1 PAA52015B Blue PP holes 1 PAA52015G Green PP holes 1 PAA52050R Red PP 40, holes 1 PAA52050B Blue PP 40, holes 1 PAA52050G Green PP 40, holes 1 PAA The Company 59

60 Handling & Storage Microtube Racks Microtube racks are excellent tools for the preparation of samples. PAA offers two different types of racks. The classical one is made for handling and storage of up to 80 microtubes. The double sided one alternatively provides space for 0.2 ml microtubes. Both racks are provided with a numerical coding for easy tube identification. > For handling and storage of up to 80 microtubes > 5 different colors Cat. No. Color Material Array (holes) Purchase Unit (pcs) PAA61080W White PP 5 x 16 1 PAA61080R Red PP 5 x 16 1 PAA61080B Blue PP 5 x 16 1 PAA61080G Green PP 5 x 16 1 PAA61080V Violet PP 5 x 16 1 Double sided Microtube Rack For handling of either 60 microtubes 0.2 ml or 80 microtubes 1.5 ml > Two usable sides > For 60 microtubes 0.2 ml or 80 microtubes 1.5 ml > Autoclavable > 4 different colors Cat. No. Color Material Array (holes) Purchase Unit (pcs) PAA010302B Blue PP 5 x 16 or 4 x 15 1 PAA010302G Green PP 5 x 16 or 4 x 15 1 PAA010302R Red PP 5 x 16 or 4 x 15 1 PAA010302Y Yellow PP 5 x 16 or 4 x PAA The Company

61 Handling & Storage PCR Tube Racks The polymerase chain reaction (PCR) and its variants are standard techniques widely used in the field of molecular biology. Usually PCR reactions are prepared in 0.2 ml microtubes and carried out in a universal PCR cycler with adequate positions. The PCR tube rack of PAA is an outstanding tool for handling, preparation and reactions of small volumes. With its lid and the stack able design it is also useful for storage of samples at room temperature or in the refrigerator. Made of polypropylene our PCR tube rack is fully autoclavable. > For 0.2 ml microtubes > Suitable for PCR strips and plates > With lid > Stackable > Autoclavable > 5 different colors Cat. No. Color Material Array (holes) Purchase Unit (pcs) PAA80096W White PP 8 x 12 1 PAA80096R Red PP 8 x 12 1 PAA80096B Blue PP 8 x 12 1 PAA80096G Green PP 8 x 12 1 PAA80096V Violet PP 8 x 12 1 PAA The Company 61

62 Handling & Storage Reservoirs Various experiments in the laboratory require serial pipetting steps. For more convenience PAA offers disposable pipetting reservoirs made of white polystyrene. The reservoirs are de- signed for the use with single or multichannel pipettes. In order to avoid contaminations they are supplied sterile and individually packaged. > Suitable for single and multi channel pipettes > Notched volume graduation (PAA22050X) > Individually packed > Sterilized Cat. No. Wells Total Volume (ml) Material Sterilized Pieces / Bag Purchase Unit (pcs) PAA22050X 1 50 PS PAA21002X 2 25 PS PAA21008X 8 7 PS PAA21012X 12 4 PS PAA The Company

63 Handling & Storage Square Bottles PAA offers a sterile square PET bottle in three different sizes for various applications. The bottles meet current biological testing standards. They are suitable for preserving and transporting sensitive liquids for biopharmaceutical applications. The bottles are extremely lightweight, shatter-resistant and provide excellent gas barrier properties. The bottles are delivered in brickpacks. Two or four brickpacks are double bagged and then packed in master boxes. Brickpack > Available in sizes of 125, 500 and 1000 ml > Chemically and biologically inert > Shatter-resistant and lightweight > Gas impermeable > Clearly transparent > Sterilized Cat. No. Volume (ml) Material Sterilized Pieces / Brickpack Brickpacks / Box Purchase Unit (pcs) PAA56125X 125 PET PAA56500X 500 PET PAA56000X 1000 PET PAA The Company 63

64 Handling & Storage Autoclave Bags Working with biohazardous materials requires correct handling. In addition, the contaminated laboratory disposals like cell culture flasks or pipette tips have to be destroyed in an appropriate way before final removal. PAA offers autoclave bags made of particular strong polypropylene film to use during daily laboratory routine. For autoclaving the bags should not be closed completely. They are autoclav- able up to 134 C and available as standard as well as highly transparent bags. In addition to the autoclave bags PAA offers a convenient holder, which can be used on the bench and eases the handling with contaminated biohazard material. This plastic coated holder has three non-slip rubber feet and is suitable for bag size 200 x 300 mm. > PP bags made of particularly strong film (film thickness 50 µm) with a wide bottom seam > For disposal of contaminated laboratory items > Autoclavable up to +134 C > For autoclaving, the bag should be closed slightly > Two different types available Cat. No. Width x Length (mm) Volume (l) Max. Autoclave Temperature Pieces / Bag Purchase Unit (pcs) PAA x C (spender box) PAA x C PAA x C (spender box) PAA x C Autoclave Bag Holder Suitable for PAA and PAA Cat. No. Purchase Unit (pcs) PAA PAA The Company

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67 Appendix Alphabetical Index Material Properties Chemical Resistance of Various Materials PAA The Company 67

68 Appendix Alphabetical Index Product Name Page 2-Position Tubes Well (ELISA Plate) Well Microplates for Colorimetric Assays Well A B Autoclave Bag Holder Autoclave Bags Black & White Blood Separation Tubes C-Chip Neubauer Improved / Malassez Dishes Flasks Plates with Inserts Slides Cell Lifter C Cell Scrapers Center Well Culture Dish Confocal Dishes Conical Centrifuge Tubes Conical Centrifuge Tube Racks Coverslips Cryo Boxes Cryotubes CytoCapture D E Dispenser Dispenser Tips ELISA Plates (96 Well ) Erlenmeyer Flasks F Filter Pipette Tips PAA The Company

69 Appendix Alphabetical Index Product Name Page Imaging Chamber Accessories Imaging Chambers I Imaging Chambers CG Imaging Dishes CG (Cover Glass) Imaging Dishe Stage Frames Imaging Plates CG, with Glass Bottom Imaging Plates FC, with Fluorocarbon Film Bottom Inserts for Cryotubes L Loops & Needles Microtube Racks M Microtubes, 1.5 ml Microtubes, 2.0 ml Multiwell Plates Multiwell Tissue Culture Plates P PCR Tubes PCR Tube Racks Petri Dishes Pipette Tips R Reservoirs Serological Pipettes Slide Tray Plates S Spreader Square Bottles Square Dishes , 31 Storage Boxes , 58 Strip T Test Tubes Tray Plate , 31 W Working Rack for Cryotubes PAA The Company 69

70 Appendix Material Properties Polypropylene Polystyrene Polyethylene Letter symbol PP PS HDPE (High Density PE) Heat resistance Permanent loading: C ( F) Short-term max: C ( F) Suitable for use in autoclaves: 121 C (250 F) Permanent loading: C ( F) Short-term max: C ( F) Unsuitable for use in autoclaves Permanent loading: C ( F) Short-term max: C ( F) Unsuitable for use in autoclaves Low-temperature resist. (tested at) -192 C (-314 F) -30 C (-22 F) -40 C (-40 F) Flammability combustible combustible combustible Density 0.90 g / cm g / cm g / cm 3 Moisture absorption < 0.1 % < 0.1 % < 0.1 % Optical properties translucent shiny surface transparent, shiny surface with 90 % transparency index (at nm) translucent to opaque General mechanical properties High fracture strength; dimensionally stable; high stiffness, strong and hard Low fracture ductility; low warm strength; excellent electrical insulation properties; not suitable for use at high centrifugal loads Rather low tensile strength and surface hardness; high ductility at soft and harder levels; low resistance to stress cracking; water repellent; susceptible to electrostatic charging General chemical properties > High resistance to aqueous solutions of inorganic salts, acids, and alkalis, as well as to organic solvents up to 60 C > Stable in alcohols, esters and ketones > Aromatic and halogenated hydrocarbons, oxidizing substances such as concentrated nitric acid and, at higher temperatures, fats, oils and waxes all lead to swelling of PP > High resistance to salt and caustic solutions, non-oxidizing acids, alkalis and alcohols. > Petroleum spirit, etheric oils, strongly oxidizing reagents and aromatics attack PS and give rise to stress cracking > Exhibits high resistance; diluted acids, alkalis, alcohol, oil and salt solutions do not attack PE > Concentrated oxidizing acids such as nitric acid, and halogens lead to disintegration Disposal Standards PE / PP / PS are pure hydrocarbon compounds and therefore neutral to the environment. By regulated incineration no harmful substances are produced. The HDPE / PP / PE material used fulfills the requirements of Pharmacopoeia (USP) Class 6 and Class 1 medical devices according to Directive 93/ PAA The Company

71 Appendix Chemical Resistance of Various Materials The Chemical Resistance Chart below is a general guide only. Many factors can affect the chemical resistance of a given product, therefore you should test under your own conditions and always follow correct laboratory safety procedures. Caution: Do not store strong oxidizing agents in plastic labware! Resistance test conditions at +20 C; the second to those at +50 C. Chemicals 1,2 Dichloroethane 1,2,4-Trichlorobenzene 1,4-Dioxane 2,2,4-Trimethylpentane 2,4 Dichlorophenol 2-Butanol 2-Methoxyethanol 2-Propanol Acetaldehyde Acetamide, Sat. Acetic Acid, 5% Acetic Acid, 50% Acetic Acid, Glacial Acetic Anhydride Acetone Acetonitrile Acetophenone Acrylonitrile Adipic Acid Allyl Alcohol Aluminum Hydroxide Amino Acids Ammonia Ammonia, 25% Ammonium Glycolate Ammonium Hydroxide, 30% Ammonium Hydroxide, 5% Ammonium Oxalate Ammonium Salts Amyl Alcohol Amyl Chloride Aniline Aqua Regia Arsenic Acid Benzaldehyde Benzenamine Benzene Benzoic Acid, Sat. Benzyl Acetate Benzyl Alcohol Boric Acid Bromine Bromobenzene Bromoform Butadiene Butyl Acetate Butyl Chloride Butyric Acid High Density PE HDPE Polypropylene PP Polycarbonate PC Polystyrene PS No damage after 30 days of constant exposure Little or no damage after 30 days of constant exposure Some effect after 7 days of constant exposure Immediate damage may occur; not recommended for continuous use - Not measured PAA The Company 71

72 Appendix Chemicals Calcium Hydroxide Calcium Hypochlorite Carbazole Carbon Disulphide Carbon Tetrachloride Cellosolve Acetate Chlorine Water Chlorine, 10% (Moist) Chlorine, 10% in air Chlorine, wet gas Chloroacetic Acid Chlorobenzene Chloroform Chromic Acid, 10% Chromic Acid, 20% Chromic Acid, 50% Chromic:Surfuric Acid Mixture, 96% Citric Acid, 10% Cresol Cyclohexane Cyclohexanone Cyclopentane Decahydronaphtalene Diacetone Diacetone Alcohol Dibutylphthalate - Diethyl Benzene Diethyl Ether Diethyl Ketone Diethyl Malonate Diethylamine Diethylene Dioxide Diethylene Glycol Diethylene Glycol Ethyl Ether Dimethyl Acetamide Dimethyl Formamide Dimethylsulphoxide (DMSO) Dioxane Dipropylene Glycol Ethanol, 40% Ether Ethyl Acetate Ethyl Alcohol (Absolute) Ethyl Alcohol, 40% Ethyl Alcohol, 96% Ethyl Benzene Ethyl Benzoate Ethyl Butyrate Ethyl Chloride Ethyl Chloride, Liquid Ethyl Cyanoacetate Ethyl Lactate Ethylene Chloride Ethylene Glycol Ethylene Glycol Monomethyl Ether Ethylene Oxide Gas Ethylene Oxide, 100% High Density PE HDPE Polypropylene PP Polycarbonate PC Polystyrene PS No damage after 30 days of constant exposure Little or no damage after 30 days of constant exposure Some effect after 7 days of constant exposure Immediate damage may occur; not recommended for continuous use - Not measured 72 PAA The Company

73 Appendix Chemicals Fatty Acids Fluorine Formaldehyde, 10% Formaldehyde, 40% Formalin, 10% Formalin, 40% Formic Acid Formic Acid, 100% Formic Acid, 3% Formic Acid, 50% Formic Acid, 85% Freon TF Glutaraldehyde Glycerine (Glycerol) Hexane Hydrazine Hydrobromic Acid, 4% Hydrobromic Acid, 48% Hydrobromic Acid, 69% - Hydrochloric Acid, 20% Hydrochloric Acid, 35% Hydrochloric Acid, 5% Hydrogen Peroxide, 3% Hydrogen Peroxide, 30% Hydrogen Peroxide, 90% Isobutanol Isopropanol, 100% Isopropyl Acetate Isopropyl Benzene Isopropyl Ether Lactic Acid, 3% Lactic Acid, 85% Lodine Crystals Mercury Methanol, 100% Methoxyethyl Oleate Methyl Acetate Methyl Ethyl Ketone Methyl Isobutyl Ketone (MIBK) Methyl Propyl Ketone Methylene Chloride Methyl-t-Butyl Ether n-amyl Acetate n-butanol n-butyl Acetate n-decane n-heptane Nitric Acid, 10% Nitric Acid, 20% Nitric Acid, 50% Nitric Acid, 70% Nitrobenzene Nitromethane n-octane o-dichlorobenzene Oil, Mineral Oxalic Acid, 10% Ozone High Density PE HDPE Polypropylene PP Polycarbonate PC Polystyrene PS No damage after 30 days of constant exposure Little or no damage after 30 days of constant exposure Some effect after 7 days of constant exposure Immediate damage may occur; not recommended for continuous use - Not measured PAA The Company 73

74 Appendix Chemicals p-chloroacetophenone p-dichlorobenzene Perchloric Acid Perchloric Acid, 70% Perchloroethylene Phenol, 100% Phenol, 50% Phenol, Crystals Phenol, Liquid Phosphoric Acid, 5% Phosphoric Acid, 85% Picric Acid Potassium Hydroxide, 1% Potassium Hydroxide, 30% Potassium Hydroxide, Concentrated Potassium Permanganate Propane Gas Proprionic Acid Propylene Glycol Propylene Oxide Pyridine Resorcinol, 5% Resorcinol, Sat. Salicylaldehyde Salicylic Acid, Sat. Salt Solutions, Metallic Silicone Oil Silver Nitrate Sodium Dichromate Sodium Hydroxide, 50% Sodium Hydroxide, 1% Sodium Hydroxide, 10% Sodium Hypochlorite, 15% Stearic Acid Sulfur dioxide Sulfur Dioxide, wet or dry Sulfur Salts Sulfuric Acid, 96% Sulfuric Acid, 20% Sulfuric Acid, 30% Sulfuric Acid, 6% Sulfuric Acid, 60% Sulfuric Acid, 98% Tartaric Acid Tetrahydrofuran Thionyl Chloride Tincture of Iodine Toluene Tributyl Citrate Trichloroacetic Acid (TCA) Trichloroethane Trichloroethylene Triethylene Glycol Tripropylene Glycol Tris Buffer, Solution Urea Xylene High Density PE HDPE Polypropylene PP Polycarbonate PC Polystyrene PS No damage after 30 days of constant exposure Little or no damage after 30 days of constant exposure Some effect after 7 days of constant exposure Immediate damage may occur; not recommended for continuous use - Not measured 74 PAA The Company

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